Abstract:
:Protein splicing technology harnesses the ability of inteins to ligate protein fragments, forming a mature protein. This report describes our effort to engineer rapamycin-dependent protein splicing of a ribotoxin, called α-sarcin. Engineering this system required the investigation of important splicing parameters, including extein context and splicing temperature. We show α-sarcin splicing is dependent on rapamycin, is inducible with rapid kinetics, and triggers apoptosis in HeLa cells. These findings establish a proof-of-concept for a conditional cell ablation strategy.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Alford SC,O'Sullivan C,Obst J,Christie J,Howard PLdoi
10.1039/c3mb70387hsubject
Has Abstractpub_date
2014-04-01 00:00:00pages
831-7issue
4eissn
1742-206Xissn
1742-2051journal_volume
10pub_type
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