Abstract:
:The importance of microRNA (miRNA) in modulating gene expression at the post-transcriptional level is well known. Such regulation has been shown to influence the dynamics of several regulatory networks including the cell cycle. In this study we incorporated regulatory effects of intronic miRNA into an existing mathematical model of the cell cycle through the use of an existing 'proxy' protein--the host protein. It was observed that the incorporation of intronic miRNA mediated regulation improved the performance of the model resulting in a closer match to experimental results. To test the universality of this approach we compared the effects of intronic miRNA mediated regulation and host protein mediated regulation. Further, we compared miRNA mediated and protein mediated positive and negative feedback regulations of the target protein. We found that the target protein profiles were predominantly similar. These observations show the applicability of our method for incorporating intronic miRNA mediated dynamic effects in models for regulation of gene expression.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Gokhale S,Hariharan M,Brahmachari SK,Gadgil Cdoi
10.1039/c2mb25046bsubject
Has Abstractpub_date
2012-08-01 00:00:00pages
2145-52issue
8eissn
1742-206Xissn
1742-2051journal_volume
8pub_type
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