Abstract:
:Many methods have been published by which combinatorial libraries may be screened for compounds capable of manipulating the function(s) of a target protein. One of the simplest approaches is to identify compounds in a library that bind the protein of interest, since these binding events usually occur on functionally important surfaces of the protein. These protein-binding compounds could also be of utility as protein capture agents in the construction of protein-detecting microarrays or related analytical devices and as reagents for the affinity purification of proteins from complex mixtures. In this article, we provide optimized methods for screening libraries of molecules displayed on the beads on which they were synthesized. This is a particularly convenient format for library screening for laboratories with limited budgets and modest robotics capabilities.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Kodadek T,Bachhawat-Sikder Kdoi
10.1039/b514349gsubject
Has Abstractpub_date
2006-01-01 00:00:00pages
25-35issue
1eissn
1742-206Xissn
1742-2051journal_volume
2pub_type
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