Systematic screens for human disease genes, from yeast to human and back.

abstract：:Induced hypoxia stress on cervical cancer derived cells (HeLa cells) leads to significant changes in their membrane lipid profiles. The lipidome of HeLa cells was characterized by a joint approach wherein liquid chromatography-mass spectrometry (LC-MS) analysis was followed by high resolution NMR measurements. Multiva...

journal_title：Molecular bioSystems

authors： Yu Y,Vidalino L,Anesi A,Macchi P,Guella G

更新日期：2014-04-01 00:00:00

abstract：:Here we present a novel statistical methodology that allows us to analyze gene expression data that have been collected from a number of different cases or conditions in a unified framework. Using a Bayesian nonparametric framework we develop a hierarchical model wherein genes can maintain a shared set of interactions...

journal_title：Molecular bioSystems

authors： Thorne T,Fratta P,Hanna MG,Cortese A,Plagnol V,Fisher EM,Stumpf MP

更新日期：2013-07-01 00:00:00

abstract：:Recently, more and more research has shown that microRNAs (miRNAs) play critical roles in the development and progression of various diseases, but it is not easy to predict potential human miRNA-disease associations from the vast amount of biological data. Computational methods for predicting potential disease-miRNA a...

journal_title：Molecular bioSystems

authors： Chen X,Liu MX,Yan GY

更新日期：2012-10-01 00:00:00

abstract：:As the usage of fluorescence microscopy as a tool to study biological systems continues to grow, so does the need for additional tools that permit the selective detection of proteins of interest. Existing selective and well-characterized kinase inhibitors may be exploited to develop novel small molecule probes useful ...

journal_title：Molecular bioSystems

authors： Zhang Z,Kwiatkowski N,Zeng H,Lim SM,Gray NS,Zhang W,Yang PL

更新日期：2012-10-01 00:00:00

abstract：:Drug combinations have been widely applied to treat complex diseases, like cancer, HIV and cardiovascular diseases. One of the most important characteristics for drug combinations is the synergistic effects among different drugs, that is to say, the combination effects are larger than the sum of individual effects. Al...

journal_title：Molecular bioSystems

authors： Chen D,Zhang H,Lu P,Liu X,Cao H

更新日期：2016-02-01 00:00:00

abstract：:A growing number of diverse proteins are being identified that moonlight. Moonlighting proteins comprise an interesting subset of multifunctional proteins in which the two functions are found in a single polypeptide chain. They do not include proteins that are multifunctional due to gene fusions, families of homologou...

journal_title：Molecular bioSystems

authors： Jeffery CJ

更新日期：2009-04-01 00:00:00

abstract：:Cycloserine (CS, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (PLP)-dependent enzymes. Two CS enantiomers are known; D-cycloserine (DCS, also known as Seromycin) is a natural product that is used to treat resistant Mycobacterium tuberculosis infec...

journal_title：Molecular bioSystems

authors： Lowther J,Yard BA,Johnson KA,Carter LG,Bhat VT,Raman MC,Clarke DJ,Ramakers B,McMahon SA,Naismith JH,Campopiano DJ

更新日期：2010-09-01 00:00:00

abstract：:Candida glabrata has been recognized as an efficient industrial microorganism for the production of pyruvate, the most widely used α-oxocarboxylic acid in the areas of agrochemicals, drugs, and chemicals. To gain a comprehensive understanding of its physiology and cellular metabolism, the genome-scale metabolic model ...

journal_title：Molecular bioSystems

authors： Xu N,Liu L,Zou W,Liu J,Hua Q,Chen J

更新日期：2013-02-02 00:00:00

abstract：:The selective inhibition for JAK2 over the other JAK family kinases (JAK1, JAK3 and TYK2) has shared an immense challenge due to high conservatism. In this paper, the highly JAK2 selective mechanism of the thienopyridine derivative was identified at the molecular level, based on insights into the inhibitory effects of...

journal_title：Molecular bioSystems

authors： Li JJ,Cheng P,Tu J,Zhai HL,Zhang XY

更新日期：2016-02-01 00:00:00

abstract：:Hypoxia and inflammation have been identified as the hallmarks of colitis, intertwined with metabolism. Here, we report that halofuginone (HF), an antiparasitic drug, attenuates dextran sulfate sodium (DSS)-induced colitis in mice, as represented by attenuating the disease activity index, inhibiting colonic shortening...

journal_title：Molecular bioSystems

authors： Liu J,Xiao HT,Wang HS,Mu HX,Zhao L,Du J,Yang D,Wang D,Bian ZX,Lin SH

更新日期：2016-06-21 00:00:00

abstract：:Experimental data from biological pathways come in many forms: qualitative or quantitative, static or dynamic. By combining a variety of these heterogeneous sources of data, we construct a mathematical model of a critical regulatory network in vertebrate development, the Sonic Hedgehog signaling pathway. The structure...

journal_title：Molecular bioSystems

authors： Boykin ER,Ogle WO

更新日期：2010-10-01 00:00:00

abstract：:High levels of DNA and RNA released by apoptotic and necrotic cells circulate in the blood of cancer patients. In the present study we determined the applicability of the quantification of nucleic acids and their genetic alterations as minimally invasive tool for breast cancer screening. The relative concentrations of...

journal_title：Molecular bioSystems

authors： Schwarzenbach H,Müller V,Milde-Langosch K,Steinbach B,Pantel K

更新日期：2011-10-01 00:00:00

abstract：:The emergence of drug resistant strains of Mycobacterium Tuberculosis (Mtb) accentuates the urgent need for the development of novel antitubercular drugs. The major causes of drug resistance are genetic mutations, the influx-efflux transporter system, and the complex cell wall system of Mtb, which can function as perm...

journal_title：Molecular bioSystems

authors： Janardhan S,Ram Vivek M,Narahari Sastry G

更新日期：2016-10-18 00:00:00

abstract：:Lamellipodin (Lpd) protein plays an important role in the formation of lamellipodial protrusion which is crucial in actin dynamics, cell polarity and motility. Lpd promotes actin polymerization with the help of members of the Ena/VASP family of actin regulators and tethering them to actin filaments. It is well documen...

journal_title：Molecular bioSystems

authors： Gorai S,Paul D,Haloi N,Borah R,Santra MK,Manna D

更新日期：2016-03-01 00:00:00

abstract：:The emerging field of synthetic biology is generating insatiable demands for synthetic genes, which far exceed existing gene synthesis capabilities. This review discusses the current methods of chemical DNA synthesis and gene assembly, as well as the latest engineering tools, technologies and trends which could potent...

journal_title：Molecular bioSystems

authors： Tian J,Ma K,Saaem I

更新日期：2009-07-01 00:00:00

abstract：:Undoubtedly, metabonomics can reveal the comprehensive efficacies of traditional Chinese medicine (TCM) formulae and its complex mechanism at the molecular biological level. In this study, an attempt was made to address the pretreatment effect of a TCM formula. In this case, as a critical point, we should first know h...

journal_title：Molecular bioSystems

authors： Yan B,Deng Y,Hou J,Bi Q,Yang M,Jiang B,Liu X,Wu W,Guo D

更新日期：2015-02-01 00:00:00

abstract：:Secretagogin is a hexa EF-hand Ca(2+)-binding protein expressed in neuroendocrine, pancreatic endocrine and retinal cells. The protein has been noted for its expression in specific neuronal subtypes in the support of hierarchical organizing principles in the mammalian brain. Secretagogin has previously been found to i...

journal_title：Molecular bioSystems

authors： Bauer MC,O'Connell DJ,Maj M,Wagner L,Cahill DJ,Linse S

更新日期：2011-07-01 00:00:00

abstract：:Celastrol, isolated from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f. (Thunder God's Vine), has been used to treat cancer, chronic inflammatory, autoimmune and other human diseases. However, to date, the protein targets and the mechanism of action of celastrol have remained elusive. In this s...

journal_title：Molecular bioSystems

authors： Zhou Y,Li W,Wang M,Zhang X,Zhang H,Tong X,Xiao Y

更新日期：2016-12-20 00:00:00

abstract：:We recently reported a chemical genetic method for generating bivalent inhibitors of protein kinases. This method relies on the use of the DNA repair enzyme O(6)-alkylguanine-DNA alkyltransferase (AGT) to display an ATP-competitive inhibitor and a ligand that targets a secondary binding domain. With this method potent...

journal_title：Molecular bioSystems

authors： Hill ZB,Perera BG,Maly DJ

更新日期：2011-02-01 00:00:00

abstract：:Metabolomics is a rapidly growing branch of post-genomic chemical biology. The fission yeast Schizosaccharomyces pombe is an excellent eukaryotic model organism. Although the entire S. pombe genome has been sequenced and detailed transcriptomic analyses were performed, little metabolic profiling has been done. Here we...

journal_title：Molecular bioSystems

authors： Pluskal T,Nakamura T,Villar-Briones A,Yanagida M

更新日期：2010-01-01 00:00:00

abstract：:MicroiRNAs are genome encoded small double stranded RNAs that regulate expression of homologous mRNAs. With approximately 2500 human miRNAs and each having hundreds of potential mRNA targets, miRNA based gene regulation is quite pervasive in both development and disease. While there are numerous studies investigating ...

journal_title：Molecular bioSystems

authors： Chaulk SG,Ebhardt HA,Fahlman RP

更新日期：2016-01-01 00:00:00

abstract：:Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...

journal_title：Molecular bioSystems

authors： Zhang H,Zheng H,Zhao G,Tang C,Lu S,Cheng B,Wu F,Wei J,Liang Y,Ruan J,Song H,Su Z

更新日期：2016-03-01 00:00:00

abstract：:There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in ger...

journal_title：Molecular bioSystems

authors： Rejzek M,Stevenson CE,Southard AM,Stanley D,Denyer K,Smith AM,Naldrett MJ,Lawson DM,Field RA

更新日期：2011-03-01 00:00:00

abstract：:Human mitogen-activated protein kinase (MAPK) family members JNK and p38 are two homologous protein-serine/threonine kinases but play distinct roles in the pathological process of neurological disorders. Selective targeting of JNK over p38 has been established as a potential therapeutic approach to epilepsy and other ...

journal_title：Molecular bioSystems

authors： Zhuo ZH,Sun YZ,Jin PN,Li FY,Zhang YL,Wang HL

更新日期：2016-07-19 00:00:00

abstract：:Bacterial resistance to β-lactam antibiotics poses a serious threat to human health. Penicillin binding proteins (PBPs) and β-lactamases are involved in both antibacterial activity and mediation of β-lactam antibiotic resistance. The two major reasons for resistance to β-lactams include: (i) pathogenic bacteria expres...

journal_title：Molecular bioSystems

authors： Kumar KM,Anbarasu A,Ramaiah S

更新日期：2014-04-01 00:00:00

abstract：:Sequence specific binding of DNA and RNA is of fundamental importance in the regulation of cellular gene expression. Because of their modular structure repeat domain proteins are particularly well suited for these processes and have been widely adopted throughout evolution. Detailed biochemical and structural data has...

journal_title：Molecular bioSystems

authors： Filipovska A,Rackham O

更新日期：2012-03-01 00:00:00

abstract：:To identify an agent with specific activity against human lens epithelial cells (HLECs), we confirmed the presence of glucocorticoid receptors (GRs) and GR-α genes and evaluated whether GRs have a relationship with the apoptotic process in cultured HLECs. We also determined whether the inhibitor RU486 could rescue the...

journal_title：Molecular bioSystems

authors： Wang L,Zhao W,Leng F,Ge J,Bu Z,Zhang Y,Liu P

更新日期：2011-06-01 00:00:00

abstract：:A lipase substrate microarray was obtained by printing aliphatic C2-C12 monoesters of (5R)- and (5S)-3-(5,6-dihydroxyhexyloxy)benzaldehyde by reductive alkylation on amine-functionalized glass slides coated with bovine serum albumin and a short PEG linker. The microarray features 12 substrates and their 66 possible bi...

journal_title：Molecular bioSystems

authors： Grognux J,Reymond JL

更新日期：2006-10-01 00:00:00

abstract：:Mirror image phage display is a straightforward approach to identify new potentially therapeutically active D-enantiomeric peptides. Such D-peptides are more resistant to proteolytic degradation compared to L-peptides. In this review, several examples of mirror image phage display derived D-peptides with therapeutical...

journal_title：Molecular bioSystems

authors： Funke SA,Willbold D

更新日期：2009-08-01 00:00:00

abstract：:Biological networks are effective tools for studying molecular interactions. Modular structure, in which genes or proteins may tend to be associated with functional modules or protein complexes, is a remarkable feature of biological networks. Mining modular structure from biological networks enables us to focus on a s...

journal_title：Molecular bioSystems

authors： Jiao QJ,Huang Y,Shen HB

更新日期：2016-11-15 00:00:00