Abstract:
:A lipase substrate microarray was obtained by printing aliphatic C2-C12 monoesters of (5R)- and (5S)-3-(5,6-dihydroxyhexyloxy)benzaldehyde by reductive alkylation on amine-functionalized glass slides coated with bovine serum albumin and a short PEG linker. The microarray features 12 substrates and their 66 possible binary mixtures spotted in a 9 x 36 spot array. Lipase reactions are detected by chemoselective NaIO(4)-oxidation of the 1,2-diol hydrolysis product to form an aldehyde, which is then tagged with the red-fluorescent dye rhodamine B sulfohydrazide . Specific fingerprints are produced by active enzymes. These experiments provide the first example of lipase fingerprinting using microarrays.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Grognux J,Reymond JLdoi
10.1039/b609275fsubject
Has Abstractpub_date
2006-10-01 00:00:00pages
492-8issue
10eissn
1742-206Xissn
1742-2051journal_volume
2pub_type
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