N-Succinyl-chitosan grafted with low molecular weight polyethylenimine as a serum-resistant gene vector.

abstract：:The small molecule 4-hydroxy-3-methoxybenzaldehyde (5,6,7,8-tetrahydro[1]benzothieno[2,3-d]pyrimidin-4-yl)hydrazone (Exo2) stimulates morphological changes at the mammalian Golgi and trans-Golgi network that are virtually indistinguishable from those induced by brefeldin A. Both brefeldin A and Exo2 protect cells from...

journal_title：Molecular bioSystems

authors： Guetzoyan LJ,Spooner RA,Boal F,Stephens DJ,Lord JM,Roberts LM,Clarkson GJ

更新日期：2010-10-01 00:00:00

abstract：:The use of Cyan Fluorescent Proteins, with a distinctive lifetime signature, opens up new alternatives to track and semi-quantify the relative expression of proteins in vivo using a single excitation source and emission channel. ...

journal_title：Molecular bioSystems

authors： Kim J,Kwon D,Lee J,Pasquier H,Grailhe R

更新日期：2009-02-01 00:00:00

abstract：:There are clearly many different philosophies associated with adapting fragment screening into mainstream Drug Discovery Lead Generation strategies. Scientists at Astex, for instance, focus entirely on strategies involving use of X-ray crystallography and NMR. However, AstraZeneca uses a number of different fragment s...

journal_title：Molecular bioSystems

authors： Leach AR,Hann MM,Burrows JN,Griffen EJ

更新日期：2006-09-01 00:00:00

abstract：:Establishing functional relationships between multi-domain protein sequences is a non-trivial task. Traditionally, delineating functional assignment and relationships of proteins requires domain assignments as a prerequisite. This process is sensitive to alignment quality and domain definitions. In multi-domain protei...

journal_title：Molecular bioSystems

authors： Bhaskara RM,Mehrotra P,Rakshambikai R,Gnanavel M,Martin J,Srinivasan N

更新日期：2014-05-01 00:00:00

abstract：:The non-classical zinc finger protein, Neural Zinc Finger Factor-1, contains six Cys2His2Cys domains. All three cysteines and the second histidine directly bind Zn(II). Using a combination of mutagenesis, metal coordination and DNA binding studies, we report that the first histidine is involved in a functionally impor...

journal_title：Molecular bioSystems

authors： Besold AN,Amick DL,Michel SL

更新日期：2014-07-01 00:00:00

abstract：:As a potential target for the treatment of schizophrenia, the dual cAMP/cGMP hydrolyzing enzyme PDE10A has attracted a significant amount of attention. In the present work, the inhibition mechanism of 116 structurally diverse quinoline derivatives as PDE10A inhibitors was explored by 3D-QSAR, molecular docking and mol...

journal_title：Molecular bioSystems

authors： Wu Q,Gao Q,Guo H,Li D,Wang J,Gao W,Han C,Li Y,Yang L

更新日期：2013-03-01 00:00:00

abstract：:The interaction of spermine and its analogue, 1-naphthyl acetyl spermine with four double stranded DNA polynucleotides has been studied to understand the structural and thermodynamic basis of the binding. The efficacy and specificity of DNA binding of this analogue has not yet been revealed. The energetics of the inte...

journal_title：Molecular bioSystems

authors： Kabir A,Kumar GS

更新日期：2014-05-01 00:00:00

abstract：:Microbial long chain alcohols and alkanes are renewable biofuels that could one day replace petroleum-derived fuels. Here we report a novel pathway for high efficiency production of these products in Escherichia coli strain BL21(DE3). We first identified the acyl-ACP reductase/aldehyde deformylase combinations with th...

journal_title：Molecular bioSystems

authors： Coursolle D,Lian J,Shanklin J,Zhao H

更新日期：2015-09-01 00:00:00

abstract：:Dynamic physical interactions between proteins underpin all key cellular processes and are a highly attractive area for the development of research tools and medicines. Protein-protein interactions frequently involve α-helical structures, but peptides matching the sequences of these structures usually do not fold corr...

journal_title：Molecular bioSystems

authors： Mart RJ,Errington RJ,Watkins CL,Chappell SC,Wiltshire M,Jones AT,Smith PJ,Allemann RK

更新日期：2013-11-01 00:00:00

abstract：:Undoubtedly, metabonomics can reveal the comprehensive efficacies of traditional Chinese medicine (TCM) formulae and its complex mechanism at the molecular biological level. In this study, an attempt was made to address the pretreatment effect of a TCM formula. In this case, as a critical point, we should first know h...

journal_title：Molecular bioSystems

authors： Yan B,Deng Y,Hou J,Bi Q,Yang M,Jiang B,Liu X,Wu W,Guo D

更新日期：2015-02-01 00:00:00

abstract：:The significant increase in the demand for purified protein for crystallization and structural studies has made necessary the development of multi-sample methods for identifying solution conditions that affect protein stability and aggregation. Conditions that stabilize proteins can improve protein purification and cr...

journal_title：Molecular bioSystems

authors： Senisterra GA,Finerty PJ Jr

更新日期：2009-03-01 00:00:00

abstract：:Celastrol, isolated from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f. (Thunder God's Vine), has been used to treat cancer, chronic inflammatory, autoimmune and other human diseases. However, to date, the protein targets and the mechanism of action of celastrol have remained elusive. In this s...

journal_title：Molecular bioSystems

authors： Zhou Y,Li W,Wang M,Zhang X,Zhang H,Tong X,Xiao Y

更新日期：2016-12-20 00:00:00

abstract：:Remineralization of organic matter in deep-sea sediments is important in oceanic biogeochemical cycles, and bacteria play a major role in this process. Shewanella violacea DSS12 is a psychrophilic and piezophilic gamma-proteobacterium that was isolated from the surface layer of deep sea sediment at a depth of 5110 m. ...

journal_title：Molecular bioSystems

authors： Aono E,Baba T,Ara T,Nishi T,Nakamichi T,Inamoto E,Toyonaga H,Hasegawa M,Takai Y,Okumura Y,Baba M,Tomita M,Kato C,Oshima T,Nakasone K,Mori H

更新日期：2010-07-01 00:00:00

abstract：:The mono-site mutations of the absolutely conserved residues, (464)LGR(466), in the α-helix 5 (h5) of HPV16 L1 completely disrupted the pentamer formation. The implication of this finding is the potential usage of a h5-like peptide as the reagent to interfere with the pentamer formation and stability as an anti-HPV re...

journal_title：Molecular bioSystems

authors： Jin S,Pan D,Zha X,Yu X,Wu Y,Liu Y,Yin F,Chen XS

更新日期：2014-04-01 00:00:00

abstract：:The interaction of Phospholipase D1 (PLD1) by its C-terminal domain D4 with PED/PEA15 has been indicated as a target for type 2 diabetes. PED/PEA15 is overexpressed in several tissues of individuals affected by type 2 diabetes and its overexpression in intact cells and in transgenic animal models impairs insulin regul...

journal_title：Molecular bioSystems

authors： Doti N,Cassese A,Marasco D,Paturzo F,Sabatella M,Viparelli F,Dathan N,Monti SM,Miele C,Formisano P,Beguinot F,Ruvo M

更新日期：2010-10-01 00:00:00

abstract：:TGF-β1, 2 and 3 cytokines are involved in many cellular processes including cell proliferation, differentiation, migration and survival. Whereas TGF-β2 and 3 play important roles in embryonic development, TGF-β1 is mostly implicated in controlling immune responses after birth. The production of TGF-β1 is a tightly reg...

journal_title：Molecular bioSystems

authors： Stockis J,Dedobbeleer O,Lucas S

更新日期：2017-09-26 00:00:00

abstract：:Cystic fibrosis (CF) is an autosomal recessive disorder associated with mutations of the cystic fibrosis transmembrane conductance regulator (CFTR) gene and defective chloride transport across the epithelial cell membranes. Abnormal epithelial ion transport is the primary cause of persistent airway infections and chro...

journal_title：Molecular bioSystems

authors： Ciavardelli D,D'Orazio M,Pieroni L,Consalvo A,Rossi C,Sacchetta P,Di Ilio C,Battistoni A,Urbani A

更新日期：2013-06-01 00:00:00

abstract：:Protein phosphorylation is associated with most cell signaling and developmental processes in eukaryotes. Despite the vast extent of the phosphoproteome within the cell, connecting specific kinases with relevant targets remains a significant experimental frontier. The challenge of linking kinases and their substrates ...

journal_title：Molecular bioSystems

authors： Sopko R,Andrews BJ

更新日期：2008-09-01 00:00:00

abstract：:To faithfully execute diverse biological programs all cells need to access and distribute their genomes in a highly organized way. In the nucleus of eukaryotic cells DNA is packed with histone proteins into chromatin. The originating nucleo-protein complex is the regulatory platform for all genetic processes. Of these...

journal_title：Molecular bioSystems

authors： Nikolov M,Fischle W

更新日期：2013-02-02 00:00:00

abstract：:When exposed to low concentrations of toxic chemicals, bacteria modulate the expression of a number of cellular processes. Typically, these processes include those related to porin production, dismutases, and metabolic fluxes. In Escherichia coli (E. coli), the expression of these systems is largely controlled by thre...

journal_title：Molecular bioSystems

authors： Jain K,Saini S

更新日期：2016-05-24 00:00:00

abstract：:The emergence of drug resistant strains of Mycobacterium Tuberculosis (Mtb) accentuates the urgent need for the development of novel antitubercular drugs. The major causes of drug resistance are genetic mutations, the influx-efflux transporter system, and the complex cell wall system of Mtb, which can function as perm...

journal_title：Molecular bioSystems

authors： Janardhan S,Ram Vivek M,Narahari Sastry G

更新日期：2016-10-18 00:00:00

abstract：:A cyclic dinucleotide riboswitch has been fused with a G-quadruplex motif to produce a conditional riboswitch-peroxidase-mimicking sensor that oxidizes both colorimetric and fluorogenic substrates in the presence of c-di-GMP. We find that signal-to-noise ratio could be improved by using a two-, not three-, floor split...

journal_title：Molecular bioSystems

authors： Tsuji G,Sintim HO

更新日期：2016-03-01 00:00:00

abstract：:In this review we bring together recent results from our group focused towards the development of biosensors from single conically-shaped artificial nanopores. The nanopores, used in the work presented here, were prepared using the track-etch process. The fabrication of track-etched conical nanopores has been optimize...

journal_title：Molecular bioSystems

authors： Sexton LT,Horne LP,Martin CR

更新日期：2007-10-01 00:00:00

abstract：:Conversion of the predominantly α-helical cellular prion protein (PrP(C)) to the misfolded β-sheet enriched Scrapie form (PrP(Sc)) is a critical event in prion pathogenesis. However, the conformational triggers that lead to the isoform conversion (PrP(C) to PrP(Sc)) remain obscure, and conjectures about the role of un...

journal_title：Molecular bioSystems

authors： Menon S,Sengupta N

更新日期：2015-05-01 00:00:00

abstract：:In the last few decades a negative association between the level of milk production and fertility has been observed. Currently, the most utilized method of measuring male fertility employed by the livestock industry is related to the Non-Return Rate (NRR). Through differential proteome analysis, this study evaluated c...

journal_title：Molecular bioSystems

authors： Soggiu A,Piras C,Hussein HA,De Canio M,Gaviraghi A,Galli A,Urbani A,Bonizzi L,Roncada P

更新日期：2013-06-01 00:00:00

abstract：:Thyroid carcinoma is a common endocrine malignancy worldwide, accounting for approximately 1% of all diagnosed cancers and about 91.5% of the malignancies of head and neck. However, differentiating malignant thyroid nodules from benign ones remains a diagnostic challenge. Thus, novel molecular markers that enable non-...

journal_title：Molecular bioSystems

authors： Yao Z,Yin P,Su D,Peng Z,Zhou L,Ma L,Guo W,Ma L,Xu G,Shi J,Jiao B

更新日期：2011-09-01 00:00:00

abstract：:G protein-coupled receptors (GPCRs) are key signaling proteins that regulate how cells interact with their environment. Traditional signaling cascades involving GPCRs have been well described and are well established and very important clinical targets. With the development of more recent technologies, hints about the...

journal_title：Molecular bioSystems

authors： Zhang X,Eggert US

更新日期：2013-04-05 00:00:00

abstract：:Here, we used stopped-flow fluorescence techniques to conduct a comparative kinetic analysis of the conformational transitions in human apurinic/apyrimidinic endonuclease 1 (APE1) and in DNA containing an abasic site in the course of their interaction. Effects of monovalent (K(+)) and divalent (Mg(2+), Mn(2+), Ca(2+),...

journal_title：Molecular bioSystems

authors： Miroshnikova AD,Kuznetsova AA,Vorobjev YN,Kuznetsov NA,Fedorova OS

更新日期：2016-05-26 00:00:00

abstract：:The selective inhibition for JAK2 over the other JAK family kinases (JAK1, JAK3 and TYK2) has shared an immense challenge due to high conservatism. In this paper, the highly JAK2 selective mechanism of the thienopyridine derivative was identified at the molecular level, based on insights into the inhibitory effects of...

journal_title：Molecular bioSystems

authors： Li JJ,Cheng P,Tu J,Zhai HL,Zhang XY

更新日期：2016-02-01 00:00:00

abstract：:Epithelial ovarian cancer (EOC) is the most lethal of gynecologic malignancies due to the high rate of recurrence and poor prognosis. Predicting the prognosis in patients with EOC is clinically challenging, partly because appropriate biomarkers of recurrence have yet to be explored. In this prospective study, pre-trea...

journal_title：Molecular bioSystems

authors： Zhang H,Ge T,Cui X,Hou Y,Ke C,Yang M,Yang K,Wang J,Guo B,Zhang F,Lou G,Li K

更新日期：2015-02-01 00:00:00