Abstract:
:A cyclic dinucleotide riboswitch has been fused with a G-quadruplex motif to produce a conditional riboswitch-peroxidase-mimicking sensor that oxidizes both colorimetric and fluorogenic substrates in the presence of c-di-GMP. We find that signal-to-noise ratio could be improved by using a two-, not three-, floor split G-quadruplex for this conditional peroxidase-mimicking riboswitch.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Tsuji G,Sintim HOdoi
10.1039/c5mb00751hsubject
Has Abstractpub_date
2016-03-01 00:00:00pages
773-7issue
3eissn
1742-206Xissn
1742-2051journal_volume
12pub_type
杂志文章abstract::In actively proliferating cells, such as the cells of the developing embryo, DNA repair is crucial for preventing the accumulation of mutations and synchronizing cell division. Sea urchin embryo growth was analyzed and extracts were prepared. The relative activity of DNA polymerase, apurinic/apyrimidinic (AP) endonucl...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00906e
更新日期:2016-06-21 00:00:00
abstract::Unbalanced uptake of Omega 6/Omega 3 (ω-6/ω-3) ratios could increase chronic disease occurrences, such as inflammation, atherosclerosis, or tumor proliferation, and methylation methods for measuring the ruminal microbiome fatty acid (FA) composition/distribution play a vital role in discovering the contribution of foo...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00325c
更新日期:2015-11-01 00:00:00
abstract::We recently reported a chemical genetic method for generating bivalent inhibitors of protein kinases. This method relies on the use of the DNA repair enzyme O(6)-alkylguanine-DNA alkyltransferase (AGT) to display an ATP-competitive inhibitor and a ligand that targets a secondary binding domain. With this method potent...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00108b
更新日期:2011-02-01 00:00:00
abstract::Intrinsically disordered regions in proteins are known to evolve rapidly while maintaining their function. However, given their lack of structure and sequence conservation, the means through which they stay functional is not clear. Poor sequence conservation also hampers the classification of these regions into functi...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25202c
更新日期:2012-10-30 00:00:00
abstract::The identification of a suitable non-fluorescent molecule that can be oxidized by G-quadruplexes into a fluorescent product will be important for several fields such as bioanalyte sensing and cancer therapeutic discovery. Herein, we demonstrate that 2',7'-dichlorodihydrofluorescein diacetate is a superior reducing sub...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b916228c
更新日期:2010-01-01 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) is increased worldwide in parallel with the obesity epidemic. Potentilla discolor is one of the most important crude materials in Traditional Chinese medicine (TCM) for therapy of hyperglycemia and hyperlipidemia. In this work, a plasma metabonomic approach based on the combination of U...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00278d
更新日期:2014-11-01 00:00:00
abstract::RNA-binding proteins (RBPs) are key regulators of gene expression. Some long non-coding RNAs (lncRNAs) affect gene expression by interacting with RBPs. However, whether this influences the biological characteristics of lncRNAs in diseases still remains unknown. Here, we classify lncRNAs into two categories, using the ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00144d
更新日期:2017-06-01 00:00:00
abstract::Nicotinamide adenine dinucleotide (NAD) is a ubiquitous cofactor participating in numerous redox reactions. It is also a substrate for regulatory modifications of proteins and nucleic acids via the addition of ADP-ribose moieties or removal of acyl groups by transfer to ADP-ribose. In this study, we use in-depth seque...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb05487f
更新日期:2012-06-01 00:00:00
abstract::Non-ribosomal peptide synthetases (NRPSs) are enzymes that catalyze ribosome-independent production of small peptides, most of which are bioactive. NRPSs act as peptide assembly lines where individual, often interconnected modules each incorporate a specific amino acid into the nascent chain. The modules themselves co...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70594c
更新日期:2014-07-01 00:00:00
abstract::The molecular segmentation clock is a complex regulatory network that governs the periodic somite segmentation in vertebrate embryos. Underlying the rhythm of the segmentation clock is a single-cell level pace-making circuit, where inevitable molecular noise and time delay impose normal operating constraints to the pa...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70046a
更新日期:2013-06-01 00:00:00
abstract::Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP-proflavine-hemin nucleotidezyme can oxidize the colorless compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS˙(+) ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb05430b
更新日期:2012-03-01 00:00:00
abstract::Plant aquaporins belong to a large superfamily of conserved proteins called the major intrinsic proteins (MIPs). There is limited information about the diversity of MIPs and their water transport capacity in broccoli (Brassica oleracea) plants. In this study, the cDNAs of isoforms of Plasma Membrane Intrinsic Proteins...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00285b
更新日期:2011-04-01 00:00:00
abstract::Upon rapid ascent to a high altitude, non-acclimatized individuals, although healthy, are highly prone to contracting high-altitude pulmonary edema (HAPE). Early diagnosis is difficult and there is no reliable biomarker available. We used proton ((1)H) NMR metabolomics to profile the altered metabolic patterns of bloo...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25044f
更新日期:2012-06-01 00:00:00
abstract::Blockage of the interactions between immunologic checkpoint protein PD-1 and its ligand PD-L1 showed efficacy for cancer treatment. X-ray structures have captured static conformational snapshots of PD-1 and revealed that the CC' loop adopts an open conformation in the apo-protein but turns into a closed form and inter...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00036g
更新日期:2017-05-02 00:00:00
abstract::The emergence of drug resistant strains of Mycobacterium Tuberculosis (Mtb) accentuates the urgent need for the development of novel antitubercular drugs. The major causes of drug resistance are genetic mutations, the influx-efflux transporter system, and the complex cell wall system of Mtb, which can function as perm...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00457a
更新日期:2016-10-18 00:00:00
abstract::The adaptation of the cellular network to functional changes, timing and patterning of gene expression is regulated by binding of transcription factors to gene regulatory elements, which in turn depends on co-occurring histone modifications. These two layers influence each other, enabling a further level of regulatory...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05363a
更新日期:2012-02-01 00:00:00
abstract::To faithfully execute diverse biological programs all cells need to access and distribute their genomes in a highly organized way. In the nucleus of eukaryotic cells DNA is packed with histone proteins into chromatin. The originating nucleo-protein complex is the regulatory platform for all genetic processes. Of these...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c2mb25328c
更新日期:2013-02-02 00:00:00
abstract::In the post-genome era, one of the most important and challenging tasks is to identify the subcellular localizations of protein complexes, and further elucidate their functions in human health with applications to understand disease mechanisms, diagnosis and therapy. Although various experimental approaches have been ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25451h
更新日期:2013-04-05 00:00:00
abstract::In this review we bring together recent results from our group focused towards the development of biosensors from single conically-shaped artificial nanopores. The nanopores, used in the work presented here, were prepared using the track-etch process. The fabrication of track-etched conical nanopores has been optimize...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b708725j
更新日期:2007-10-01 00:00:00
abstract::The effect of aging and development in male Wistar-derived rats on the profile of endogenous metabolites excreted in the urine was investigated using both (1)H NMR spectroscopy and HPLC-TOF MS using electrospray ionisation (ESI). The endogenous metabolites were profiled in samples collected from male rats every two we...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b500852b
更新日期:2005-07-01 00:00:00
abstract::The dairy bacterium Lactococcus lactis has to master a complicated task. It must control its essentially linear glycolytic pathway in such a fashion that, when the substrate, glucose, runs out, it retains enough phosphoenolpyruvate and fructose-1,6-bisphosphate to be able to restart glycolysis as soon as new glucose b...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00726g
更新日期:2016-01-01 00:00:00
abstract::Recently, more and more research has shown that microRNAs (miRNAs) play critical roles in the development and progression of various diseases, but it is not easy to predict potential human miRNA-disease associations from the vast amount of biological data. Computational methods for predicting potential disease-miRNA a...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25180a
更新日期:2012-10-01 00:00:00
abstract::Protein tyrosine phosphatases (PTPs) constitute a large family of enzymes that play key roles in cell signaling. Deregulation of PTP activity results in aberrant tyrosine phosphorylation, which has been linked to the etiology of several human diseases, including cancer. Since phosphate removal by the PTPs can both enh...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b700704n
更新日期:2007-05-01 00:00:00
abstract::Genome sequencing technology has generated a vast amount of genomic and epigenomic data, and has provided us a great opportunity to study gene functions on a global scale from an epigenomic view. In the last decade, network-based studies, such as those based on PPI networks and co-expression networks, have shown good ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00430c
更新日期:2017-10-24 00:00:00
abstract::Over the past decade there has been a growing acknowledgement that a large proportion of proteins within most proteomes contain disordered regions. Disordered regions are segments of the protein chain which do not adopt a stable structure. Recognition of disordered regions in a protein is of great importance for prote...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c1mb05207a
更新日期:2012-01-01 00:00:00
abstract::Celastrol, isolated from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f. (Thunder God's Vine), has been used to treat cancer, chronic inflammatory, autoimmune and other human diseases. However, to date, the protein targets and the mechanism of action of celastrol have remained elusive. In this s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00691d
更新日期:2016-12-20 00:00:00
abstract::The identification of disulfide bonds provides critical information regarding the structure and function of a protein and is a key aspect in understanding signaling cascades in biological systems. Recent proteomic approaches using digestion enzymes have facilitated the characterization of disulfide-bonds and/or oxidiz...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00688g
更新日期:2015-04-01 00:00:00
abstract::To understand the regulatory dynamics of transcription factors (TFs) and their interplay with other cellular components we have integrated transcriptional, protein-protein and the allosteric or equivalent interactions which mediate the physiological activity of TFs in Escherichia coli. To study this integrated network...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25279a
更新日期:2012-11-01 00:00:00
abstract::Electrospray ionization mass spectrometry combined with high efficiency capillary liquid chromatography provides high sensitivity and broad dynamic range measurements for the characterization of biological macromolecules in complex matrices, and is an increasingly powerful analytical tool for systems biology research....
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b601468b
更新日期:2006-05-01 00:00:00
abstract::MicroiRNAs are genome encoded small double stranded RNAs that regulate expression of homologous mRNAs. With approximately 2500 human miRNAs and each having hundreds of potential mRNA targets, miRNA based gene regulation is quite pervasive in both development and disease. While there are numerous studies investigating ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00415b
更新日期:2016-01-01 00:00:00