Abstract:
:Herein, we demonstrate that the bacterial signaling molecule, c-di-GMP, can enhance the peroxidation of hemin when proflavine is present. The c-di-GMP-proflavine-hemin nucleotidezyme can oxidize the colorless compound 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), ABTS, to the colored radical cation ABTS˙(+) and hence provides simple colorimetric detection of c-di-GMP at low micromolar concentrations.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Nakayama S,Roelofs K,Lee VT,Sintim HOdoi
10.1039/c2mb05430bsubject
Has Abstractpub_date
2012-03-01 00:00:00pages
726-9issue
3eissn
1742-206Xissn
1742-2051journal_volume
8pub_type
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