BH3 helix-derived biophotonic nanoswitches regulate cytochrome c release in permeabilised cells.

Abstract:

:Dynamic physical interactions between proteins underpin all key cellular processes and are a highly attractive area for the development of research tools and medicines. Protein-protein interactions frequently involve α-helical structures, but peptides matching the sequences of these structures usually do not fold correctly in isolation. Therefore, much research has focused on the creation of small peptides that adopt stable α-helical structures even in the absence of their intended protein targets. We show that short peptides alkylated with azobenzene crosslinkers can be used to photo-stimulate mitochondrial membrane depolarization and cytochrome c release in permeabilised cells, the initial events of the intrinsic apoptosis pathway.

journal_name

Mol Biosyst

journal_title

Molecular bioSystems

authors

Mart RJ,Errington RJ,Watkins CL,Chappell SC,Wiltshire M,Jones AT,Smith PJ,Allemann RK

doi

10.1039/c3mb70246d

subject

Has Abstract

pub_date

2013-11-01 00:00:00

pages

2597-603

issue

11

eissn

1742-206X

issn

1742-2051

journal_volume

9

pub_type

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