Abstract:
:Type 2 diabetes mellitus (T2DM) is increased worldwide in parallel with the obesity epidemic. Potentilla discolor is one of the most important crude materials in Traditional Chinese medicine (TCM) for therapy of hyperglycemia and hyperlipidemia. In this work, a plasma metabonomic approach based on the combination of UPLC-Q-TOF with multivariate data analysis was applied to investigate the therapeutic effects of the extract of P. discolor (EPD) and corosolic acid (CA), the main bioactive compounds of P. discolor. Male C57BL/6 mice were fed with high-fat diet (HFD-fed group) for 8 weeks and then treated with EPD (EPD-treated group) or CA (CA-treated group) for another 8 weeks. After the experimental period, samples of plasma were collected and analyzed by ultra-performance liquid chromatography/quadrupole time of flight mass spectrometry (UPLC-Q-TOF). The principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) models were built to find biomarkers of T2DM and investigate the therapeutic effects of EPD and CA. 26 metabolites, which are distributed in several metabolic pathways, were identified as potential biomarkers of T2DM. It was found that EPD and CA could reverse the pathological process of T2DM through regulating the disturbed pathway of metabolism. The metabonomic results are beneficial not only for the evaluation of the therapeutic effect of TCM but also for the elucidation of the underlying molecular mechanism.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Li Y,Li JJ,Wen XD,Pan R,He YS,Yang Jdoi
10.1039/c4mb00278dsubject
Has Abstractpub_date
2014-11-01 00:00:00pages
2898-906issue
11eissn
1742-206Xissn
1742-2051journal_volume
10pub_type
杂志文章abstract::Histone deacetylases (HDACs) contribute to regulation of gene expression by mediating higher-order chromatin structures. They assemble into large multiprotein complexes that regulate activity and specificity. We report the development of small molecule probes with class IIa and pan-HDAC activity that contain photoreac...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00109b
更新日期:2016-05-24 00:00:00
abstract::Analysis of complex formation between amyloid-β fragments using surface plasmon resonance biosensing and electrospray mass spectrometry reveals that region 11-14 mediates zinc-induced dimerization of amyloid-β and may serve as a potential drug target for preventing development and progression of Alzheimer's disease. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00334d
更新日期:2011-04-01 00:00:00
abstract::Microtubules (mt) are highly dynamic polymers composed of alpha- and beta-tubulin monomers that are present in all dividing and non-dividing cells. A broad variety of natural products exists that are known to interfere with the microtubule network, by either stabilizing or de-stabilizing these rope-like polymers. Amon...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25144b
更新日期:2012-08-01 00:00:00
abstract::A cyclic dinucleotide riboswitch has been fused with a G-quadruplex motif to produce a conditional riboswitch-peroxidase-mimicking sensor that oxidizes both colorimetric and fluorogenic substrates in the presence of c-di-GMP. We find that signal-to-noise ratio could be improved by using a two-, not three-, floor split...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00751h
更新日期:2016-03-01 00:00:00
abstract::This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25470k
更新日期:2013-05-01 00:00:00
abstract::New-type oseltamivir-resistant H1N1 influenza viruses have been a major threat to human health since the 2009 flu pandemic. To resolve the drug resistance issue, we aimed to identify a new type of inhibitors against H1 from traditional Chinese medicine (TCM) by employing the world's largest TCM database () for virtual...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05320e
更新日期:2011-12-01 00:00:00
abstract::Breast cancer has been reported as one of the most frequently diagnosed malignant diseases and the leading cause of cancer death in women all around the world. Furthermore, this complicated cancer is divided into multiple subtypes which present different clinical symptoms and need correspondingly directed therapy. We ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00653a
更新日期:2017-05-02 00:00:00
abstract::TGF-β1, 2 and 3 cytokines are involved in many cellular processes including cell proliferation, differentiation, migration and survival. Whereas TGF-β2 and 3 play important roles in embryonic development, TGF-β1 is mostly implicated in controlling immune responses after birth. The production of TGF-β1 is a tightly reg...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c7mb00251c
更新日期:2017-09-26 00:00:00
abstract::Polyethylenimine (PEI, especially with M(w) of 25,000) has been known as an efficient gene carrier and a gold standard of gene transfection due to its high transfection efficiency (TE). However, high concomitant cytotoxicity limited the application of PEI. In this report, several cationic polymers derived from low mol...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00339e
更新日期:2011-04-01 00:00:00
abstract::Transcription factors (TFs) and microRNAs (miRNAs) can jointly regulate transcriptional networks in the form of recurrent circuits or motifs. A motif can be divided into a feedforward loop (FFL) and a feedback loop (FBL). Incoherent FFLs have been the recent focus due to their potential to dampen gene expression noise...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00526k
更新日期:2014-12-01 00:00:00
abstract::Cyclic adenosine monophosphate and cyclic guanosine monophosphate are universal intracellular messengers whose concentrations are regulated by molecular networks comprised of different isoforms of the synthases adenylate cyclase or guanylate cyclase and the phosphodiesterases which degrade these compounds. In this pap...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b807993e
更新日期:2009-01-01 00:00:00
abstract::The Parkinson's disease (PD) associated gene PINK1 encodes a protein kinase that mediates the phosphorylation of multiple proteins involved in mitochondrial homeostasis. The broader downstream signaling events mediated by PINK1 kinase activity have not been well documented. We combine quantitative phosphoproteomic str...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70565j
更新日期:2014-07-01 00:00:00
abstract::Diabetic nephropathy (DN) is a serious metabolic disease, and comprehensive understanding of its complex mechanism will help in preventing the onset and progression of DN. To reveal the systemic metabolic changes associated with renal injury, we performed 1H NMR-based metabonomic and multivariate analyses to analyze s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70212j
更新日期:2013-11-01 00:00:00
abstract::Blockage of the interactions between immunologic checkpoint protein PD-1 and its ligand PD-L1 showed efficacy for cancer treatment. X-ray structures have captured static conformational snapshots of PD-1 and revealed that the CC' loop adopts an open conformation in the apo-protein but turns into a closed form and inter...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00036g
更新日期:2017-05-02 00:00:00
abstract::G protein-coupled receptors (GPCRs) are key signaling proteins that regulate how cells interact with their environment. Traditional signaling cascades involving GPCRs have been well described and are well established and very important clinical targets. With the development of more recent technologies, hints about the...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c2mb25429h
更新日期:2013-04-05 00:00:00
abstract::The essential idea of regenerative medicine is to fix or replace tissues or organs with alive and patient-specific implants. Pluripotent stem cells are able to indefinitely self-renew and differentiate into all cell types of the body which makes them a potent substantial player in regenerative medicine. The easily acc...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c6mb00595k
更新日期:2017-01-31 00:00:00
abstract::Homogeneous oligodeoxyguanylates incorporating small-molecule intercalator pyrene moieties in a 1,5 relationship form various structures of dimer, trimer, tetramer and internal hairpin form that are yellowish in color because of the intermolecular interactions between the pyrene units. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b926677a
更新日期:2010-06-01 00:00:00
abstract::Cycloserine (CS, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (PLP)-dependent enzymes. Two CS enantiomers are known; D-cycloserine (DCS, also known as Seromycin) is a natural product that is used to treat resistant Mycobacterium tuberculosis infec...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c003743e
更新日期:2010-09-01 00:00:00
abstract::Renal cell carcinoma (RCC), the most common neoplasm affecting the adult kidney, is characterised by heterogeneity of histological subtypes, drug resistance, and absence of molecular markers. Two-dimensional difference gel electrophoresis (2-D DIGE) technology in combination with mass spectrometry (MS) was applied to ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb05390j
更新日期:2012-04-01 00:00:00
abstract::The neuroscientific community rapidly adopted RNA interference techniques as an experimental tool for the dissection of gene function in vitro and in animal models of neurological disease in vivo. Here, we discuss recent advances in the biotechnical implementation of siRNA/shRNA-mediated gene silencing focusing on iss...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b701169e
更新日期:2007-11-01 00:00:00
abstract::Epithelial to mesenchymal transition (EMT) is a biological process that plays a crucial role in cancer metastasis. Although studies regarding the EMT mechanisms are usual in terms of gene expression and protein functions, little is known about the involvement of lipids in EMT. In this work, an untargeted lipidomic ana...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00413f
更新日期:2015-12-01 00:00:00
abstract::A recent model proposing that a barrier is raised against tumor evolution in pre-cancer tissues is investigated. For that we quantify expression alterations in genome maintenance pathways: DNA damage response, death pathways and cell cycle and also differentially expressed genes in transcriptomes of pre-cancerous and ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25242b
更新日期:2012-11-01 00:00:00
abstract::The significant increase in the demand for purified protein for crystallization and structural studies has made necessary the development of multi-sample methods for identifying solution conditions that affect protein stability and aggregation. Conditions that stabilize proteins can improve protein purification and cr...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b814377c
更新日期:2009-03-01 00:00:00
abstract::Mass spectrometry (MS) has become an increasingly important technique to analyze proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine steps to facilitate peptide identification. However, incomplete reactions and side reactions may occur, which compromise the experimental results. In...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00393e
更新日期:2017-11-21 00:00:00
abstract::It is now widely accepted that microRNAs (miRNAs or miRs) along with transcription factors (TFs) weave a complex inter-regulatory network within the cell that is responsible for the combinatorial regulation of gene expression. Recently we have shown that miRNAs and TFs that form network clusters are also associated wi...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25518b
更新日期:2013-06-01 00:00:00
abstract::Sponge metagenomes are accessible genetic sources containing genes and gene clusters responsible for the biosynthesis of sponge-derived bioactive natural products. In this study, we obtained the clone pDC112, producing turbomycin A and 2,2-di(3-indolyl)-3-indolone, based on the functional screening of the metagenome l...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00131e
更新日期:2015-05-01 00:00:00
abstract::Cellular heterogeneity and stochastic fluctuation play key roles in biological processes. Single molecule approaches have the key advantage of avoiding ensemble averaging, allowing the observation of transient intermediates and heterogeneity (both static and dynamic). Thus they have revolutionised the way many biologi...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b702845h
更新日期:2007-06-01 00:00:00
abstract::Large combinatorial libraries of macrocyclic peptides are a useful source of bioactive compounds. However, peptides are not generally cell permeable, so there is great interest in the development of methods to create large libraries of modified peptides. In particular, N-alkylation of peptides is known to improve thei...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00308c
更新日期:2015-10-01 00:00:00
abstract::Calcium (Ca(2+)) is used as a signaling molecule to regulate many cellular processes. Calcium signaling generally involves transient elevations of the concentration of free Ca(2+) in the cytosol. More pronounced and sustained elevations of intracellular Ca(2+) concentrations are observed during apoptosis (programmed c...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b920805d
更新日期:2010-05-01 00:00:00
abstract::Nitronyl nitroxides are capable of preventing cells, tissues, and organs from radical-induced damage through scavenging NO˙, ˙O(2)(-) and ˙OH. In order to explore the conversions of nitronyl nitroxides in biological systems with and without NO˙, HPLC-MS aided PC12 cell systems were developed, and the conversions of 2-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05037k
更新日期:2011-05-01 00:00:00