Production of indole antibiotics induced by exogenous gene derived from sponge metagenomes.

abstract：:The selective inhibition for JAK2 over the other JAK family kinases (JAK1, JAK3 and TYK2) has shared an immense challenge due to high conservatism. In this paper, the highly JAK2 selective mechanism of the thienopyridine derivative was identified at the molecular level, based on insights into the inhibitory effects of...

journal_title：Molecular bioSystems

authors： Li JJ,Cheng P,Tu J,Zhai HL,Zhang XY

更新日期：2016-02-01 00:00:00

abstract：:The interaction of Phospholipase D1 (PLD1) by its C-terminal domain D4 with PED/PEA15 has been indicated as a target for type 2 diabetes. PED/PEA15 is overexpressed in several tissues of individuals affected by type 2 diabetes and its overexpression in intact cells and in transgenic animal models impairs insulin regul...

journal_title：Molecular bioSystems

authors： Doti N,Cassese A,Marasco D,Paturzo F,Sabatella M,Viparelli F,Dathan N,Monti SM,Miele C,Formisano P,Beguinot F,Ruvo M

更新日期：2010-10-01 00:00:00

abstract：:Polyethylenimine (PEI, especially with M(w) of 25,000) has been known as an efficient gene carrier and a gold standard of gene transfection due to its high transfection efficiency (TE). However, high concomitant cytotoxicity limited the application of PEI. In this report, several cationic polymers derived from low mol...

journal_title：Molecular bioSystems

authors： Li S,Wang Y,Zhang J,Yang WH,Dai ZH,Zhu W,Yu XQ

更新日期：2011-04-01 00:00:00

abstract：:At the intersection between artificial intelligence and statistics, supervised learning allows algorithms to automatically build predictive models from just observations of a system. During the last twenty years, supervised learning has been a tool of choice to analyze the always increasing and complexifying data gene...

journal_title：Molecular bioSystems

authors： Geurts P,Irrthum A,Wehenkel L

更新日期：2009-12-01 00:00:00

abstract：:Gene regulatory networks constitute the first layer of the cellular computation for cell adaptation and surveillance. In these webs, a set of causal relations is built up from thousands of interactions between transcription factors and their target genes. The large size of these webs and their entangled nature make it...

journal_title：Molecular bioSystems

authors： Rodríguez-Caso C,Corominas-Murtra B,Solé RV

更新日期：2009-12-01 00:00:00

abstract：:Mannose-modified lectin-binding peptides were obtained from an α-helical-designed peptide phage library. Concanavalin A (ConA) was used as a representative target protein for the lectin family. The identified glycopeptides could selectively bind to ConA with micromolar affinity. With these results, the methodologies d...

journal_title：Molecular bioSystems

authors： Ven Chang I,Tsutsumi H,Mihara H

更新日期：2017-10-24 00:00:00

abstract：:The identification of disulfide bonds provides critical information regarding the structure and function of a protein and is a key aspect in understanding signaling cascades in biological systems. Recent proteomic approaches using digestion enzymes have facilitated the characterization of disulfide-bonds and/or oxidiz...

journal_title：Molecular bioSystems

authors： Na S,Paek E,Choi JS,Kim D,Lee SJ,Kwon J

更新日期：2015-04-01 00:00:00

abstract：:Individuals infected with the human immunodeficiency virus (HIV) often suffer from concomitant metabolic complications. Treatment with antiretroviral therapy has also been shown to alter the metabolism of patients. Although chemometric analysis of nuclear magnetic resonance (NMR) spectra of human sera can distinguish ...

journal_title：Molecular bioSystems

authors： McKnight TR,Yoshihara HA,Sitole LJ,Martin JN,Steffens F,Meyer D

更新日期：2014-11-01 00:00:00

abstract：:The chemical composition of the cervical mucus (CM), its physical characteristics and the volume of secretion change cyclically throughout the menstrual cycle. The aim of this study was to identify the constitutive protein composition of CM of fertile women and the changes in the CM proteome throughout the menstrual c...

journal_title：Molecular bioSystems

authors： Grande G,Milardi D,Vincenzoni F,Pompa G,Biscione A,Astorri AL,Fruscella E,De Luca A,Messana I,Castagnola M,Marana R

更新日期：2015-06-01 00:00:00

abstract：:Histone deacetylases (HDACs) contribute to regulation of gene expression by mediating higher-order chromatin structures. They assemble into large multiprotein complexes that regulate activity and specificity. We report the development of small molecule probes with class IIa and pan-HDAC activity that contain photoreac...

journal_title：Molecular bioSystems

authors： Albrow VE,Grimley RL,Clulow J,Rose CR,Sun J,Warmus JS,Tate EW,Jones LH,Storer RI

更新日期：2016-05-24 00:00:00

abstract：:Blockage of the interactions between immunologic checkpoint protein PD-1 and its ligand PD-L1 showed efficacy for cancer treatment. X-ray structures have captured static conformational snapshots of PD-1 and revealed that the CC' loop adopts an open conformation in the apo-protein but turns into a closed form and inter...

journal_title：Molecular bioSystems

authors： Liu W,Huang B,Kuang Y,Liu G

更新日期：2017-05-02 00:00:00

abstract：:Protein splicing technology harnesses the ability of inteins to ligate protein fragments, forming a mature protein. This report describes our effort to engineer rapamycin-dependent protein splicing of a ribotoxin, called α-sarcin. Engineering this system required the investigation of important splicing parameters, inc...

journal_title：Molecular bioSystems

authors： Alford SC,O'Sullivan C,Obst J,Christie J,Howard PL

更新日期：2014-04-01 00:00:00

abstract：:The complex web of interactions between the host immune system and the pathogen determines the outcome of any infection. A computational model of this interaction network, which encodes complex interplay among host and bacterial components, forms a useful basis for improving the understanding of pathogenesis, in filli...

journal_title：Molecular bioSystems

authors： Raman K,Bhat AG,Chandra N

更新日期：2010-03-01 00:00:00

abstract：:The non-classical zinc finger protein, Neural Zinc Finger Factor-1, contains six Cys2His2Cys domains. All three cysteines and the second histidine directly bind Zn(II). Using a combination of mutagenesis, metal coordination and DNA binding studies, we report that the first histidine is involved in a functionally impor...

journal_title：Molecular bioSystems

authors： Besold AN,Amick DL,Michel SL

更新日期：2014-07-01 00:00:00

abstract：:We explain that part of the reduction in the parameter uncertainties in the computations of Apgar et al. (Mol. Biosyst. 2010, 6, 1890-900) is due to a greatly increased number of effective data points. ...

journal_title：Molecular bioSystems

authors： Chachra R,Transtrum MK,Sethna JP

更新日期：2011-08-01 00:00:00

abstract：:Recently, more and more research has shown that microRNAs (miRNAs) play critical roles in the development and progression of various diseases, but it is not easy to predict potential human miRNA-disease associations from the vast amount of biological data. Computational methods for predicting potential disease-miRNA a...

journal_title：Molecular bioSystems

authors： Chen X,Liu MX,Yan GY

更新日期：2012-10-01 00:00:00

abstract：:The investigation of the structure and dynamics of signal transduction systems through data-based mathematical models in ordinary differential equations or other paradigms has proven to be a successful approach in recent times. Extending this concept, we here analysed the use of kinetic models based on power-law terms...

journal_title：Molecular bioSystems

authors： Vera J,Rath O,Balsa-Canto E,Banga JR,Kolch W,Wolkenhauer O

更新日期：2010-11-01 00:00:00

abstract：:New approaches to improve the quality and efficiency of stem cell-derived neurons are important for both research and clinical arenas. Customized subsets of differentiated neurons can be used as model systems to understand the etiology of a variety of complex neurological diseases and disorders. These same neuronal ce...

journal_title：Molecular bioSystems

authors： Greco SJ,Rameshwar P

更新日期：2010-02-01 00:00:00

abstract：:Induced hypoxia stress on cervical cancer derived cells (HeLa cells) leads to significant changes in their membrane lipid profiles. The lipidome of HeLa cells was characterized by a joint approach wherein liquid chromatography-mass spectrometry (LC-MS) analysis was followed by high resolution NMR measurements. Multiva...

journal_title：Molecular bioSystems

authors： Yu Y,Vidalino L,Anesi A,Macchi P,Guella G

更新日期：2014-04-01 00:00:00

abstract：:The size and complexity of cellular systems make building predictive models an extremely difficult task. In principle dynamical time-course data can be used to elucidate the structure of the underlying molecular mechanisms, but a central and recurring problem is that many and very different models can be fitted to exp...

journal_title：Molecular bioSystems

authors： Erguler K,Stumpf MP

更新日期：2011-05-01 00:00:00

abstract：:Protein-protein interactions play an essential role in the regulation of various cellular processes. Understanding the recognition mechanism of protein-protein complexes is a challenging task in molecular and computational biology. In this work, we have developed an energy based approach for identifying the binding si...

journal_title：Molecular bioSystems

authors： Gromiha MM,Yokota K,Fukui K

更新日期：2009-12-01 00:00:00

abstract：:In order to elucidate the effect of flexible linker length on the catalytic efficiency of fusion proteins, two short flexible peptide linkers of various lengths were fused between Arabidopsis thaliana 4-coumaroyl-CoA ligase (4CL) and Polygonum cuspidatum stilbene synthase (STS) to generate fusion proteins 4CL-(GSG)n-S...

journal_title：Molecular bioSystems

authors： Guo H,Yang Y,Xue F,Zhang H,Huang T,Liu W,Liu H,Zhang F,Yang M,Liu C,Lu H,Zhang Y,Ma L

更新日期：2017-02-28 00:00:00

abstract：:When exposed to low concentrations of toxic chemicals, bacteria modulate the expression of a number of cellular processes. Typically, these processes include those related to porin production, dismutases, and metabolic fluxes. In Escherichia coli (E. coli), the expression of these systems is largely controlled by thre...

journal_title：Molecular bioSystems

authors： Jain K,Saini S

更新日期：2016-05-24 00:00:00

abstract：:The multidrug resistance (MDR) system actively pumps antibiotics out of cells causing serious health problems. During the pumping, AcrB (one of the key components of MDR) undergoes a series of large-scale and proton-motive conformational changes. Capturing the conformational changes through all-atom simulations is cha...

journal_title：Molecular bioSystems

authors： Jewel Y,Liu J,Dutta P

更新日期：2017-09-26 00:00:00

abstract：:Precise quantification of endogenous protein-protein interactions across live cells would be a major boon to biology. Such precise measurement is theoretically possible with fluorescence lifetime imaging microscopy (FLIM) but requires first properly addressing multiple biological, instrumental, statistical, and photop...

journal_title：Molecular bioSystems

authors： Walther KA,Papke B,Sinn MB,Michel K,Kinkhabwala A

更新日期：2011-02-01 00:00:00

abstract：:Polycystic ovary syndrome (PCOS) is one of the most commonly occurring metabolic and endocrinological disorders affecting women of reproductive age. Metabolomics is an emerging field that holds promise in understanding disease pathophysiology. Recently, a few metabolomics based studies have been attempted in PCOS pati...

journal_title：Molecular bioSystems

authors： RoyChoudhury S,Mishra BP,Khan T,Chattopadhayay R,Lodh I,Datta Ray C,Bose G,Sarkar HS,Srivastava S,Joshi MV,Chakravarty B,Chaudhury K

更新日期：2016-10-18 00:00:00

abstract：:It is well known that oligomeric/aggregated amyloid β peptides are a key player in the pathogenesis of Alzheimer's disease and that different nanoparticles influence oligomerization/aggregation processes in experiments in vitro. Our previous results demonstrated antiaggregation effects of magnetite nanoparticles in th...

journal_title：Molecular bioSystems

authors： Gažová Z,Antošová A,Krištofiková Z,Bartoš A,Ríčný J,Cechová L,Klaschka J,Rípová D

更新日期：2010-11-01 00:00:00

abstract：:The induction of fungal metabolites by fungal co-cultures grown on solid media was explored using multi-well co-cultures in 2 cm diameter Petri dishes. Fungi were grown in 12-well plates to easily and rapidly obtain the large number of replicates necessary for employing metabolomic approaches. Fungal culture using suc...

journal_title：Molecular bioSystems

authors： Bertrand S,Azzollini A,Schumpp O,Bohni N,Schrenzel J,Monod M,Gindro K,Wolfender JL

更新日期：2014-07-29 00:00:00

abstract：:A major mode of signal transduction in bacteria is the two-component system, which involves phosphorylation of an output-generating receiver protein by a signal-sensing histidine kinase. This differs from the more common one-component system--where both signal sensing and output generation are performed by the same pr...

journal_title：Molecular bioSystems

authors： Seshasayee AS,Luscombe NM

更新日期：2011-11-01 00:00:00

abstract：:Transient and low-affinity interactions among macromolecules underlie many physiological events. Often, these interactions are difficult to study because they are not maintained when the participating molecules are removed from their cellular context. To circumvent this challenge, crosslinking reagents can be used to ...

journal_title：Molecular bioSystems

authors： Tanaka Y,Bond MR,Kohler JJ

更新日期：2008-06-01 00:00:00