Abstract:
:The neuroscientific community rapidly adopted RNA interference techniques as an experimental tool for the dissection of gene function in vitro and in animal models of neurological disease in vivo. Here, we discuss recent advances in the biotechnical implementation of siRNA/shRNA-mediated gene silencing focusing on issues of design, delivery and putative detrimental effects. We then summarize the current use of RNAi in targeting neurological disease models and give an outlook on the implementation of this technique in clinical therapy.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Lingor P,Bähr Mdoi
10.1039/b701169esubject
Has Abstractpub_date
2007-11-01 00:00:00pages
773-80issue
11eissn
1742-206Xissn
1742-2051journal_volume
3pub_type
杂志文章,评审abstract::Estrogen positive breast cancer is a dreadful disease in women worldwide. The human estrogen receptor-α (ERα) pathway plays a critical role in estrogenic signaling and targeting ERα in breast cancer treatment. The key role of Lemur tyrosine kinase-3 (LMTK3) in regulation of ERα has been identified and it is found to b...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00063c
更新日期:2014-05-01 00:00:00
abstract::J proteins are a diverse family of co-chaperones that cooperate with heat shock protein 70 (Hsp70) to coordinate protein quality control, especially in response to cellular stress. Current models suggest that individual J proteins might play roles in recruiting Hsp70s to specific functions, such as maintaining cell wa...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25248a
更新日期:2012-11-01 00:00:00
abstract::CC chemokine receptor 5 (CCR5), a member of G protein-coupled receptors (GPCRs), plays a vital role in inflammatory responses to infection. Alterations in the expression of CCR5 have been correlated with disease progression in many types of cancers. The idea of using CCR5 as a target for therapeutic intervention has b...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00577b
更新日期:2016-10-18 00:00:00
abstract::As a versatile pathogen Staphylococcus aureus can cause various disease patterns, which are influenced by strain specific virulence factor repertoires but also by S. aureus physiological adaptation capacity. Here, we present metabolomic descriptions of S. aureus central metabolic pathways and demonstrate the potential...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00315h
更新日期:2011-04-01 00:00:00
abstract::The major mechanical function of talin is to couple the β-integrin cytoplasmic tails to actin filaments. A variety of β-integrin tails contain conserved binding motifs for talin, and recent research shows that β-integrins differ both in affinity to talin and preferences for other cytoplasmic adaptor proteins. While ta...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00341a
更新日期:2014-12-01 00:00:00
abstract::Genetic information in genomes is ordered, arranged in such a way that it constitutes a code, the so-called cis regulatory code. The regulatory machinery of the cell, termed trans-factors, decodes and expresses this information. In this way, genomes maintain a potential repertoire of genetic programs, parts of which a...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c6mb00789a
更新日期:2017-03-28 00:00:00
abstract::A major effort in systems biology is the development of mathematical models that describe complex biological systems at multiple scales and levels of abstraction. Determining the topology-the set of interactions-of a biological system from observations of the system's behavior is an important and difficult problem. He...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00276h
更新日期:2015-02-01 00:00:00
abstract::In the pathogenesis of renal fibrosis, oxidative stress (OS) enhances the production of reactive oxygen species (ROS) leading to sustained cell growth, inflammation, excessive tissue remodelling and accumulation, which results in the development and acceleration of renal damage. In our previous work (Eltoweissy et al....
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00887e
更新日期:2016-05-24 00:00:00
abstract::Synthetic studies of lipid A and LPS partial structures have been performed to investigate the relationship between structures and functions of LPS. Recent studies have suggested several pathological implications of LPS from parasitic bacteria due to its influence on the host immune responses. To address this issue, w...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25477a
更新日期:2013-05-01 00:00:00
abstract::Proteins are dynamic creatures. Intrinsically disordered proteins (IDPs) function as multiplicity of structures and their activities can only be described by stochastic structure-function relationships. In their complex forms, however, IDPs were thought to lose their plasticity and behave similarly to globular protein...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c1mb05234a
更新日期:2012-01-01 00:00:00
abstract::Protein phosphorylation is associated with most cell signaling and developmental processes in eukaryotes. Despite the vast extent of the phosphoproteome within the cell, connecting specific kinases with relevant targets remains a significant experimental frontier. The challenge of linking kinases and their substrates ...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b801724g
更新日期:2008-09-01 00:00:00
abstract::To faithfully execute diverse biological programs all cells need to access and distribute their genomes in a highly organized way. In the nucleus of eukaryotic cells DNA is packed with histone proteins into chromatin. The originating nucleo-protein complex is the regulatory platform for all genetic processes. Of these...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c2mb25328c
更新日期:2013-02-02 00:00:00
abstract::A metabolomic study was performed to investigate the biochemical profiles of livers from male and female zebrafish (Danio rerio), using a multiple platform approach, incorporating 1H NMR, GC/MS and LC/MS. The reproducibility and reliability of the three methods were validated prior to the assays. Major biomolecules de...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b811850g
更新日期:2009-03-01 00:00:00
abstract::Drosophila cell culture is used as a model system with multiple applications including the identification of new therapeutic targets in screens, the study of conserved signal transduction pathway mechanisms, and as an expression system for recombinant proteins. However, in vitro methods for Drosophila cell and organ c...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00155a
更新日期:2014-10-01 00:00:00
abstract::Production of biofuels has been one of the promising efforts in biotechnology in the past few decades. The perspective of these efforts can be reduction of increasing demands for fossil fuels and consequently reducing environmental pollution. Nonetheless, most previous approaches did not succeed in obviating many big ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00119j
更新日期:2016-07-19 00:00:00
abstract::Conventional methods for detecting single nucleotide polymorphisms (SNPs), including direct DNA sequencing, pyrosequencing, and melting curve analysis, are to a great extent limited by their requirement for particular detection instruments. To overcome this limitation, we established a novel SNP detection technique ut...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b701645j
更新日期:2007-08-01 00:00:00
abstract::The crystal structure of the versatile CYP109B1 enzyme from Bacillus subtilis has been solved at 1.8 Å resolution. This is the first structure of an enzyme from this CYP family, whose members are prevalent across diverse species of bacteria. In the crystal structure the enzyme has an open conformation with an access c...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00665h
更新日期:2015-03-01 00:00:00
abstract::A metabolomic method using reversed-phase liquid chromatography/quadrupole time-of-flight mass spectrometry (LC-Q-TOF-MS) was developed to obtain a systematic view of the development and progression of myocardial infarction (MI). By combining with partial least squares discriminant analysis (PLS-DA), 16 biomarkers in ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00110d
更新日期:2011-03-01 00:00:00
abstract::A cyclic dinucleotide riboswitch has been fused with a G-quadruplex motif to produce a conditional riboswitch-peroxidase-mimicking sensor that oxidizes both colorimetric and fluorogenic substrates in the presence of c-di-GMP. We find that signal-to-noise ratio could be improved by using a two-, not three-, floor split...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00751h
更新日期:2016-03-01 00:00:00
abstract::The eIF4E-binding protein 1 (4EBP1) has long been known to be completely unstructured without any secondary structures, which contributed significantly to the proposal of the induced fit mechanism for target binding of intrinsically disordered proteins. We show here that 4EBP1 is not completely unstructured, but conta...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00532e
更新日期:2015-02-01 00:00:00
abstract::Experimental data from biological pathways come in many forms: qualitative or quantitative, static or dynamic. By combining a variety of these heterogeneous sources of data, we construct a mathematical model of a critical regulatory network in vertebrate development, the Sonic Hedgehog signaling pathway. The structure...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00006j
更新日期:2010-10-01 00:00:00
abstract::A novel class of pH-sensitive PEG lipids bearing acid-cleavable acetal linkages and short PEG chains have been synthesised and used in ternary vector formulations. The cleavage pH was influenced by structural components including the terminal PEG moiety and spacer length. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b719782a
更新日期:2008-06-01 00:00:00
abstract::We explain that part of the reduction in the parameter uncertainties in the computations of Apgar et al. (Mol. Biosyst. 2010, 6, 1890-900) is due to a greatly increased number of effective data points. ...
journal_title:Molecular bioSystems
pub_type: 评论,信件
doi:10.1039/c1mb05046j
更新日期:2011-08-01 00:00:00
abstract::Stochastic fluctuations (noise) in gene expression can cause members of otherwise genetically identical populations to display drastically different phenotypes. An understanding of the sources of noise and the strategies cells employ to function reliably despite noise is proving to be increasingly important in describ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b801245h
更新日期:2008-07-01 00:00:00
abstract::Recently, more and more research has shown that microRNAs (miRNAs) play critical roles in the development and progression of various diseases, but it is not easy to predict potential human miRNA-disease associations from the vast amount of biological data. Computational methods for predicting potential disease-miRNA a...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25180a
更新日期:2012-10-01 00:00:00
abstract::Cells of the bacterium Myxococcus xanthus organize into two types of patterns depending on their nutritional status, i.e. in the presence of nutrients cells form spreading colonies and in the absence of nutrients cells form fruiting bodies. Formation of both patterns depends on directed cell movements, which, in turn,...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b806640j
更新日期:2008-10-01 00:00:00
abstract::The Parkinson's disease (PD) associated gene PINK1 encodes a protein kinase that mediates the phosphorylation of multiple proteins involved in mitochondrial homeostasis. The broader downstream signaling events mediated by PINK1 kinase activity have not been well documented. We combine quantitative phosphoproteomic str...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70565j
更新日期:2014-07-01 00:00:00
abstract::Type 2 diabetes mellitus (T2DM) is increased worldwide in parallel with the obesity epidemic. Potentilla discolor is one of the most important crude materials in Traditional Chinese medicine (TCM) for therapy of hyperglycemia and hyperlipidemia. In this work, a plasma metabonomic approach based on the combination of U...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00278d
更新日期:2014-11-01 00:00:00
abstract::Although angiotensin (Ang)II-induced Janus-activated kinase (JAK)2 phosphorylation was reported to be enhanced in failing human cardiomyocytes, the downstream balance between cardio-protective (signal transducer and activator of transcription-STAT3) and the pro-inflammatory (STAT2 and STAT5) response remains unexplore...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25120e
更新日期:2012-09-01 00:00:00
abstract::Major progress in the study of RNase P has resulted from crystallography of bacterial catalytic subunits and the discovery of catalytic activity in eukaryotes. Several new substrates have also been identified, primarily in bacteria but also in yeast. Our current world should be called the "RNA-protein world" rather th...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b707850c
更新日期:2007-09-01 00:00:00