Abstract:
:As the usage of fluorescence microscopy as a tool to study biological systems continues to grow, so does the need for additional tools that permit the selective detection of proteins of interest. Existing selective and well-characterized kinase inhibitors may be exploited to develop novel small molecule probes useful in imaging kinases by fluorescence microscopy.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Zhang Z,Kwiatkowski N,Zeng H,Lim SM,Gray NS,Zhang W,Yang PLdoi
10.1039/c2mb25099csubject
Has Abstractpub_date
2012-10-01 00:00:00pages
2523-6issue
10eissn
1742-206Xissn
1742-2051journal_volume
8pub_type
杂志文章abstract::Plant aquaporins belong to a large superfamily of conserved proteins called the major intrinsic proteins (MIPs). There is limited information about the diversity of MIPs and their water transport capacity in broccoli (Brassica oleracea) plants. In this study, the cDNAs of isoforms of Plasma Membrane Intrinsic Proteins...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00285b
更新日期:2011-04-01 00:00:00
abstract::The importance of microRNA (miRNA) in modulating gene expression at the post-transcriptional level is well known. Such regulation has been shown to influence the dynamics of several regulatory networks including the cell cycle. In this study we incorporated regulatory effects of intronic miRNA into an existing mathema...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25046b
更新日期:2012-08-01 00:00:00
abstract::The major obstacle for the development of targeted therapies is the lack of pharmacodynamic (PD) biomarkers to provide an early readout of biological activities. As the modulation of metabolites may reflect the biological changes occurring in the targets, metabolomics is promising to be an efficient way to explore PD ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c7mb00093f
更新日期:2017-07-25 00:00:00
abstract::Thyroid carcinoma is a common endocrine malignancy worldwide, accounting for approximately 1% of all diagnosed cancers and about 91.5% of the malignancies of head and neck. However, differentiating malignant thyroid nodules from benign ones remains a diagnostic challenge. Thus, novel molecular markers that enable non-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05029j
更新日期:2011-09-01 00:00:00
abstract::G protein-coupled receptors (GPCRs) are key signaling proteins that regulate how cells interact with their environment. Traditional signaling cascades involving GPCRs have been well described and are well established and very important clinical targets. With the development of more recent technologies, hints about the...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c2mb25429h
更新日期:2013-04-05 00:00:00
abstract::Although angiotensin (Ang)II-induced Janus-activated kinase (JAK)2 phosphorylation was reported to be enhanced in failing human cardiomyocytes, the downstream balance between cardio-protective (signal transducer and activator of transcription-STAT3) and the pro-inflammatory (STAT2 and STAT5) response remains unexplore...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25120e
更新日期:2012-09-01 00:00:00
abstract::The aim of this study was to investigate the cellular lipid metabolism associated with β-amyloid peptide (Aβ)-induced neurotoxicity as well as the neuroprotective effect of (-)-epigallocatechin gallate (EGCG), a major polyphenol in green tea. An ultra-performance liquid chromatography-quadrupole-time-of-flight mass sp...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25126d
更新日期:2012-10-30 00:00:00
abstract::Over the last few years important new insights into the process of long-range gene regulation have been obtained. Gene regulatory elements are found to engage in direct physical interactions with distant target genes and with loci on other chromosomes to modulate transcription. An overview of recently discovered long-...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b803580f
更新日期:2008-11-01 00:00:00
abstract::Celastrol, isolated from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f. (Thunder God's Vine), has been used to treat cancer, chronic inflammatory, autoimmune and other human diseases. However, to date, the protein targets and the mechanism of action of celastrol have remained elusive. In this s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00691d
更新日期:2016-12-20 00:00:00
abstract::Biochemical modifications to mRNA, especially N6-methyladenosine (m6A) and 5-methylcytosine (m5C), have been recently shown to be associated with crucial biological functions. Despite the intriguing advancements, little is known so far about the dynamic landscape of RNA methylome across different cell types and how th...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00604f
更新日期:2015-01-01 00:00:00
abstract::To improve the performance of nanostructured calcium carbonate in gene delivery, a hydrophilic polysaccharide, alginate, was added to calcium carbonate co-precipitation systems to form alginate/CaCO(3)/DNA nanoparticles. The size and ζ-potential of the nanoparticles were measured by a zetasizer. Due to the existence o...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05337j
更新日期:2012-03-01 00:00:00
abstract::Sponge metagenomes are accessible genetic sources containing genes and gene clusters responsible for the biosynthesis of sponge-derived bioactive natural products. In this study, we obtained the clone pDC112, producing turbomycin A and 2,2-di(3-indolyl)-3-indolone, based on the functional screening of the metagenome l...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00131e
更新日期:2015-05-01 00:00:00
abstract::The heterogeneity of genetic alterations in human cancer genomes presents a major challenge to advancing our understanding of cancer mechanisms and identifying cancer driver genes. To tackle this heterogeneity problem, many approaches have been proposed to investigate genetic alterations and predict driver genes at th...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25528f
更新日期:2013-03-01 00:00:00
abstract::A recent model proposing that a barrier is raised against tumor evolution in pre-cancer tissues is investigated. For that we quantify expression alterations in genome maintenance pathways: DNA damage response, death pathways and cell cycle and also differentially expressed genes in transcriptomes of pre-cancerous and ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25242b
更新日期:2012-11-01 00:00:00
abstract::Osteosarcoma (OS) is a highly malignant bone tumour, affecting mainly children and young adults between 10 and 20 years of age. It represents the most frequent primitive malignant tumour of the skeletal system and is characterized by an extremely aggressive clinical course, with rapid development of lung metastases. I...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70328b
更新日期:2014-06-01 00:00:00
abstract::Undoubtedly, metabonomics can reveal the comprehensive efficacies of traditional Chinese medicine (TCM) formulae and its complex mechanism at the molecular biological level. In this study, an attempt was made to address the pretreatment effect of a TCM formula. In this case, as a critical point, we should first know h...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00529e
更新日期:2015-02-01 00:00:00
abstract::With the advent of new cancer therapies in the last few years, the goals of reducing disease burden and improving quality of life are frequently achieved. Yet despite the advances seen with numerous monotherapies, a multimodality approach that targets different aspects of tumor biology may yield the greatest clinical ...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b911313b
更新日期:2009-11-01 00:00:00
abstract::The identification of a suitable non-fluorescent molecule that can be oxidized by G-quadruplexes into a fluorescent product will be important for several fields such as bioanalyte sensing and cancer therapeutic discovery. Herein, we demonstrate that 2',7'-dichlorodihydrofluorescein diacetate is a superior reducing sub...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b916228c
更新日期:2010-01-01 00:00:00
abstract::Nicotinamide adenine dinucleotide (NAD) is a ubiquitous cofactor participating in numerous redox reactions. It is also a substrate for regulatory modifications of proteins and nucleic acids via the addition of ADP-ribose moieties or removal of acyl groups by transfer to ADP-ribose. In this study, we use in-depth seque...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb05487f
更新日期:2012-06-01 00:00:00
abstract::Establishing functional relationships between multi-domain protein sequences is a non-trivial task. Traditionally, delineating functional assignment and relationships of proteins requires domain assignments as a prerequisite. This process is sensitive to alignment quality and domain definitions. In multi-domain protei...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70443b
更新日期:2014-05-01 00:00:00
abstract::We have investigated the thermodynamics of the interaction between the TATA-box-binding protein from Pyrococcus horikoshii (PhoTBP) and its target DNA (TATA-1). The interaction between PhoTBP and double-stranded DNA (dsDNA) is entropically favorable and enthalpically unfavorable. The thermodynamic parameters for TATA-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b904200h
更新日期:2009-09-01 00:00:00
abstract::Cycloserine (CS, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (PLP)-dependent enzymes. Two CS enantiomers are known; D-cycloserine (DCS, also known as Seromycin) is a natural product that is used to treat resistant Mycobacterium tuberculosis infec...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c003743e
更新日期:2010-09-01 00:00:00
abstract::The non-classical zinc finger protein, Neural Zinc Finger Factor-1, contains six Cys2His2Cys domains. All three cysteines and the second histidine directly bind Zn(II). Using a combination of mutagenesis, metal coordination and DNA binding studies, we report that the first histidine is involved in a functionally impor...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00246f
更新日期:2014-07-01 00:00:00
abstract::In actively proliferating cells, such as the cells of the developing embryo, DNA repair is crucial for preventing the accumulation of mutations and synchronizing cell division. Sea urchin embryo growth was analyzed and extracts were prepared. The relative activity of DNA polymerase, apurinic/apyrimidinic (AP) endonucl...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00906e
更新日期:2016-06-21 00:00:00
abstract::Low transfection efficiency and inactivation by serum are the major drawbacks for cationic polymers when used as non-viral gene vectors. Here, a series of N-succinyl-chitosan-graft-polyethylenimine (NSC-g-PEI) copolymers with different compositions were synthesized through grafting low molecular weight PEI (800 Da) to...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b822505b
更新日期:2009-06-01 00:00:00
abstract::Protein methylation catalyzed by methyltransferases carries many important biological functions. Methylation and their regulatory enzymes are involved in a variety of human disease states, raising the possibility that abnormally methylated proteins can be disease markers and methyltransferases are potential therapeuti...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c5mb00259a
更新日期:2015-10-01 00:00:00
abstract::Primarily used for medicinal purposes in the past, biologically active small molecules have been increasingly employed to explore complex biological processes in the era of "chemical genetics". Since the contributions of this small molecule approach to biology have been extensive, we limit the focus of our review to t...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b716221a
更新日期:2008-04-01 00:00:00
abstract::Information on extracellular signals and conditions is often transduced by biological systems using cascades of protein phosphorylation that affect the activity of enzymes, the localization of proteins and gene expression. A model to study signal transduction is the response of the yeast Saccharomyces cerevisiae to os...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b902256b
更新日期:2009-11-01 00:00:00
abstract::Insight into the molecular mechanism of complex diseases is an important topic in the current bio-medical research. However, different from the single-gene disorders, high heterogeneity of many of the complex diseases prevents scientists from the exact understanding of the etiology. In this study, we used Myelodysplas...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05018d
更新日期:2011-06-01 00:00:00
abstract::Despite many prior studies on microbial response to oxidative stress, our understanding of microbial tolerance against oxidative stress is currently limited to aerobic conditions, and few engineering strategies have been devised to resolve toxicity issues of oxidative stress under anaerobic conditions. Since biologica...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25259g
更新日期:2013-02-02 00:00:00