An intein-cassette integration approach used for the generation of a split TEV protease activated by conditional protein splicing.

abstract：:There are major issues regarding the proposed pathway for starch degradation in germinating cereal grain. Given the commercial importance but genetic intractability of the problem, we have embarked on a program of chemical genetics studies to identify and dissect the pathway and regulation of starch degradation in ger...

journal_title：Molecular bioSystems

authors： Rejzek M,Stevenson CE,Southard AM,Stanley D,Denyer K,Smith AM,Naldrett MJ,Lawson DM,Field RA

更新日期：2011-03-01 00:00:00

abstract：:Bacterial resistance to β-lactam antibiotics poses a serious threat to human health. Penicillin binding proteins (PBPs) and β-lactamases are involved in both antibacterial activity and mediation of β-lactam antibiotic resistance. The two major reasons for resistance to β-lactams include: (i) pathogenic bacteria expres...

journal_title：Molecular bioSystems

authors： Kumar KM,Anbarasu A,Ramaiah S

更新日期：2014-04-01 00:00:00

abstract：:Recently, more and more research has shown that microRNAs (miRNAs) play critical roles in the development and progression of various diseases, but it is not easy to predict potential human miRNA-disease associations from the vast amount of biological data. Computational methods for predicting potential disease-miRNA a...

journal_title：Molecular bioSystems

authors： Chen X,Liu MX,Yan GY

更新日期：2012-10-01 00:00:00

abstract：:Oxygen dependent modulation of red blood cell metabolism is a long investigated issue. However, the recent introduction of novel mass spectrometry-based approaches lends itself to implement our understanding of the effects of red blood cell prolonged exposure to anaerobiosis. Indeed, most of the studies conducted so f...

journal_title：Molecular bioSystems

authors： D'Alessandro A,Gevi F,Zolla L

更新日期：2013-06-01 00:00:00

abstract：:Sequence specific binding of DNA and RNA is of fundamental importance in the regulation of cellular gene expression. Because of their modular structure repeat domain proteins are particularly well suited for these processes and have been widely adopted throughout evolution. Detailed biochemical and structural data has...

journal_title：Molecular bioSystems

authors： Filipovska A,Rackham O

更新日期：2012-03-01 00:00:00

abstract：:Upon rapid ascent to a high altitude, non-acclimatized individuals, although healthy, are highly prone to contracting high-altitude pulmonary edema (HAPE). Early diagnosis is difficult and there is no reliable biomarker available. We used proton ((1)H) NMR metabolomics to profile the altered metabolic patterns of bloo...

journal_title：Molecular bioSystems

authors： Luo Y,Zhu J,Gao Y

更新日期：2012-06-01 00:00:00

abstract：:The interaction of spermine and its analogue, 1-naphthyl acetyl spermine with four double stranded DNA polynucleotides has been studied to understand the structural and thermodynamic basis of the binding. The efficacy and specificity of DNA binding of this analogue has not yet been revealed. The energetics of the inte...

journal_title：Molecular bioSystems

authors： Kabir A,Kumar GS

更新日期：2014-05-01 00:00:00

abstract：:Induced hypoxia stress on cervical cancer derived cells (HeLa cells) leads to significant changes in their membrane lipid profiles. The lipidome of HeLa cells was characterized by a joint approach wherein liquid chromatography-mass spectrometry (LC-MS) analysis was followed by high resolution NMR measurements. Multiva...

journal_title：Molecular bioSystems

authors： Yu Y,Vidalino L,Anesi A,Macchi P,Guella G

更新日期：2014-04-01 00:00:00

abstract：:Drug promiscuity is one of the key issues in current drug development. Many famous drugs have turned out to behave unexpectedly due to their propensity to bind to multiple targets. One of the primary reasons for this promiscuity is that drugs bind to multiple distinctive target environments, a feature that we call mul...

journal_title：Molecular bioSystems

authors： Park K,Lee S,Ahn HS,Kim D

更新日期：2009-08-01 00:00:00

abstract：:New approaches to improve the quality and efficiency of stem cell-derived neurons are important for both research and clinical arenas. Customized subsets of differentiated neurons can be used as model systems to understand the etiology of a variety of complex neurological diseases and disorders. These same neuronal ce...

journal_title：Molecular bioSystems

authors： Greco SJ,Rameshwar P

更新日期：2010-02-01 00:00:00

abstract：:Cycloserine (CS, 4-amino-3-isoxazolidone) is a cyclic amino acid mimic that is known to inhibit many essential pyridoxal 5'-phosphate (PLP)-dependent enzymes. Two CS enantiomers are known; D-cycloserine (DCS, also known as Seromycin) is a natural product that is used to treat resistant Mycobacterium tuberculosis infec...

journal_title：Molecular bioSystems

authors： Lowther J,Yard BA,Johnson KA,Carter LG,Bhat VT,Raman MC,Clarke DJ,Ramakers B,McMahon SA,Naismith JH,Campopiano DJ

更新日期：2010-09-01 00:00:00

abstract：:Polyethylenimine (PEI, especially with M(w) of 25,000) has been known as an efficient gene carrier and a gold standard of gene transfection due to its high transfection efficiency (TE). However, high concomitant cytotoxicity limited the application of PEI. In this report, several cationic polymers derived from low mol...

journal_title：Molecular bioSystems

authors： Li S,Wang Y,Zhang J,Yang WH,Dai ZH,Zhu W,Yu XQ

更新日期：2011-04-01 00:00:00

abstract：:The effect of the glutathione transferase P1-1 (GSTP1-1) targeting has been investigated in both sensitive (U-2OS) and cisplatin-resistant (U-2OS/CDDP4 μg) human osteosarcoma cell lines. Despite the different enzyme's content, inhibition of GSTP1-1 by 6-(7-nitro-2,1,3-benzoxadiazol-4-ylthio)hexanol (NBDHEX) causes the...

journal_title：Molecular bioSystems

authors： Sau A,Filomeni G,Pezzola S,D'Aguanno S,Tregno FP,Urbani A,Serra M,Pasello M,Picci P,Federici G,Caccuri AM

更新日期：2012-04-01 00:00:00

abstract：:At the intersection between artificial intelligence and statistics, supervised learning allows algorithms to automatically build predictive models from just observations of a system. During the last twenty years, supervised learning has been a tool of choice to analyze the always increasing and complexifying data gene...

journal_title：Molecular bioSystems

authors： Geurts P,Irrthum A,Wehenkel L

更新日期：2009-12-01 00:00:00

abstract：:Insights into the structure and dynamics of modular polyketide synthases (PKS) are essential for understanding the mechanistic details of the biosynthesis of a large number of pharmaceutically important secondary metabolites. The crystal structures of the KS-AT di-domain from erythromycin synthase have revealed the re...

journal_title：Molecular bioSystems

authors： Anand S,Mohanty D

更新日期：2012-04-01 00:00:00

abstract：:The importance of microRNA (miRNA) in modulating gene expression at the post-transcriptional level is well known. Such regulation has been shown to influence the dynamics of several regulatory networks including the cell cycle. In this study we incorporated regulatory effects of intronic miRNA into an existing mathema...

journal_title：Molecular bioSystems

authors： Gokhale S,Hariharan M,Brahmachari SK,Gadgil C

更新日期：2012-08-01 00:00:00

abstract：:Abundant information about the nucleotide sequence of the human genome has become readily available and it is now necessary to develop methods for the identification of genes that are involved in important cellular, developmental and disease-related processes. Identification methods based on the activities of hammerhe...

journal_title：Molecular bioSystems

authors： Sano M,Kato Y,Taira K

更新日期：2005-05-01 00:00:00

abstract：:The Parkinson's disease (PD) associated gene PINK1 encodes a protein kinase that mediates the phosphorylation of multiple proteins involved in mitochondrial homeostasis. The broader downstream signaling events mediated by PINK1 kinase activity have not been well documented. We combine quantitative phosphoproteomic str...

journal_title：Molecular bioSystems

authors： Qin X,Zheng C,Yates JR 3rd,Liao L

更新日期：2014-07-01 00:00:00

abstract：:CC chemokine receptor 5 (CCR5), a member of G protein-coupled receptors (GPCRs), plays a vital role in inflammatory responses to infection. Alterations in the expression of CCR5 have been correlated with disease progression in many types of cancers. The idea of using CCR5 as a target for therapeutic intervention has b...

journal_title：Molecular bioSystems

authors： Wang J,Shu M,Wang Y,Hu Y,Wang Y,Luo Y,Lin Z

更新日期：2016-10-18 00:00:00

abstract：:With the advent of new cancer therapies in the last few years, the goals of reducing disease burden and improving quality of life are frequently achieved. Yet despite the advances seen with numerous monotherapies, a multimodality approach that targets different aspects of tumor biology may yield the greatest clinical ...

journal_title：Molecular bioSystems

authors： Kamrava M,Bernstein MB,Camphausen K,Hodge JW

更新日期：2009-11-01 00:00:00

abstract：:To improve the performance of nanostructured calcium carbonate in gene delivery, a hydrophilic polysaccharide, alginate, was added to calcium carbonate co-precipitation systems to form alginate/CaCO(3)/DNA nanoparticles. The size and ζ-potential of the nanoparticles were measured by a zetasizer. Due to the existence o...

journal_title：Molecular bioSystems

authors： Zhao D,Zhuo RX,Cheng SX

更新日期：2012-03-01 00:00:00

abstract：:Celastrol, isolated from the traditional Chinese medicinal herb Tripterygium wilfordii Hook. f. (Thunder God's Vine), has been used to treat cancer, chronic inflammatory, autoimmune and other human diseases. However, to date, the protein targets and the mechanism of action of celastrol have remained elusive. In this s...

journal_title：Molecular bioSystems

authors： Zhou Y,Li W,Wang M,Zhang X,Zhang H,Tong X,Xiao Y

更新日期：2016-12-20 00:00:00

abstract：:It is well known that oligomeric/aggregated amyloid β peptides are a key player in the pathogenesis of Alzheimer's disease and that different nanoparticles influence oligomerization/aggregation processes in experiments in vitro. Our previous results demonstrated antiaggregation effects of magnetite nanoparticles in th...

journal_title：Molecular bioSystems

authors： Gažová Z,Antošová A,Krištofiková Z,Bartoš A,Ríčný J,Cechová L,Klaschka J,Rípová D

更新日期：2010-11-01 00:00:00

abstract：:The major obstacle for the development of targeted therapies is the lack of pharmacodynamic (PD) biomarkers to provide an early readout of biological activities. As the modulation of metabolites may reflect the biological changes occurring in the targets, metabolomics is promising to be an efficient way to explore PD ...

journal_title：Molecular bioSystems

authors： Fang J,Wang L,Wang Y,Qiu M,Zhang Y

更新日期：2017-07-25 00:00:00

abstract：:Mass spectrometry (MS) has become an increasingly important technique to analyze proteins. In popular bottom-up MS-based proteomics, reduction and alkylation are routine steps to facilitate peptide identification. However, incomplete reactions and side reactions may occur, which compromise the experimental results. In...

journal_title：Molecular bioSystems

authors： Suttapitugsakul S,Xiao H,Smeekens J,Wu R

更新日期：2017-11-21 00:00:00

abstract：:Regular physical activity is associated with a reduced risk of coronary heart disease, as it probably modifies the balance between free-radical generation and antioxidant activity. On the other hand, however, acute physical activity increases oxygen uptake and leads to a temporary imbalance between the production of r...

journal_title：Molecular bioSystems

authors： Guidi F,Magherini F,Gamberi T,Bini L,Puglia M,Marzocchini R,Ranaldi F,Modesti PA,Gulisano M,Modesti A

更新日期：2011-03-01 00:00:00

abstract：:Comparative analysis of the complete genome sequences from a variety of poorly studied organisms aims at predicting ecological and behavioral properties of these organisms and helping in characterizing their habitats. This task requires finding appropriate descriptors that could be correlated with the core traits of e...

journal_title：Molecular bioSystems

authors： Galperin MY,Higdon R,Kolker E

更新日期：2010-04-01 00:00:00

abstract：:Metabolomics is a rapidly growing branch of post-genomic chemical biology. The fission yeast Schizosaccharomyces pombe is an excellent eukaryotic model organism. Although the entire S. pombe genome has been sequenced and detailed transcriptomic analyses were performed, little metabolic profiling has been done. Here we...

journal_title：Molecular bioSystems

authors： Pluskal T,Nakamura T,Villar-Briones A,Yanagida M

更新日期：2010-01-01 00:00:00

abstract：:Inferring metabolic networks from metabolite concentration data is a central topic in systems biology. Mathematical techniques to extract information about the network from data have been proposed in the literature. This paper presents a critical assessment of the feasibility of reverse engineering of metabolic networ...

journal_title：Molecular bioSystems

authors： Hendrickx DM,Hendriks MM,Eilers PH,Smilde AK,Hoefsloot HC

更新日期：2011-02-01 00:00:00

abstract：:Calcium (Ca(2+)) is used as a signaling molecule to regulate many cellular processes. Calcium signaling generally involves transient elevations of the concentration of free Ca(2+) in the cytosol. More pronounced and sustained elevations of intracellular Ca(2+) concentrations are observed during apoptosis (programmed c...

journal_title：Molecular bioSystems

authors： Brnjic S,Olofsson MH,Havelka AM,Linder S

更新日期：2010-05-01 00:00:00