Abstract:
:Abundant information about the nucleotide sequence of the human genome has become readily available and it is now necessary to develop methods for the identification of genes that are involved in important cellular, developmental and disease-related processes. Identification methods based on the activities of hammerhead and hairpin ribozymes and of short hairpin RNAs (shRNAs), whose target specificities are coupled with loss-of-function phenotypes, have received increasing attention as possible tools for the rapid identification of key genes involved in such processes. We describe here recent advances that have been made with libraries of ribozymes and shRNAs and compare the advantages of the different types of library. The use of such libraries has already revealed new details of several important physiological phenomena.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Sano M,Kato Y,Taira Kdoi
10.1039/b503235ksubject
Has Abstractpub_date
2005-05-01 00:00:00pages
27-35issue
1eissn
1742-206Xissn
1742-2051journal_volume
1pub_type
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