Functional gene-discovery systems based on libraries of hammerhead and hairpin ribozymes and short hairpin RNAs.

Abstract:

:Abundant information about the nucleotide sequence of the human genome has become readily available and it is now necessary to develop methods for the identification of genes that are involved in important cellular, developmental and disease-related processes. Identification methods based on the activities of hammerhead and hairpin ribozymes and of short hairpin RNAs (shRNAs), whose target specificities are coupled with loss-of-function phenotypes, have received increasing attention as possible tools for the rapid identification of key genes involved in such processes. We describe here recent advances that have been made with libraries of ribozymes and shRNAs and compare the advantages of the different types of library. The use of such libraries has already revealed new details of several important physiological phenomena.

journal_name

Mol Biosyst

journal_title

Molecular bioSystems

authors

Sano M,Kato Y,Taira K

doi

10.1039/b503235k

subject

Has Abstract

pub_date

2005-05-01 00:00:00

pages

27-35

issue

1

eissn

1742-206X

issn

1742-2051

journal_volume

1

pub_type

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