Abstract:
:Protein tyrosine phosphatases are crucial regulators of signal transduction and function as antagonists towards protein tyrosine kinases to control reversible tyrosine phosphorylation, thereby regulating fundamental physiological processes. Growing evidence has supported the notion that reversible oxidative inactivation of the catalytic cysteine residue in protein tyrosine phosphatases serves as an oxidative post-translational modification that regulates its activity to influence downstream signaling by promoting phosphorylation and induction of the signaling cascade. The oxidation of cysteine to the sulfenic acid is often transient and difficult to detect, thus making it problematic in understanding the role that this oxidative post-translational modification plays in redox-biology and pathogenesis. Several methods to detect cysteine oxidation in biological systems have been developed, though targeted approaches to directly detect oxidized phosphatases are still lacking. Herein we describe the development of a novel immunochemical approach to directly profile oxidized phosphatases. This immunochemical approach consists of an antibody designed to recognize the conserved sequence of the PTP active site (VHCDMDSAG) harboring the catalytic cysteine modified with dimedone (CDMD), a nucleophile that chemoselectively reacts with cysteine sulfenic acids to form a stable thioether adduct. Additionally, we provide biochemical and mass spectrometry workflows to be used in conjugation with this newly developed immunochemical approach to assist in the identification and quantification of basal and oxidized phosphatases.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Garcia FJ,Carroll KSdoi
10.1039/c5mb00847fsubject
Has Abstractpub_date
2016-05-24 00:00:00pages
1790-8issue
6eissn
1742-206Xissn
1742-2051journal_volume
12pub_type
杂志文章abstract::Gene regulatory networks constitute the first layer of the cellular computation for cell adaptation and surveillance. In these webs, a set of causal relations is built up from thousands of interactions between transcription factors and their target genes. The large size of these webs and their entangled nature make it...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/B904960f
更新日期:2009-12-01 00:00:00
abstract::Microbial long chain alcohols and alkanes are renewable biofuels that could one day replace petroleum-derived fuels. Here we report a novel pathway for high efficiency production of these products in Escherichia coli strain BL21(DE3). We first identified the acyl-ACP reductase/aldehyde deformylase combinations with th...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00268k
更新日期:2015-09-01 00:00:00
abstract::The pro-inflammatory cytokines interleukin 1 (IL-1) and 6 (IL-6) are crucially involved in the regulation of a multitude of physiological processes, in particular coordinating the immune response upon bacterial infection and tissue injury. Both interleukins induce complex signalling cascades and trigger the production...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05261f
更新日期:2011-12-01 00:00:00
abstract::The transforming growth factor beta (TGF-β) signaling pathway plays myriad roles in development and disease. TGF-β isoforms initiate signaling by organizing their cell surface receptors TβRI and TβRII. Exploration and exploitation of the versatility of TGF-β signaling requires an enhanced understanding of structure-fu...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00115e
更新日期:2010-12-01 00:00:00
abstract::Sequence specific binding of DNA and RNA is of fundamental importance in the regulation of cellular gene expression. Because of their modular structure repeat domain proteins are particularly well suited for these processes and have been widely adopted throughout evolution. Detailed biochemical and structural data has...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c2mb05392f
更新日期:2012-03-01 00:00:00
abstract::Fluorescent molecules that specifically target amyloid structures are highly desirable for amyloid research. Herein, we show a dimeric design of thioflavin T improves its binding affinity to Abeta amyloid by up to 70 fold, while not sacrificing the specificity and the "light-up" feature upon amyloid binding. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c005255h
更新日期:2010-10-01 00:00:00
abstract::Individuals infected with the human immunodeficiency virus (HIV) often suffer from concomitant metabolic complications. Treatment with antiretroviral therapy has also been shown to alter the metabolism of patients. Although chemometric analysis of nuclear magnetic resonance (NMR) spectra of human sera can distinguish ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00347k
更新日期:2014-11-01 00:00:00
abstract::We demonstrate the expected preference of an immobilised oligosaccharide Man(9)(GlcNAc)(2) upon a 96-well photochemical array, for its known receptor, the cell-surface lectin Dendritic Cell-Specific ICAM3 Grabbing Nonintegrin (DC-SIGN) when compared to immobilised competing monosaccharides. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00118j
更新日期:2011-01-01 00:00:00
abstract::Recently, the development of Src/Abl (c-Src/Bcr-Abl tyrosine kinases) dual inhibitors has attracted considerable attention from the research community for treatment of malignancies. In order to explore the different structural features impacting the Src and Abl inhibitory activities of N(9)-arenethenyl purines and to ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00350k
更新日期:2015-02-01 00:00:00
abstract::Regulatory networks are able to process complex signals and respond appropriately to the cellular context. Thus, an increasing effort by systems biology researchers is being focused on understanding which interactions are responsible for a given functional response. When translated into specific mathematical models, h...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25243k
更新日期:2013-02-02 00:00:00
abstract::Secretagogin is a hexa EF-hand Ca(2+)-binding protein expressed in neuroendocrine, pancreatic endocrine and retinal cells. The protein has been noted for its expression in specific neuronal subtypes in the support of hierarchical organizing principles in the mammalian brain. Secretagogin has previously been found to i...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00349b
更新日期:2011-07-01 00:00:00
abstract::Breast cancer has been reported as one of the most frequently diagnosed malignant diseases and the leading cause of cancer death in women all around the world. Furthermore, this complicated cancer is divided into multiple subtypes which present different clinical symptoms and need correspondingly directed therapy. We ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00653a
更新日期:2017-05-02 00:00:00
abstract::Despite many prior studies on microbial response to oxidative stress, our understanding of microbial tolerance against oxidative stress is currently limited to aerobic conditions, and few engineering strategies have been devised to resolve toxicity issues of oxidative stress under anaerobic conditions. Since biologica...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25259g
更新日期:2013-02-02 00:00:00
abstract::Genome-wide RNA interference screens have greatly facilitated the identification of essential host factors (EHFs) for viral infections, whose knockdown effects significantly influence virus replication but not host cell viability. However, little has been done to link EHFs with another important host factor type, i.e....
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00309a
更新日期:2015-09-01 00:00:00
abstract::Thyroid carcinoma is a common endocrine malignancy worldwide, accounting for approximately 1% of all diagnosed cancers and about 91.5% of the malignancies of head and neck. However, differentiating malignant thyroid nodules from benign ones remains a diagnostic challenge. Thus, novel molecular markers that enable non-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05029j
更新日期:2011-09-01 00:00:00
abstract::In the present study, we focused on the expression and biological functions of TMEM45B in pancreatic cancer tissues and cell lines. Real-time PCR and Western blotting were used to examine the expression levels of TMEM45B in pancreatic cancer tissues and cell lines. The functions of TMEM45B were evaluated using CCK-8, ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00203j
更新日期:2016-05-24 00:00:00
abstract::Undoubtedly, metabonomics can reveal the comprehensive efficacies of traditional Chinese medicine (TCM) formulae and its complex mechanism at the molecular biological level. In this study, an attempt was made to address the pretreatment effect of a TCM formula. In this case, as a critical point, we should first know h...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00529e
更新日期:2015-02-01 00:00:00
abstract::To faithfully execute diverse biological programs all cells need to access and distribute their genomes in a highly organized way. In the nucleus of eukaryotic cells DNA is packed with histone proteins into chromatin. The originating nucleo-protein complex is the regulatory platform for all genetic processes. Of these...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c2mb25328c
更新日期:2013-02-02 00:00:00
abstract::Comparative analysis of the complete genome sequences from a variety of poorly studied organisms aims at predicting ecological and behavioral properties of these organisms and helping in characterizing their habitats. This task requires finding appropriate descriptors that could be correlated with the core traits of e...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b908047c
更新日期:2010-04-01 00:00:00
abstract::Regular physical activity is associated with a reduced risk of coronary heart disease, as it probably modifies the balance between free-radical generation and antioxidant activity. On the other hand, however, acute physical activity increases oxygen uptake and leads to a temporary imbalance between the production of r...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00106f
更新日期:2011-03-01 00:00:00
abstract::Major progress in the study of RNase P has resulted from crystallography of bacterial catalytic subunits and the discovery of catalytic activity in eukaryotes. Several new substrates have also been identified, primarily in bacteria but also in yeast. Our current world should be called the "RNA-protein world" rather th...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b707850c
更新日期:2007-09-01 00:00:00
abstract::Understanding the molecular mechanism that underlies the differentiation of neural stem cells (NSCs) is vital to develop regenerative medicines for neurological disorders. In our previous work, Rho-GDI-γ was found to be able to prompt neuronal differentiation when it was down regulated. However, it is unclear how Rho-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25147g
更新日期:2012-11-01 00:00:00
abstract::Understanding the complexity of cancer biology requires extensive information about the cancer proteome over the course of the disease. The recent advances in mass spectrometry-based proteomics technologies have led to the accumulation of an incredible amount of such proteomic information. This information allows us t...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c6mb00639f
更新日期:2016-12-20 00:00:00
abstract::Here we present a novel statistical methodology that allows us to analyze gene expression data that have been collected from a number of different cases or conditions in a unified framework. Using a Bayesian nonparametric framework we develop a hierarchical model wherein genes can maintain a shared set of interactions...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25497f
更新日期:2013-07-01 00:00:00
abstract::It is now widely accepted that microRNAs (miRNAs or miRs) along with transcription factors (TFs) weave a complex inter-regulatory network within the cell that is responsible for the combinatorial regulation of gene expression. Recently we have shown that miRNAs and TFs that form network clusters are also associated wi...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25518b
更新日期:2013-06-01 00:00:00
abstract::Large combinatorial libraries of macrocyclic peptides are a useful source of bioactive compounds. However, peptides are not generally cell permeable, so there is great interest in the development of methods to create large libraries of modified peptides. In particular, N-alkylation of peptides is known to improve thei...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00308c
更新日期:2015-10-01 00:00:00
abstract::Protein tyrosine phosphatases (PTPs) constitute a large family of enzymes that play key roles in cell signaling. Deregulation of PTP activity results in aberrant tyrosine phosphorylation, which has been linked to the etiology of several human diseases, including cancer. Since phosphate removal by the PTPs can both enh...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b700704n
更新日期:2007-05-01 00:00:00
abstract::Disordered regions within proteins have increasingly been associated with various cellular functions. Identifying the specific roles played by disorder in these functions has proved difficult. However, the development of reliable prediction algorithms has expanded the study of disorder from a few anecdotal examples to...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c1mb05235g
更新日期:2012-01-01 00:00:00
abstract::Single molecule observation of fluorescence resonance energy transfer can be used to provide insight into the structure and dynamics of proteins. Using a straightforward triple-colour labelling strategy, we present a measurement and analysis scheme that can simultaneously study multiple regions within single intrinsic...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25135c
更新日期:2012-10-01 00:00:00
abstract::Ca2+ transport across the sarco/endoplasmic reticulum (SR) plays an essential role in intracellular Ca2+ homeostasis, signalling, cell differentiation and muscle contractility. During SR Ca2+ uptake and release, proton fluxes are required to balance the charge deficit generated by the exchange of Ca2+ and other ions a...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c7mb00065k
更新日期:2017-03-28 00:00:00