Abstract:
:RNA interference (RNAi) techniques hold forth great promise for therapeutic silencing of deleterious genes. However, clinical applications of RNAi require the development of safe and efficient methods for intracellular delivery of small interfering RNA (siRNA) oligonucleotides specific to targeted genes. We describe the use of a lipitoid, a cationic oligopeptoid-phospholipid conjugate, for non-viral transfection of synthetic siRNA oligos in cell culture. This peptidomimetic delivery vehicle allows for efficient siRNA transfection in a variety of human cell lines with negligible toxicity and promotes extensive downregulation of the targeted genes at both the protein and the mRNA level. We compare the lipitoid reagent to a standard commercial transfection reagent. The lipitoid is highly efficient even in primary IMR-90 human lung fibroblasts in which other commercial reagents are typically ineffective.
journal_name
Mol Biosystjournal_title
Molecular bioSystemsauthors
Utku Y,Dehan E,Ouerfelli O,Piano F,Zuckermann RN,Pagano M,Kirshenbaum Kdoi
10.1039/b603229jsubject
Has Abstractpub_date
2006-06-01 00:00:00pages
312-7issue
6-7eissn
1742-206Xissn
1742-2051journal_volume
2pub_type
杂志文章abstract::There are clearly many different philosophies associated with adapting fragment screening into mainstream Drug Discovery Lead Generation strategies. Scientists at Astex, for instance, focus entirely on strategies involving use of X-ray crystallography and NMR. However, AstraZeneca uses a number of different fragment s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b610069b
更新日期:2006-09-01 00:00:00
abstract::To improve the performance of nanostructured calcium carbonate in gene delivery, a hydrophilic polysaccharide, alginate, was added to calcium carbonate co-precipitation systems to form alginate/CaCO(3)/DNA nanoparticles. The size and ζ-potential of the nanoparticles were measured by a zetasizer. Due to the existence o...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c1mb05337j
更新日期:2012-03-01 00:00:00
abstract::Stochastic fluctuations (noise) in gene expression can cause members of otherwise genetically identical populations to display drastically different phenotypes. An understanding of the sources of noise and the strategies cells employ to function reliably despite noise is proving to be increasingly important in describ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b801245h
更新日期:2008-07-01 00:00:00
abstract::Glucocorticoids (GCs) have been proved to be an important pathogenic factor of some neuropsychiatric disorders. Usually, a classical injury model based on corticosterone-induced cytotoxicity of differentiated rat pheochromocytoma (PC12) cells was used to stimulate the state of GC damage of hippocampal neurons and inve...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00642b
更新日期:2016-03-01 00:00:00
abstract::Drug promiscuity is one of the key issues in current drug development. Many famous drugs have turned out to behave unexpectedly due to their propensity to bind to multiple targets. One of the primary reasons for this promiscuity is that drugs bind to multiple distinctive target environments, a feature that we call mul...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b901356c
更新日期:2009-08-01 00:00:00
abstract::A metabolomic study was performed to investigate the biochemical profiles of livers from male and female zebrafish (Danio rerio), using a multiple platform approach, incorporating 1H NMR, GC/MS and LC/MS. The reproducibility and reliability of the three methods were validated prior to the assays. Major biomolecules de...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b811850g
更新日期:2009-03-01 00:00:00
abstract::Recently circular RNA (circularRNA) has been discovered as an increasingly important type of long non-coding RNA (lncRNA), playing an important role in gene regulation, such as functioning as miRNA sponges. So it is very promising to identify circularRNA transcripts from de novo assembled transcripts obtained by high-...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00214a
更新日期:2015-08-01 00:00:00
abstract::Bacterial resistance to β-lactam antibiotics poses a serious threat to human health. Penicillin binding proteins (PBPs) and β-lactamases are involved in both antibacterial activity and mediation of β-lactam antibiotic resistance. The two major reasons for resistance to β-lactams include: (i) pathogenic bacteria expres...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70537d
更新日期:2014-04-01 00:00:00
abstract::Estrogen positive breast cancer is a dreadful disease in women worldwide. The human estrogen receptor-α (ERα) pathway plays a critical role in estrogenic signaling and targeting ERα in breast cancer treatment. The key role of Lemur tyrosine kinase-3 (LMTK3) in regulation of ERα has been identified and it is found to b...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00063c
更新日期:2014-05-01 00:00:00
abstract::Fluorescent molecules that specifically target amyloid structures are highly desirable for amyloid research. Herein, we show a dimeric design of thioflavin T improves its binding affinity to Abeta amyloid by up to 70 fold, while not sacrificing the specificity and the "light-up" feature upon amyloid binding. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c005255h
更新日期:2010-10-01 00:00:00
abstract::The molecular segmentation clock is a complex regulatory network that governs the periodic somite segmentation in vertebrate embryos. Underlying the rhythm of the segmentation clock is a single-cell level pace-making circuit, where inevitable molecular noise and time delay impose normal operating constraints to the pa...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70046a
更新日期:2013-06-01 00:00:00
abstract::Integrative pharmacology has been used to identify the key active constituents (KACs) of Buchang Naoxintong capsules (BNCs), a traditional Chinese medical preparation; this approach involves the evaluation of the content profiles and drug-like properties of the BNC constituents and development of an ingredient-target ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00460h
更新日期:2016-01-01 00:00:00
abstract::Matrix metalloproteinases (MMPs) consist of a class of proteins required for normal tissue function. Their over expression is associated with many disease states and hence the interest in MMPs as drug targets. Almost all MMP inhibitors have been reported to fail in clinical trials due to lack of specificity. Zinc in t...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00003c
更新日期:2015-04-01 00:00:00
abstract::The crystal structure of the versatile CYP109B1 enzyme from Bacillus subtilis has been solved at 1.8 Å resolution. This is the first structure of an enzyme from this CYP family, whose members are prevalent across diverse species of bacteria. In the crystal structure the enzyme has an open conformation with an access c...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c4mb00665h
更新日期:2015-03-01 00:00:00
abstract::Biological networks are effective tools for studying molecular interactions. Modular structure, in which genes or proteins may tend to be associated with functional modules or protein complexes, is a remarkable feature of biological networks. Mining modular structure from biological networks enables us to focus on a s...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00617e
更新日期:2016-11-15 00:00:00
abstract::We recently reported a chemical genetic method for generating bivalent inhibitors of protein kinases. This method relies on the use of the DNA repair enzyme O(6)-alkylguanine-DNA alkyltransferase (AGT) to display an ATP-competitive inhibitor and a ligand that targets a secondary binding domain. With this method potent...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c0mb00108b
更新日期:2011-02-01 00:00:00
abstract::Protein-adsorptive properties are a key feature of membranes used for haemodialysis treatment. Protein adsorption is vital to the biocompatibility of a membrane material and influences membrane's performance. The object of the present study is to investigate membrane biocompatibility by correlating the adsorbed proteo...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb05393d
更新日期:2012-04-01 00:00:00
abstract::Production of biofuels has been one of the promising efforts in biotechnology in the past few decades. The perspective of these efforts can be reduction of increasing demands for fossil fuels and consequently reducing environmental pollution. Nonetheless, most previous approaches did not succeed in obviating many big ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00119j
更新日期:2016-07-19 00:00:00
abstract::The zebrafish Danio rerio is a model vertebrate organism for understanding biological mechanisms. Recent studies have explored using zebrafish as a model for lipid-related diseases, for in vivo fish bioassays, and for embryonic toxicity experiments. Mass spectrometry (MS) and MS imaging are established tools for lipid...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c6mb00168h
更新日期:2016-06-01 00:00:00
abstract::All trans-retinoic acid (ATRA) is widely used to direct the differentiation of cultured stem cells. When exposed to the pluripotent human embryonal carcinoma (EC) stem cell line, TERA2.cl.SP12, ATRA induces ectoderm differentiation and the formation of neuronal cell types. We report in this study that novel polyene ch...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb70273a
更新日期:2013-12-01 00:00:00
abstract::Structural insight into DNA quadruplex structures formed by oligodeoxyribonucleotides 3'TG5'-5'GGGT3' (QS55) and 5'TG3'-3'GGGT5' (QS33) is presented. NMR analysis reveals that QS33 forms a parallel-like four-fold symmetric quadruplex, while QS55 possesses a two-fold symmetry and is characterized by a tetrameric antipa...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b718778e
更新日期:2008-05-01 00:00:00
abstract::It is not surprising that a large number of diseases related to amyloid fibril depositions are formed in various organs. Therefore, it is necessary to understand the transformation of native proteins into amyloid fibrils in order to clarify which key elements of this process determine the pathway of protein misfolding...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c5mb00273g
更新日期:2015-08-01 00:00:00
abstract::Here we present a novel statistical methodology that allows us to analyze gene expression data that have been collected from a number of different cases or conditions in a unified framework. Using a Bayesian nonparametric framework we develop a hierarchical model wherein genes can maintain a shared set of interactions...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25497f
更新日期:2013-07-01 00:00:00
abstract::Cell penetrating peptides (CPPs), TATp, in particular, has been used widely for intracellular delivery of various agents ranging from small molecules to proteins, peptides, range of pharmaceutical nanocarriers and imaging agents. This review highlights the mechanisms of CPP-mediated delivery and summarizes numerous ex...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/b916297f
更新日期:2010-04-01 00:00:00
abstract::To faithfully execute diverse biological programs all cells need to access and distribute their genomes in a highly organized way. In the nucleus of eukaryotic cells DNA is packed with histone proteins into chromatin. The originating nucleo-protein complex is the regulatory platform for all genetic processes. Of these...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c2mb25328c
更新日期:2013-02-02 00:00:00
abstract::Homogeneous oligodeoxyguanylates incorporating small-molecule intercalator pyrene moieties in a 1,5 relationship form various structures of dimer, trimer, tetramer and internal hairpin form that are yellowish in color because of the intermolecular interactions between the pyrene units. ...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b926677a
更新日期:2010-06-01 00:00:00
abstract::Comparative analysis of the complete genome sequences from a variety of poorly studied organisms aims at predicting ecological and behavioral properties of these organisms and helping in characterizing their habitats. This task requires finding appropriate descriptors that could be correlated with the core traits of e...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/b908047c
更新日期:2010-04-01 00:00:00
abstract::This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c2mb25470k
更新日期:2013-05-01 00:00:00
abstract::Oxygen dependent modulation of red blood cell metabolism is a long investigated issue. However, the recent introduction of novel mass spectrometry-based approaches lends itself to implement our understanding of the effects of red blood cell prolonged exposure to anaerobiosis. Indeed, most of the studies conducted so f...
journal_title:Molecular bioSystems
pub_type: 杂志文章
doi:10.1039/c3mb25575a
更新日期:2013-06-01 00:00:00
abstract::Understanding the complexity of cancer biology requires extensive information about the cancer proteome over the course of the disease. The recent advances in mass spectrometry-based proteomics technologies have led to the accumulation of an incredible amount of such proteomic information. This information allows us t...
journal_title:Molecular bioSystems
pub_type: 杂志文章,评审
doi:10.1039/c6mb00639f
更新日期:2016-12-20 00:00:00