Abstract：
:Glutathione (GSH) is an important bioactive tripeptide and widely used in food, medicine and other industries. Recently, the bifunctional glutathione synthetase, GshF, has been applied for efficient GSH production under inducible promoter. In this study, the constitutive expression of GshF from Streptococcus sanguinis (GshFss) was investigated under four different constitutive promoters. Based on previous study, five genes in Escherichia coli JM109 were deleted to eliminate the degradation of precursors and GSH. The effects of gene knockout on the constitutive expression of GshFss and GSH production were evaluated by whole cell catalysis. Finally, the engineered strain JM03Pdel1 produced 24 mM glutathione with addition of 30 mM precursors in 5-L bioreactor fed-batch fermentation. The yield of GSH based on cysteine in JM03Pdel1was reached 80% without any inducer, which was improved by 17.3% than that in the control strain.
journal_name
J Biotechnoljournal_title
Journal of biotechnologyauthors
Cui X,Wan J,Zhang X,Wu H,Li Z,Ye Qdoi
10.1016/j.jbiotec.2018.11.001subject
Has Abstractpub_date
2019-01-10 00:00:00pages
39-45eissn
0168-1656issn
1873-4863pii
S0168-1656(18)30676-Xjournal_volume
289pub_type
杂志文章abstract：:The adsorption behaviors of two glucanases, TvEG and TrCel7A, on three lignins were investigated. Three lignins were isolated from raw aspen and its pretreated solid residue. The isolated lignins were labeled as Asp-MWL, DA-MWL (pretreated by dilute acid), and GL-MWL (pretreated by green liquor), respectively. The sur...
journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
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abstract：:The present study aims to valorize chestnut burrs, an important lignocellulosic waste, through a biorefinery concept. A solid residue rich in glucan (41.36 ± 0.59 %) and lignin (39.06 ± 0.01 %) obtained from a previous process of pre-hydrolysis was subjected to four treatments with water or NaOH to enhance enzymatic h...
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abstract：:Water shortage necessitated South Indian sago factory owners, extracting starch out of cassava tubers, to install biogas plants where a starch utilizing microbial community multiplies and reduces the biological oxygen demand (BOD) of the waste waters by presently about 30%. The purification efficiency of sago factory ...
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doi：10.1016/j.jbiotec.2012.11.013
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abstract：:Lactulose and galacto-oligosaccharides (GOS) are well recognized prebiotics derived from lactose. In the synthesis of lactulose with β-galactosidases GOS are also produced, but the ratio of lactulose and GOS in the product can be tuned at will, depending on the operation conditions, so to obtain an optimal product dis...
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pub_type： 杂志文章
doi：10.1016/0168-1656(94)90088-4
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doi：10.1016/j.jbiotec.2005.06.028
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pub_type： 杂志文章
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2005.08.014
更新日期：2006-02-24 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2006.01.025
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pub_type： 杂志文章,评审
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更新日期：2002-02-01 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2006.03.025
更新日期：2006-09-18 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/s0168-1656(97)00150-8
更新日期：1997-01-03 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章,评审
doi：10.1016/0168-1656(95)00090-9
更新日期：1996-01-26 00:00:00
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journal_title：Journal of biotechnology
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doi：10.1016/s0168-1656(01)00244-9
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2014-01-20 00:00:00
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2015.11.023
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journal_title：Journal of biotechnology
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journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2005.12.009
更新日期：2006-04-10 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2012-08-31 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2015-11-20 00:00:00
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journal_title：Journal of biotechnology
pub_type： 杂志文章
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更新日期：2013-06-20 00:00:00
abstract：:NeuroD2, one of the neurospecific basic helix-loop-helix transcription factors, has the ability to induce neural differentiation in undifferentiated cells. In this paper, we show that transduction of NeuroD2 protein induced mouse neuroblastoma cell line N1E-115 into neural differentiation. NeuroD2 has two basic-rich d...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/j.jbiotec.2006.04.021
更新日期：2006-11-01 00:00:00
abstract：:The factors which limit the proliferation of eukaryotic cells in vitro are still not well known. Ammonia is believed to be toxic for mammalian cell proliferation and secretion. We have tried two approaches to reducing the ammonia in the medium. We first limited the ammonia produced by the cells by replacing glutamine ...
journal_title：Journal of biotechnology
pub_type： 杂志文章
doi：10.1016/0168-1656(94)00142-y
更新日期：1995-02-21 00:00:00
