Abstract：
:The effect of three lysosomotropic compounds, chloroquine, monensin and NH4Cl, on the replication of foot-and-mouth disease virus (FMDV) type A12 was studied. Viral replication was almost totally inhibited by 0.5 mM chloroquine, 50 microM monensin, or 25 mM NH4Cl. Monensin and NH4Cl affected replication when added either before or within the first hour of infection. Chloroquine, however, still inhibited viral replication when added up to 2.5 h after infection. Assays of binding of radiolabeled virus to cells showed that these compounds had no effect on viral adsorption. Neither monensin nor NH4Cl had any significant effect on cellular protein synthesis, but there was no evidence of viral protein synthesis in cells infected in the presence of these compounds. In contrast, chloroquine inhibited both cellular and viral protein synthesis. Eclipse assays, performed in the presence of the compounds, showed that while chloroquine and NH4Cl had little effect on cell-induced degradation of incoming virions to 12 S protein subunits, monensin inhibited this reaction. The replication of representative members of all seven serotypes of FMDV was inhibited by monensin although some types were less sensitive to the compound than others. These results are consistent with a model which postulates that viral eclipse is the result of acidification of endocytic vesicles which degrade entrapped virions to 12 S protein subunits resulting in the release of genome RNA.
journal_name
Virus Resjournal_title
Virus researchauthors
Baxt Bdoi
10.1016/0168-1702(87)90032-3subject
Has Abstractpub_date
1987-05-01 00:00:00pages
257-71issue
3eissn
0168-1702issn
1872-7492pii
0168-1702(87)90032-3journal_volume
7pub_type
杂志文章相关文献
VIRUS RESEARCH文献大全abstract：:The complete nucleotide sequence (2209 nucleotides) of the RNA 3 of the cucumber mosaic virus strain Trk7 was determined. Trk7-CMV possesses two open reading frames which encode the 3a protein (279 amino acids) and the coat protein (218 amino acids). Sequence analysis proved the earlier biological and serological clas...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(94)90030-2
更新日期：1994-03-01 00:00:00
abstract：:Echovirus 18 (EV18) is one of the echovirus serotypes associated with human diseases and in particular aseptic meningitis. To facilitate studies of the molecular epidemiology of EV18 and the evolution of enteroviruses in general, the complete nucleotide (nt) sequence was determined for the echovirus 18 prototype strai...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00019-9
更新日期：2002-04-23 00:00:00
abstract：:This paper summarizes the progress in the search for hantaviruses and hantavirus infections in Africa. After having collected molecular evidence of an indigenous African hantavirus in 2006, an intensive investigation for new hantaviruses has been started in small mammals. Various novel hantaviruses have been molecular...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2013.12.039
更新日期：2014-07-17 00:00:00
abstract：:Bovine herpesvirus-1 infected cell protein 27 (BICP27) was detected predominantly in the nucleolus. The open reading frame of BICP27 was fused with the enhanced yellow fluorescent protein (EYFP) gene to investigate its subcellular localization in live cells and BICP27 was able to direct monomeric, dimeric or trimeric ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.07.024
更新日期：2009-11-01 00:00:00
abstract：:Serological parameters intend to describe antibody response to influenza vaccine in a population. However, there is uncertainty about the mathematical appropriateness and the biological or clinical meaning of conventionally used parameters. Theoretical considerations and exploration of a data-set of 16 studies with an...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2004.02.024
更新日期：2004-07-01 00:00:00
abstract：:Japanese encephalitis virus (JEV)-specific RNAs (including 42S RNA) were synthesized in subcellular fractions prepared from infected C6/36 cells. This in vitro RNA synthesis essentially required Mg2+ and four ribonucleotides, and it was enhanced by K+. The amounts of RNA synthesized in vitro (in extracts from JEV-infe...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(89)90078-6
更新日期：1989-08-01 00:00:00
abstract：:In situ hybridization and virus titration were used to characterize early stages of rat virus (RV) infection of rat pups after oronasal inoculation. Results suggest that virus enters through the lung and that early viremia leads rapidly to pantropic infection. Cells derived from all three germ layers were infected wit...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(93)90085-2
更新日期：1993-04-01 00:00:00
abstract：:Singapore grouper iridovirus (SGIV) is a large double-stranded DNA virus that is a major threat to grouper aquaculture. The pathogenesis of SGIV is not well understood so far. Previous studies have revealed that ICP18, an immediate early protein encoded by SGIV ORF086R gene, promotes viral replication by regulating ce...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2020.198218
更新日期：2021-01-02 00:00:00
abstract：:The fidelity of poliovirus RNA-dependent RNA polymerase (3D(pol)) was determined using a system based on the fidelity of synthesis of the alpha-lac gene which codes for a subunit of beta-galactosidase. Synthesis products are screened for mutations by an alpha-complementation assay, in which the protein product from al...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(00)00256-2
更新日期：2001-04-01 00:00:00
abstract：:To determine the pathogenicity domain and to apply cross protection, Pepper mild mottle virus (PMMoV) point-mutations in the replicase (REP) gene between the methyl-transferase and helicase domains, and deletions truncating pseudoknots in the 3' non-coding region (NCR), were constructed. Some mutants substituting a si...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2005.11.004
更新日期：2006-06-01 00:00:00
abstract：:The entry of herpes simplex virus (HSV) into an oral epithelial cell line, primary normal human oral keratinocytes (NHOK) and gingival fibroblasts (GF) was examined. Infection of these cells by HSV-1 and HSV-2 was blocked by heparin. Further examination indicated that heparin reduced viral attachment but not penetrati...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(02)00055-2
更新日期：2002-06-01 00:00:00
abstract：:Polyomavirus VP1 has been shown to be modified by phosphorylation, sulfation, acetylation and hydroxylation. The major capsid protein VP1 is now shown to be modified by methylation. Addition of cycloheximide to infected cultures followed by addition of [3H-methyl]-L-methionine and subsequent immunoprecipitation, SDS-P...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)01266-4
更新日期：1996-02-01 00:00:00
abstract：:Partial nucleotide sequences of the haemagglutinin (H) gene of 18 measles viruses isolated in the Gambia during 1994 and 1995 show a high degree of homology (> 98.5%) when compared with each other. These sequences form a cluster distinct from measles viruses isolated from other geographic regions and from the sequence...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(96)01434-7
更新日期：1997-05-01 00:00:00
abstract：:Porcine deltacoronavirus (PDCoV), a newly discovered enteric coronavirus, is a causative agent of severe clinical diarrhea and intestinal pathological damage in piglets. As a first step toward understanding the effect of PDCoV on host cells, we elucidated mechanisms underlying the process of apoptotic cell death after...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2018.06.008
更新日期：2018-07-15 00:00:00
abstract：:Real-time and visual tracking of viral infection is crucial for elucidating the infectious and pathogenesis mechanisms. To track the virus successfully, an efficient labeling method is necessary. In this review, we first discuss the practical labeling techniques for virus tracking in live cells. We then describe the c...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2017.05.007
更新日期：2017-06-02 00:00:00
abstract：:Bovine herpesvirus-1 (BoHV-1) causes disease in cattle with varied clinical forms. In the U.S. there are two BoHV1 subtypes, BoHV-1.1 and BoHV-1.2b. Control programs in North America incorporate modified live (MLV) or killed (KV) viral vaccines. However, BoHV-1 strains continue to be isolated from diseased animals or ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.06.017
更新日期：2016-09-02 00:00:00
abstract：:Northern blot analysis revealed the presence of varicella-zoster virus (VZV) gene 27 transcripts in infected cells. The Sal I-G DNA fragment, located in the unique long segment of the VZV genome and containing overlapping genes 26 and 27, was analyzed in an in vitro transcription-translation system. Translation of RNA...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(88)90015-9
更新日期：1988-05-01 00:00:00
abstract：:Experimental infection studies with bluetongue virus (BTV) in the mammalian host have a history that stretches back to the late 18th century. Studies in a wide range of ruminant and camelid species as well as mice have been instrumental in understanding BTV transmission, bluetongue (BT) pathogenicity/pathogenesis, vir...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2013.12.044
更新日期：2014-03-01 00:00:00
abstract：:Fourteen isolates of bovine herpesvirus 1 (BHV-1) found representative of more than 100 isolates studied, were compared by restriction fragment pattern analyses and molecularly characterized. A number of evolutionary links between the variants originally associated with infectious bovine rhinotracheitis and the varian...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(96)01383-4
更新日期：1996-12-01 00:00:00
abstract：:In present study, we report the first complete genomic sequence of pig bovine viral diarrhea (BVD) virus, that of strain ZM-95, which is 12,220 nucleotides long and contains short 5' and 3' non-coding regions and one open reading frame encoding a large polyprotein with 28 potential N-glycosylation sites (Asn-X-Ser or ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2006.05.005
更新日期：2006-12-01 00:00:00
abstract：:DMV, dolphin morbillivirus, a paramyxovirus of uncertain origin recently emerged in Mediterranean dolphins. This study presents the complete nucleotide sequence of the hemagglutinin (H) gene including the gene boundaries. The single open reading frame of the DMV H gene encodes a protein of 604 residues which exhibits ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(95)01254-0
更新日期：1996-01-01 00:00:00
abstract：:Virus entry into host cells relies on interactions between viral and host structures including lipids, carbohydrates and proteins. Particularly, protein-protein interactions between viral surface proteins and host proteins as well as secondary host protein-protein interactions play a pivotal role in coordinating virus...
journal_title：Virus research
pub_type： 杂志文章,评审
doi：10.1016/j.virusres.2015.09.006
更新日期：2016-06-15 00:00:00
abstract：:Friesian calves were infected with one of three isolates of bovine viral diarrhoea virus (BVDV) and used to establish parameters for an in vitro model of BVDV-reactive T-cell responses in cattle. The study assessed virus clearance, seroconversion, maturation of lymphoproliferative responses (both during and following ...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/s0168-1702(00)00131-3
更新日期：2000-03-01 00:00:00
abstract：:Dromedary camel hepatitis E virus (DcHEV), a novel hepatitis E virus, has been identified in dromedary camels in Dubai, United Arab Emirates. The antigenicity, pathogenicity and epidemiology of this virus have been unclear. Here we first used a recombinant baculovirus expression system to express the 13 and 111 N-term...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2015.06.022
更新日期：2015-12-02 00:00:00
abstract：:Influenza virus rgVac1sub-P0 (H5N1) (rgVac1-P0), in which a pair of dibasic amino acid residues was introduced at the cleavage site of the HA of a reassortant of H5N2 and H7N1 viruses of duck origin, was low pathogenic in chickens. Vac1sub-P3 (H5N1) (Vac1-P3) was selected as a highly pathogenic avian influenza virus b...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2013.02.001
更新日期：2013-05-01 00:00:00
abstract：:A velogenic Newcastle disease virus (NDV) strain, designated as SRZ03, was isolated from an egg layer flock with NDV vaccine immunization failure in China in 2003. Recombination was found in the F gene of SRZ03. Complete genome sequences analysis indicated that the N-terminal of SRZ03 F gene originated from a genotype...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2007.10.001
更新日期：2008-02-01 00:00:00
abstract：:Plasmids containing the varicella zoster virus (VZV) open reading frames (ORFs) 61 and 62 were used in a transient co-transfection assay to test for trans-activation of the VZV and herpes simplex virus type 1 (HSV-1) thymidine kinase (tk) promoters. The trans-activating potential of the polypeptides encoded by these V...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/0168-1702(90)90013-2
更新日期：1990-01-01 00:00:00
abstract：:Theiler's murine encephalomyelitis virus is divided into two subgroups on the basis of their different biological activities. GDVII subgroup strains cause acute and fatal encephalomyelitis in mice, while TO or DA subgroup strains cause non-fatal polioencephalomyelitis in weanling mice followed by virus persistence and...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2009.11.004
更新日期：2010-02-01 00:00:00
abstract：:The entire genome of a mid-Atlantic raccoon strain rabies virus (RRV) isolated in Canada was sequenced; this is the second North American wildlife rabies virus isolate to be fully characterized. The overall organization and length of the genome was similar to that of other lyssaviruses. The nucleotide sequence identit...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2008.04.029
更新日期：2008-09-01 00:00:00
abstract：:During 2014-2015, 270 fecal samples were collected from non-diarrheic, captive and wild African green monkeys (AGMs) on the island of St. Kitts, Caribbean region. By RNA-PAGE, picobirnaviruses (PBVs) were detected in sixteen captive AGMs. By RT-PCR and sequencing of partial gene segment-2, PBVs in 15 of these 16 sampl...
journal_title：Virus research
pub_type： 杂志文章
doi：10.1016/j.virusres.2016.12.021
更新日期：2017-02-15 00:00:00
