Abstract：
:In this study we describe the generation and influences on in vitro and in vivo osteogenesis of photo-cured hyaluronic acid (HA) hydrogels loaded with growth and differentiation factor 5 (GDF-5). Prior to loading GDF-5, we characterized the release profiles from these hydrogels and tested their respective cell viability, differentiation and in vivo bone regeneration. The results from this testing indicated that GDF-5 was observed to release in a sustained manner from the HA hydrogels I-III. MTT and Live/Dead assays showed that the HA hydrogels I-III have good biocompatibility for use as scaffolds for bone tissue regeneration. In vitro cell tests showed a higher level of MC3T3-E1 cell proliferation and differentiation on HA hydrogels I-III than on HA hydrogel 0. Moreover, in vivo animal tests showed that the HA hydrogels I and III had a significant improvement on osteogenesis. Overall, our results suggest that the HA-based hydrogel is a good biomaterial to deliver osteogenic differentiation factors such as GDF-5, and GDF-5 can be useful as an effective alternative to aid new bone formation.
journal_name
Bonejournal_title
Boneauthors
Bae MS,Ohe JY,Lee JB,Heo DN,Byun W,Bae H,Kwon YD,Kwon IKdoi
10.1016/j.bone.2013.11.019subject
Has Abstractpub_date
2014-02-01 00:00:00pages
189-98eissn
8756-3282issn
1873-2763pii
S8756-3282(13)00481-Xjournal_volume
59pub_type
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