Abstract:
:Autologous marrow stromal cells have been proposed as an adjuvant in the treatment of bone defects and diseases. This will require the development of culture conditions that permit their rapid expansion ex vivo while retaining their potential for further differentiation. Fibroblast growth factor (FGF)-2 has been proposed as a candidate for the ex vivo expansion of cells with enhanced osteogenic potential, and we have explored this possibility further using cells obtained from a large cohort of adult human donors. Treatment with FGF-2 (0.001-2.5 ng/mL) had no detectable effect on colony formation, but markedly increased their proliferative potential and that of their immediate progeny, as shown by the increases in colony size and cell number. Based on the observed increase in the expression of the developmental markers STRO-1 and alkaline phosphatase (AP), a major target for the actions of FGF-2 appears to be the more primitive cells of the osteoblast lineage, and that, when added in combination with the synthetic glucocorticoid dexamethasone (Dx), it interacts positively to promote further cell maturation. The maintenance of adequate levels of ascorbate was shown to be a critical component in determining the nature of the effect of FGF-2 on AP expression. Variation in the response (predominantly in the magnitude and/or sensitivity) of the cultured cell populations to treatment with FGF-2 was apparent, but a preliminary analysis indicated that this was not due to differences in the age or gender of the donors used. The cultured cell populations were found to express multiple FGF receptors (FGFRs; 1-4) and the observed changes in the spectrum and abundance of FGFRs expressed in relation to that of STRO-1 and AP are consistent with their expression being developmentally regulated during the process of osteogenic differentiation. These results provide novel insights into the mechanism of action of FGF-2 on human cells of the osteoblast lineage and support the use of this factor, alone or in combination with Dx, for the rapid, ex vivo expansion of cell populations with enhanced osteogenic potential.
journal_name
Bonejournal_title
Boneauthors
Walsh S,Jefferiss C,Stewart K,Jordan GR,Screen J,Beresford JNdoi
10.1016/s8756-3282(00)00319-7subject
Has Abstractpub_date
2000-08-01 00:00:00pages
185-95issue
2eissn
8756-3282issn
1873-2763pii
S8756-3282(00)00319-7journal_volume
27pub_type
杂志文章相关文献
BONE文献大全abstract::Although low bone mineral density (BMD) is strongly associated with increased fracture risk, up to 50% of those who suffer fractures are not detected as high-risk patients by BMD testing. Thus, new approaches may improve identification of those at increased risk for fracture by in vivo assessment of altered bone tissu...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.03.021
更新日期:2018-01-01 00:00:00
abstract::One year of treatment with bisphosphonates at 5x the dose used for post-menopausal osteoporosis significantly increases failure load and microdamage, and decreases toughness at multiple skeletal sites in intact female beagles. The goal of this study was to determine if similar changes occur with doses equivalent to th...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2006.04.028
更新日期:2006-10-01 00:00:00
abstract::The hips are frequently involved in inheritable diseases which affect the bones. The clinical and radiological presentation of these diseases may be very similar to common hip disorders as developmental dysplasia of the hip, osteoarthritis and avascular necrosis, so the diagnosis may be easily overlooked and treatment...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2020.115729
更新日期:2021-02-01 00:00:00
abstract::We proposed a novel surface modification for an artificial hip joint stem from the viewpoint of maintenance and establishment of appropriate bone function and microstructure, represented by the preferred alignment of biological apatite (BAp) and collagen (Col). Oriented grooves were introduced into the proximal medial...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.11.005
更新日期:2013-02-01 00:00:00
abstract::A serious side effect of glucocorticoid treatment is the development of osteoporosis. We have earlier shown that long-term glucocorticoid administration results in a decrease in longitudinal bone growth, cortical bone mass, and biomechanical strength, while growth hormone administration increases these parameters. The...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(96)00077-4
更新日期:1996-06-01 00:00:00
abstract:INTRODUCTION:In patients with beta thalassemia major, inadequate transfusion and chelation may compromise bone health and increase risk of fractures. The objective of this study was to describe the prevalence of fractures in Indian inadequately transfused and chelated children, adolescents and young adults with beta th...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115649
更新日期:2021-02-01 00:00:00
abstract::While increasing age, decreasing body mass index (BMI), and physical inactivity are known to increase hip fracture risk, whether these factors have similar effects on other common fractures is not well established. We used prospectively-collected data from a large cohort to examine the role of these factors on the ris...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.03.014
更新日期:2012-06-01 00:00:00
abstract::This study investigated the possible role in vitro of insulin-like growth factor I (IGF-I) as a mediator of the effects of growth hormone (GH) on osteoclastic resorption in an unfractioned rabbit bone cell model. After 4 days of rabbit bone cell culture, human GH (hGH) (50 ng/mL) and human IGF-I (hIGF-I) (50 ng/mL) si...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00224-x
更新日期:1998-01-01 00:00:00
abstract::The clinical development of tiludronate (tiludronic acid, disodium salt) for the treatment of Paget's disease of bone is now being complemented by another clinical trials program to investigate its use in osteoporosis. It is expected that osteoporosis will become a major indication for tiludronate, and this paper desc...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/8756-3282(95)00277-8
更新日期:1995-11-01 00:00:00
abstract:OBJECTIVE:To test the hypothesis that low birth weight twins have a higher risk of osteoportotic fracture in later life, we investigated the association between fetal IGF axis and type-1 collagen markers of bone turnover in monochorionic (MC) twins with or without discordant birth weight of >or=20%. METHODS:Maternal a...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.11.003
更新日期:2006-06-01 00:00:00
abstract::Twenty patients with mild Paget's disease of bone were given either 20 (10 patients) or 40 mg alendronate daily for 6 months. The 20-mg dose was well tolerated, but in 3 patients on 40 mg/d alendronate, the treatment was withdrawn after 3-5 months because of gastric and oesophageal disturbances. Urinary hydroxyproline...
journal_title:Bone
pub_type: 临床试验,杂志文章
doi:10.1016/8756-3282(94)90818-4
更新日期:1994-07-01 00:00:00
abstract::Percutaneous vertebroplasty consists of injecting polymethylmethacrylate cement into the cancellous bone of vertebral bodies for the treatment of various lesions of the spine, including osteoporotic compression fractures. Clinicians practicing vertebroplasty commonly alter the mixture of monomer-to-powder recommended ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(99)00129-5
更新日期:1999-08-01 00:00:00
abstract::Both bone density and quality are important determinants of bone strength. Bone quality is prescribed by matrix characteristic including collagen cross-linking and bone structural characteristics and is important in reinforcement of bone strength. We investigated the effects of alfacalcidol (ALF), a prodrug of calcitr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.12.008
更新日期:2010-04-01 00:00:00
abstract::Osteonecrosis of the jaw (ONJ) is now defined by the presence of exposed bone in the mouth, which fails to heal after appropriate intervention over a period of six or eight weeks. It is commonly precipitated by a tooth extraction in patients treated with zoledronate, pamidronate or a combination of these agents, for t...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2008.09.012
更新日期:2009-01-01 00:00:00
abstract::The potential of whole body vibration (WBV) to maintain or enhance musculoskeletal strength during ageing is of increasing interest, with both low and high magnitude WBV having been shown to maintain or increase bone mineral density (BMD) at the lumbar spine and femoral neck. The aim of this study was to determine how...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115802
更新日期:2021-03-01 00:00:00
abstract::MicroRNAs (miRNAs) are a class of short RNA molecules that mediate the regulation of gene activity through interactions with target mRNAs and subsequent silencing of gene expression. It has become increasingly clear the miRNAs regulate many diverse aspects of bone biology, including bone formation and bone resorption ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115757
更新日期:2021-02-01 00:00:00
abstract::Recent studies suggest that whole-body vibration (WBV) can improve measures of bone health for certain clinical conditions and ages. In the elderly, there also is particular interest in assessing the ability of physical interventions such as WBV to improve coordination, strength, and movement speed, which help prevent...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.07.014
更新日期:2010-10-01 00:00:00
abstract::In order to assess the ability of six potential bone graft substitutes to support the growth of human osteoblasts, these cells were grown in culture and then plated onto fragments of the six materials and cultured for a further period of 15 days. Tests to confirm the osteoblastic phenotype of the cells included spectr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(93)90089-s
更新日期:1993-07-01 00:00:00
abstract::Osteoclasts (OCs) attach to the extracellular matrix via specialized attachment structures called podosomes, which form a prominent F-actin-rich ring that is thought to correspond to the sealing zone of resorbing OCs. Calcitonin (CT), a 32-amino acid polypeptide, inhibits bone resorption by decreasing motility, induci...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.01.014
更新日期:2007-05-01 00:00:00
abstract:UNLABELLED:We evaluated the effect of glucocorticoids (GC) and growth hormone (GH) on cortical and cancellous bone turnover in adult rats using random vertical sections giving valid measurements of bone surfaces and bone formation parameters. GH administration could reverse GC-induced osteopenia and low bone turnover o...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.07.015
更新日期:2005-01-01 00:00:00
abstract::Biochemical bone turnover markers (BTMs) provide important information on the diagnosis, therapy and monitoring of metabolic bone diseases including osteoporosis. One goal of antiresorptive therapy in women is to decrease biochemical BTMs to the lower half of reference intervals for healthy pre-menopausal counterparts...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.07.017
更新日期:2010-10-01 00:00:00
abstract::Recently, the involvement of immune responses in metabolic bone disease and/or local bone destruction has received much attention. Cytotoxic T lymphocyte-associated antigen 4 (CTLA-4), a member of the immunoglobulin (Ig) superfamily, negatively regulates T cell activation. The deficiency of CTLA-4 induces profound ost...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.06.018
更新日期:2004-11-01 00:00:00
abstract::Although vitamin D supplementation is a fundamental part of osteoporosis treatment, many patients do not regularly take adequate amounts. A once-weekly (OW) alendronate (ALN) preparation that includes 2800 IU of vitamin D3 in a single combination tablet (ALN+D2800) is available for treating patients and ensuring intak...
journal_title:Bone
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.bone.2008.05.002
更新日期:2009-04-01 00:00:00
abstract::Chondrocyte proliferation and differentiation are crucial for endochondral ossification and strictly regulated by numerous signaling molecules and transcription factors, but the hierarchical regulatory network remains to be deciphered. The present study emphasized the interplay of Activin A, Foxa, Notch and Shh signal...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.03.036
更新日期:2019-06-01 00:00:00
abstract::There is increasing evidence that osteogenic cells are present not only in bone marrow (BM) but also in peripheral blood (PB). Since staining for alkaline phosphatase (AP) identifies osteoprogenitor cells in BM, we sought to further characterize BM versus PB hematopoietic lineage negative (lin-)/AP+ cells and to compa...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2010.03.018
更新日期:2010-07-01 00:00:00
abstract::Approximately 7.9 million fractures occur annually in the United States with 5-10% of these resulting in delayed or impaired healing. Nearly half of the trauma cost of $56 billion per year is used for the treatment of fractures. More importantly, fracture results in a substantial reduction in the quality of life. New ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.06.037
更新日期:2011-11-01 00:00:00
abstract::Estrogen and calcium deficiencies increase both bone resorption and formation, whereas immobilization mainly decreases bone formation. How these functionally different risk factors for bone loss interact in cancellous bone undergoing modeling or remodeling activity is not well understood. Mature (6-month-old) female r...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00070-7
更新日期:1997-07-01 00:00:00
abstract::Active vitamin D is a major therapeutic agent for bone disease. Although some studies have reported that vitamin D ameliorates bone disease related to diabetes, the mechanism remains unclear. Our study investigated the effect of the vitamin D receptor activator 22-oxacalcitriol (OCT) on bone disease in a rat model of ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.01.014
更新日期:2015-05-01 00:00:00
abstract:INTRODUCTION:Teriparatide is a bone formation agent that increases bone turnover and mass, resulting in an increase in bone strength and a decrease in fracture risk. METHODS:The primary purpose of this analysis was to evaluate the association between pretreatment bone turnover marker (BTM) concentrations and the absol...
journal_title:Bone
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/j.bone.2006.02.003
更新日期:2006-08-01 00:00:00
abstract::Saracatinib (AZD0530) is an orally active once-daily Src kinase inhibitor which modulates key signaling pathways in cancer cells. In a Phase I study in patients with advanced solid malignancies resistant to standard treatment we assessed the effect of saracatinib on bone turnover. Fifty-one patients were randomized in...
journal_title:Bone
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1016/j.bone.2011.12.017
更新日期:2012-04-01 00:00:00