Abstract:
:Glycosylation is the most common post-translational modification of serum proteins, and changes in the type and abundance of glycans in human serum have been correlated with a growing number of human diseases. While the glycosylation pattern of human serum is well studied, little is known about the profiles of other mammalian species. Here, we report detailed glycosylation profiling of canine serum by hydrophilic interaction chromatography-ultraperformance liquid chromatography (HILIC-UPLC) and mass spectrometry. The domestic dog (Canis familiaris) is a widely used model organism and of considerable interest for a large veterinary community. We found significant differences in the serum N-glycosylation profile of dogs compared to that of humans, such as a lower abundance of galactosylated and sialylated glycans. We also compare the N-glycan profile of canine serum to that of canine IgG - the most abundant serum glycoprotein. Our data will serve as a baseline reference for future studies when performing serum analyses of various health and disease states in dogs.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Behrens AJ,Duke RM,Petralia LM,Harvey DJ,Lehoux S,Magnelli PE,Taron CH,Foster JMdoi
10.1093/glycob/cwy070subject
Has Abstractpub_date
2018-11-01 00:00:00pages
825-831issue
11eissn
0959-6658issn
1460-2423pii
5076956journal_volume
28pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::There is increasing evidence that secretory fluids such as tears, saliva and milk play an important role in protecting the human body from infection via a washing mechanism involving glycan-mediated adhesion of potential pathogens to secretory glycoproteins. Interaction of sweat with bacteria is well established as th...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwv102
更新日期:2016-03-01 00:00:00
abstract::Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws119
更新日期:2012-12-01 00:00:00
abstract::Changes in mucin protein expression and in glycosylation are common features in pre-neoplastic lesions and cancer and are therefore used as cancer-associated markers. De novo expression of intestinal mucin MUC2 and cancer-associated sialyl-Tn antigen are frequently observed in intestinal metaplasia (IM) and gastric ca...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp161
更新日期:2010-02-01 00:00:00
abstract::Exciting discoveries in many diverse fields of hyaluronan (HA) biology over the last 40 years have centered around the ability of HA to bind cell surface HA receptors (e.g., CD44, Layilin, LYVE-1, HARE/Stab2 and RHAMM) and sometimes also to activate intracellular signal transduction pathways, frequently involving ERK1...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwx056
更新日期:2017-09-01 00:00:00
abstract::Heparin/heparan sulfates (H/HS) are ubiquitous biopolymers that interact with many proteins to induce a range of biological functions. Unfortunately, how these biopolymers recognize their preferred protein targets remain poorly understood. It is suggested that computational simulations offer attractive avenues but a n...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwaa015
更新日期:2020-07-16 00:00:00
abstract::Endo-alpha-D-mannosidase is a processing enzyme which in contrast to other glycosidases involved in the trimming of N-linked oligosaccharides of glycoproteins acts at an internal position by cleaving the linkage between the glucose-substituted mannose and the internal portion of the polymannose unit and thereby provid...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.4.579
更新日期:1997-06-01 00:00:00
abstract::Dendritic cell (DC) migration to secondary lymphoid organs is a critical step to properly exert its role in immunity and predominantly depends on the interaction of the chemokine receptor CCR7 with its ligands CCL21 and CCL19. Polysialic acid (PSA) has been recently reported to control CCL21-directed migration of matu...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq078
更新日期:2010-09-01 00:00:00
abstract::Muscle degenerative diseases such as Duchenne Muscular Dystrophy are incurable and treatment options are still restrained. Understanding the mechanisms and factors responsible for muscle degeneration and regeneration will facilitate the development of novel therapeutics. Several recent studies have demonstrated that G...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn079
更新日期:2008-11-01 00:00:00
abstract::Galectin-8 (gal-8) is a "tandem-repeat"-type galectin, containing two carbohydrate recognition domains connected by a linker peptide. gal-8 is expressed both in the cytoplasm and nucleus in vascular endothelial cells (ECs) from normal and tumor-associated blood vessels, and in lymphatic endothelial cells. Herein, we d...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwu054
更新日期:2014-10-01 00:00:00
abstract::A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh022
更新日期:2004-02-01 00:00:00
abstract::Lipid-A represents the ubiquitous, covalently bound hydrophobic component of bacterial lipopolysaccharides (endotoxins). Lipid-As isolated and characterized from rhizobial species have large variations in their backbone sugars, as well as in their hydroxy fatty acid substituents. The sugar backbones consist of either ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.6.535
更新日期:1992-12-01 00:00:00
abstract::We report the production of biologically active hyaluronan (HA) oligosaccharides labeled with the fluorophore 2-aminobenzoic acid (2AA). Oligosaccharides from 4 to 40 residues in length were purified to homogeneity by ion exchange chromatography using a logarithmic gradient. Molecular weight and purity characterizatio...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi008
更新日期:2005-03-01 00:00:00
abstract::In this work, focused on possible application of α-L-fucosidases from bacterial sources in the synthesis of α-L-fucosylated glycoconjugates, several nonpathogenic aerobic bacterial strains were screened for α-L-fucosidase activity. Among them Paenibacillus thiaminolyticus was confirmed as a potent producer of enzyme w...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt041
更新日期:2013-09-01 00:00:00
abstract::The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl057
更新日期:2007-01-01 00:00:00
abstract::Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonst...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.5.411
更新日期:1992-10-01 00:00:00
abstract::Spectrofluorimetry experiments using synthetic trisaccharides indicate that in compounds that display affinity for antithrombin III (AT-III), a unique trisaccharide sequence plays the key role in the early recognition, and the first step of AT-III activation. Added to previous observations, these new results suggest t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.3.323-e
更新日期:1997-04-01 00:00:00
abstract::Glycosyltransferases, usually residing within the intracellular secretory apparatus, also circulate in the blood. Many of these blood-borne glycosyltransferases are associated with pathological states, including malignancies and inflammatory conditions. Despite the potential for dynamic modifications of glycans on dis...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww108
更新日期:2017-01-01 00:00:00
abstract::Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydrate-binding activity at the cell surface. In contrast, galectins were isolated on the b...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwq097
更新日期:2010-10-01 00:00:00
abstract::Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of th...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt091
更新日期:2014-02-01 00:00:00
abstract::Escherichia coli K92 produces a capsular polysialic acid with alternating alpha2,8 alpha2,9 NeuNAc linkages. This polysaccharide is cross-reactive with the neuroinvasive pathogen Neisseria meningitidis Group C. The K92 polysialyltransferase (PST) catalyzes the synthesis of the polysialic acid with alternating linkages...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm032
更新日期:2007-07-01 00:00:00
abstract::Knowledge of the identity, synthesis and secretion of beta-galactoside-binding lectins by leukocytes is of importance because lactosaminoglycans present at the leukocyte cell surface may be physiologically significant lectin receptors that could mediate autocrine or paracrine functions and/or cell adhesion. This paper...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.4.285
更新日期:1992-08-01 00:00:00
abstract::Tarin, the Colocasia esculenta lectin from the superfamily of α-d-mannose-specific plant bulb lectins, is a tetramer of 47 kDa composed of two heterodimers. Each heterodimer possesses homologous monomers of ~11.9 (A chain) and ~12.7 (B chain) kDa. The structures of apo and carbohydrate-bound tarin were solved to 1.7 Å...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww083
更新日期:2017-01-01 00:00:00
abstract::Aspartic protease, widely used as a milk-coagulating agent in industrial cheese production, contains three potential N-glycosylation sites. In this study, we report the characterization of N-linked oligosaccharides on recombinant aspartic protease secreted from the methylotrophic yeast Pichia pastoris using a combinat...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.10.1037
更新日期:1999-10-01 00:00:00
abstract::Glycolipids are important components of almost all biological membranes. They possess unique properties that have only been incompletely characterized so far. The plant glycolipid digalactosyldiacylglycerol (DGDG) strongly influences the physical behavior of phospholipid model membranes in both the dry and hydrated st...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj001
更新日期:2005-11-01 00:00:00
abstract::Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj092
更新日期:2006-06-01 00:00:00
abstract::Cytotrophoblasts are the specialized epithelial cells of the placenta. During the first trimester, a subpopulation of chorionic villus cytotrophoblasts differentiates along an invasive pathway and penetrates the maternal endometrium, decidua and spiral arterioles. Cytotrophoblast invasiveness declines rapidly during t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.5.567
更新日期:1994-10-01 00:00:00
abstract::Protein N- and O-glycosylation are well known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is known to influence antigen binding and effector functions, respectively. To study associations between antibody glycosylation profiles and (patho) physiological...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwz048
更新日期:2020-03-20 00:00:00
abstract::Sialidases are widely distributed glycohydrolytic enzymes removing sialic acid residues from glycoconjugates. In mammals, several sialidases with different subcellular localizations and biochemical features have been described. NEU4, the most recently identified member of the human sialidase family, is found in two fo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp156
更新日期:2010-02-01 00:00:00
abstract::Phytohaemagglutinin (PHA) is the major lectin present in the seeds of the common bean, Phaseolus vulgaris, and PHA-L is the leucocyte-agglutinating form of this lectin. This tetrameric glycoprotein accumulates in the vacuoles of storage parenchyma cells. Based on amino acid sequence comparisons of legume lectins and t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.6.581
更新日期:1993-12-01 00:00:00
abstract::Endolysins are bacteriophage enzymes that lyse their bacterial host for phage progeny release. They commonly contain an N-terminal catalytic domain that hydrolyzes bacterial peptidoglycan (PG) and a C-terminal cell wall-binding domain (CBD) that confers enzyme localization to the PG substrate. Two endolysins, phage ly...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt019
更新日期:2013-07-01 00:00:00