Incorporation of orally applied (13)C-galactose into milk lactose and oligosaccharides.

abstract：:UDP-N-acetylglucosamine (UDP-GlcNAc) is a glucose metabolite with pivotal functions as a key substrate for the synthesis of glycoconjugates like hyaluronan, and as a metabolic sensor that controls cell functions through O-GlcNAc modification of intracellular proteins. However, little is known about the regulation of h...

journal_title：Glycobiology

authors： Oikari S,Makkonen K,Deen AJ,Tyni I,Kärnä R,Tammi RH,Tammi MI

更新日期：2016-07-01 00:00:00

abstract：:Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...

journal_title：Glycobiology

authors： Gerwig GJ,van Kuik JA,Leeflang BR,Kamerling JP,Vliegenthart JF,Karr CD,Jarroll EL

更新日期：2002-08-01 00:00:00

abstract：:Lectins are used as defense effector proteins against predators, parasites and pathogens by animal, plant and fungal innate defense systems. These proteins bind to specific glycoepitopes on the cell surfaces and thereby interfere with the proper cellular functions of the various antagonists. The exact cellular toxicit...

journal_title：Glycobiology

authors： Bleuler-Martinez S,Stutz K,Sieber R,Collot M,Mallet JM,Hengartner M,Schubert M,Varrot A,Künzler M

更新日期：2017-05-01 00:00:00

abstract：:P3 is a mouse monoclonal antibody (mAb) that binds to several NeuGc-containing gangliosides. It also reacts with antigens expressed in human breast tumors (Vázquez et al. (1995) Hybridoma , 14, 551-556). In this work, the binding specificity of P3 has been characterized in more detail using a panel of glycolipids that...

journal_title：Glycobiology

authors： Moreno E,Lanne B,Vázquez AM,Kawashima I,Tai T,Fernández LE,Karlsson KA,Angström J,Pérez R

更新日期：1998-07-01 00:00:00

abstract：:Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of th...

journal_title：Glycobiology

authors： Muindi KM,McCarthy PC,Wang T,Vionnet J,Battistel M,Jankowska E,Vann WF

更新日期：2014-02-01 00:00:00

abstract：:Differences in the fertilization behavior of Xenopus borealis from X. laevis and X. tropicalis suggest differences in the glycosylation of the egg jellies. To test this assumption, O-linked glycans were chemically released from the egg jelly coat glycoproteins of X. borealis. Over 50 major neutral glycans were observe...

journal_title：Glycobiology

authors： Li B,Russell SC,Zhang J,Hedrick JL,Lebrilla CB

更新日期：2011-07-01 00:00:00

abstract：:Epithelial cells in the lining of the intestines play critical roles in maintaining homeostasis while challenged by dynamic and sudden changes in luminal contents. Given the high density of glycosylation that encompasses their extracellular surface, environmental changes may lead to extensive reorganization of membran...

journal_title：Glycobiology

authors： Park D,Xu G,Barboza M,Shah IM,Wong M,Raybould H,Mills DA,Lebrilla CB

更新日期：2017-09-01 00:00:00

abstract：:In Campylobacter jejuni, the PglB oligosaccharyltransferase catalyzes the transfer of a heptasaccharide from a lipid donor to asparagine within the D/E-X1-N-X2-S/T sequon (X1,2 ≠ P) or releases this heptasaccharide as free oligosaccharides (fOS). Using available crystal structures and sequence alignments, we identifie...

journal_title：Glycobiology

authors： Barre Y,Nothaft H,Thomas C,Liu X,Li J,Ng KKS,Szymanski CM

更新日期：2017-10-01 00:00:00

abstract：:The deletion of the protein mannosyltransferase 1 gene (PMT1) of Saccharomyces cerevisiae results in viable cells. O-Mannosylation of proteins is reduced to about half of the value in comparison to wild-type cells. In order to distinguish between the the PMT1 gene product (= Pmt1p) and residual transferase activity, a...

journal_title：Glycobiology

authors： Gentzsch M,Strahl-Bolsinger S,Tanner W

更新日期：1995-02-01 00:00:00

abstract：:Polysialic acid (PSA) is thought to attenuate neural cell adhesion molecule (NCAM) adhesion, thereby facilitating neural cell migration and regeneration. Although the expression of PSA has been shown to correlate with the progression of certain tumors such as small cell lung carcinoma, there have been no studies to de...

journal_title：Glycobiology

authors： Suzuki M,Suzuki M,Nakayama J,Suzuki A,Angata K,Chen S,Sakai K,Hagihara K,Yamaguchi Y,Fukuda M

更新日期：2005-09-01 00:00:00

abstract：:The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...

journal_title：Glycobiology

authors： Kang NY,Kang SK,Lee YC,Choi HJ,Lee YS,Cho SY,Kim YS,Ko JH,Kim CH

更新日期：2006-05-01 00:00:00

abstract：:β-Trefoil lectins are galactose/N-acetyl galactosamine specific lectins, which are widely distributed across all kingdoms of life and are known to perform several important functions. However, there is no report available on the characterization of these lectins from protozoans. We have performed structural and biophy...

journal_title：Glycobiology

authors： Khan F,Kurre D,Suguna K

更新日期：2020-07-20 00:00:00

abstract：:The content of sulfated glycans having 6-O-sulfated GlcNAc residues alters in the course of colonic carcinogenesis. We previously characterized two GlcNAc 6-O-sulfotransferases (SulTs), SulT-a and -b, expressed in colonic normal tissues and adenocarcinomas [Seko et al. (2000) Glycobiology, 10, 919-929]. Levels of the ...

journal_title：Glycobiology

authors： Seko A,Nagata K,Yonezawa S,Yamashita K

更新日期：2002-06-01 00:00:00

abstract：:We have previously shown that varicella-zoster virus (VZV) and cytomegalovirus (CMV) infection of diploid human fibroblasts (HEL) results in neo-expression of Lewis antigens sialyl Lewis x (sLe(x)) and Lewis y (Le(y)), respectively, after transcriptional activation of different combinations of dormant human fucosyltra...

journal_title：Glycobiology

authors： Nyström K,Nordén R,Muylaert I,Elias P,Larson G,Olofsson S

更新日期：2009-08-01 00:00:00

abstract：:The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...

journal_title：Glycobiology

authors： De Castro C,Carannante A,Lanzetta R,Liparoti V,Molinaro A,Parrilli M

更新日期：2006-12-01 00:00:00

abstract：:The N-glycans have been removed by peptide-N-glycosidase F (PNGase F) from purified human non-secretory RNases derived from kidney, liver and spleen. The spleen RNase was purified by two procedures, one of which did not include the usual acid treatment step (0.25 M H2SO4, 45 min, 4 degrees C), to determine if acid tre...

journal_title：Glycobiology

authors： Lawrence CW,Little PA,Little BW,Glushka J,van Halbeek H,Alhadeff JA

更新日期：1993-06-01 00:00:00

abstract：:The polydispersity of hyaluronan (HA) presents challenges for analyzing its solution properties, such as the relationship between mass and particle size. The broad mass range of natural HA (≤50-fold) makes molecular characterization difficult and ambiguous compared to molecules with known molecular weights (e.g., prot...

journal_title：Glycobiology

authors： Weigel PH,Baggenstoss BA

更新日期：2017-09-01 00:00:00

abstract：:Apolipoprotein C-III (apoCIII) is a small glycoprotein with a single mucin-type core-1 oligosaccharide and is analyzed by isoelectric focusing (IEF) for the diagnosis of genetic defects in O-glycan biosynthesis such as congenital disorders of glycosylation. In the present study, mass spectrometry of apoCIII, after a s...

journal_title：Glycobiology

authors： Wada Y,Kadoya M,Okamoto N

更新日期：2012-08-01 00:00:00

abstract：:Endolysins are bacteriophage enzymes that lyse their bacterial host for phage progeny release. They commonly contain an N-terminal catalytic domain that hydrolyzes bacterial peptidoglycan (PG) and a C-terminal cell wall-binding domain (CBD) that confers enzyme localization to the PG substrate. Two endolysins, phage ly...

journal_title：Glycobiology

authors： Ganguly J,Low LY,Kamal N,Saile E,Forsberg LS,Gutierrez-Sanchez G,Hoffmaster AR,Liddington R,Quinn CP,Carlson RW,Kannenberg EL

更新日期：2013-07-01 00:00:00

abstract：:Gangliosides are able to bind to the epidermal growth factor receptor and inhibit its activation, but the mechanism of this inhibition is unknown. To address the role of receptor carbohydrates in facilitating interaction with gangliosides, we examined the ability of GM3 to bind the deglycosylated receptor and inhibit ...

journal_title：Glycobiology

authors： Wang XQ,Sun P,O'Gorman M,Tai T,Paller AS

更新日期：2001-07-01 00:00:00

abstract：:Hypertrophy is central to several heart diseases; however, not much is known about the role of glycosphingolipids (GSLs) in this phenotype. Since GSLs have been accorded several physiological functions, we sought to determine whether these compounds affect cardiac hypertrophy. By using a rat cardiomyoblast cell line, ...

journal_title：Glycobiology

authors： Mishra S,Chatterjee S

更新日期：2014-06-01 00:00:00

abstract：:Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...

journal_title：Glycobiology

authors： Guarino C,DeLisa MP

更新日期：2012-05-01 00:00:00

abstract：:Different classes of glycans are implicated as mediators of apical protein sorting in the secretory pathway of epithelial cells, but recent research indicates that sorting to the apical and basolateral surfaces may occur before completion of glycan synthesis. We have previously shown that a proteoglycan (PG) core prot...

journal_title：Glycobiology

authors： Moen A,Hafte TT,Tveit H,Egge-Jacobsen W,Prydz K

更新日期：2011-11-01 00:00:00

abstract：:In renal cell carcinoma (RCC), the presence of higher gangliosides correlates with systematic metastasis. Disialosyl globopentaosylceramide (DSGb5) was identified previously as one of the major gangliosides from RCC tissues. Siglec-7 (sialic acid-binding Ig-like lectin-7), expressed on natural killer (NK) cells as an ...

journal_title：Glycobiology

authors： Kawasaki Y,Ito A,Withers DA,Taima T,Kakoi N,Saito S,Arai Y

更新日期：2010-11-01 00:00:00

abstract：:Protein N- and O-glycosylation are well known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is known to influence antigen binding and effector functions, respectively. To study associations between antibody glycosylation profiles and (patho) physiological...

journal_title：Glycobiology

authors： de Haan N,Falck D,Wuhrer M

更新日期：2020-03-20 00:00:00

abstract：:The high-molecular-mass salivary mucin MG1, one of two major mucins produced by human salivary glands, plays an important role in oral health by coating the tooth surface and by acting as a bacterial receptor. Here this mucin was purified from the submandibular/sublingual saliva of a blood group O individual. The pres...

journal_title：Glycobiology

authors： Thomsson KA,Prakobphol A,Leffler H,Reddy MS,Levine MJ,Fisher SJ,Hansson GC

更新日期：2002-01-01 00:00:00

abstract：:The acid alpha-mannosidase of Trypanosoma cruzi is a broad-specificity hydrolase involved in the catabolism of glycoconjugates, presumably in the digestive vacuole. We have cloned the alpha-mannosidase gene from a T.cruzi epimastigote genomic library. The alpha-mannosidase gene was determined to be single copy by Sout...

journal_title：Glycobiology

authors： Vandersall-Nairn AS,Merkle RK,O'Brien K,Oeltmann TN,Moremen KW

更新日期：1998-12-01 00:00:00

abstract：:The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...

journal_title：Glycobiology

authors： Hofinger ES,Spickenreither M,Oschmann J,Bernhardt G,Rudolph R,Buschauer A

更新日期：2007-04-01 00:00:00

abstract：:Sulfated O-linked oligosaccharides from the sea urchin egg receptor have been shown to bind to acrosome-reacted sperm and to inhibit fertilization in a competitive bioassay. However, the inhibitory activity of these isolated chains was much lower than that of a recombinant protein representing a portion of the extrace...

journal_title：Glycobiology

authors： Dhume ST,Stears RL,Lennarz WJ

更新日期：1996-01-01 00:00:00

abstract：:Detailed structural and functional studies over the last decade have led to current recognition of the mycobacterial lipoarabinomannan (LAM) as a phosphatidylinositol anchored lipoglycan with diverse biological activities. Fatty acylation has been demonstrated to be essential for LAM to maintain its functional integri...

journal_title：Glycobiology

authors： Chatterjee D,Khoo KH

更新日期：1998-02-01 00:00:00