Abstract:
:Protein N- and O-glycosylation are well known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is known to influence antigen binding and effector functions, respectively. To study associations between antibody glycosylation profiles and (patho) physiological states as well as antibody functionality, advanced technologies and methods are required. In-depth structural characterization of antibody glycosylation usually relies on the separation and tandem mass spectrometric (MS) analysis of released glycans. Protein- and site-specific information, on the other hand, may be obtained by the MS analysis of glycopeptides. With the development of high-resolution mass spectrometers, antibody glycosylation analysis at the intact or middle-up level has gained more interest, providing an integrated view of different post-translational modifications (including glycosylation). Alongside the in-depth methods, there is also great interest in robust, high-throughput techniques for routine glycosylation profiling in biopharma and clinical laboratories. With an emphasis on IgG Fc glycosylation, several highly robust separation-based techniques are employed for this purpose. In this review, we describe recent advances in MS methods, separation techniques and orthogonal approaches for the characterization of immunoglobulin glycosylation in different settings. We put emphasis on the current status and expected developments of antibody glycosylation analysis in biomedical, biopharmaceutical and clinical research.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
de Haan N,Falck D,Wuhrer Mdoi
10.1093/glycob/cwz048subject
Has Abstractpub_date
2020-03-20 00:00:00pages
226-240issue
4eissn
0959-6658issn
1460-2423pii
5528791journal_volume
30pub_type
杂志文章,评审相关文献
GLYCOBIOLOGY文献大全abstract::Stage-specific embryonic antigen-1 (SSEA-1) is a well-known carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq054
更新日期:2010-08-01 00:00:00
abstract::N-acetylneuraminic acid (5-acetamido-3,5-dideoxy-d-glycero-d-galacto-non-2-ulosonic acid), which is the principal sialic acid family member of the non-2-ulosonic acids and their various derivatives, is often found at the terminal position on the glycan chains that adorn all vertebrate cells. This terminal position com...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz099
更新日期:2020-04-20 00:00:00
abstract::Proliferation of exponentially growing breast cancer cells (line Hs578T) was blocked specifically in G1 by 3-hydroxy-3-methylglutaryl Coenzyme A (HMG CoA) reductase inhibition, as well as by inhibition of N-linked glycosylation. As a consequence of these inhibitory conditions, the cells were synchronized in the G1 sta...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.5.475
更新日期:1993-10-01 00:00:00
abstract::A number of unusual and rare carbohydrates were tested as potential inhibitors of various glycosidases, as well as inhibitors of N-linked oligosaccharide processing. The best inhibitors of several arylglycosidases and of glucosidase I were L-xylulose and L-fructose. Both of these sugars showed some inhibitory activity...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/6.8.795
更新日期:1996-12-01 00:00:00
abstract::Lens culinaris lectin (LCA) is a useful probe for the detection in serum of a core-fucosylated alpha-fetoprotein, called AFP-L3 fraction, which is a well-known marker for the diagnosis and prognosis of hepatocellular carcinoma. Here we performed a systematic quantitative interaction analysis of LCA and its close homol...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp016
更新日期:2009-05-01 00:00:00
abstract::To study human alpha1,3/1,4fucosyltransferase (Fuc-TIII) as an alpha1,3 fucosyltransferase, we constructed two cell clones, C127-FT and C127-T-FT, by transfecting cDNA in parental (C127) or Polyoma T antigen expressing (C127-T) mouse cells, respectively. Both C127-FT and C127-T-FT clones express high levels of a fucos...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.1.83
更新日期:1999-01-01 00:00:00
abstract::The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm003
更新日期:2007-04-01 00:00:00
abstract::We have previously shown that varicella-zoster virus (VZV) and cytomegalovirus (CMV) infection of diploid human fibroblasts (HEL) results in neo-expression of Lewis antigens sialyl Lewis x (sLe(x)) and Lewis y (Le(y)), respectively, after transcriptional activation of different combinations of dormant human fucosyltra...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp057
更新日期:2009-08-01 00:00:00
abstract::Polysialic acid (PSA) is thought to attenuate neural cell adhesion molecule (NCAM) adhesion, thereby facilitating neural cell migration and regeneration. Although the expression of PSA has been shown to correlate with the progression of certain tumors such as small cell lung carcinoma, there have been no studies to de...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi071
更新日期:2005-09-01 00:00:00
abstract::Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf059
更新日期:2002-08-01 00:00:00
abstract::We have recently demonstrated that the mannose-binding lectins, namely banana lectin (BL) and garlic lectin (GL), interacted with the insulin receptors on M210B4 cells--an established mesenchymal cell line of murine marrow origin--and initiate mitogen-activated protein kinase kinase (MEK)-dependent extracellular signa...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq194
更新日期:2011-04-01 00:00:00
abstract::In this study, a sensitive, straightforward technique is developed for the analysis of glycoprotein O-linked oligosaccharides. Specifically, O-linked oligosaccharides of granulocyte colony-stimulating factor (G-CSF) are analysed by separating charged glycoforms using isoelectric focusing, electroblotting to polyvinyli...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.4.459
更新日期:1994-08-01 00:00:00
abstract::Prostate-specific antigen (PSA) is a glycoprotein secreted by prostate epithelial cells. PSA is currently used as a marker of prostate carcinoma because high levels of PSA are indicative of a tumor situation. However, PSA tests still suffer from a lack of specificity to distinguish between benign prostate hyperplasia ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg041
更新日期:2003-06-01 00:00:00
abstract::Gangliosides are able to bind to the epidermal growth factor receptor and inhibit its activation, but the mechanism of this inhibition is unknown. To address the role of receptor carbohydrates in facilitating interaction with gangliosides, we examined the ability of GM3 to bind the deglycosylated receptor and inhibit ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.7.515
更新日期:2001-07-01 00:00:00
abstract::In this work, focused on possible application of α-L-fucosidases from bacterial sources in the synthesis of α-L-fucosylated glycoconjugates, several nonpathogenic aerobic bacterial strains were screened for α-L-fucosidase activity. Among them Paenibacillus thiaminolyticus was confirmed as a potent producer of enzyme w...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt041
更新日期:2013-09-01 00:00:00
abstract::The product of p53-induced gene 1 is a member of the galectin family, i.e., galectin-7 (Gal-7). To move beyond structural data by X-ray diffraction, we initiated the study of the lectin by nuclear magnetic resonance (NMR) and circular dichroism spectroscopies, and molecular dynamics (MD) simulations. In concert, our r...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt005
更新日期:2013-05-01 00:00:00
abstract::The substrate specificity of the alpha-D-mannosidases of rat liver lysosome and cytosol was examined using oligosaccharides of the oligomannosidic type. The hydrolysis products were characterized by 400 MHz 1H-NMR spectroscopy. Both catabolic pathways occur in ordered ways, but are quite different. In fact, the lysoso...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/1.5.487
更新日期:1991-11-01 00:00:00
abstract::Sialyltransferases are a family of 20 gene products in mice and humans that transfer sialic acid from its activated precursor, CMP-sialic acid, to the terminus of glycoprotein and glycolipid acceptors. ST3Gal-II (coded by the St3gal2 gene) transfers sialic acid preferentially to the three positions of galactose on the...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww098
更新日期:2017-01-01 00:00:00
abstract::CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies), a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, Y0 myeloma and NS0 myeloma cell lines. These engineered cell lines were grown under different culture condit...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.8.813
更新日期:1995-12-01 00:00:00
abstract::Recombinant human erythropoietin (rHuEPO) is used abundantly in the clinic to stimulate red blood cell growth in anaemic patients. The efficacy of the drug depends strongly on the extent of sialylation of its carbohydrate moiety. Prompted by conflicting literature reports on the issue, we reinvestigated the structures...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.2.227
更新日期:1994-04-01 00:00:00
abstract::The substrate specificity of an alpha2,3-sialyltransferase (v-ST3Gal I) obtained from myxoma virus infected RK13 cells has been determined. Like mammalian sialyltransferase enzymes, the viral enzyme contains the characteristic L- and S-sialyl motif sequences in its catalytic domain. Analysis of the deduced amino acid ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.3.313
更新日期:2000-03-01 00:00:00
abstract::The rat liver sinusoidal endothelial cell (LEC) hyaluronan (HA) receptor was previously identified using a photoaffinity HA derivative (J. Biol. Chem., 267, 20451-20456, 1992). Two polypeptides with M(r) = 175,000 and 166,000, were consistently crosslinked, suggesting that the LEC HA receptor is an oligomer. Whether o...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.1.15
更新日期:1997-02-01 00:00:00
abstract::Viscumin of mistletoe (Viscum album L.) has a concentration-dependent activity profile unique to plant AB-toxins. It starts with lectin-dependent mitogenicity and then covers toxicity and cell agglutination, associated with shifts in the monomer/dimer equilibrium. Each lectin subunit harbors two sections for ligand co...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl017
更新日期:2006-10-01 00:00:00
abstract::The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj087
更新日期:2006-05-01 00:00:00
abstract::Trehalose synthase (TreS) catalyzes the reversible conversion of maltose into trehalose in mycobacteria as one of three biosynthetic pathways to this nonreducing disaccharide. Given the importance of trehalose to survival of mycobacteria, there has been considerable interest in understanding the enzymes involved in it...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt044
更新日期:2013-09-01 00:00:00
abstract::Pig to human xenotransplantation is considered a possible solution to the prevailing chronic lack of human donor organs for allotransplantation. The Galalpha1,3Gal determinant is the major porcine xenogeneic epitope causing hyperacute rejection following human antibody binding and complement activation. In order to ch...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.6.533
更新日期:1998-06-01 00:00:00
abstract::We investigated the localization of major gangliosides in adult rat brain by an immunofluorescence technique with mouse monoclonal antibodies (MAbs). Five MAbs (GMB16, GMR17, GGR12, GMR5 and GMR13) that specifically recognize gangliosides GM1, GD1a, GD1b, GT1b and GQ1b, respectively, were used. We have found that ther...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.2.137
更新日期:1993-04-01 00:00:00
abstract::Schistosomiasis caused by infection with parasitic helminths of Schistosoma spp. is a major global health problem due to inadequate treatment and lack of a vaccine. The immune response to schistosomes includes glycan antigens, which could be valuable diagnostic markers and vaccine targets. However, no precedent exists...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu027
更新日期:2014-07-01 00:00:00
abstract::In this paper, we present two complementary strategies for enrichment of glycoproteins on living cells that combine the desirable attributes of "robust enrichment" afforded by covalent-labeling techniques and "specificity for glycoproteins" typically provided by lectin or antibody affinity reagents. Our strategy invol...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws144
更新日期:2013-02-01 00:00:00
abstract::Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj092
更新日期:2006-06-01 00:00:00