A novel viral alpha2,3-sialyltransferase (v-ST3Gal I): transfer of sialic acid to fucosylated acceptors.

abstract：:To facilitate deciphering the information content in the glycome, thin film-coated photoactivatable surfaces were applied for covalent immobilization of glycans, glycoconjugates, or lectins in microarray formats. Light-induced immobilization of a series of bacterial exopolysaccharides on photoactivatable dextran-coate...

journal_title：Glycobiology

authors： Angeloni S,Ridet JL,Kusy N,Gao H,Crevoisier F,Guinchard S,Kochhar S,Sigrist H,Sprenger N

更新日期：2005-01-01 00:00:00

abstract：:We have elucidated the carbohydrate-binding profile of a non-monosaccharide-binding lectin named Eucheuma serra lectin (ESA)-2 from the red alga Eucheuma serra using a lectin-immobilized column and a centrifugal ultrafiltration-high performance liquid chromatography method with a variety of fluorescence-labeled oligos...

journal_title：Glycobiology

authors： Hori K,Sato Y,Ito K,Fujiwara Y,Iwamoto Y,Makino H,Kawakubo A

更新日期：2007-05-01 00:00:00

abstract：:The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...

journal_title：Glycobiology

authors： Kang NY,Kang SK,Lee YC,Choi HJ,Lee YS,Cho SY,Kim YS,Ko JH,Kim CH

更新日期：2006-05-01 00:00:00

abstract：:Proliferation of exponentially growing breast cancer cells (line Hs578T) was blocked specifically in G1 by 3-hydroxy-3-methylglutaryl Coenzyme A (HMG CoA) reductase inhibition, as well as by inhibition of N-linked glycosylation. As a consequence of these inhibitory conditions, the cells were synchronized in the G1 sta...

journal_title：Glycobiology

authors： Larsson O

更新日期：1993-10-01 00:00:00

abstract：:Galectin-1, the prototype of a family of beta-galactoside-binding proteins, has been implicated in a wide variety of biological processes. Data presented herein show that galectin-1 stimulates monocyte migration in a dose-dependent manner but is not chemotactic for macrophages. Galectin-1-induced monocyte chemotaxis i...

journal_title：Glycobiology

authors： Malik RK,Ghurye RR,Lawrence-Watt DJ,Stewart HJ

更新日期：2009-12-01 00:00:00

abstract：:Protein N- and O-glycosylation are well known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is known to influence antigen binding and effector functions, respectively. To study associations between antibody glycosylation profiles and (patho) physiological...

journal_title：Glycobiology

authors： de Haan N,Falck D,Wuhrer M

更新日期：2020-03-20 00:00:00

abstract：:Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed...

journal_title：Glycobiology

authors： van Wijk XM,Vallen MJ,van de Westerlo EM,Oosterhof A,Hao W,Versteeg EM,Raben J,Wismans RG,Smetsers TF,Dijkman HB,Schalkwijk J,van Kuppevelt TH

更新日期：2012-12-01 00:00:00

abstract：:We have characterized galectin family proteins in adult tissues of Xenopus laevis and purified 14-kDa and 36-kDa proteins from the liver. The liver galectins showed comparable hemagglutination activities to those of mammalian galectins. Furthermore, we isolated five galectin cDNAs from a Xenopus liver library. These c...

journal_title：Glycobiology

authors： Shoji H,Nishi N,Hirashima M,Nakamura T

更新日期：2002-03-01 00:00:00

abstract：:We recently described a novel fluorescent compound, 2-amino,6-amidobiotinyl-pyridine (BAP), that allows the tagging of oligosaccharides, their fractionation by reversed-phase HPLC with picomole scale detection, and the formation of functional neoglycoprotein equivalents with (strept) avidin for the detection of recept...

journal_title：Glycobiology

authors： Toomre DK,Varki A

更新日期：1994-10-01 00:00:00

abstract：:Bacterial protein glycosylation systems from varying species have been functionally reconstituted in Escherichia coli. Both N- and O-linked glycosylation pathways, in which the glycans are first assembled onto lipid carriers and subsequently transferred to acceptor proteins by an oligosaccharyltransferase (OTase), hav...

journal_title：Glycobiology

authors： Gebhart C,Ielmini MV,Reiz B,Price NL,Aas FE,Koomey M,Feldman MF

更新日期：2012-07-01 00:00:00

abstract：:Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated f...

journal_title：Glycobiology

authors： Simunovic J,Vilaj M,Trbojevic-Akmacic I,Momcilovic A,Vuckovic F,Gudelj I,Juric J,Nakic N,Lauc G,Pezer M

更新日期：2019-11-20 00:00:00

abstract：:Hyaluronan (HA) is a linear charged polysaccharide whose structure is made up of repeating disaccharide units. Apparently conflicting reports have been published about the nature of the helical structure of HA in the solid state. Recent developments in the field of molecular modeling of polysaccharides offer new oppor...

journal_title：Glycobiology

authors： Haxaire K,Braccini I,Milas M,Rinaudo M,Pérez S

更新日期：2000-06-01 00:00:00

abstract：:At weaning, the intestinal mucosa surface glycans change from predominantly sialylated to fucosylated. Intestinal adaptation from milk to solid food is regulated by intrinsic and extrinsic factors. The contribution by glucocorticoid, an intrinsic factor, and colonization by microbiota, an extrinsic factor, was measure...

journal_title：Glycobiology

authors： Nanthakumar NN,Meng D,Newburg DS

更新日期：2013-10-01 00:00:00

abstract：:Epithelial cells in the lining of the intestines play critical roles in maintaining homeostasis while challenged by dynamic and sudden changes in luminal contents. Given the high density of glycosylation that encompasses their extracellular surface, environmental changes may lead to extensive reorganization of membran...

journal_title：Glycobiology

authors： Park D,Xu G,Barboza M,Shah IM,Wong M,Raybould H,Mills DA,Lebrilla CB

更新日期：2017-09-01 00:00:00

abstract：:Sequestration of Plasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin 4-sulfate (C4S). A cytoadherence assay using chondroitin sulfate proteoglycans (CSPGs) is widely used for studying C4S-IRBC interactions. Bovine tracheal chondroitin sulfate A (CSA) preparation lack...

journal_title：Glycobiology

authors： Muthusamy A,Achur RN,Valiyaveettil M,Madhunapantula SV,Kakizaki I,Bhavanandan VP,Gowda CD

更新日期：2004-07-01 00:00:00

abstract：:Diabetes mellitus was induced in one group of rats by a single injection of streptozotocin. The glycemia, the body weight, and the blood systolic pressure were measured every week, and the 24 h urine volume and urinary excretions of creatinine, albumin and glycosaminoglycans were measured every 2 weeks. At the end of ...

journal_title：Glycobiology

authors： Cadaval RA,Kohlman O,Michelacci YM

更新日期：2000-02-01 00:00:00

abstract：:O-Acetylglucuronoxylans (AcGX) in Arabidopsis thaliana carry acetyl residues on the 2-O and/or 3-O positions of the xylopyranosyl (Xylp) units, but the distribution of different O-acetylated Xylp units is partly unclear. We studied a possible correlation of xylan acetylation and the activities of different glycosyltra...

journal_title：Glycobiology

authors： Chong SL,Virkki L,Maaheimo H,Juvonen M,Derba-Maceluch M,Koutaniemi S,Roach M,Sundberg B,Tuomainen P,Mellerowicz EJ,Tenkanen M

更新日期：2014-06-01 00:00:00

abstract：:Protozoan parasites of the genus Leishmania have a glycoconjugate surface coat (the glycocalyx) that acts as the interface between the parasite and its external environment. The principal components of the glycocalyx, the lipophosphoglycans and the glycoinositolphospholipids, have a variety of functions that facilitat...

journal_title：Glycobiology

authors： Smith DF,Rangarajan D

更新日期：1995-03-01 00:00:00

abstract：:The egg jellies of sea urchins contain sulfated polysaccharides with unusual structures, composed of linear chains of l-fucose or l-galactose with well-defined repetitive units. The specific pattern of sulfation and the position of the glycosidic bond vary among sulfated polysaccharides from different species. These p...

journal_title：Glycobiology

authors： Cinelli LP,Castro MO,Santos LL,Garcia CR,Vilela-Silva AC,Mourão PA

更新日期：2007-08-01 00:00:00

abstract：:To date, there is no complete structural characterization of human milk glycosaminoglycans (GAGs) available nor do any data exist on their composition in bovine milk. Total GAGs were determined on extracts from human and bovine milk. Samples were subjected to digestion with specific enzymes, treated with nitrous acid,...

journal_title：Glycobiology

authors： Coppa GV,Gabrielli O,Buzzega D,Zampini L,Galeazzi T,Maccari F,Bertino E,Volpi N

更新日期：2011-03-01 00:00:00

abstract：:The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...

journal_title：Glycobiology

authors： Hofinger ES,Spickenreither M,Oschmann J,Bernhardt G,Rudolph R,Buschauer A

更新日期：2007-04-01 00:00:00

abstract：:Sialic acid has long been considered to be the sole receptor for influenza virus. The viral hemagglutinin (HA) is known to bind cell surface sialic acid, and sialic acids on viral glyco-proteins are cleaved by the viral neuraminidase (NA) to promote efficient release of progeny virus particles. However, NWS-Mvi, a mut...

journal_title：Glycobiology

authors： Stray SJ,Cummings RD,Air GM

更新日期：2000-07-01 00:00:00

abstract：:Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...

journal_title：Glycobiology

authors： Hara-Kuge S,Seko A,Shimada O,Tosaka-Shimada H,Yamashita K

更新日期：2004-08-01 00:00:00

abstract：:In this work, we analyzed at high resolution the sugar-binding mode of the recombinant N-terminal ricin-B domain of the hemolytic protein LSLa (LSL(150)) from the mushroom Laetiporus sulphureus and also provide functional in vitro evidence suggesting that, together with its putative receptor-binding role, this module ...

journal_title：Glycobiology

authors： Angulo I,Acebrón I,de las Rivas B,Muñoz R,Rodríguez-Crespo I,Menéndez M,García P,Tateno H,Goldstein IJ,Pérez-Agote B,Mancheño JM

更新日期：2011-10-01 00:00:00

abstract：:Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...

journal_title：Glycobiology

authors： Rudloff S,Obermeier S,Borsch C,Pohlentz G,Hartmann R,Brösicke H,Lentze MJ,Kunz C

更新日期：2006-06-01 00:00:00

abstract：:β-Trefoil lectins are galactose/N-acetyl galactosamine specific lectins, which are widely distributed across all kingdoms of life and are known to perform several important functions. However, there is no report available on the characterization of these lectins from protozoans. We have performed structural and biophy...

journal_title：Glycobiology

authors： Khan F,Kurre D,Suguna K

更新日期：2020-07-20 00:00:00

abstract：:The synthesis of Galalpha1-3Gal-terminated oligosaccharides (alpha-Gal) epitopes has been interrupted during the course of evolution, starting with Old World primates. Partial sequences similar to the alpha1,3-galactosyltransferase (alpha1,3GalT) gene, which governs the synthesis of alpha-Gal epitopes, have been detec...

journal_title：Glycobiology

authors： Lantéri M,Giordanengo V,Vidal F,Gaudray P,Lefebvre JC

更新日期：2002-12-01 00:00:00

abstract：:Viscumin of mistletoe (Viscum album L.) has a concentration-dependent activity profile unique to plant AB-toxins. It starts with lectin-dependent mitogenicity and then covers toxicity and cell agglutination, associated with shifts in the monomer/dimer equilibrium. Each lectin subunit harbors two sections for ligand co...

journal_title：Glycobiology

authors： Jiménez M,André S,Siebert HC,Gabius HJ,Solís D

更新日期：2006-10-01 00:00:00

abstract：:In Campylobacter jejuni, the PglB oligosaccharyltransferase catalyzes the transfer of a heptasaccharide from a lipid donor to asparagine within the D/E-X1-N-X2-S/T sequon (X1,2 ≠ P) or releases this heptasaccharide as free oligosaccharides (fOS). Using available crystal structures and sequence alignments, we identifie...

journal_title：Glycobiology

authors： Barre Y,Nothaft H,Thomas C,Liu X,Li J,Ng KKS,Szymanski CM

更新日期：2017-10-01 00:00:00

abstract：:Rabies glycoprotein (RGP(WT)) contains N-glycosylation sequons at Asn(37), Asn(247), and Asn(319), although Asn(37) is not efficiently glycosylated. To examine N-glycan processing at Asn(247) and Asn(319), full-length glycosylation mutants, RGP(-2-) and RGP(--3), were expressed, and Endo H sensitivity was compared. Wh...

journal_title：Glycobiology

authors： Wojczyk BS,Takahashi N,Levy MT,Andrews DW,Abrams WR,Wunner WH,Spitalnik SL

更新日期：2005-06-01 00:00:00