Comprehensive N-glycosylation analysis of immunoglobulin G from dried blood spots.

Abstract:

:Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated from dried blood spots (DBSs), which was performed in a high-throughput mode. We compared the IgG N-glycan profiles originating from DBS with those originating from plasma, compared different media for DBS collection, evaluated analytical variation and assessed IgG N-glycan profile stability for different storage conditions. In conclusion, we show that DBSs are a good and stable source material for a robust IgG N-glycan analysis by ultra-performance liquid chromatography, suitable for blood sampling in conditions where no trained personnel and necessary laboratory equipment are available.

journal_name

Glycobiology

journal_title

Glycobiology

authors

Simunovic J,Vilaj M,Trbojevic-Akmacic I,Momcilovic A,Vuckovic F,Gudelj I,Juric J,Nakic N,Lauc G,Pezer M

doi

10.1093/glycob/cwz061

subject

Has Abstract

pub_date

2019-11-20 00:00:00

pages

817-821

issue

12

eissn

0959-6658

issn

1460-2423

pii

5542779

journal_volume

29

pub_type

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