Abstract:
:Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to secretory vesicles in the glandula parotis of the rat, where alpha-amylase also resided. A secretory vesicle fraction, prepared by Percoll density gradient centrifugation, contained both VIP36 and alpha-amylase. Moreover, alpha-amylase that was localized to these secretory vesicles contained high-mannose type glycans. In addition, VIP36 coprecipitated with alpha-amylase in an endo H treatment-sensitive manner. These results suggest that VIP36 is involved in the secretion of alpha-amylase in the rat parotid gland.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Hara-Kuge S,Seko A,Shimada O,Tosaka-Shimada H,Yamashita Kdoi
10.1093/glycob/cwh082subject
Has Abstractpub_date
2004-08-01 00:00:00pages
739-44issue
8eissn
0959-6658issn
1460-2423pii
cwh082journal_volume
14pub_type
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