Abstract:
:Cryptococcus neoformans, an opportunistic fungal pathogen, produces a glycan capsule to evade the immune system during infection. This definitive virulence factor is composed mainly of complex polysaccharides, which are made in the secretory pathway by reactions that utilize activated nucleotide sugar precursors. Although the pathways that synthesize these precursors are known, the identity and the regulation of the nucleotide sugar transporters (NSTs) responsible for importing them into luminal organelles remain elusive. The UDP-galactose transporter, Ugt1, was initially identified by homology to known UGTs and glycan composition analysis of ugt1Δ mutants. However, sequence is an unreliable predictor of NST substrate specificity, cells may express multiple NSTs with overlapping specificities, and NSTs may transport multiple substrates. Determining NST activity thus requires biochemical demonstration of function. We showed that Ugt1 transports both UDP-galactose and UDP-N-acetylgalactosamine in vitro. Deletion of UGT1 resulted in growth and mating defects along with altered capsule and cellular morphology. The mutant was also phagocytosed more readily by macrophages than wild-type cells and cleared more quickly in vivo and in vitro, suggesting a mechanism for the lack of virulence observed in mouse models of infection.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Li LX,Ashikov A,Liu H,Griffith CL,Bakker H,Doering TLdoi
10.1093/glycob/cww078subject
Has Abstractpub_date
2017-01-01 00:00:00pages
87-98issue
1eissn
0959-6658issn
1460-2423pii
cww078journal_volume
27pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the ER, disengages the nascent molecules fro...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi014
更新日期:2005-04-01 00:00:00
abstract::Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr151
更新日期:2012-05-01 00:00:00
abstract::Different classes of glycans are implicated as mediators of apical protein sorting in the secretory pathway of epithelial cells, but recent research indicates that sorting to the apical and basolateral surfaces may occur before completion of glycan synthesis. We have previously shown that a proteoglycan (PG) core prot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr069
更新日期:2011-11-01 00:00:00
abstract::In this study, a sensitive, straightforward technique is developed for the analysis of glycoprotein O-linked oligosaccharides. Specifically, O-linked oligosaccharides of granulocyte colony-stimulating factor (G-CSF) are analysed by separating charged glycoforms using isoelectric focusing, electroblotting to polyvinyli...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.4.459
更新日期:1994-08-01 00:00:00
abstract::The specificities of glycosaminoglycan (GAG) modification enzymes, particularly sulfotransferases, and the locations and concentrations of these enzymes in the Golgi apparatus give rise to the mature GAG polysaccharides that bind protein ligands. We studied the substrate specificities of sulfotransferases with a stabl...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt033
更新日期:2013-08-01 00:00:00
abstract::Porcine intestinal mucosal heparan sulfate was exhaustively depolymerized on a large scale using heparin lyase II (heparinase II) or heparin lyase III (heparitinase, EC 4.2.2.8). The oligosaccharide mixtures formed with each enzyme were fractionated by low pressure gel permeation chromatography. Size-uniform mixtures ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.2.231
更新日期:1997-03-01 00:00:00
abstract::In the present study, we studied anti-Alzheimer's disease (AD) activities of chondroitin sulfate (CS) oligosaccharides with different molecular weights. CS from shark cartilage was degraded by a recombinant CS endolyase, chondroitinase ABC I (CHSase ABC I), and CS disaccharide (DP2), tetrasaccharide (DP4), hexasacchar...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwaa096
更新日期:2020-10-09 00:00:00
abstract::Two different types of peptide:N-glycanase (PNGase) were identified in developing embryos of medaka fish ( Oryzias latipes ). Because the optimum pH values for their activities were acidic and neutral, they were designated as acid PNGase M and neutral PNGase M, respectively. The acid PNGase M corresponded to the enzym...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.9.887
更新日期:1999-09-01 00:00:00
abstract::Trypanosoma cruzi expresses a unique trans-sialidase that is responsible for the transfer of sialic acid from host glycoproteins and glycolipids to mucin-like glycoprotein acceptors on the parasite surface. The enzyme and the sialic acid acceptors are present in the mammalian forms of the parasite and in the parasite ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.6.625
更新日期:1995-09-01 00:00:00
abstract::Glycosphingolipids are expressed on the cell membrane and act as important factors in various events that occur across the plasma membrane. Lactosylceramide (LacCer) is synthesized from glucosylceramide and is a common precursor of various glycosphingolipids existing in whole body. Based on the enzyme purification, β1...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt054
更新日期:2013-10-01 00:00:00
abstract::Endo-alpha-D-mannosidase is a processing enzyme which in contrast to other glycosidases involved in the trimming of N-linked oligosaccharides of glycoproteins acts at an internal position by cleaving the linkage between the glucose-substituted mannose and the internal portion of the polymannose unit and thereby provid...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.4.579
更新日期:1997-06-01 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) is a heparan sulfate (HS)-binding factor. GDNF is produced by somatic Sertoli cells, where it signals to maintain spermatogonial stem cells (SSCs) and reproduction. Here, we investigate the roles of extracellular HS 6-O-endosulfatases (Sulfs), Sulf1 and Sulf2, in the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq133
更新日期:2011-02-01 00:00:00
abstract::Heparan sulfate is a polysaccharide belonging to the glycaminoglycan family. It interacts with numerous proteins of the extracellular matrix, in particular cellular growth factors. The number of experimental protein-heparin sulfate complexes obtained by crystallography or nuclear magnetic resonance is limited. Alterna...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr052
更新日期:2011-09-01 00:00:00
abstract::Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of th...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt091
更新日期:2014-02-01 00:00:00
abstract::Sialic acids cap the glycans of cell surface glycoproteins and glycolipids. They are involved in a multitude of biological processes and aberrant sialic acid expression is associated with several pathologies. Sialic acids modulate the characteristics and functions of glycoproteins and regulate cell-cell as well as cel...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz026
更新日期:2019-06-01 00:00:00
abstract::Muscle degenerative diseases such as Duchenne Muscular Dystrophy are incurable and treatment options are still restrained. Understanding the mechanisms and factors responsible for muscle degeneration and regeneration will facilitate the development of novel therapeutics. Several recent studies have demonstrated that G...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn079
更新日期:2008-11-01 00:00:00
abstract::We have characterized a glycosphingolipid (GSL) receptor for Staphylococcus enterotoxin-B (SEB) in cultured human kidney proximal tubular (PT) cells. Solid-phase binding of [125I]SEB to the GSL receptor was concentration dependent and was not displaceable by two structurally related toxins, such as staphylococcal ente...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.3.327
更新日期:1995-05-01 00:00:00
abstract::We have elucidated the carbohydrate-binding profile of a non-monosaccharide-binding lectin named Eucheuma serra lectin (ESA)-2 from the red alga Eucheuma serra using a lectin-immobilized column and a centrifugal ultrafiltration-high performance liquid chromatography method with a variety of fluorescence-labeled oligos...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm007
更新日期:2007-05-01 00:00:00
abstract::Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh082
更新日期:2004-08-01 00:00:00
abstract::The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn090
更新日期:2008-12-01 00:00:00
abstract::β-Trefoil lectins are galactose/N-acetyl galactosamine specific lectins, which are widely distributed across all kingdoms of life and are known to perform several important functions. However, there is no report available on the characterization of these lectins from protozoans. We have performed structural and biophy...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwaa001
更新日期:2020-07-20 00:00:00
abstract::Genetic polymorphism studies in humans provide unique opportunities to understand human biology and the mechanisms of diseases. Correlations between polymorphisms in the genes encoding human Siglecs and various diseases have been reported. Leading examples, such as the CD33 polymorphism associated with late-onset Alzh...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwu043
更新日期:2014-09-01 00:00:00
abstract::Shiga toxin (Stx) 2e of Stx-producing Escherichia coli (STEC) represents the major virulence factor responsible for the pig edema disease which is characterized by hemorrhagic lesions, neurological disorders and often fatal outcomes. Stx2e-producing strains from the intestine of slaughtered pigs (n = 3), feces of pigl...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws009
更新日期:2012-06-01 00:00:00
abstract::CMP-beta-N-acetylneuraminic acid (CMP-neuNAc) is the substrate for the sialylation of glycoconjugates by sialyltransferases in microbes and higher eukaryotes. CMP-neuNAc synthetase catalyzes the formation of this substrate, CMP-neuNAc, from CTP and neuNAc. In this report we describe the purification of CMP-neuNAc synt...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.5.481
更新日期:1999-05-01 00:00:00
abstract::Diabetes mellitus was induced in one group of rats by a single injection of streptozotocin. The glycemia, the body weight, and the blood systolic pressure were measured every week, and the 24 h urine volume and urinary excretions of creatinine, albumin and glycosaminoglycans were measured every 2 weeks. At the end of ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.2.185
更新日期:2000-02-01 00:00:00
abstract::Shigella flexneri is a gram-negative bacterium responsible for serious enteric infections that occur mainly in the terminal ileum and colon. High interest in Shigella, as a human pathogen, is driven by its antibiotic resistance and the necessity to develop a vaccine against its infections. Vaccines of the last generat...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm140
更新日期:2008-03-01 00:00:00
abstract::Phytohaemagglutinin (PHA) is the major lectin present in the seeds of the common bean, Phaseolus vulgaris, and PHA-L is the leucocyte-agglutinating form of this lectin. This tetrameric glycoprotein accumulates in the vacuoles of storage parenchyma cells. Based on amino acid sequence comparisons of legume lectins and t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.6.581
更新日期:1993-12-01 00:00:00
abstract::Based on chemical analysis, we have previously concluded that the biologically important lipoarabinomannan (LAM) and lipomannan (LM) from Mycobacterium are multiglycosylated forms of the phosphatidylinositol mannosides (PIMs), the characteristic cell envelope mannophosphoinositides of mycobacteria. Using definitive an...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.1.117
更新日期:1995-02-01 00:00:00
abstract::Glycans mediate a wide variety of biological roles via recognition by glycan-binding proteins (GBPs). Comprehensive knowledge of such interaction is thus fundamental to glycobiology. While the primary binding feature of GBPs can be easily uncovered by using a simple glycan microarray harboring limited numbers of glyca...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz100
更新日期:2020-04-20 00:00:00
abstract::Xylosylated and core alpha1,3-fucosylated N-glycans from plants are immunogenic, and they play a still obscure role in allergy and in the field of plant-made protein pharmaceuticals. We immunized mice to generate monoclonal antibodies (mAbs) binding plant N-glycans specifically via the epitope containing either the xy...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj071
更新日期:2006-04-01 00:00:00