Carbohydrate-binding properties of human neo-CRP and its relationship to phosphorylcholine-binding site.

abstract：:The trehalose-P synthase was purified to near homogeneity from the cytoplasmic fraction of Mycobacterium smegmatis. At the final stage of purification, the enzyme preparation showed one major band of 59 kDa on SDS gels. The 59 kDa band became labeled with N3-UDP[32P]-glucose, and this labeling was inhibited in a conce...

journal_title：Glycobiology

authors： Pan YT,Drake RR,Elbein AD

更新日期：1996-06-01 00:00:00

abstract：:Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structura...

journal_title：Glycobiology

authors： Kudoh A,Okawa Y,Shibata N

更新日期：2015-01-01 00:00:00

abstract：:Cell-surface glycans are functional docking sites for tissue lectins such as the members of the galectin family. This interaction triggers a wide variety of responses; hence, there is a keen interest in defining its structural features. Toward this aim, we have used enzyme-linked lectinosorbent (ELLSA) and inhibition ...

journal_title：Glycobiology

authors： Wu AM,Singh T,Wu JH,Lensch M,André S,Gabius HJ

更新日期：2006-06-01 00:00:00

abstract：:The sialic acid-specific cytotoxic lectin viscumin and its recombinant equivalent rViscumin specifically bind to CD75s-gangliosides with terminal Neu5Acα6Galβ4GlcNAc sequence. We, therefore, comparatively analyzed the content of CD75s-gangliosides and closely related iso-CD75s-gangliosides (terminated by Neu5Acα3Galβ4...

journal_title：Glycobiology

authors： Souady J,Hülsewig M,Distler U,Haier J,Denz A,Pilarsky C,Senninger N,Dreisewerd K,Peter-Katalinic J,Müthing J

更新日期：2011-05-01 00:00:00

abstract：:Isolation of glycopeptides utilizing hydrogen bonding between glycopeptide glycans and a carbohydrate-gel matrix in the organic phase is useful for site-specific characterization of oligosaccharides of glycoproteins, when combined with mass spectrometry. In this study, recovery of glycopeptides was improved by includi...

journal_title：Glycobiology

authors： Tajiri M,Yoshida S,Wada Y

更新日期：2005-12-01 00:00:00

abstract：:An ultrasensitive method for analysis of polysialic acid (polySia) chains, using fluorescence-assisted high-performance liquid chromatography was developed. The new method is a substantial improvement of our earlier method in which the reducing terminal Sia residues of a homologous series of oligo/polySia hydrolytical...

journal_title：Glycobiology

authors： Inoue S,Lin SL,Lee YC,Inoue Y

更新日期：2001-09-01 00:00:00

abstract：:A protocol is described for uniform 13C labelling of terminal galactose residues of the glycan chains of glycoproteins, using an enzymatic method which does not perturb the protein. The technique is illustrated by application to the biantennary N-linked glycan chains attached at Asn 297 of immunoglobulin G (IgG). Isot...

journal_title：Glycobiology

authors： Gilhespy-Muskett AM,Partridge J,Jefferis R,Homans SW

更新日期：1994-08-01 00:00:00

abstract：:Invariant natural killer T (iNKT) cells recognize glycolipid antigens bound to CD1d molecules on antigen-presenting cells. Therapeutic activation of iNKT cells with the xenogeneic glycolipid α-galactosylceramide (α-GalCer) can prevent and reverse tumor growth in murine models, but clinical trials using α-GalCer-stimul...

journal_title：Glycobiology

authors： Melo AM,Zhang L,Dockry ÉF,Petrasca A,Ghnewa YG,Breen EP,Morrissey ME,O'Reilly C,Bruen R,O'Meara A,Lysaght J,Zhu X,Doherty DG

更新日期：2018-07-01 00:00:00

abstract：:The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transf...

journal_title：Glycobiology

authors： Chan NW,Stangier K,Sherburne R,Taylor DE,Zhang Y,Dovichi NJ,Palcic MM

更新日期：1995-10-01 00:00:00

abstract：:The common O-glycan core structure in animal glycoproteins is the core 1 disaccharide Galbeta1-3GalNAcalpha1-Ser/Thr, which is generated by the addition of Gal to GalNAcalpha1-Ser/Thr by core 1 UDP-alpha-galactose (UDP-Gal):GalNAcalpha1-Ser/Thr beta1,3-galactosyltransferase (core 1 beta3-Gal-T or T-synthase, EC2.4.1.1...

journal_title：Glycobiology

authors： Ju T,Zheng Q,Cummings RD

更新日期：2006-10-01 00:00:00

abstract：:The human intestine houses a dense microbial ecosystem in which the struggle for nutrients creates a continual and dynamic selective force. Host-produced mucus glycans provide a ubiquitous source of carbon and energy for microbial species. Not surprisingly, many gut resident bacteria have become highly adapted to effi...

journal_title：Glycobiology

authors： Marcobal A,Southwick AM,Earle KA,Sonnenburg JL

更新日期：2013-09-01 00:00:00

abstract：:To study human alpha1,3/1,4fucosyltransferase (Fuc-TIII) as an alpha1,3 fucosyltransferase, we constructed two cell clones, C127-FT and C127-T-FT, by transfecting cDNA in parental (C127) or Polyoma T antigen expressing (C127-T) mouse cells, respectively. Both C127-FT and C127-T-FT clones express high levels of a fucos...

journal_title：Glycobiology

authors： Valli M,Bardoni A,Trinchera M

更新日期：1999-01-01 00:00:00

abstract：:Glycation of immunoglobulin G (IgG) can result from incubation with a reducing sugar in vitro or during circulation in vivo. Upon injection of a recombinantly produced human therapeutic IgG into humans, changes in the glycation levels could be observed as a function of circulation time. Mass changes on the individual ...

journal_title：Glycobiology

authors： Goetze AM,Liu YD,Arroll T,Chu L,Flynn GC

更新日期：2012-02-01 00:00:00

abstract：:Algal fucoidan is an alpha-L-fucose-based polysaccharide endowed with important biological properties for which the structure has not yet been fully elucidated. In an attempt to implement new enzymatic tools for structural study of this polysaccharide, we have found a fucosidase activity in the digestive glands of the...

journal_title：Glycobiology

authors： Berteau O,McCort I,Goasdoué N,Tissot B,Daniel R

更新日期：2002-04-01 00:00:00

abstract：:A large family of UDP-GalNAc:polypeptide GalNAc transferases (ppGalNAc-Ts) initiates and defines sites of mucin-type Ser/Thr-O-GalNAc glycosylation. Family members have been classified into peptide- and glycopeptide-preferring subfamilies, although both families possess variable activities against glycopeptide substra...

journal_title：Glycobiology

authors： Revoredo L,Wang S,Bennett EP,Clausen H,Moremen KW,Jarvis DL,Ten Hagen KG,Tabak LA,Gerken TA

更新日期：2016-04-01 00:00:00

abstract：:A glycosyltransferase was fused to the yeast cell wall protein Pir, which forms the Pir1-4 protein family and is incorporated into the cell wall by an unknown linkage to be displayed at the yeast cell surface. We first expressed the PIR1-HA-gma12+ fusion, in which gma12+ encodes alpha-1,2-galactosyltransferase from th...

journal_title：Glycobiology

authors： Abe H,Shimma Y,Jigami Y

更新日期：2003-02-01 00:00:00

abstract：:Trypanosoma cruzi expresses a unique trans-sialidase that is responsible for the transfer of sialic acid from host glycoproteins and glycolipids to mucin-like glycoprotein acceptors on the parasite surface. The enzyme and the sialic acid acceptors are present in the mammalian forms of the parasite and in the parasite ...

journal_title：Glycobiology

authors： Amino R,Serrano AA,Morita OM,Pereira-Chioccola VL,Schenkman S

更新日期：1995-09-01 00:00:00

abstract：:Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. Today, only one recombinant human Epo (rHuEpo), produced in CHO cell line, is extensively used in t...

journal_title：Glycobiology

authors： Cointe D,Béliard R,Jorieux S,Leroy Y,Glacet A,Verbert A,Bourel D,Chirat F

更新日期：2000-05-01 00:00:00

abstract：:Viscumin of mistletoe (Viscum album L.) has a concentration-dependent activity profile unique to plant AB-toxins. It starts with lectin-dependent mitogenicity and then covers toxicity and cell agglutination, associated with shifts in the monomer/dimer equilibrium. Each lectin subunit harbors two sections for ligand co...

journal_title：Glycobiology

authors： Jiménez M,André S,Siebert HC,Gabius HJ,Solís D

更新日期：2006-10-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the alg2 mutation causes temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol (Jackson et al., Arch. Biochem. Biophys., 272, 203-209, 1989; Huffaker and Robbins, Proc. Natl. Acad. Sci. USA, 80, 7466-7470, 1983). A gene havi...

journal_title：Glycobiology

authors： Jackson BJ,Kukuruzinska MA,Robbins P

更新日期：1993-08-01 00:00:00

abstract：:Shigella flexneri is the major human pathogen causing shigellosis. O-antigens of all S. flexneri serotypes (except for serotype 6) share the →2)-α-l-Rhap(III)-(1 → 2)-α-l-Rhap(II)-(1 → 3)-α-l-Rhap(I)-(1 → 3)-β-d-GlcpNAc-(1→ basic O-unit, whereas differences between the serotypes are conferred by phage-encoded glucosyl...

journal_title：Glycobiology

authors： Knirel YA,Lan R,Senchenkova SN,Wang J,Shashkov AS,Wang Y,Perepelov AV,Xiong Y,Xu J,Sun Q

更新日期：2013-04-01 00:00:00

abstract：:Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and de...

journal_title：Glycobiology

authors： Wu ZL,Person AD,Burton AJ,Singh R,Burroughs B,Fryxell D,Tatge TJ,Manning T,Wu G,Swift KAD,Kalabokis V

更新日期：2019-10-21 00:00:00

abstract：:The methylotrophic yeast, Pichia pastoris, is an important organism used for the production of therapeutic proteins. However, the presence of fungal-like glycans, such as those containing β-mannose (Man) linkages, can elicit an immune response or bind to Man receptors, thus reducing their efficacy. Recent studies have...

journal_title：Glycobiology

authors： Hopkins D,Gomathinayagam S,Rittenhour AM,Du M,Hoyt E,Karaveg K,Mitchell T,Nett JH,Sharkey NJ,Stadheim TA,Li H,Hamilton SR

更新日期：2011-12-01 00:00:00

abstract：:Angiogenesis is associated with growth, invasion, and metastasis of human solid tumors. Aberrant activation of endothelial cells and induction of microvascular permeability by a vascular endothelial growth factor (VEGF) receptor-2 (VEGFR-2) signaling pathway is observed in pathological angiogenesis including tumor, wo...

journal_title：Glycobiology

authors： Chung TW,Kim SJ,Choi HJ,Kim KJ,Kim MJ,Kim SH,Lee HJ,Ko JH,Lee YC,Suzuki A,Kim CH

更新日期：2009-03-01 00:00:00

abstract：:Neisseria meningitidis (Nm) is a leading cause of bacterial meningitis and sepsis. A key feature in pathogenicity is the capsular polysaccharide (CPS) that prevents complement activation and thus supports bacterial survival in the host. Twelve serogroups characterized by immunologically and structurally different CPSs...

journal_title：Glycobiology

authors： Fiebig T,Berti F,Freiberger F,Pinto V,Claus H,Romano MR,Proietti D,Brogioni B,Stummeyer K,Berger M,Vogel U,Costantino P,Gerardy-Schahn R

更新日期：2014-02-01 00:00:00

abstract：:The glycobiology of the cestodes, a class of parasitic flatworms, is still largely unexplored. An important cestode species is Echinococcus granulosus, the tissue-dwelling larval stage of which causes hydatid disease. The E. granulosus larva is protected from the host by a massive mucin-based extracellular matrix term...

journal_title：Glycobiology

authors： Lin G,Todeschini AR,Koizumi A,Neves JL,González H,Dematteis S,Hada N,Previato JO,Ferreira F,Mendonça-Previato L,Díaz A

更新日期：2013-04-01 00:00:00

abstract：:Sialic-acid-binding immunoglobulin-like lectins (Siglecs) are a family of transmembrane receptors that are well documented to play roles in regulation of innate and adaptive immune responses. To see whether the features that define the molecular recognition of sialic acid were found in other sialic-acid-binding protei...

journal_title：Glycobiology

authors： Rademacher C,Bru T,McBride R,Robison E,Nycholat CM,Kremer EJ,Paulson JC

更新日期：2012-08-01 00:00:00

abstract：:We recently described a novel fluorescent compound, 2-amino,6-amidobiotinyl-pyridine (BAP), that allows the tagging of oligosaccharides, their fractionation by reversed-phase HPLC with picomole scale detection, and the formation of functional neoglycoprotein equivalents with (strept) avidin for the detection of recept...

journal_title：Glycobiology

authors： Toomre DK,Varki A

更新日期：1994-10-01 00:00:00

abstract：:Escherichia coli K92 produces a capsular polysialic acid with alternating alpha2,8 alpha2,9 NeuNAc linkages. This polysaccharide is cross-reactive with the neuroinvasive pathogen Neisseria meningitidis Group C. The K92 polysialyltransferase (PST) catalyzes the synthesis of the polysialic acid with alternating linkages...

journal_title：Glycobiology

authors： Vionnet J,Vann WF

更新日期：2007-07-01 00:00:00

abstract：:Mouse macrophage galactose/N-acetylgalactosamine-specific C-type lectin (MMGL) is a type II transmembrane glycoprotein belonging to the C-type lectin family. Our development of monoclonal antibodies led us to discover that a calcium-dependent conformational change is detected by an antibody (termed mAb LOM-11) and tha...

journal_title：Glycobiology

authors： Hosoi T,Imai Y,Irimura T

更新日期：1998-08-01 00:00:00