A novel 13C isotopic labelling strategy for probing the structure and dynamics of glycan chains in situ on glycoproteins.

abstract：:In this paper, we present two complementary strategies for enrichment of glycoproteins on living cells that combine the desirable attributes of "robust enrichment" afforded by covalent-labeling techniques and "specificity for glycoproteins" typically provided by lectin or antibody affinity reagents. Our strategy invol...

journal_title：Glycobiology

authors： Ramya TN,Weerapana E,Cravatt BF,Paulson JC

更新日期：2013-02-01 00:00:00

abstract：:The oligosaccharide chains (glycans) attached to cell surface and extracellular proteins and lipids are known to mediate many important biological roles. However, for many glycans, there are still no evident functions that are of obvious benefit to the organism that synthesizes them. There is also no clear explanation...

journal_title：Glycobiology

authors： Gagneux P,Varki A

更新日期：1999-08-01 00:00:00

abstract：:Glycolipids are important components of almost all biological membranes. They possess unique properties that have only been incompletely characterized so far. The plant glycolipid digalactosyldiacylglycerol (DGDG) strongly influences the physical behavior of phospholipid model membranes in both the dry and hydrated st...

journal_title：Glycobiology

authors： Popova AV,Hincha DK

更新日期：2005-11-01 00:00:00

abstract：:Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion. The activity was solubilized by t...

journal_title：Glycobiology

authors： Ziegler FD,Trimble RB

更新日期：1991-12-01 00:00:00

abstract：:Apolipoprotein C-III (apoCIII) is a small glycoprotein with a single mucin-type core-1 oligosaccharide and is analyzed by isoelectric focusing (IEF) for the diagnosis of genetic defects in O-glycan biosynthesis such as congenital disorders of glycosylation. In the present study, mass spectrometry of apoCIII, after a s...

journal_title：Glycobiology

authors： Wada Y,Kadoya M,Okamoto N

更新日期：2012-08-01 00:00:00

abstract：:The content of sulfated glycans having 6-O-sulfated GlcNAc residues alters in the course of colonic carcinogenesis. We previously characterized two GlcNAc 6-O-sulfotransferases (SulTs), SulT-a and -b, expressed in colonic normal tissues and adenocarcinomas [Seko et al. (2000) Glycobiology, 10, 919-929]. Levels of the ...

journal_title：Glycobiology

authors： Seko A,Nagata K,Yonezawa S,Yamashita K

更新日期：2002-06-01 00:00:00

abstract：:Sialic-acid-binding immunoglobulin-like lectins (Siglecs) are a family of transmembrane receptors that are well documented to play roles in regulation of innate and adaptive immune responses. To see whether the features that define the molecular recognition of sialic acid were found in other sialic-acid-binding protei...

journal_title：Glycobiology

authors： Rademacher C,Bru T,McBride R,Robison E,Nycholat CM,Kremer EJ,Paulson JC

更新日期：2012-08-01 00:00:00

abstract：:Poly-N-acetyllactosamine extensions on N- and O-linked glycans are increasingly recognized as biologically important structural features, but access to these structures has not been widely available. Here, we report a detailed substrate specificity and catalytic efficiency of the bacterial β3-N-acetylglucosaminyltrans...

journal_title：Glycobiology

authors： Peng W,Pranskevich J,Nycholat C,Gilbert M,Wakarchuk W,Paulson JC,Razi N

更新日期：2012-11-01 00:00:00

abstract：:Changes in mucin protein expression and in glycosylation are common features in pre-neoplastic lesions and cancer and are therefore used as cancer-associated markers. De novo expression of intestinal mucin MUC2 and cancer-associated sialyl-Tn antigen are frequently observed in intestinal metaplasia (IM) and gastric ca...

journal_title：Glycobiology

authors： Conze T,Carvalho AS,Landegren U,Almeida R,Reis CA,David L,Söderberg O

更新日期：2010-02-01 00:00:00

abstract：:Sequestration of Plasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin 4-sulfate (C4S). A cytoadherence assay using chondroitin sulfate proteoglycans (CSPGs) is widely used for studying C4S-IRBC interactions. Bovine tracheal chondroitin sulfate A (CSA) preparation lack...

journal_title：Glycobiology

authors： Muthusamy A,Achur RN,Valiyaveettil M,Madhunapantula SV,Kakizaki I,Bhavanandan VP,Gowda CD

更新日期：2004-07-01 00:00:00

abstract：:Human skin fibroblasts were incubated in the presence of 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU). The culture medium was recovered and Xyl-MU derivatives which were initiated by the Xyl-MU acting as a primer were purified. As a result, a novel Xyl-MU derivative was isolated, in addition to previously reported Xy...

journal_title：Glycobiology

authors： Tazawa T,Takagaki K,Matsuya H,Nakamura T,Sasaki M,Endo M

更新日期：1998-09-01 00:00:00

abstract：:The core oligosaccharide Glc(3)Man(9)GlcNAc(2) is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (ER). The first step of this assembly pathway on the ER luminal side is mediated by ALG3 (asparagine-linked glycosylation ...

journal_title：Glycobiology

authors： Kajiura H,Seki T,Fujiyama K

更新日期：2010-06-01 00:00:00

abstract：:The structures of the pectic polysaccharide rhamnogalacturonan II (RG-II) pectin constituent are remarkably evolutionary conserved in all plant species. At least 12 different glycosyl residues are present in RG-II. Among them is the seldom eight-carbon sugar 3-deoxy-d-manno-octulosonic acid (Kdo) whose biosynthetic pa...

journal_title：Glycobiology

authors： Séveno M,Séveno-Carpentier E,Voxeur A,Menu-Bouaouiche L,Rihouey C,Delmas F,Chevalier C,Driouich A,Lerouge P

更新日期：2010-05-01 00:00:00

abstract：:Prostate-specific antigen (PSA) is a glycoprotein secreted by prostate epithelial cells. PSA is currently used as a marker of prostate carcinoma because high levels of PSA are indicative of a tumor situation. However, PSA tests still suffer from a lack of specificity to distinguish between benign prostate hyperplasia ...

journal_title：Glycobiology

authors： Peracaula R,Tabarés G,Royle L,Harvey DJ,Dwek RA,Rudd PM,de Llorens R

更新日期：2003-06-01 00:00:00

abstract：:N-acetylglucosaminyltransferase V, encoded by the Mgat5 gene, plays an important regulatory role in the synthesis of complex N-glycans, including the Lewis antigens. The elevated expression of this enzyme is regulated during development and is highly associated with cellular transformation and malignancy. In addition,...

journal_title：Glycobiology

authors： Alverez K,Haswell C,St Clair M,Perng GS,Shorebah M,Pierce M,Fregien N

更新日期：2002-07-01 00:00:00

abstract：:In Campylobacter jejuni, the PglB oligosaccharyltransferase catalyzes the transfer of a heptasaccharide from a lipid donor to asparagine within the D/E-X1-N-X2-S/T sequon (X1,2 ≠ P) or releases this heptasaccharide as free oligosaccharides (fOS). Using available crystal structures and sequence alignments, we identifie...

journal_title：Glycobiology

authors： Barre Y,Nothaft H,Thomas C,Liu X,Li J,Ng KKS,Szymanski CM

更新日期：2017-10-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the alg2 mutation causes temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol (Jackson et al., Arch. Biochem. Biophys., 272, 203-209, 1989; Huffaker and Robbins, Proc. Natl. Acad. Sci. USA, 80, 7466-7470, 1983). A gene havi...

journal_title：Glycobiology

authors： Jackson BJ,Kukuruzinska MA,Robbins P

更新日期：1993-08-01 00:00:00

abstract：:Sialic acids can be modified by O-acetyl esters at the 7- and/or 9-position, altering recognition by antibodies, lectins and viruses. 9(7)-O-acetylation is mediated by a sialic acid-specific O-acetyltransferase, which has proven difficult to purify. Two groups have recently isolated cDNAs possibly encoding this enzyme...

journal_title：Glycobiology

authors： Shi WX,Chammas R,Varki A

更新日期：1998-02-01 00:00:00

abstract：:Spectrofluorimetry experiments using synthetic trisaccharides indicate that in compounds that display affinity for antithrombin III (AT-III), a unique trisaccharide sequence plays the key role in the early recognition, and the first step of AT-III activation. Added to previous observations, these new results suggest t...

journal_title：Glycobiology

authors： Petitou M,Barzu T,Herault JP,Herbert JM

更新日期：1997-04-01 00:00:00

abstract：:Affinity-purified glycopeptides were prepared from Trypanosoma cruzi using the carbohydrate-specific monoclonal antibody WIC29.26. These glycopeptides contain rhamnose, fucose, xylose, and galactose, in the ratio 1:1:2:3. A series of oligosaccharides was released from the glycopeptides by mild acid hydrolysis, while, ...

journal_title：Glycobiology

authors： Haynes PA,Ferguson MA,Cross GA

更新日期：1996-12-01 00:00:00

abstract：:There is increasing evidence that secretory fluids such as tears, saliva and milk play an important role in protecting the human body from infection via a washing mechanism involving glycan-mediated adhesion of potential pathogens to secretory glycoproteins. Interaction of sweat with bacteria is well established as th...

journal_title：Glycobiology

authors： Peterson RA,Gueniche A,Adam de Beaumais S,Breton L,Dalko-Csiba M,Packer NH

更新日期：2016-03-01 00:00:00

abstract：:The glycobiology of Schistosoma mansoni is dominated by developmentally regulated expression of various fucosylated structures, most notably the Lewis X epitope and a multifucosylated sequence, Fuc alpha1-->2Fuc alpha1-->, in its various forms. For the infective cercarial stage, Lewis X has been structurally identifie...

journal_title：Glycobiology

authors： Huang HH,Tsai PL,Khoo KH

更新日期：2001-05-01 00:00:00

abstract：:Endo-alpha-D-mannosidase is a processing enzyme which in contrast to other glycosidases involved in the trimming of N-linked oligosaccharides of glycoproteins acts at an internal position by cleaving the linkage between the glucose-substituted mannose and the internal portion of the polymannose unit and thereby provid...

journal_title：Glycobiology

authors： Dairaku K,Spiro RG

更新日期：1997-06-01 00:00:00

abstract：:Heparan sulfate (HS) is a sulfated polysaccharide that plays a key role in morphogenesis, physiology and pathogenesis. The biosynthesis of HS takes place in the Golgi apparatus by a group of enzymes that polymerize, epimerize and sulfate the sugar chain. This biosynthetic process introduces varying degrees of sulfate ...

journal_title：Glycobiology

authors： Gesteira TF,Coulson-Thomas VJ

更新日期：2018-11-01 00:00:00

abstract：:Glycoside hydrolases are particularly abundant in all areas of metabolism as they are involved in the degradation of natural polysaccharides and glycoconjugates. These enzymes are classified into 133 families (CAZy server, http://www.cazy.org) in which members of each family have a similar structure and catalytic mech...

journal_title：Glycobiology

authors： André-Miral C,Koné FM,Solleux C,Grandjean C,Dion M,Tran V,Tellier C

更新日期：2015-04-01 00:00:00

abstract：:The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...

journal_title：Glycobiology

authors： De Castro C,Carannante A,Lanzetta R,Liparoti V,Molinaro A,Parrilli M

更新日期：2006-12-01 00:00:00

abstract：:beta1-6 GlcNAc branching, a product of N-acetylglucosaminyltransferase V (GnT-V), is a key structure that is associated with malignant transformations and cancer metastasis. Although a number of reports concerning tumor metastasis-related glycoproteins that contain beta1-6 GlcNAc branching have appeared, the precise f...

journal_title：Glycobiology

authors： Ihara S,Miyoshi E,Nakahara S,Sakiyama H,Ihara H,Akinaga A,Honke K,Dickson RB,Lin CY,Taniguchi N

更新日期：2004-02-01 00:00:00

abstract：:It is demonstrated with glycopeptides of the polymorphic epithelial mucin (MUC1) that post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) is a fast, highly sensitive, and reproducible method for the localization of O-glycosylation sites by reflectron time-of-flight (TOF) mass spectrometry. We hav...

journal_title：Glycobiology

authors： Goletz S,Thiede B,Hanisch FG,Schultz M,Peter-Katalinic J,Müller S,Seitz O,Karsten U

更新日期：1997-10-01 00:00:00

abstract：:Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed...

journal_title：Glycobiology

authors： van Wijk XM,Vallen MJ,van de Westerlo EM,Oosterhof A,Hao W,Versteeg EM,Raben J,Wismans RG,Smetsers TF,Dijkman HB,Schalkwijk J,van Kuppevelt TH

更新日期：2012-12-01 00:00:00

abstract：:Intelectin is an extracellular animal lectin found in chordata. Although human and mouse intelectin-1 recognize galactofuranosyl residues included in cell walls of various microorganisms, the physiological function of mammalian intelectin had been unclear. In this study, we found that human intelectin-1 was a serum pr...

journal_title：Glycobiology

authors： Tsuji S,Yamashita M,Hoffman DR,Nishiyama A,Shinohara T,Ohtsu T,Shibata Y

更新日期：2009-05-01 00:00:00