A Siglec-like sialic-acid-binding motif revealed in an adenovirus capsid protein.

abstract：:Glycosyltransferases, usually residing within the intracellular secretory apparatus, also circulate in the blood. Many of these blood-borne glycosyltransferases are associated with pathological states, including malignancies and inflammatory conditions. Despite the potential for dynamic modifications of glycans on dis...

journal_title：Glycobiology

authors： Lee-Sundlov MM,Ashline DJ,Hanneman AJ,Grozovsky R,Reinhold VN,Hoffmeister KM,Lau JT

更新日期：2017-01-01 00:00:00

abstract：:Algal fucoidan is an alpha-L-fucose-based polysaccharide endowed with important biological properties for which the structure has not yet been fully elucidated. In an attempt to implement new enzymatic tools for structural study of this polysaccharide, we have found a fucosidase activity in the digestive glands of the...

journal_title：Glycobiology

authors： Berteau O,McCort I,Goasdoué N,Tissot B,Daniel R

更新日期：2002-04-01 00:00:00

abstract：:L-Selectin is a lectin-like receptor on lymphocytes which mediates their attachment to high endothelial venules (HEV) within lymph nodes. Previous work has identified HEV-associated endothelial ligands for L-selectin as sialylated, fucosylated and sulphated glycoproteins of approximately 50 kDa and approximately 90 kD...

journal_title：Glycobiology

authors： Imai Y,Lasky LA,Rosen SD

更新日期：1992-08-01 00:00:00

abstract：:Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. Today, only one recombinant human Epo (rHuEpo), produced in CHO cell line, is extensively used in t...

journal_title：Glycobiology

authors： Cointe D,Béliard R,Jorieux S,Leroy Y,Glacet A,Verbert A,Bourel D,Chirat F

更新日期：2000-05-01 00:00:00

abstract：:We report the production of biologically active hyaluronan (HA) oligosaccharides labeled with the fluorophore 2-aminobenzoic acid (2AA). Oligosaccharides from 4 to 40 residues in length were purified to homogeneity by ion exchange chromatography using a logarithmic gradient. Molecular weight and purity characterizatio...

journal_title：Glycobiology

authors： Seyfried NT,Blundell CD,Day AJ,Almond A

更新日期：2005-03-01 00:00:00

abstract：:A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes...

journal_title：Glycobiology

authors： Nimrichter L,Gargir A,Gortler M,Altstock RT,Shtevi A,Weisshaus O,Fire E,Dotan N,Schnaar RL

更新日期：2004-02-01 00:00:00

abstract：:Gangliosides are able to bind to the epidermal growth factor receptor and inhibit its activation, but the mechanism of this inhibition is unknown. To address the role of receptor carbohydrates in facilitating interaction with gangliosides, we examined the ability of GM3 to bind the deglycosylated receptor and inhibit ...

journal_title：Glycobiology

authors： Wang XQ,Sun P,O'Gorman M,Tai T,Paller AS

更新日期：2001-07-01 00:00:00

abstract：:To facilitate deciphering the information content in the glycome, thin film-coated photoactivatable surfaces were applied for covalent immobilization of glycans, glycoconjugates, or lectins in microarray formats. Light-induced immobilization of a series of bacterial exopolysaccharides on photoactivatable dextran-coate...

journal_title：Glycobiology

authors： Angeloni S,Ridet JL,Kusy N,Gao H,Crevoisier F,Guinchard S,Kochhar S,Sigrist H,Sprenger N

更新日期：2005-01-01 00:00:00

abstract：:Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...

journal_title：Glycobiology

authors： Hara-Kuge S,Seko A,Shimada O,Tosaka-Shimada H,Yamashita K

更新日期：2004-08-01 00:00:00

abstract：:Bacterial protein glycosylation systems from varying species have been functionally reconstituted in Escherichia coli. Both N- and O-linked glycosylation pathways, in which the glycans are first assembled onto lipid carriers and subsequently transferred to acceptor proteins by an oligosaccharyltransferase (OTase), hav...

journal_title：Glycobiology

authors： Gebhart C,Ielmini MV,Reiz B,Price NL,Aas FE,Koomey M,Feldman MF

更新日期：2012-07-01 00:00:00

abstract：:Hyaluronan (HA) is a linear charged polysaccharide whose structure is made up of repeating disaccharide units. Apparently conflicting reports have been published about the nature of the helical structure of HA in the solid state. Recent developments in the field of molecular modeling of polysaccharides offer new oppor...

journal_title：Glycobiology

authors： Haxaire K,Braccini I,Milas M,Rinaudo M,Pérez S

更新日期：2000-06-01 00:00:00

abstract：:Bifidobacteria are predominant in the intestines of breast-fed infants and offer health benefits to the host. Human milk oligosaccharides (HMOs) are considered to be one of the most important growth factors for intestinal bifidobacteria. HMOs contain two major structures of core tetrasaccharide: lacto-N-tetraose (Galβ...

journal_title：Glycobiology

authors： Miwa M,Horimoto T,Kiyohara M,Katayama T,Kitaoka M,Ashida H,Yamamoto K

更新日期：2010-11-01 00:00:00

abstract：:We investigated the localization of major gangliosides in adult rat brain by an immunofluorescence technique with mouse monoclonal antibodies (MAbs). Five MAbs (GMB16, GMR17, GGR12, GMR5 and GMR13) that specifically recognize gangliosides GM1, GD1a, GD1b, GT1b and GQ1b, respectively, were used. We have found that ther...

journal_title：Glycobiology

authors： Kotani M,Kawashima I,Ozawa H,Terashima T,Tai T

更新日期：1993-04-01 00:00:00

abstract：:N-acetylneuraminic acid (5-acetamido-3,5-dideoxy-d-glycero-d-galacto-non-2-ulosonic acid), which is the principal sialic acid family member of the non-2-ulosonic acids and their various derivatives, is often found at the terminal position on the glycan chains that adorn all vertebrate cells. This terminal position com...

journal_title：Glycobiology

authors： Shamsi Kazem Abadi S,Deen MC,Watson JN,Shidmoossavee FS,Bennet AJ

更新日期：2020-04-20 00:00:00

abstract：:The rat liver sinusoidal endothelial cell (LEC) hyaluronan (HA) receptor was previously identified using a photoaffinity HA derivative (J. Biol. Chem., 267, 20451-20456, 1992). Two polypeptides with M(r) = 175,000 and 166,000, were consistently crosslinked, suggesting that the LEC HA receptor is an oligomer. Whether o...

journal_title：Glycobiology

authors： Yannariello-Brown J,Zhou B,Weigel PH

更新日期：1997-02-01 00:00:00

abstract：:Influenza C virus spike glycoprotein HEF specifically recognizes glycoconjugates containing 9-O-acetyl-N-acetylneuraminic acid. The same protein also contains an esterase activity. Taking advantage of these two properties, influenza C virus was used as a very sensitive probe for the detection of traces of 9-O-acetyl-N...

journal_title：Glycobiology

authors： Zimmer G,Suguri T,Reuter G,Yu RK,Schauer R,Herrler G

更新日期：1994-06-01 00:00:00

abstract：:Sialidases are widely distributed glycohydrolytic enzymes removing sialic acid residues from glycoconjugates. In mammals, several sialidases with different subcellular localizations and biochemical features have been described. NEU4, the most recently identified member of the human sialidase family, is found in two fo...

journal_title：Glycobiology

authors： Bigi A,Morosi L,Pozzi C,Forcella M,Tettamanti G,Venerando B,Monti E,Fusi P

更新日期：2010-02-01 00:00:00

abstract：:We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...

journal_title：Glycobiology

authors： Kawabe K,Tateyama D,Toyoda H,Kawasaki N,Hashii N,Nakao H,Matsumoto S,Nonaka M,Matsumura H,Hirose Y,Morita A,Katayama M,Sakuma M,Kawasaki N,Furue MK,Kawasaki T

更新日期：2013-03-01 00:00:00

abstract：:The N-glycans have been removed by peptide-N-glycosidase F (PNGase F) from purified human non-secretory RNases derived from kidney, liver and spleen. The spleen RNase was purified by two procedures, one of which did not include the usual acid treatment step (0.25 M H2SO4, 45 min, 4 degrees C), to determine if acid tre...

journal_title：Glycobiology

authors： Lawrence CW,Little PA,Little BW,Glushka J,van Halbeek H,Alhadeff JA

更新日期：1993-06-01 00:00:00

abstract：:Affinities of the human blood group glycosyltransferases, alpha-(1-->3)-N-acetylgalactosaminyltransferase (GTA) and alpha-(1-->3)-galactosyltransferase (GTB) for their common acceptor substrate alpha-l-Fucp-(1-->2)-beta-d-Galp-O(CH2)(7)CH3 (1), in the absence and presence of bound uridine 5'-diphosphate (UDP) and Mn2+...

journal_title：Glycobiology

authors： Shoemaker GK,Soya N,Palcic MM,Klassen JS

更新日期：2008-08-01 00:00:00

abstract：:Phytohaemagglutinin (PHA) is the major lectin present in the seeds of the common bean, Phaseolus vulgaris, and PHA-L is the leucocyte-agglutinating form of this lectin. This tetrameric glycoprotein accumulates in the vacuoles of storage parenchyma cells. Based on amino acid sequence comparisons of legume lectins and t...

journal_title：Glycobiology

authors： Mirkov TE,Chrispeels MJ

更新日期：1993-12-01 00:00:00

abstract：:alpha-1,4-Glucan lyase cleaves alpha-1,4-linkages of nonreducing termini of alpha-1,4-glucans to produce 1,5-anhydrofructose (1,5-AnFru). The enzymes isolated from fungi and algae show high homology with glycoside hydrolase family 31. Purification of alpha-1,4-glucan lyase from rat liver using DEAE Cellulose chromatog...

journal_title：Glycobiology

authors： Hirano K,Ziak M,Kamoshita K,Sukenaga Y,Kametani S,Shiga Y,Roth J,Akanuma H

更新日期：2000-12-01 00:00:00

abstract：:Mac-2 is a member of the S-(soluble) lectin family. Its identification and isolation from a wide variety of cell types and tissues suggest a diversity of roles in various biological systems. The key points to be made are that Mac-2, and the S-lectins in general, by virtue of their recognition of a variety of carbohydr...

journal_title：Glycobiology

authors： Hughes RC

更新日期：1994-02-01 00:00:00

abstract：:Many mannose-binding proteins inhibit divergent strains of human immunodeficiency virus type 1 (HIV-1) in in vitro models of viral infectivity, suggesting that targeting mannose residues in vaccine applications might offset the strain restriction typically observed in antibody responses to HIV vaccine preparations. Co...

journal_title：Glycobiology

authors： Pashov A,MacLeod S,Saha R,Perry M,VanCott TC,Kieber-Emmons T

更新日期：2005-10-01 00:00:00

abstract：:Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structura...

journal_title：Glycobiology

authors： Kudoh A,Okawa Y,Shibata N

更新日期：2015-01-01 00:00:00

abstract：:The synthesis of the common and well-documented Siaalpha 2,6 to Galbeta 1,4GlcNAc structure (Sia6LacNAc) is principally mediated by the sialyltransferase ST6Gal I, which is particularly highly expressed in liver, lactating mammary gland, intestinal epithelia of newborn animals, and B cells. Multiple independent promot...

journal_title：Glycobiology

authors： Appenheimer MM,Huang RY,Chandrasekaran EV,Dalziel M,Hu YP,Soloway PD,Wuensch SA,Matta KL,Lau JT

更新日期：2003-08-01 00:00:00

abstract：:Murine sperm initiate fertilization by binding to the specialized extracellular matrix of their complementary eggs, known as the zona pellucida. On the basis of data reported in this study, mouse sperm also bind to rabbit erythrocytes with higher affinity than they do to murine eggs. This unusual interaction between a...

journal_title：Glycobiology

authors： Sutton-Smith M,Wong NK,Khoo KH,Wu SW,Yu SY,Patankar MS,Easton R,Lattanzio FA,Morris HR,Dell A,Clark GF

更新日期：2007-06-01 00:00:00

abstract：:Galectin-8 (gal-8) is a "tandem-repeat"-type galectin, containing two carbohydrate recognition domains connected by a linker peptide. gal-8 is expressed both in the cytoplasm and nucleus in vascular endothelial cells (ECs) from normal and tumor-associated blood vessels, and in lymphatic endothelial cells. Herein, we d...

journal_title：Glycobiology

authors： Troncoso MF,Ferragut F,Bacigalupo ML,Cárdenas Delgado VM,Nugnes LG,Gentilini L,Laderach D,Wolfenstein-Todel C,Compagno D,Rabinovich GA,Elola MT

更新日期：2014-10-01 00:00:00

abstract：:The egg jellies of sea urchins contain sulfated polysaccharides with unusual structures, composed of linear chains of l-fucose or l-galactose with well-defined repetitive units. The specific pattern of sulfation and the position of the glycosidic bond vary among sulfated polysaccharides from different species. These p...

journal_title：Glycobiology

authors： Cinelli LP,Castro MO,Santos LL,Garcia CR,Vilela-Silva AC,Mourão PA

更新日期：2007-08-01 00:00:00

abstract：:The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...

journal_title：Glycobiology

authors： Hofinger ES,Spickenreither M,Oschmann J,Bernhardt G,Rudolph R,Buschauer A

更新日期：2007-04-01 00:00:00