Temperature-dependent cooperativity in donor-acceptor substrate binding to the human blood group glycosyltransferases.

abstract：:We have developed a new method to co-localize multiple glycosyl transferases in different Golgi compartments. The approach relies on the proven ability of intact, sealed rat liver Golgi preparations to concentrate exogenous labeled sugar nucleotides into the lumen where they glycosylate either endogenous or artificial...

journal_title：Glycobiology

authors： Etchison JR,Freeze HH

更新日期：1996-03-01 00:00:00

abstract：:Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...

journal_title：Glycobiology

authors： Gerwig GJ,van Kuik JA,Leeflang BR,Kamerling JP,Vliegenthart JF,Karr CD,Jarroll EL

更新日期：2002-08-01 00:00:00

abstract：:Glycoside hydrolases are particularly abundant in all areas of metabolism as they are involved in the degradation of natural polysaccharides and glycoconjugates. These enzymes are classified into 133 families (CAZy server, http://www.cazy.org) in which members of each family have a similar structure and catalytic mech...

journal_title：Glycobiology

authors： André-Miral C,Koné FM,Solleux C,Grandjean C,Dion M,Tran V,Tellier C

更新日期：2015-04-01 00:00:00

abstract：:The human intestine houses a dense microbial ecosystem in which the struggle for nutrients creates a continual and dynamic selective force. Host-produced mucus glycans provide a ubiquitous source of carbon and energy for microbial species. Not surprisingly, many gut resident bacteria have become highly adapted to effi...

journal_title：Glycobiology

authors： Marcobal A,Southwick AM,Earle KA,Sonnenburg JL

更新日期：2013-09-01 00:00:00

abstract：:The egg jellies of sea urchins contain sulfated polysaccharides with unusual structures, composed of linear chains of l-fucose or l-galactose with well-defined repetitive units. The specific pattern of sulfation and the position of the glycosidic bond vary among sulfated polysaccharides from different species. These p...

journal_title：Glycobiology

authors： Cinelli LP,Castro MO,Santos LL,Garcia CR,Vilela-Silva AC,Mourão PA

更新日期：2007-08-01 00:00:00

abstract：:The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transf...

journal_title：Glycobiology

authors： Chan NW,Stangier K,Sherburne R,Taylor DE,Zhang Y,Dovichi NJ,Palcic MM

更新日期：1995-10-01 00:00:00

abstract：:Heparan sulfate is a polysaccharide belonging to the glycaminoglycan family. It interacts with numerous proteins of the extracellular matrix, in particular cellular growth factors. The number of experimental protein-heparin sulfate complexes obtained by crystallography or nuclear magnetic resonance is limited. Alterna...

journal_title：Glycobiology

authors： Sapay N,Cabannes E,Petitou M,Imberty A

更新日期：2011-09-01 00:00:00

abstract：:A glycosyltransferase was fused to the yeast cell wall protein Pir, which forms the Pir1-4 protein family and is incorporated into the cell wall by an unknown linkage to be displayed at the yeast cell surface. We first expressed the PIR1-HA-gma12+ fusion, in which gma12+ encodes alpha-1,2-galactosyltransferase from th...

journal_title：Glycobiology

authors： Abe H,Shimma Y,Jigami Y

更新日期：2003-02-01 00:00:00

abstract：:The occurrence of a terminal disialyl motif on mammalian O-glycans is increasingly being identified through recent mass spectrometry (MS)-based glycomic profiling. In most cases, it is carried on simple core 1 structures in which both the galactose and N-acetyl galactosamine can be disialylated. In contrast, a disialy...

journal_title：Glycobiology

authors： Wang SH,Tsai CM,Lin KI,Khoo KH

更新日期：2013-06-01 00:00:00

abstract：:We show here that cell surface glycosaminoglycans (GAGs) are involved in the binding of stromal cell-derived factor (SDF)-1alpha to CD4(+)lymphoid CEM or monocytic U937 cells, inasmuch as pretreating the cells with heparitinase or chondroitinase inhibits SDF-1alpha binding by 40-41% and 31-35%, respectively. Soluble h...

journal_title：Glycobiology

authors： Mbemba E,Gluckman JC,Gattegno L

更新日期：2000-01-01 00:00:00

abstract：:The content of sulfated glycans having 6-O-sulfated GlcNAc residues alters in the course of colonic carcinogenesis. We previously characterized two GlcNAc 6-O-sulfotransferases (SulTs), SulT-a and -b, expressed in colonic normal tissues and adenocarcinomas [Seko et al. (2000) Glycobiology, 10, 919-929]. Levels of the ...

journal_title：Glycobiology

authors： Seko A,Nagata K,Yonezawa S,Yamashita K

更新日期：2002-06-01 00:00:00

abstract：:Porcine intestinal mucosal heparan sulfate was exhaustively depolymerized on a large scale using heparin lyase II (heparinase II) or heparin lyase III (heparitinase, EC 4.2.2.8). The oligosaccharide mixtures formed with each enzyme were fractionated by low pressure gel permeation chromatography. Size-uniform mixtures ...

journal_title：Glycobiology

authors： Hileman RE,Smith AE,Toida T,Linhardt RJ

更新日期：1997-03-01 00:00:00

abstract：:Glycosaminoglycan (GAG) assembly initiates through the formation of a linkage tetrasaccharide region serving as a primer for both chondroitin sulfate (CS) and heparan sulfate (HS) chain polymerization. A possible role for sulfation of the linkage structure and of the constitutive disaccharide unit of CS chains in the ...

journal_title：Glycobiology

authors： Gulberti S,Jacquinet JC,Chabel M,Ramalanjaona N,Magdalou J,Netter P,Coughtrie MW,Ouzzine M,Fournel-Gigleux S

更新日期：2012-04-01 00:00:00

abstract：:The oligosaccharide chains (glycans) attached to cell surface and extracellular proteins and lipids are known to mediate many important biological roles. However, for many glycans, there are still no evident functions that are of obvious benefit to the organism that synthesizes them. There is also no clear explanation...

journal_title：Glycobiology

authors： Gagneux P,Varki A

更新日期：1999-08-01 00:00:00

abstract：:The transplantation of organs from lower animals such as pigs into humans is prevented by a severe rejection reaction initiated by complement fixing xenoreactive natural antibodies. Most anti-pig xenoreactive natural antibodies in humans are thought to recognize Gal alpha 1-3Gal beta 1-4GlcNAc and are also thought to ...

journal_title：Glycobiology

authors： Parker W,Lateef J,Everett ML,Platt JL

更新日期：1996-07-01 00:00:00

abstract：:Viscumin of mistletoe (Viscum album L.) has a concentration-dependent activity profile unique to plant AB-toxins. It starts with lectin-dependent mitogenicity and then covers toxicity and cell agglutination, associated with shifts in the monomer/dimer equilibrium. Each lectin subunit harbors two sections for ligand co...

journal_title：Glycobiology

authors： Jiménez M,André S,Siebert HC,Gabius HJ,Solís D

更新日期：2006-10-01 00:00:00

abstract：:Affinity-purified glycopeptides were prepared from Trypanosoma cruzi using the carbohydrate-specific monoclonal antibody WIC29.26. These glycopeptides contain rhamnose, fucose, xylose, and galactose, in the ratio 1:1:2:3. A series of oligosaccharides was released from the glycopeptides by mild acid hydrolysis, while, ...

journal_title：Glycobiology

authors： Haynes PA,Ferguson MA,Cross GA

更新日期：1996-12-01 00:00:00

abstract：:Blood dendritic cell antigen 2 (BDCA-2) is a C-type lectin found on the surface of plasmacytoid dendritic cells. It functions as a glycan-binding receptor that downregulates the production of type I interferons and thus plays a role in oligosaccharide-mediated immunomodulation. The carbohydrate recognition domain in B...

journal_title：Glycobiology

authors： Kim JW,Budzak J,Liu Y,Jégouzo SAF,Drickamer K,Taylor ME

更新日期：2018-08-01 00:00:00

abstract：:Cross-reactive carbohydrate determinants of plants are essentially a mixture of N-glycans containing beta1,2-xylose and core alpha1,3-fucose, the latter also found in insect glycoproteins. To determine the relative contributions of these two sugar residues to antibody binding, we prepared an array of glycomodified for...

journal_title：Glycobiology

authors： Bencúrová M,Hemmer W,Focke-Tejkl M,Wilson IB,Altmann F

更新日期：2004-05-01 00:00:00

abstract：:Mouse gene knockout studies have provided unimpeachable evidence of immune-relevant functions for several sialyltransferase enzymes including ST6Gal I, ST3Gal I, and ST3Gal IV. Such studies cannot, however, identify cellular mechanisms for regulating such activities. In this article we provide evidence that murine B l...

journal_title：Glycobiology

authors： Marino JH,Hoffman M,Meyer M,Miller KS

更新日期：2004-12-01 00:00:00

abstract：:Sialyltransferases are a family of 20 gene products in mice and humans that transfer sialic acid from its activated precursor, CMP-sialic acid, to the terminus of glycoprotein and glycolipid acceptors. ST3Gal-II (coded by the St3gal2 gene) transfers sialic acid preferentially to the three positions of galactose on the...

journal_title：Glycobiology

authors： Lopez PH,Aja S,Aoki K,Seldin MM,Lei X,Ronnett GV,Wong GW,Schnaar RL

更新日期：2017-01-01 00:00:00

abstract：:The specificities of glycosaminoglycan (GAG) modification enzymes, particularly sulfotransferases, and the locations and concentrations of these enzymes in the Golgi apparatus give rise to the mature GAG polysaccharides that bind protein ligands. We studied the substrate specificities of sulfotransferases with a stabl...

journal_title：Glycobiology

authors： Shi X,Shao C,Mao Y,Huang Y,Wu ZL,Zaia J

更新日期：2013-08-01 00:00:00

abstract：:Chinese hamster ovary (CHO) cells express only a subset of the glycosyltransferases activities known to exist. They do not express several fucosyltransferases, galactosyltransferases, sialyltransferases or N-acetylglucosaminyltransferases. However, following mutagenesis or transfection with large amounts of DNA, rare ...

journal_title：Glycobiology

authors： Stanley P,Raju TS,Bhaumik M

更新日期：1996-10-01 00:00:00

abstract：:Xanthomonas translucens pv. translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae. The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized. The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2...

journal_title：Glycobiology

authors： Steffens T,Duda K,Lindner B,Vorhölter FJ,Bednarz H,Niehaus K,Holst O

更新日期：2017-03-15 00:00:00

abstract：:N-Acetylglucosaminlyltransferase-V (GnT-V) synthesizes GlcNAcbeta1,6Man branched N-glycans both in vitro and in vivo. A paralog, GnT-Vb (or GnT-IX), has also been shown to synthesize both GlcNAcbeta1,6Man branched N- and O-glycans. GnT-V is expressed in most human and rodent tissues while GnT-Vb expression is limited ...

journal_title：Glycobiology

authors： Alvarez-Manilla G,Troupe K,Fleming M,Martinez-Uribe E,Pierce M

更新日期：2010-02-01 00:00:00

abstract：:A genetic basis for the biosynthetic assembly of the globotetraose containing lipopolysaccharide (LPS) of Haemophilus influenzae strain RM118 (Rd) was determined by structural analysis of LPS derived from mutant strains. We have previously shown that the parent strain RM118 elaborates a population of LPS molecules mad...

journal_title：Glycobiology

authors： Hood DW,Cox AD,Wakarchuk WW,Schur M,Schweda EK,Walsh SL,Deadman ME,Martin A,Moxon ER,Richards JC

更新日期：2001-11-01 00:00:00

abstract：:Heparan sulfate (HS) is a sulfated polysaccharide that plays a key role in morphogenesis, physiology and pathogenesis. The biosynthesis of HS takes place in the Golgi apparatus by a group of enzymes that polymerize, epimerize and sulfate the sugar chain. This biosynthetic process introduces varying degrees of sulfate ...

journal_title：Glycobiology

authors： Gesteira TF,Coulson-Thomas VJ

更新日期：2018-11-01 00:00:00

abstract：:The syp locus includes four genes encoding putative regulators, six genes encoding glycosyltransferases, two encoding export proteins, and six other genes encoding unidentified functional proteins associated with biofilm formation and symbiotic colonization. However, the individual functions of the respective genes re...

journal_title：Glycobiology

authors： Ye L,Zheng X,Zheng H

更新日期：2014-04-01 00:00:00

abstract：:Polypeptide GalNAc-transferases (GalNAc-Ts) constitute a family of 20 human glycosyltransferases (comprising 9 subfamilies), which initiate mucin-type O-glycosylation. The O-glycoproteome is thought to be differentially regulated via the different substrate specificities and expression patterns of each GalNAc-T isofor...

journal_title：Glycobiology

authors： Festari MF,Trajtenberg F,Berois N,Pantano S,Revoredo L,Kong Y,Solari-Saquieres P,Narimatsu Y,Freire T,Bay S,Robello C,Bénard J,Gerken TA,Clausen H,Osinaga E

更新日期：2017-01-01 00:00:00

abstract：:Hypertrophy is central to several heart diseases; however, not much is known about the role of glycosphingolipids (GSLs) in this phenotype. Since GSLs have been accorded several physiological functions, we sought to determine whether these compounds affect cardiac hypertrophy. By using a rat cardiomyoblast cell line, ...

journal_title：Glycobiology

authors： Mishra S,Chatterjee S

更新日期：2014-06-01 00:00:00