Abstract:
:The transplantation of organs from lower animals such as pigs into humans is prevented by a severe rejection reaction initiated by complement fixing xenoreactive natural antibodies. Most anti-pig xenoreactive natural antibodies in humans are thought to recognize Gal alpha 1-3Gal beta 1-4GlcNAc and are also thought to recognize, albeit less avidly, Gal alpha 1-6Glc. Gal alpha 1-6Glc has been used as a ligand for purification of 'anti-Gal alpha 1-3Gal antibodies' and as a therapeutic or reagent to prevent the binding of these antibodies to porcine organs or cells. We tested the specificity of anti-Gal alpha 1-3Gal IgM for Gal alpha 1-6Glc and related saccharides. Based on inhibition of binding of xenoreactive anti-Gal alpha 1-3Gal IgM to porcine cells by soluble saccharides, anti-Gal alpha 1-3Gal IgM in a human serum was found to consist of a mixture of antibodies which have a similar affinity for Gal alpha 1-3Gal but varying affinities for Gal alpha 1-6Glc and other structures. Twenty to 40% of the anti-Gal alpha 1-3Gal IgM from the population tested did not recognize Gal alpha 1-6Glc. The binding of anti-Gal alpha 1-3Gal IgM to Gal alpha 1-6Glc varied widely from individual to individual, some samples lacking almost entirely anti-Gal alpha 1-3Gal IgM which bound to Gal alpha 1-6Glc.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Parker W,Lateef J,Everett ML,Platt JLdoi
10.1093/glycob/6.5.499subject
Has Abstractpub_date
1996-07-01 00:00:00pages
499-506issue
5eissn
0959-6658issn
1460-2423journal_volume
6pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::The galectin from Geodia cydonium (GCA) had previously been shown to be involved in regulatory mechanisms of cell sorting and adhesion during reaggregation of allogeneic sponge cells. In this contribution the binding specificity of GCA was established to be GalNAc alpha 1-3GalNAc beta as structural component of Forssm...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/6.3.321
更新日期:1996-04-01 00:00:00
abstract::Oral treatment of mice, cutaneously infected with herpes simplex virus type 1 (HSV-1) (strain SC16), with the alpha-glucosidase 1 inhibitor 6-O-butanoyl castanospermine (MDL 28,574) produced a significant delay in lesion development and reduced the amount of virus recovered from the brain. Virus load in the brains of ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.2.249
更新日期:1995-03-01 00:00:00
abstract::O-antigen (O-polysaccharide), a part of the outer membrane of Gram-negative bacteria, is one of the most variable cell constituents and is related to bacterial virulence. O-antigen diversity is almost entirely due to genetic variations in O-antigen gene clusters. In this study, the O-polysaccharide structures of Salmo...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq015
更新日期:2010-06-01 00:00:00
abstract::We show here that cell surface glycosaminoglycans (GAGs) are involved in the binding of stromal cell-derived factor (SDF)-1alpha to CD4(+)lymphoid CEM or monocytic U937 cells, inasmuch as pretreating the cells with heparitinase or chondroitinase inhibits SDF-1alpha binding by 40-41% and 31-35%, respectively. Soluble h...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.1.21
更新日期:2000-01-01 00:00:00
abstract::The human anticoagulant factor, Protein C, is a plasma glycoprotein that has reported anti-ischaemic and anti-inflammatory properties. To explore potential mechanisms for these reported activities, we examined the effect of Protein C on the process of cell adhesion to vascular endothelial cells, which plays a critical...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.2.221
更新日期:1994-04-01 00:00:00
abstract::Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.1.37
更新日期:2001-01-01 00:00:00
abstract::Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetyl...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.4.421
更新日期:2000-04-01 00:00:00
abstract::The catabolism of dermatan sulfate (DS) commences with endohydrolysis of the polysaccharide to oligosaccharides by proposed endo-beta-N-acetylhexosaminidase and endohexuronidase activities. To investigate the substrate specificities of these activities, we developed an assay to measure specific products of their actio...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn097
更新日期:2008-12-01 00:00:00
abstract::Changes in mucin protein expression and in glycosylation are common features in pre-neoplastic lesions and cancer and are therefore used as cancer-associated markers. De novo expression of intestinal mucin MUC2 and cancer-associated sialyl-Tn antigen are frequently observed in intestinal metaplasia (IM) and gastric ca...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp161
更新日期:2010-02-01 00:00:00
abstract::The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to design guidelines that improve the reporting and reproducibility of glycoanalytical methods. Previously, the MIRAGE Commission has published gui...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz009
更新日期:2019-05-01 00:00:00
abstract::The oligosaccharide chains (glycans) attached to cell surface and extracellular proteins and lipids are known to mediate many important biological roles. However, for many glycans, there are still no evident functions that are of obvious benefit to the organism that synthesizes them. There is also no clear explanation...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/9.8.747
更新日期:1999-08-01 00:00:00
abstract::CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies), a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, Y0 myeloma and NS0 myeloma cell lines. These engineered cell lines were grown under different culture condit...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.8.813
更新日期:1995-12-01 00:00:00
abstract::It is demonstrated with glycopeptides of the polymorphic epithelial mucin (MUC1) that post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) is a fast, highly sensitive, and reproducible method for the localization of O-glycosylation sites by reflectron time-of-flight (TOF) mass spectrometry. We hav...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.7.881
更新日期:1997-10-01 00:00:00
abstract::The polydispersity of hyaluronan (HA) presents challenges for analyzing its solution properties, such as the relationship between mass and particle size. The broad mass range of natural HA (≤50-fold) makes molecular characterization difficult and ambiguous compared to molecules with known molecular weights (e.g., prot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx039
更新日期:2017-09-01 00:00:00
abstract::Prostate-specific antigen (PSA) is a glycoprotein secreted by prostate epithelial cells. PSA is currently used as a marker of prostate carcinoma because high levels of PSA are indicative of a tumor situation. However, PSA tests still suffer from a lack of specificity to distinguish between benign prostate hyperplasia ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg041
更新日期:2003-06-01 00:00:00
abstract::The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transf...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.7.683
更新日期:1995-10-01 00:00:00
abstract::Heparan sulfate/heparin N-deacetylase/N-sulfotransferase-1 (NDST-1) is a critical enzyme involved in heparan sulfate/heparin biosynthesis. This dual-function enzyme modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan. N-sulfation is an absolute requirement for the subsequent epi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh129
更新日期:2004-12-01 00:00:00
abstract::The structure of the O-antigen polysaccharide (PS) of Escherichia coli O115 has been investigated using a combination of component analysis and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy experiments. The repeating unit of the O-antigen was elucidated using the O-deacetylated PS and has the following branc...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws161
更新日期:2013-03-01 00:00:00
abstract::The glycobiology of Schistosoma mansoni is dominated by developmentally regulated expression of various fucosylated structures, most notably the Lewis X epitope and a multifucosylated sequence, Fuc alpha1-->2Fuc alpha1-->, in its various forms. For the infective cercarial stage, Lewis X has been structurally identifie...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.5.395
更新日期:2001-05-01 00:00:00
abstract::Glycosyltransferases, usually residing within the intracellular secretory apparatus, also circulate in the blood. Many of these blood-borne glycosyltransferases are associated with pathological states, including malignancies and inflammatory conditions. Despite the potential for dynamic modifications of glycans on dis...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww108
更新日期:2017-01-01 00:00:00
abstract::Chinese hamster ovary (CHO) cells express only a subset of the glycosyltransferases activities known to exist. They do not express several fucosyltransferases, galactosyltransferases, sialyltransferases or N-acetylglucosaminyltransferases. However, following mutagenesis or transfection with large amounts of DNA, rare ...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/6.7.695
更新日期:1996-10-01 00:00:00
abstract::N-Acetylglucosaminyltransferase-IV (GnT-IV) has two isoenzymes, GnT-IVa and GnT-IVb, which initiate the GlcNAcbeta1-4 branch synthesis on the Manalpha1-3 arm of the N-glycan core thereby increasing N-glycan branch complexity and conferring endogenous lectin binding epitopes. To elucidate the physiological significance...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp200
更新日期:2010-01-01 00:00:00
abstract::Glycosylation is a common modification found on numerous proteins and lipids. However, direct detection of glycans on these intact biomolecules has been challenge. Here, utilizing enzymatic incorporation of fluorophore-conjugated sialic acids, dubbed as direct fluorescent glycan labeling, we report the labeling and de...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz058
更新日期:2019-10-21 00:00:00
abstract::The syp locus includes four genes encoding putative regulators, six genes encoding glycosyltransferases, two encoding export proteins, and six other genes encoding unidentified functional proteins associated with biofilm formation and symbiotic colonization. However, the individual functions of the respective genes re...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu001
更新日期:2014-04-01 00:00:00
abstract::N-acetylneuraminic acid (5-acetamido-3,5-dideoxy-d-glycero-d-galacto-non-2-ulosonic acid), which is the principal sialic acid family member of the non-2-ulosonic acids and their various derivatives, is often found at the terminal position on the glycan chains that adorn all vertebrate cells. This terminal position com...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz099
更新日期:2020-04-20 00:00:00
abstract::Intelectin is an extracellular animal lectin found in chordata. Although human and mouse intelectin-1 recognize galactofuranosyl residues included in cell walls of various microorganisms, the physiological function of mammalian intelectin had been unclear. In this study, we found that human intelectin-1 was a serum pr...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp013
更新日期:2009-05-01 00:00:00
abstract::Schistosomiasis caused by infection with parasitic helminths of Schistosoma spp. is a major global health problem due to inadequate treatment and lack of a vaccine. The immune response to schistosomes includes glycan antigens, which could be valuable diagnostic markers and vaccine targets. However, no precedent exists...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu027
更新日期:2014-07-01 00:00:00
abstract::The egg jellies of sea urchins contain sulfated polysaccharides with unusual structures, composed of linear chains of l-fucose or l-galactose with well-defined repetitive units. The specific pattern of sulfation and the position of the glycosidic bond vary among sulfated polysaccharides from different species. These p...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm058
更新日期:2007-08-01 00:00:00
abstract::Fucosylation of glycans on glycoproteins and -lipids requires the enzymatic activity of relevant fucosyltransferases and GDP-L-fucose as the donor. Due to the biological importance of fucosylated glycans, a readily accessible source of GDP-L-fucose would be required. Here we describe the construction of a stable recom...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.10.1041
更新日期:2000-10-01 00:00:00
abstract::Sialic acid has long been considered to be the sole receptor for influenza virus. The viral hemagglutinin (HA) is known to bind cell surface sialic acid, and sialic acids on viral glyco-proteins are cleaved by the viral neuraminidase (NA) to promote efficient release of progeny virus particles. However, NWS-Mvi, a mut...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.7.649
更新日期:2000-07-01 00:00:00