Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins.

abstract：:Mycobacterium tuberculosis lipoarabinomannan (LAM) and biosynthetically related lipoglycans and glycans play an important role in host-pathogen interactions. Therefore, the elucidation of the complete biosynthetic pathways of these important molecules is expected to afford novel therapeutic targets. The characterizati...

journal_title：Glycobiology

authors： Larrouy-Maumus G,Gilleron M,Skovierová H,Zuberogoitia S,Brennan PJ,Puzo G,Jackson M,Nigou J

更新日期：2015-11-01 00:00:00

abstract：:Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of th...

journal_title：Glycobiology

authors： Muindi KM,McCarthy PC,Wang T,Vionnet J,Battistel M,Jankowska E,Vann WF

更新日期：2014-02-01 00:00:00

abstract：:Stage-specific embryonic antigen-1 (SSEA-1) is a well-known carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is ...

journal_title：Glycobiology

authors： Yagi H,Yanagisawa M,Kato K,Yu RK

更新日期：2010-08-01 00:00:00

abstract：:Galectins are a sub-family of lectins, defined by their highly conserved beta-sandwich structures and ability to bind to beta-galactosides, like Gal beta1-4 Glc (lactose). Here, we used (15)N-(1)H HSQC and pulse field gradient (PFG) NMR spectroscopy to demonstrate that galectin-1 (gal-1) binds to the relatively large ...

journal_title：Glycobiology

authors： Miller MC,Klyosov A,Mayo KH

更新日期：2009-09-01 00:00:00

abstract：:Since plants are emerging as an important system for the expression of recombinant glycoproteins, especially those intended for therapeutic purposes, it is important to scrutinize to what extent glycans harbored by mammalian glycoproteins produced in transgenic plants differ from their natural counterpart. We report h...

journal_title：Glycobiology

authors： Cabanes-Macheteau M,Fitchette-Lainé AC,Loutelier-Bourhis C,Lange C,Vine ND,Ma JK,Lerouge P,Faye L

更新日期：1999-04-01 00:00:00

abstract：:The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to design guidelines that improve the reporting and reproducibility of glycoanalytical methods. Previously, the MIRAGE Commission has published gui...

journal_title：Glycobiology

authors： Campbell MP,Abrahams JL,Rapp E,Struwe WB,Costello CE,Novotny M,Ranzinger R,York WS,Kolarich D,Rudd PM,Kettner C

更新日期：2019-05-01 00:00:00

abstract：:The N-glycans of human serum transferrin produced in Trichopulsia ni cells were analyzed to examine N-linked oligosaccharide processing in insect cells. Metabolic radiolabeling of the intra- and extracellular protein fractions revealed the presence of multiple transferrin glycoforms with molecular weights lower than t...

journal_title：Glycobiology

authors： Ailor E,Takahashi N,Tsukamoto Y,Masuda K,Rahman BA,Jarvis DL,Lee YC,Betenbaugh MJ

更新日期：2000-08-01 00:00:00

abstract：:We have examined the time course of the neuronal death and regeneration of rat axotomized hypoglossal nerve with various conditions of the nerve resection, and established a useful system to measure neurotrophic activities of bioactive substances. In this system, neuronal death can be evaluated by counting surviving n...

journal_title：Glycobiology

authors： Itoh M,Fukumoto S,Baba N,Kuga Y,Mizuno A,Furukawa K

更新日期：1999-11-01 00:00:00

abstract：:Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was char...

journal_title：Glycobiology

authors： Pinto MR,Gorin PA,Wait R,Mulloy B,Barreto-Bergter E

更新日期：2005-10-01 00:00:00

abstract：:In this work, we analyzed at high resolution the sugar-binding mode of the recombinant N-terminal ricin-B domain of the hemolytic protein LSLa (LSL(150)) from the mushroom Laetiporus sulphureus and also provide functional in vitro evidence suggesting that, together with its putative receptor-binding role, this module ...

journal_title：Glycobiology

authors： Angulo I,Acebrón I,de las Rivas B,Muñoz R,Rodríguez-Crespo I,Menéndez M,García P,Tateno H,Goldstein IJ,Pérez-Agote B,Mancheño JM

更新日期：2011-10-01 00:00:00

abstract：:Endolysins are bacteriophage enzymes that lyse their bacterial host for phage progeny release. They commonly contain an N-terminal catalytic domain that hydrolyzes bacterial peptidoglycan (PG) and a C-terminal cell wall-binding domain (CBD) that confers enzyme localization to the PG substrate. Two endolysins, phage ly...

journal_title：Glycobiology

authors： Ganguly J,Low LY,Kamal N,Saile E,Forsberg LS,Gutierrez-Sanchez G,Hoffmaster AR,Liddington R,Quinn CP,Carlson RW,Kannenberg EL

更新日期：2013-07-01 00:00:00

abstract：:The human intestine houses a dense microbial ecosystem in which the struggle for nutrients creates a continual and dynamic selective force. Host-produced mucus glycans provide a ubiquitous source of carbon and energy for microbial species. Not surprisingly, many gut resident bacteria have become highly adapted to effi...

journal_title：Glycobiology

authors： Marcobal A,Southwick AM,Earle KA,Sonnenburg JL

更新日期：2013-09-01 00:00:00

abstract：:Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...

journal_title：Glycobiology

authors： Takahashi S,Sasaki T,Manya H,Chiba Y,Yoshida A,Mizuno M,Ishida H,Ito F,Inazu T,Kotani N,Takasaki S,Takeuchi M,Endo T

更新日期：2001-01-01 00:00:00

abstract：:Elevated ammonium concentrations in the medium of cultivated cells have been shown to increase the intracellular levels of uridine-5'-diphospho-N-acetylglucosamine (UDP-GlcNAc) and uridine-5'-diphospho-N-acetylgalactosamine (UDP-GalNAc; Ryll et al., 1994). These sugar nucleotides are substrates for glycosyltransferase...

journal_title：Glycobiology

authors： Gawlitzek M,Papac DI,Sliwkowski MB,Ryll T

更新日期：1999-02-01 00:00:00

abstract：:Sulphated N-linked carbohydrate chains isolated from recombinant human tissue plasminogen activator expressed in mouse epithelial (C127) cells were analysed as oligosaccharide alditols by methylation analysis, liquid secondary ion mass spectrometry, and one- and two-dimensional 1H-NMR spectroscopy. The results demonst...

journal_title：Glycobiology

authors： Pfeiffer G,Stirm S,Geyer R,Strube KH,Bergwerff AA,Kamerling JP,Vliegenthart JF

更新日期：1992-10-01 00:00:00

abstract：:Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene...

journal_title：Glycobiology

authors： Berends E,Lehle L,Henquet M,Hesselink T,Wösten HA,Lugones LG,Bosch D

更新日期：2013-02-01 00:00:00

abstract：:The interaction of heparan sulfate (HS) (and the closely related molecule heparin) with FGF-1 is a requirement for enabling the growth factor to activate its cell surface tyrosine kinase receptor. However, little is known about the regulatory role of naturally occurring cell surface HS in FGF-1 activation. We have add...

journal_title：Glycobiology

authors： Pye DA,Vivès RR,Hyde P,Gallagher JT

更新日期：2000-11-01 00:00:00

abstract：:Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...

journal_title：Glycobiology

authors： Yang JM,Byrd JC,Siddiki BB,Chung YS,Okuno M,Sowa M,Kim YS,Matta KL,Brockhausen I

更新日期：1994-12-01 00:00:00

abstract：:The concanavalin A staining of cellular glycoproteins and the direct analysis of their sugar chains released by hydrazinolysis revealed that the processing of N-linked sugar chains of some glycoproteins is suppressed by exposure of mouse monocytoid cells P388D1 to dimethyl sulphoxide, which can induce Fc receptor-medi...

journal_title：Glycobiology

authors： Fukushima K,Takasaki S

更新日期：1993-02-01 00:00:00

abstract：:Trichomonad species are widespread unicellular flagellated parasites of vertebrates which interact with their hosts through carbohydrate-lectin interactions. In the past, some data have been accumulated regarding their lipo(phospho)glycans, a major glycoconjugate on their cell surfaces; on the other hand, other than b...

journal_title：Glycobiology

authors： Paschinger K,Hykollari A,Razzazi-Fazeli E,Greenwell P,Leitsch D,Walochnik J,Wilson IB

更新日期：2012-02-01 00:00:00

abstract：:Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydrate-binding activity at the cell surface. In contrast, galectins were isolated on the b...

journal_title：Glycobiology

authors： Haudek KC,Patterson RJ,Wang JL

更新日期：2010-10-01 00:00:00

abstract：:Porcine intestinal mucosal heparan sulfate was exhaustively depolymerized on a large scale using heparin lyase II (heparinase II) or heparin lyase III (heparitinase, EC 4.2.2.8). The oligosaccharide mixtures formed with each enzyme were fractionated by low pressure gel permeation chromatography. Size-uniform mixtures ...

journal_title：Glycobiology

authors： Hileman RE,Smith AE,Toida T,Linhardt RJ

更新日期：1997-03-01 00:00:00

abstract：:There is increasing evidence that secretory fluids such as tears, saliva and milk play an important role in protecting the human body from infection via a washing mechanism involving glycan-mediated adhesion of potential pathogens to secretory glycoproteins. Interaction of sweat with bacteria is well established as th...

journal_title：Glycobiology

authors： Peterson RA,Gueniche A,Adam de Beaumais S,Breton L,Dalko-Csiba M,Packer NH

更新日期：2016-03-01 00:00:00

abstract：:Sulfated O-linked oligosaccharides from the sea urchin egg receptor have been shown to bind to acrosome-reacted sperm and to inhibit fertilization in a competitive bioassay. However, the inhibitory activity of these isolated chains was much lower than that of a recombinant protein representing a portion of the extrace...

journal_title：Glycobiology

authors： Dhume ST,Stears RL,Lennarz WJ

更新日期：1996-01-01 00:00:00

abstract：:CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies), a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, Y0 myeloma and NS0 myeloma cell lines. These engineered cell lines were grown under different culture condit...

journal_title：Glycobiology

authors： Lifely MR,Hale C,Boyce S,Keen MJ,Phillips J

更新日期：1995-12-01 00:00:00

abstract：:Cytotrophoblasts are the specialized epithelial cells of the placenta. During the first trimester, a subpopulation of chorionic villus cytotrophoblasts differentiates along an invasive pathway and penetrates the maternal endometrium, decidua and spiral arterioles. Cytotrophoblast invasiveness declines rapidly during t...

journal_title：Glycobiology

authors： Moss L,Prakobphol A,Wiedmann TW,Fisher SJ,Damsky CH

更新日期：1994-10-01 00:00:00

abstract：:Prostate-specific antigen (PSA) is a glycoprotein secreted by prostate epithelial cells. PSA is currently used as a marker of prostate carcinoma because high levels of PSA are indicative of a tumor situation. However, PSA tests still suffer from a lack of specificity to distinguish between benign prostate hyperplasia ...

journal_title：Glycobiology

authors： Peracaula R,Tabarés G,Royle L,Harvey DJ,Dwek RA,Rudd PM,de Llorens R

更新日期：2003-06-01 00:00:00

abstract：:Lectins are used as defense effector proteins against predators, parasites and pathogens by animal, plant and fungal innate defense systems. These proteins bind to specific glycoepitopes on the cell surfaces and thereby interfere with the proper cellular functions of the various antagonists. The exact cellular toxicit...

journal_title：Glycobiology

authors： Bleuler-Martinez S,Stutz K,Sieber R,Collot M,Mallet JM,Hengartner M,Schubert M,Varrot A,Künzler M

更新日期：2017-05-01 00:00:00

abstract：:The catabolism of dermatan sulfate (DS) commences with endohydrolysis of the polysaccharide to oligosaccharides by proposed endo-beta-N-acetylhexosaminidase and endohexuronidase activities. To investigate the substrate specificities of these activities, we developed an assay to measure specific products of their actio...

journal_title：Glycobiology

authors： Nielsen TC,Meikle PJ,Hopwood JJ,Fuller M

更新日期：2008-12-01 00:00:00

abstract：:Cryptococcus neoformans, an opportunistic fungal pathogen, produces a glycan capsule to evade the immune system during infection. This definitive virulence factor is composed mainly of complex polysaccharides, which are made in the secretory pathway by reactions that utilize activated nucleotide sugar precursors. Alth...

journal_title：Glycobiology

authors： Li LX,Ashikov A,Liu H,Griffith CL,Bakker H,Doering TL

更新日期：2017-01-01 00:00:00