Abstract:
:Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was characterized by a combination of techniques including electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI MS/MS), matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), nuclear magnetic resonance (NMR), and methylation gas-liquid chromatography-mass spectrometry (GC-MS) analysis. It was branched, with a principal chain of alpha-Rhap-(1 --> 3)-alpha-Rhap-(1 --> 3)-alpha-Manp-(1 --> 2)-Man-ol substituted at O-6 of mannitol with an alpha-Glcp-(1 --> 4)-beta-Galp group. Species containing one and two additional alpha-Glcp-(1 --> 4) substituents in the rhamnose branch were also present. The major component of fraction 2 was a substructure of oligosaccharide-1, lacking a hexose from the Glc-Gal branch. Fraction 3 contained a mixture of smaller, unbranched, oligosaccharides. In hapten inhibition tests, fractions 1 and 2 blocked the reaction between peptidorhamnomannan (PRM) and rabbit anti-P. boydii mycelium hyperimmune serum by approximately 75%, whereas fraction 3 inhibited by approximately 55%.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Pinto MR,Gorin PA,Wait R,Mulloy B,Barreto-Bergter Edoi
10.1093/glycob/cwi084subject
Has Abstractpub_date
2005-10-01 00:00:00pages
895-904issue
10eissn
0959-6658issn
1460-2423pii
cwi084journal_volume
15pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::In Campylobacter jejuni, the PglB oligosaccharyltransferase catalyzes the transfer of a heptasaccharide from a lipid donor to asparagine within the D/E-X1-N-X2-S/T sequon (X1,2 ≠ P) or releases this heptasaccharide as free oligosaccharides (fOS). Using available crystal structures and sequence alignments, we identifie...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx067
更新日期:2017-10-01 00:00:00
abstract::From a systematic search of the UniGene and dbEST databanks, using human beta 4-galactosyltransferase (beta 4GalT-I), which is recognized to function in lactose biosynthesis, as the query sequence, we have identified five additional gene family members denoted as beta 4GalT-II, -III, -IV, -V, and -VI. Complementary DN...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.5.517
更新日期:1998-05-01 00:00:00
abstract::Trichomonad species are widespread unicellular flagellated parasites of vertebrates which interact with their hosts through carbohydrate-lectin interactions. In the past, some data have been accumulated regarding their lipo(phospho)glycans, a major glycoconjugate on their cell surfaces; on the other hand, other than b...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr149
更新日期:2012-02-01 00:00:00
abstract::Galactans are linear polysaccharides of β(1→4)-linked galactose residues. Although they can antagonize galectin function, the nature of their binding to galectins needs to be better defined to develop them as drugs. Here, we investigated interactions between galectin-3 (Gal-3) and a series of galactans ranging in weig...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy085
更新日期:2019-01-01 00:00:00
abstract::N-acetylneuraminic acid (5-acetamido-3,5-dideoxy-d-glycero-d-galacto-non-2-ulosonic acid), which is the principal sialic acid family member of the non-2-ulosonic acids and their various derivatives, is often found at the terminal position on the glycan chains that adorn all vertebrate cells. This terminal position com...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz099
更新日期:2020-04-20 00:00:00
abstract::Asparagine N-linked glycosylation is one of the most important forms of protein post-translational modification in eukaryotes and is one of the first metabolic pathways described at a biochemical level. Here, we report a new annotation of this pathway for the Human species, published after passing a peer-review proces...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq215
更新日期:2011-11-01 00:00:00
abstract::Apolipoprotein C-III (apoCIII) is a small glycoprotein with a single mucin-type core-1 oligosaccharide and is analyzed by isoelectric focusing (IEF) for the diagnosis of genetic defects in O-glycan biosynthesis such as congenital disorders of glycosylation. In the present study, mass spectrometry of apoCIII, after a s...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws086
更新日期:2012-08-01 00:00:00
abstract::Presently almost no information is available on the oligosaccharide structure of the glycoproteins secreted from the methylotrophic yeast Hansenula polymorpha, a promising host for the production of recombinant proteins. In this study, we analyze the size distribution and structure of N-linked oligosaccharides attache...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh030
更新日期:2004-03-01 00:00:00
abstract::Blood dendritic cell antigen 2 (BDCA-2) is a C-type lectin found on the surface of plasmacytoid dendritic cells. It functions as a glycan-binding receptor that downregulates the production of type I interferons and thus plays a role in oligosaccharide-mediated immunomodulation. The carbohydrate recognition domain in B...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy050
更新日期:2018-08-01 00:00:00
abstract::Cross-reactive carbohydrate determinants of plants are essentially a mixture of N-glycans containing beta1,2-xylose and core alpha1,3-fucose, the latter also found in insect glycoproteins. To determine the relative contributions of these two sugar residues to antibody binding, we prepared an array of glycomodified for...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh058
更新日期:2004-05-01 00:00:00
abstract::A rat pheochromocytoma cell line (PC12), when transfected with beta1,4-N-acetylglucosaminyltransferase III (GnT-III), which catalyzes the formation of a bisecting GlcNAc structure in N-glycans, resulted in the suppression of neurite outgrowth induced by costimulation of epidermal growth factor (EGF) and integrins. The...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh016
更新日期:2004-02-01 00:00:00
abstract::The hexosamine pathway (HP) is a biochemical hypothesis recently proposed explaining cellular alterations occurring during diabetic microvascular complications. Diabetic retinopathy is a common microvascular complication of diabetes, and it is known that cell proliferation is severely affected during the development o...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi039
更新日期:2005-06-01 00:00:00
abstract::Trypanosoma cruzi expresses a unique trans-sialidase that is responsible for the transfer of sialic acid from host glycoproteins and glycolipids to mucin-like glycoprotein acceptors on the parasite surface. The enzyme and the sialic acid acceptors are present in the mammalian forms of the parasite and in the parasite ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.6.625
更新日期:1995-09-01 00:00:00
abstract::The polydispersity of hyaluronan (HA) presents challenges for analyzing its solution properties, such as the relationship between mass and particle size. The broad mass range of natural HA (≤50-fold) makes molecular characterization difficult and ambiguous compared to molecules with known molecular weights (e.g., prot...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx039
更新日期:2017-09-01 00:00:00
abstract::Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz061
更新日期:2019-11-20 00:00:00
abstract::The acid alpha-mannosidase of Trypanosoma cruzi is a broad-specificity hydrolase involved in the catabolism of glycoconjugates, presumably in the digestive vacuole. We have cloned the alpha-mannosidase gene from a T.cruzi epimastigote genomic library. The alpha-mannosidase gene was determined to be single copy by Sout...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.12.1183
更新日期:1998-12-01 00:00:00
abstract::N-Glycanase 1, encoded by NGLY1, catalyzes the deglycosylation of misfolded N-linked glycoproteins retrotranslocated into the cytosol. We identified nine cases with mutations in NGLY1. The patients show developmental delay, seizures, peripheral neuropathy, abnormal liver function and alacrima (absence of tears). The m...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwv024
更新日期:2015-08-01 00:00:00
abstract::Porcine intestinal mucosal heparan sulfate was exhaustively depolymerized on a large scale using heparin lyase II (heparinase II) or heparin lyase III (heparitinase, EC 4.2.2.8). The oligosaccharide mixtures formed with each enzyme were fractionated by low pressure gel permeation chromatography. Size-uniform mixtures ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.2.231
更新日期:1997-03-01 00:00:00
abstract::The cDNAs that encode the 70 kDa C-terminal portion of Plasmodium falciparum merozoite surface protein 1 (MSP-1), with or without an N-terminal signal peptide sequence and C-terminal glycosylphosphatidylinositol (GPI) signal sequence of MSP-1, were expressed in mammalian cell lines via recombinant vaccinia virus. The ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.12.1347
更新日期:1999-12-01 00:00:00
abstract::A common argument against using plants as a production system for therapeutic proteins is their inability to perform authentic N-glycosylation. A major concern is the presence of beta 1,2-xylose and core alpha 1,3-fucose residues on complex N-glycans as these nonmammalian N-glycan residues may provoke unwanted side ef...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm137
更新日期:2008-03-01 00:00:00
abstract::Heparan sulfate/heparin N-deacetylase/N-sulfotransferase-1 (NDST-1) is a critical enzyme involved in heparan sulfate/heparin biosynthesis. This dual-function enzyme modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan. N-sulfation is an absolute requirement for the subsequent epi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh129
更新日期:2004-12-01 00:00:00
abstract::Lipid-A represents the ubiquitous, covalently bound hydrophobic component of bacterial lipopolysaccharides (endotoxins). Lipid-As isolated and characterized from rhizobial species have large variations in their backbone sugars, as well as in their hydroxy fatty acid substituents. The sugar backbones consist of either ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.6.535
更新日期:1992-12-01 00:00:00
abstract::Heparan sulfate (HS) is a sulfated polysaccharide that plays a key role in morphogenesis, physiology and pathogenesis. The biosynthesis of HS takes place in the Golgi apparatus by a group of enzymes that polymerize, epimerize and sulfate the sugar chain. This biosynthetic process introduces varying degrees of sulfate ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy055
更新日期:2018-11-01 00:00:00
abstract::Lymphocytic beta 1,4-galactosyltransferase (beta 1,4-GalTase, EC 2.4.1.38) activity was measured in B cells using a neoglyco-protein, N-acetylglucosamine-phenylisothiocyanate-bovine serum albumin (GlcNAc-pITC-BSA), as an acceptor substrate in a novel enzyme-linked immunosorbent assay (ELISA)-based method. This assay p...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.4.365
更新日期:1995-06-01 00:00:00
abstract::We developed a new in vitro assay for oligosaccharyltransferase (OST), which catalyzes the transfer of preassembled oligosaccharides on lipid carriers onto asparagine residues in polypeptide chains. The asparagine residues reside in the sequon, Asn-X-Thr/Ser, where X can be any amino acid residue except Pro. We demons...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm087
更新日期:2007-11-01 00:00:00
abstract::Affinities of the human blood group glycosyltransferases, alpha-(1-->3)-N-acetylgalactosaminyltransferase (GTA) and alpha-(1-->3)-galactosyltransferase (GTB) for their common acceptor substrate alpha-l-Fucp-(1-->2)-beta-d-Galp-O(CH2)(7)CH3 (1), in the absence and presence of bound uridine 5'-diphosphate (UDP) and Mn2+...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwn043
更新日期:2008-08-01 00:00:00
abstract::There has been a recent explosion in the identification of neuromuscular diseases caused by mutations in genes that affect carbohydrate metabolism or protein glycosylation. A number of these findings relate to defects in the glycosylation of alpha dystroglycan. Alpha dystroglycan is an essential component of the dystr...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwg077
更新日期:2003-08-01 00:00:00
abstract::We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws159
更新日期:2013-03-01 00:00:00
abstract::We have elucidated the carbohydrate-binding profile of a non-monosaccharide-binding lectin named Eucheuma serra lectin (ESA)-2 from the red alga Eucheuma serra using a lectin-immobilized column and a centrifugal ultrafiltration-high performance liquid chromatography method with a variety of fluorescence-labeled oligos...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm007
更新日期:2007-05-01 00:00:00
abstract::The glycobiology of Schistosoma mansoni is dominated by developmentally regulated expression of various fucosylated structures, most notably the Lewis X epitope and a multifucosylated sequence, Fuc alpha1-->2Fuc alpha1-->, in its various forms. For the infective cercarial stage, Lewis X has been structurally identifie...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.5.395
更新日期:2001-05-01 00:00:00