Production of N-sulfated polysaccharides using yeast-expressed N-deacetylase/N-sulfotransferase-1 (NDST-1).

abstract：:Isolation of glycopeptides utilizing hydrogen bonding between glycopeptide glycans and a carbohydrate-gel matrix in the organic phase is useful for site-specific characterization of oligosaccharides of glycoproteins, when combined with mass spectrometry. In this study, recovery of glycopeptides was improved by includi...

journal_title：Glycobiology

authors： Tajiri M,Yoshida S,Wada Y

更新日期：2005-12-01 00:00:00

abstract：:Apolipoprotein C-III (apoCIII) is a small glycoprotein with a single mucin-type core-1 oligosaccharide and is analyzed by isoelectric focusing (IEF) for the diagnosis of genetic defects in O-glycan biosynthesis such as congenital disorders of glycosylation. In the present study, mass spectrometry of apoCIII, after a s...

journal_title：Glycobiology

authors： Wada Y,Kadoya M,Okamoto N

更新日期：2012-08-01 00:00:00

abstract：:The structure of a antigen-binding fragment (Fab) from the bactericidal monoclonal antibody LPT3-1 specific to lipooligosaccharide (LOS) inner cores from Neisseria meningitidis has been solved in complex with an eight-sugar inner core fragment NmL3 galE lpt3 KOH to 2.69 Å resolution. The epitope is centered about an i...

journal_title：Glycobiology

authors： Parker MJ,Gomery K,Richard G,MacKenzie CR,Cox AD,Richards JC,Evans SV

更新日期：2014-05-01 00:00:00

abstract：:Oral treatment of mice, cutaneously infected with herpes simplex virus type 1 (HSV-1) (strain SC16), with the alpha-glucosidase 1 inhibitor 6-O-butanoyl castanospermine (MDL 28,574) produced a significant delay in lesion development and reduced the amount of virus recovered from the brain. Virus load in the brains of ...

journal_title：Glycobiology

authors： Bridges CG,Ahmed SP,Kang MS,Nash RJ,Porter EA,Tyms AS

更新日期：1995-03-01 00:00:00

abstract：:Glycosyltransferases, usually residing within the intracellular secretory apparatus, also circulate in the blood. Many of these blood-borne glycosyltransferases are associated with pathological states, including malignancies and inflammatory conditions. Despite the potential for dynamic modifications of glycans on dis...

journal_title：Glycobiology

authors： Lee-Sundlov MM,Ashline DJ,Hanneman AJ,Grozovsky R,Reinhold VN,Hoffmeister KM,Lau JT

更新日期：2017-01-01 00:00:00

abstract：:Gangliosides (sialic acid-containing glycosphingolipids) play important roles in many physiological functions, including synaptic plasticity in the hippocampus, which is considered as a cellular mechanism of learning and memory. In the present study, three types of synaptic plasticity, long-term potentiation (LTP), lo...

journal_title：Glycobiology

authors： Ikarashi K,Fujiwara H,Yamazaki Y,Goto J,Kaneko K,Kato H,Fujii S,Sasaki H,Fukumoto S,Furukawa K,Waki H,Furukawa K

更新日期：2011-10-01 00:00:00

abstract：:Schistosomiasis caused by infection with parasitic helminths of Schistosoma spp. is a major global health problem due to inadequate treatment and lack of a vaccine. The immune response to schistosomes includes glycan antigens, which could be valuable diagnostic markers and vaccine targets. However, no precedent exists...

journal_title：Glycobiology

authors： Prasanphanich NS,Luyai AE,Song X,Heimburg-Molinaro J,Mandalasi M,Mickum M,Smith DF,Nyame AK,Cummings RD

更新日期：2014-07-01 00:00:00

abstract：:At weaning, the intestinal mucosa surface glycans change from predominantly sialylated to fucosylated. Intestinal adaptation from milk to solid food is regulated by intrinsic and extrinsic factors. The contribution by glucocorticoid, an intrinsic factor, and colonization by microbiota, an extrinsic factor, was measure...

journal_title：Glycobiology

authors： Nanthakumar NN,Meng D,Newburg DS

更新日期：2013-10-01 00:00:00

abstract：:Galectins are a family of carbohydrate-binding proteins with an affinity for beta-galactosides. Galectin-1 (Gal-1) is differentially expressed by various normal and pathological tissues and appears to be functionally polyvalent, with a wide range of biological activity. The intracellular and extracellular activity of ...

journal_title：Glycobiology

authors： Camby I,Le Mercier M,Lefranc F,Kiss R

更新日期：2006-11-01 00:00:00

abstract：:Sialyltransferases are a family of 20 gene products in mice and humans that transfer sialic acid from its activated precursor, CMP-sialic acid, to the terminus of glycoprotein and glycolipid acceptors. ST3Gal-II (coded by the St3gal2 gene) transfers sialic acid preferentially to the three positions of galactose on the...

journal_title：Glycobiology

authors： Lopez PH,Aja S,Aoki K,Seldin MM,Lei X,Ronnett GV,Wong GW,Schnaar RL

更新日期：2017-01-01 00:00:00

abstract：:Invariant natural killer T (iNKT) cells recognize glycolipid antigens bound to CD1d molecules on antigen-presenting cells. Therapeutic activation of iNKT cells with the xenogeneic glycolipid α-galactosylceramide (α-GalCer) can prevent and reverse tumor growth in murine models, but clinical trials using α-GalCer-stimul...

journal_title：Glycobiology

authors： Melo AM,Zhang L,Dockry ÉF,Petrasca A,Ghnewa YG,Breen EP,Morrissey ME,O'Reilly C,Bruen R,O'Meara A,Lysaght J,Zhu X,Doherty DG

更新日期：2018-07-01 00:00:00

abstract：:Vesicular integral protein of 36 kDa (VIP36) is an intracellular lectin recognizing high-mannose type glycans and is highly expressed in salivary glands, especially the parotid gland, which secretes alpha-amylase in large quantities. Here immunoelectron microscopy demonstrated that VIP36 was primarily localized to sec...

journal_title：Glycobiology

authors： Hara-Kuge S,Seko A,Shimada O,Tosaka-Shimada H,Yamashita K

更新日期：2004-08-01 00:00:00

abstract：:Sequestration of Plasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin 4-sulfate (C4S). A cytoadherence assay using chondroitin sulfate proteoglycans (CSPGs) is widely used for studying C4S-IRBC interactions. Bovine tracheal chondroitin sulfate A (CSA) preparation lack...

journal_title：Glycobiology

authors： Muthusamy A,Achur RN,Valiyaveettil M,Madhunapantula SV,Kakizaki I,Bhavanandan VP,Gowda CD

更新日期：2004-07-01 00:00:00

abstract：:We report here a method of simultaneously quantifying glucosylceramide (GlcCer) and galactosylceramide (GalCer) by normal-phase HPLC using O-phtalaldehyde derivatives. Treatment with sphingolipid ceramide N-deacylase which converts the cerebrosides in the sample to their lyso-forms was followed by the quantitative lab...

journal_title：Glycobiology

authors： Zama K,Hayashi Y,Ito S,Hirabayashi Y,Inoue T,Ohno K,Okino N,Ito M

更新日期：2009-07-01 00:00:00

abstract：:Protein N- and O-glycosylation are well known co- and post-translational modifications of immunoglobulins. Antibody glycosylation on the Fab and Fc portion is known to influence antigen binding and effector functions, respectively. To study associations between antibody glycosylation profiles and (patho) physiological...

journal_title：Glycobiology

authors： de Haan N,Falck D,Wuhrer M

更新日期：2020-03-20 00:00:00

abstract：:Mouse macrophage galactose/N-acetylgalactosamine-specific C-type lectin (MMGL) is a type II transmembrane glycoprotein belonging to the C-type lectin family. Our development of monoclonal antibodies led us to discover that a calcium-dependent conformational change is detected by an antibody (termed mAb LOM-11) and tha...

journal_title：Glycobiology

authors： Hosoi T,Imai Y,Irimura T

更新日期：1998-08-01 00:00:00

abstract：:Assembly of a protective cyst wall by Giardia is essential for the survival of the parasite outside the host intestine and for transmission among susceptible hosts. The structure of the G. intestinalis filamentous cyst wall was studied by chemical methods, mass spectrometry, and (1)H nuclear magnetic resonance spectro...

journal_title：Glycobiology

authors： Gerwig GJ,van Kuik JA,Leeflang BR,Kamerling JP,Vliegenthart JF,Karr CD,Jarroll EL

更新日期：2002-08-01 00:00:00

abstract：:Lipid-A represents the ubiquitous, covalently bound hydrophobic component of bacterial lipopolysaccharides (endotoxins). Lipid-As isolated and characterized from rhizobial species have large variations in their backbone sugars, as well as in their hydroxy fatty acid substituents. The sugar backbones consist of either ...

journal_title：Glycobiology

authors： Bhat UR,Carlson RW

更新日期：1992-12-01 00:00:00

abstract：:Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...

journal_title：Glycobiology

authors： Guarino C,DeLisa MP

更新日期：2012-05-01 00:00:00

abstract：:Mechanisms for nonenzymatic protein glycation have been extensively studied albeit with an emphasis at the later stages that gives rise to advanced glycation end products. No detailed investigation of the initial, noncovalent binding of d-glucose to human hemoglobin A (HbA) exists in the literature. Although anionic m...

journal_title：Glycobiology

authors： Clark SL,Santin AE,Bryant PA,Holman R,Rodnick KJ

更新日期：2013-11-01 00:00:00

abstract：:Glycosylation determines essential biological functions of epithelial mucins in health and disease. We report on the influence of glycosylation of the immunodominant DTR motif of MUC1 on its antigenicity. Sets of novel glycopeptides were synthesized that enabled us to examine sole and combined effects of peptide lengt...

journal_title：Glycobiology

authors： Karsten U,Serttas N,Paulsen H,Danielczyk A,Goletz S

更新日期：2004-08-01 00:00:00

abstract：:A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes...

journal_title：Glycobiology

authors： Nimrichter L,Gargir A,Gortler M,Altstock RT,Shtevi A,Weisshaus O,Fire E,Dotan N,Schnaar RL

更新日期：2004-02-01 00:00:00

abstract：:The transcriptional regulation mechanisms involved in the up-regulation of Fas-induced GD3 synthase gene have not yet been elucidated. 5'-Rapid amplification of cDNA end (5'-RACE) using mRNA prepared from Fas-induced Jurkat T cells revealed the presence of multiple transcription start sites of human GD3 synthase gene,...

journal_title：Glycobiology

authors： Kang NY,Kang SK,Lee YC,Choi HJ,Lee YS,Cho SY,Kim YS,Ko JH,Kim CH

更新日期：2006-05-01 00:00:00

abstract：:Glycosylation is the most common post-translational modification of serum proteins, and changes in the type and abundance of glycans in human serum have been correlated with a growing number of human diseases. While the glycosylation pattern of human serum is well studied, little is known about the profiles of other m...

journal_title：Glycobiology

authors： Behrens AJ,Duke RM,Petralia LM,Harvey DJ,Lehoux S,Magnelli PE,Taron CH,Foster JM

更新日期：2018-11-01 00:00:00

abstract：:beta1-6 GlcNAc branching, a product of N-acetylglucosaminyltransferase V (GnT-V), is a key structure that is associated with malignant transformations and cancer metastasis. Although a number of reports concerning tumor metastasis-related glycoproteins that contain beta1-6 GlcNAc branching have appeared, the precise f...

journal_title：Glycobiology

authors： Ihara S,Miyoshi E,Nakahara S,Sakiyama H,Ihara H,Akinaga A,Honke K,Dickson RB,Lin CY,Taniguchi N

更新日期：2004-02-01 00:00:00

abstract：:Differences in the fertilization behavior of Xenopus borealis from X. laevis and X. tropicalis suggest differences in the glycosylation of the egg jellies. To test this assumption, O-linked glycans were chemically released from the egg jelly coat glycoproteins of X. borealis. Over 50 major neutral glycans were observe...

journal_title：Glycobiology

authors： Li B,Russell SC,Zhang J,Hedrick JL,Lebrilla CB

更新日期：2011-07-01 00:00:00

abstract：:In renal cell carcinoma (RCC), the presence of higher gangliosides correlates with systematic metastasis. Disialosyl globopentaosylceramide (DSGb5) was identified previously as one of the major gangliosides from RCC tissues. Siglec-7 (sialic acid-binding Ig-like lectin-7), expressed on natural killer (NK) cells as an ...

journal_title：Glycobiology

authors： Kawasaki Y,Ito A,Withers DA,Taima T,Kakoi N,Saito S,Arai Y

更新日期：2010-11-01 00:00:00

abstract：:The catabolism of dermatan sulfate (DS) commences with endohydrolysis of the polysaccharide to oligosaccharides by proposed endo-beta-N-acetylhexosaminidase and endohexuronidase activities. To investigate the substrate specificities of these activities, we developed an assay to measure specific products of their actio...

journal_title：Glycobiology

authors： Nielsen TC,Meikle PJ,Hopwood JJ,Fuller M

更新日期：2008-12-01 00:00:00

abstract：:A large family of UDP-GalNAc:polypeptide GalNAc transferases (ppGalNAc-Ts) initiates and defines sites of mucin-type Ser/Thr-O-GalNAc glycosylation. Family members have been classified into peptide- and glycopeptide-preferring subfamilies, although both families possess variable activities against glycopeptide substra...

journal_title：Glycobiology

authors： Revoredo L,Wang S,Bennett EP,Clausen H,Moremen KW,Jarvis DL,Ten Hagen KG,Tabak LA,Gerken TA

更新日期：2016-04-01 00:00:00

abstract：:The substrate specificity of an alpha2,3-sialyltransferase (v-ST3Gal I) obtained from myxoma virus infected RK13 cells has been determined. Like mammalian sialyltransferase enzymes, the viral enzyme contains the characteristic L- and S-sialyl motif sequences in its catalytic domain. Analysis of the deduced amino acid ...

journal_title：Glycobiology

authors： Sujino K,Jackson RJ,Chan NW,Tsuji S,Palcic MM

更新日期：2000-03-01 00:00:00