Addition of beta1-6 GlcNAc branching to the oligosaccharide attached to Asn 772 in the serine protease domain of matriptase plays a pivotal role in its stability and resistance against trypsin.

abstract：:P3 is a mouse monoclonal antibody (mAb) that binds to several NeuGc-containing gangliosides. It also reacts with antigens expressed in human breast tumors (Vázquez et al. (1995) Hybridoma , 14, 551-556). In this work, the binding specificity of P3 has been characterized in more detail using a panel of glycolipids that...

journal_title：Glycobiology

authors： Moreno E,Lanne B,Vázquez AM,Kawashima I,Tai T,Fernández LE,Karlsson KA,Angström J,Pérez R

更新日期：1998-07-01 00:00:00

abstract：:The substrate specificity of the alpha-D-mannosidases of rat liver lysosome and cytosol was examined using oligosaccharides of the oligomannosidic type. The hydrolysis products were characterized by 400 MHz 1H-NMR spectroscopy. Both catabolic pathways occur in ordered ways, but are quite different. In fact, the lysoso...

journal_title：Glycobiology

authors： Haeuw JF,Michalski JC,Strecker G,Spik G,Montreuil J

更新日期：1991-11-01 00:00:00

abstract：:Changes in mucin protein expression and in glycosylation are common features in pre-neoplastic lesions and cancer and are therefore used as cancer-associated markers. De novo expression of intestinal mucin MUC2 and cancer-associated sialyl-Tn antigen are frequently observed in intestinal metaplasia (IM) and gastric ca...

journal_title：Glycobiology

authors： Conze T,Carvalho AS,Landegren U,Almeida R,Reis CA,David L,Söderberg O

更新日期：2010-02-01 00:00:00

abstract：:Circulatory protein glycosylation is a biomarker of multiple disease and inflammatory states and has been applied in the clinic for liver dysfunction, heart disease and diabetes. With the notable exception of antibodies, the liver produces most of the circulatory glycoproteins, including the acute phase proteins relea...

journal_title：Glycobiology

authors： Oswald DM,Jones MB,Cobb BA

更新日期：2020-04-20 00:00:00

abstract：:The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...

journal_title：Glycobiology

authors： Hofinger ES,Spickenreither M,Oschmann J,Bernhardt G,Rudolph R,Buschauer A

更新日期：2007-04-01 00:00:00

abstract：:Endogenous lectins can control critical biological responses, including cell communication, signaling, angiogenesis and immunity by decoding glycan-containing information on a variety of cellular receptors and the extracellular matrix. Galectin-1 (Gal-1), a prototype member of the galectin family, displays only one ca...

journal_title：Glycobiology

authors： Romero JM,Trujillo M,Estrin DA,Rabinovich GA,Di Lella S

更新日期：2016-12-01 00:00:00

abstract：:The 165 amino acid form of vascular endothelial growth factor (VEGF165) is a heparin-binding growth factor with mitogenic activity for vascular endothelial cells. We examined activities of various heparin derivatives toward their interactions with VEGF165 using an enzyme-linked immunosorbent assay and elucidated the s...

journal_title：Glycobiology

authors： Ono K,Hattori H,Takeshita S,Kurita A,Ishihara M

更新日期：1999-07-01 00:00:00

abstract：:Streptococcus macedonicus is a Gram positive lactic acid bacterium that is part of the starter flora present in Greek sheep and goat cheeses. The S. macedonicus Sc136 strain produces a high-molecular-mass, highly texturizing exopolysaccharide composed of D-glucose, D-galactose, and N-acetyl-D-glucosamine in the molar ...

journal_title：Glycobiology

authors： Vincent SJ,Faber EJ,Neeser JR,Stingele F,Kamerling JP

更新日期：2001-02-01 00:00:00

abstract：:The hexosamine pathway (HP) is a biochemical hypothesis recently proposed explaining cellular alterations occurring during diabetic microvascular complications. Diabetic retinopathy is a common microvascular complication of diabetes, and it is known that cell proliferation is severely affected during the development o...

journal_title：Glycobiology

authors： Masson E,Wiernsperger N,Lagarde M,El Bawab S

更新日期：2005-06-01 00:00:00

abstract：:Escherichia coli K92 produces a capsular polysialic acid with alternating alpha2,8 alpha2,9 NeuNAc linkages. This polysaccharide is cross-reactive with the neuroinvasive pathogen Neisseria meningitidis Group C. The K92 polysialyltransferase (PST) catalyzes the synthesis of the polysialic acid with alternating linkages...

journal_title：Glycobiology

authors： Vionnet J,Vann WF

更新日期：2007-07-01 00:00:00

abstract：:Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene...

journal_title：Glycobiology

authors： Berends E,Lehle L,Henquet M,Hesselink T,Wösten HA,Lugones LG,Bosch D

更新日期：2013-02-01 00:00:00

abstract：:Many mannose-binding proteins inhibit divergent strains of human immunodeficiency virus type 1 (HIV-1) in in vitro models of viral infectivity, suggesting that targeting mannose residues in vaccine applications might offset the strain restriction typically observed in antibody responses to HIV vaccine preparations. Co...

journal_title：Glycobiology

authors： Pashov A,MacLeod S,Saha R,Perry M,VanCott TC,Kieber-Emmons T

更新日期：2005-10-01 00:00:00

abstract：:Intestinal maturation can be influenced by intrinsic factors (glucocorticoid hormones) and by extrinsic factors (resident microflora); their relative roles in ontogeny of mouse intestinal trehalase expression, a marker of general gut development, and of beta1,4-galactosyltransferase (beta GT), a marker of glycosyltran...

journal_title：Glycobiology

authors： Nanthakumar NN,Dai D,Meng D,Chaudry N,Newburg DS,Walker WA

更新日期：2005-03-01 00:00:00

abstract：:Human alpha-galactosidase A (alpha-Gal A) is the lysosomal glycohydrolase that cleaves the terminal alpha-galactosyl moieties of various glycoconjugates. Overexpression of the enzyme in Chinese hamster ovary (CHO) cells results in high intracellular enzyme accumulation and the selective secretion of active enzyme. Str...

journal_title：Glycobiology

authors： Matsuura F,Ohta M,Ioannou YA,Desnick RJ

更新日期：1998-04-01 00:00:00

abstract：:Tarin, the Colocasia esculenta lectin from the superfamily of α-d-mannose-specific plant bulb lectins, is a tetramer of 47 kDa composed of two heterodimers. Each heterodimer possesses homologous monomers of ~11.9 (A chain) and ~12.7 (B chain) kDa. The structures of apo and carbohydrate-bound tarin were solved to 1.7 Å...

journal_title：Glycobiology

authors： Pereira PR,Meagher JL,Winter HC,Goldstein IJ,Paschoalin VM,Silva JT,Stuckey JA

更新日期：2017-01-01 00:00:00

abstract：:Aspartic protease, widely used as a milk-coagulating agent in industrial cheese production, contains three potential N-glycosylation sites. In this study, we report the characterization of N-linked oligosaccharides on recombinant aspartic protease secreted from the methylotrophic yeast Pichia pastoris using a combinat...

journal_title：Glycobiology

authors： Montesino R,Nimtz M,Quintero O,García R,Falcón V,Cremata JA

更新日期：1999-10-01 00:00:00

abstract：:The human anticoagulant factor, Protein C, is a plasma glycoprotein that has reported anti-ischaemic and anti-inflammatory properties. To explore potential mechanisms for these reported activities, we examined the effect of Protein C on the process of cell adhesion to vascular endothelial cells, which plays a critical...

journal_title：Glycobiology

authors： Grinnell BW,Hermann RB,Yan SB

更新日期：1994-04-01 00:00:00

abstract：:Sialic acid has long been considered to be the sole receptor for influenza virus. The viral hemagglutinin (HA) is known to bind cell surface sialic acid, and sialic acids on viral glyco-proteins are cleaved by the viral neuraminidase (NA) to promote efficient release of progeny virus particles. However, NWS-Mvi, a mut...

journal_title：Glycobiology

authors： Stray SJ,Cummings RD,Air GM

更新日期：2000-07-01 00:00:00

abstract：:Heparan sulfate (HS) is a sulfated polysaccharide that plays a key role in morphogenesis, physiology and pathogenesis. The biosynthesis of HS takes place in the Golgi apparatus by a group of enzymes that polymerize, epimerize and sulfate the sugar chain. This biosynthetic process introduces varying degrees of sulfate ...

journal_title：Glycobiology

authors： Gesteira TF,Coulson-Thomas VJ

更新日期：2018-11-01 00:00:00

abstract：:Two kinds of molecules, one recognizing the sialo-/asialo-N-acetyllactosamine structures and the other recognizing the Lewis X structure in a divalent cation-independent manner, were detected on the head of boar sperm prepared from cauda epididymis by fluorescence-labeled or biotinylated dextran-based multivalent olig...

journal_title：Glycobiology

authors： Yoshitani N,Mori E,Takasaki S

更新日期：2001-04-01 00:00:00

abstract：:Polysialic acid (PSA) is thought to attenuate neural cell adhesion molecule (NCAM) adhesion, thereby facilitating neural cell migration and regeneration. Although the expression of PSA has been shown to correlate with the progression of certain tumors such as small cell lung carcinoma, there have been no studies to de...

journal_title：Glycobiology

authors： Suzuki M,Suzuki M,Nakayama J,Suzuki A,Angata K,Chen S,Sakai K,Hagihara K,Yamaguchi Y,Fukuda M

更新日期：2005-09-01 00:00:00

abstract：:There is increasing evidence that secretory fluids such as tears, saliva and milk play an important role in protecting the human body from infection via a washing mechanism involving glycan-mediated adhesion of potential pathogens to secretory glycoproteins. Interaction of sweat with bacteria is well established as th...

journal_title：Glycobiology

authors： Peterson RA,Gueniche A,Adam de Beaumais S,Breton L,Dalko-Csiba M,Packer NH

更新日期：2016-03-01 00:00:00

abstract：:Glycomics-an integrated approach to study structure-function relationships of complex carbohydrates (or glycans)-is an emerging field in this age of post-genomics. Realizing the importance of glycomics, many large scale research initiatives have been established to generate novel resources and technologies to advance ...

journal_title：Glycobiology

authors： Raman R,Venkataraman M,Ramakrishnan S,Lang W,Raguram S,Sasisekharan R

更新日期：2006-05-01 00:00:00

abstract：:The endoplasmic reticulum (ER) quality control system ensures that newly synthesized proteins in the early secretory pathway are in the correct conformation. Polypeptides that have failed to fold into native conformers are subsequently retrotranslocated and degraded by the cytosolic ubiquitin-proteasome system, a proc...

journal_title：Glycobiology

authors： Hosokawa N,Kamiya Y,Kato K

更新日期：2010-06-01 00:00:00

abstract：:Human skin fibroblasts were incubated in the presence of 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU). The culture medium was recovered and Xyl-MU derivatives which were initiated by the Xyl-MU acting as a primer were purified. As a result, a novel Xyl-MU derivative was isolated, in addition to previously reported Xy...

journal_title：Glycobiology

authors： Tazawa T,Takagaki K,Matsuya H,Nakamura T,Sasaki M,Endo M

更新日期：1998-09-01 00:00:00

abstract：:Trehalose synthase (TreS) catalyzes the reversible conversion of maltose into trehalose in mycobacteria as one of three biosynthetic pathways to this nonreducing disaccharide. Given the importance of trehalose to survival of mycobacteria, there has been considerable interest in understanding the enzymes involved in it...

journal_title：Glycobiology

authors： Caner S,Nguyen N,Aguda A,Zhang R,Pan YT,Withers SG,Brayer GD

更新日期：2013-09-01 00:00:00

abstract：:Mammalian alpha1,6-fucosyltransferase (FUT8) catalyses the transfer of a fucose residue from a donor substrate, guanosine 5'-diphosphate-beta-L-fucose to the reducing terminal N-acetylglucosamine (GlcNAc) of the core structure of an asparagine-linked oligosaccharide. Alpha1,6-fucosylation, also referred to as core fuc...

journal_title：Glycobiology

authors： Ihara H,Ikeda Y,Toma S,Wang X,Suzuki T,Gu J,Miyoshi E,Tsukihara T,Honke K,Matsumoto A,Nakagawa A,Taniguchi N

更新日期：2007-05-01 00:00:00

abstract：:Lutrophin (LH) consists of an array of isoforms with different charges and bioactivities. This study was undertaken to clarify specifically how oligosaccharides of alpha and beta subunits contribute to LH isoform charges. Porcine LH (pLH) was separated into four isoforms by isoelectric focusing (IEF), followed by subu...

journal_title：Glycobiology

authors： Ujihara M,Yamamoto K,Nomura K,Toyoshima S,Demura H,Nakamura Y,Ohmura K,Osawa T

更新日期：1992-06-01 00:00:00

abstract：:Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are primarily localized in the plasma membrane. Cholera toxin B subunit (Ctxb), a component of a heat-labile enterotoxin produced by Vibrio cholerae, has been frequently used as a probe to detect GM1 ganglio...

journal_title：Glycobiology

authors： Yanagisawa M,Ariga T,Yu RK

更新日期：2006-09-01 00:00:00

abstract：:Galectin 1 (GAL1) is a beta-galactoside-binding lectin involved in cell cycle progression. GAL1 overexpression is associated with neoplastic transformation and loss of differentiation. The gene encoding for human GAL1 resides on chromosome 22(q12; q13), and its expression is developmentally regulated. Although devoid ...

journal_title：Glycobiology

authors： Lutomski D,Fouillit M,Bourin P,Mellottée D,Denize N,Pontet M,Bladier D,Caron M,Joubert-Caron R

更新日期：1997-12-01 00:00:00