Delineation of the epitope recognized by an antibody specific for N-glycolylneuraminic acid-containing gangliosides.

abstract：:The deletion of the protein mannosyltransferase 1 gene (PMT1) of Saccharomyces cerevisiae results in viable cells. O-Mannosylation of proteins is reduced to about half of the value in comparison to wild-type cells. In order to distinguish between the the PMT1 gene product (= Pmt1p) and residual transferase activity, a...

journal_title：Glycobiology

authors： Gentzsch M,Strahl-Bolsinger S,Tanner W

更新日期：1995-02-01 00:00:00

abstract：:The hexosamine pathway (HP) is a biochemical hypothesis recently proposed explaining cellular alterations occurring during diabetic microvascular complications. Diabetic retinopathy is a common microvascular complication of diabetes, and it is known that cell proliferation is severely affected during the development o...

journal_title：Glycobiology

authors： Masson E,Wiernsperger N,Lagarde M,El Bawab S

更新日期：2005-06-01 00:00:00

abstract：:Protein O-fucosyltransferase 1 (Pofut1) and protein O-fucosyltransferase 2 (Pofut2) add O-linked fucose at distinct consensus sequences in properly folded epidermal growth factor (EGF)-like repeats and thrombospondin type-1 (TSR) repeats, respectively. Glycan chain elongation past O-fucose can occur to yield a tetrasa...

journal_title：Glycobiology

authors： Al-Shareffi E,Chaubard JL,Leonhard-Melief C,Wang SK,Wong CH,Haltiwanger RS

更新日期：2013-02-01 00:00:00

abstract：:Sialic acids can be modified by O-acetyl esters at the 7- and/or 9-position, altering recognition by antibodies, lectins and viruses. 9(7)-O-acetylation is mediated by a sialic acid-specific O-acetyltransferase, which has proven difficult to purify. Two groups have recently isolated cDNAs possibly encoding this enzyme...

journal_title：Glycobiology

authors： Shi WX,Chammas R,Varki A

更新日期：1998-02-01 00:00:00

abstract：:Angiogenesis is associated with growth, invasion, and metastasis of human solid tumors. Aberrant activation of endothelial cells and induction of microvascular permeability by a vascular endothelial growth factor (VEGF) receptor-2 (VEGFR-2) signaling pathway is observed in pathological angiogenesis including tumor, wo...

journal_title：Glycobiology

authors： Chung TW,Kim SJ,Choi HJ,Kim KJ,Kim MJ,Kim SH,Lee HJ,Ko JH,Lee YC,Suzuki A,Kim CH

更新日期：2009-03-01 00:00:00

abstract：:Epithelial cells in the lining of the intestines play critical roles in maintaining homeostasis while challenged by dynamic and sudden changes in luminal contents. Given the high density of glycosylation that encompasses their extracellular surface, environmental changes may lead to extensive reorganization of membran...

journal_title：Glycobiology

authors： Park D,Xu G,Barboza M,Shah IM,Wong M,Raybould H,Mills DA,Lebrilla CB

更新日期：2017-09-01 00:00:00

abstract：:Changes in mucin protein expression and in glycosylation are common features in pre-neoplastic lesions and cancer and are therefore used as cancer-associated markers. De novo expression of intestinal mucin MUC2 and cancer-associated sialyl-Tn antigen are frequently observed in intestinal metaplasia (IM) and gastric ca...

journal_title：Glycobiology

authors： Conze T,Carvalho AS,Landegren U,Almeida R,Reis CA,David L,Söderberg O

更新日期：2010-02-01 00:00:00

abstract：:Galectin 1 (GAL1) is a beta-galactoside-binding lectin involved in cell cycle progression. GAL1 overexpression is associated with neoplastic transformation and loss of differentiation. The gene encoding for human GAL1 resides on chromosome 22(q12; q13), and its expression is developmentally regulated. Although devoid ...

journal_title：Glycobiology

authors： Lutomski D,Fouillit M,Bourin P,Mellottée D,Denize N,Pontet M,Bladier D,Caron M,Joubert-Caron R

更新日期：1997-12-01 00:00:00

abstract：:Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was char...

journal_title：Glycobiology

authors： Pinto MR,Gorin PA,Wait R,Mulloy B,Barreto-Bergter E

更新日期：2005-10-01 00:00:00

abstract：:Endogenous lectins can control critical biological responses, including cell communication, signaling, angiogenesis and immunity by decoding glycan-containing information on a variety of cellular receptors and the extracellular matrix. Galectin-1 (Gal-1), a prototype member of the galectin family, displays only one ca...

journal_title：Glycobiology

authors： Romero JM,Trujillo M,Estrin DA,Rabinovich GA,Di Lella S

更新日期：2016-12-01 00:00:00

abstract：:Pig to human xenotransplantation is considered a possible solution to the prevailing chronic lack of human donor organs for allotransplantation. The Galalpha1,3Gal determinant is the major porcine xenogeneic epitope causing hyperacute rejection following human antibody binding and complement activation. In order to ch...

journal_title：Glycobiology

authors： Bäcker AE,Holgersson J,Samuelsson BE,Karlsson H

更新日期：1998-06-01 00:00:00

abstract：:Several tri- to hexasaccharide-alditols of the jelly coat surrounding the eggs of Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and 1H-NMR spectroscopy. As observed for six other amphibian species, these carbohydrate chains are highly species-specific. The main characteristics of the spe...

journal_title：Glycobiology

authors： Morelle W,Strecker G

更新日期：1997-09-01 00:00:00

abstract：:The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it i...

journal_title：Glycobiology

authors： Kanda Y,Yamada T,Mori K,Okazaki A,Inoue M,Kitajima-Miyama K,Kuni-Kamochi R,Nakano R,Yano K,Kakita S,Shitara K,Satoh M

更新日期：2007-01-01 00:00:00

abstract：:Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...

journal_title：Glycobiology

authors： Guarino C,DeLisa MP

更新日期：2012-05-01 00:00:00

abstract：:We have characterized a glycosphingolipid (GSL) receptor for Staphylococcus enterotoxin-B (SEB) in cultured human kidney proximal tubular (PT) cells. Solid-phase binding of [125I]SEB to the GSL receptor was concentration dependent and was not displaceable by two structurally related toxins, such as staphylococcal ente...

journal_title：Glycobiology

authors： Chatterjee S,Khullar M,Shi WY

更新日期：1995-05-01 00:00:00

abstract：:Streptococcus macedonicus is a Gram positive lactic acid bacterium that is part of the starter flora present in Greek sheep and goat cheeses. The S. macedonicus Sc136 strain produces a high-molecular-mass, highly texturizing exopolysaccharide composed of D-glucose, D-galactose, and N-acetyl-D-glucosamine in the molar ...

journal_title：Glycobiology

authors： Vincent SJ,Faber EJ,Neeser JR,Stingele F,Kamerling JP

更新日期：2001-02-01 00:00:00

abstract：:CAMPATH-1H (where CAMPATH is a trade mark of Wellcome group companies), a humanized IgG antibody used in the therapy of lymphoma, leukaemia and rheumatoid arthritis, has been expressed in Chinese hamster ovary, Y0 myeloma and NS0 myeloma cell lines. These engineered cell lines were grown under different culture condit...

journal_title：Glycobiology

authors： Lifely MR,Hale C,Boyce S,Keen MJ,Phillips J

更新日期：1995-12-01 00:00:00

abstract：:Exciting discoveries in many diverse fields of hyaluronan (HA) biology over the last 40 years have centered around the ability of HA to bind cell surface HA receptors (e.g., CD44, Layilin, LYVE-1, HARE/Stab2 and RHAMM) and sometimes also to activate intracellular signal transduction pathways, frequently involving ERK1...

journal_title：Glycobiology

authors： Weigel PH

更新日期：2017-09-01 00:00:00

abstract：:The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum...

journal_title：Glycobiology

authors： Inngjerdingen M,Inngjerdingen KT,Patel TR,Allen S,Chen X,Rolstad B,Morris GA,Harding SE,Michaelsen TE,Diallo D,Paulsen BS

更新日期：2008-12-01 00:00:00

abstract：:Diabetes mellitus was induced in one group of rats by a single injection of streptozotocin. The glycemia, the body weight, and the blood systolic pressure were measured every week, and the 24 h urine volume and urinary excretions of creatinine, albumin and glycosaminoglycans were measured every 2 weeks. At the end of ...

journal_title：Glycobiology

authors： Cadaval RA,Kohlman O,Michelacci YM

更新日期：2000-02-01 00:00:00

abstract：:We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the pu...

journal_title：Glycobiology

authors： Chan AL,Morris HR,Panico M,Etienne AT,Rogers ME,Gaffney P,Creighton-Kempsford L,Dell A

更新日期：1991-03-01 00:00:00

abstract：:N-Acetylglucosaminyltransferase-IV (GnT-IV) has two isoenzymes, GnT-IVa and GnT-IVb, which initiate the GlcNAcbeta1-4 branch synthesis on the Manalpha1-3 arm of the N-glycan core thereby increasing N-glycan branch complexity and conferring endogenous lectin binding epitopes. To elucidate the physiological significance...

journal_title：Glycobiology

authors： Takamatsu S,Antonopoulos A,Ohtsubo K,Ditto D,Chiba Y,Le DT,Morris HR,Haslam SM,Dell A,Marth JD,Taniguchi N

更新日期：2010-01-01 00:00:00

abstract：:Glycomics-an integrated approach to study structure-function relationships of complex carbohydrates (or glycans)-is an emerging field in this age of post-genomics. Realizing the importance of glycomics, many large scale research initiatives have been established to generate novel resources and technologies to advance ...

journal_title：Glycobiology

authors： Raman R,Venkataraman M,Ramakrishnan S,Lang W,Raguram S,Sasisekharan R

更新日期：2006-05-01 00:00:00

abstract：:Protozoan parasites of the genus Leishmania have a glycoconjugate surface coat (the glycocalyx) that acts as the interface between the parasite and its external environment. The principal components of the glycocalyx, the lipophosphoglycans and the glycoinositolphospholipids, have a variety of functions that facilitat...

journal_title：Glycobiology

authors： Smith DF,Rangarajan D

更新日期：1995-03-01 00:00:00

abstract：:Stage-specific embryonic antigen-1 (SSEA-1) is a well-known carbohydrate antigenic epitope of undifferentiated cells, including neural stem cells (NSCs). However, the exact nature of the carrier proteins has not been fully characterized. Using proteomics analyses, we herein report that a lysosomal protein, LAMP-1, is ...

journal_title：Glycobiology

authors： Yagi H,Yanagisawa M,Kato K,Yu RK

更新日期：2010-08-01 00:00:00

abstract：:The methylotrophic yeast, Pichia pastoris, is an important organism used for the production of therapeutic proteins. However, the presence of fungal-like glycans, such as those containing β-mannose (Man) linkages, can elicit an immune response or bind to Man receptors, thus reducing their efficacy. Recent studies have...

journal_title：Glycobiology

authors： Hopkins D,Gomathinayagam S,Rittenhour AM,Du M,Hoyt E,Karaveg K,Mitchell T,Nett JH,Sharkey NJ,Stadheim TA,Li H,Hamilton SR

更新日期：2011-12-01 00:00:00

abstract：:N-acetylglucosaminyltransferase V, encoded by the Mgat5 gene, plays an important regulatory role in the synthesis of complex N-glycans, including the Lewis antigens. The elevated expression of this enzyme is regulated during development and is highly associated with cellular transformation and malignancy. In addition,...

journal_title：Glycobiology

authors： Alverez K,Haswell C,St Clair M,Perng GS,Shorebah M,Pierce M,Fregien N

更新日期：2002-07-01 00:00:00

abstract：:Viscumin of mistletoe (Viscum album L.) has a concentration-dependent activity profile unique to plant AB-toxins. It starts with lectin-dependent mitogenicity and then covers toxicity and cell agglutination, associated with shifts in the monomer/dimer equilibrium. Each lectin subunit harbors two sections for ligand co...

journal_title：Glycobiology

authors： Jiménez M,André S,Siebert HC,Gabius HJ,Solís D

更新日期：2006-10-01 00:00:00

abstract：:A rat pheochromocytoma cell line (PC12), when transfected with beta1,4-N-acetylglucosaminyltransferase III (GnT-III), which catalyzes the formation of a bisecting GlcNAc structure in N-glycans, resulted in the suppression of neurite outgrowth induced by costimulation of epidermal growth factor (EGF) and integrins. The...

journal_title：Glycobiology

authors： Gu J,Zhao Y,Isaji T,Shibukawa Y,Ihara H,Takahashi M,Ikeda Y,Miyoshi E,Honke K,Taniguchi N

更新日期：2004-02-01 00:00:00

abstract：:Lectins are used as defense effector proteins against predators, parasites and pathogens by animal, plant and fungal innate defense systems. These proteins bind to specific glycoepitopes on the cell surfaces and thereby interfere with the proper cellular functions of the various antagonists. The exact cellular toxicit...

journal_title：Glycobiology

authors： Bleuler-Martinez S,Stutz K,Sieber R,Collot M,Mallet JM,Hengartner M,Schubert M,Varrot A,Künzler M

更新日期：2017-05-01 00:00:00