Ganglioside GM3 inhibits VEGF/VEGFR-2-mediated angiogenesis: direct interaction of GM3 with VEGFR-2.


:Angiogenesis is associated with growth, invasion, and metastasis of human solid tumors. Aberrant activation of endothelial cells and induction of microvascular permeability by a vascular endothelial growth factor (VEGF) receptor-2 (VEGFR-2) signaling pathway is observed in pathological angiogenesis including tumor, wound healing, arthritis, psoriasis, diabetic retinopathy, and others. Here, we show that GM3 regulated the activity of various downstream signaling pathways and biological events through the inhibition of VEGF-stimulated VEGFR-2 activation in vascular endothelial cells in vitro. Furthermore, GM3 strongly blocked VEGF-induced neovascularization in vivo, in models including the chick chorioallantoic membrane and Matrigel plug assay. Interestingly, GM3 suppressed VEGF-induced VEGFR-2 activation by blocking its dimerization and also blocked the binding of VEGF to VEGFR-2 through a GM3-specific interaction with the extracellular domain of VEGFR-2, but not with VEGF. Primary tumor growth in mice was inhibited by subcutaneous injection of GM3. Immunohistochemical analyses showed GM3 inhibition of angiogenesis and tumor cell proliferation. GM3 also resulted in the suppression of VEGF-stimulated microvessel permeability in mouse skin capillaries. These results suggest that GM3 inhibits VEGFR-2-mediated changes in vascular endothelial cell function and angiogenesis, and might be of value in anti-angiogenic therapy.






Chung TW,Kim SJ,Choi HJ,Kim KJ,Kim MJ,Kim SH,Lee HJ,Ko JH,Lee YC,Suzuki A,Kim CH




Has Abstract


2009-03-01 00:00:00














  • β4GalT6 is involved in the synthesis of lactosylceramide with less intensity than β4GalT5.

    abstract::Glycosphingolipids are expressed on the cell membrane and act as important factors in various events that occur across the plasma membrane. Lactosylceramide (LacCer) is synthesized from glucosylceramide and is a common precursor of various glycosphingolipids existing in whole body. Based on the enzyme purification, β1...


    pub_type: 杂志文章


    authors: Tokuda N,Numata S,Li X,Nomura T,Takizawa M,Kondo Y,Yamashita Y,Hashimoto N,Kiyono T,Urano T,Furukawa K,Furukawa K

    更新日期:2013-10-01 00:00:00

  • The effect of oral treatment with 6-O-butanoyl castanospermine (MDL 28,574) in the murine zosteriform model of HSV-1 infection.

    abstract::Oral treatment of mice, cutaneously infected with herpes simplex virus type 1 (HSV-1) (strain SC16), with the alpha-glucosidase 1 inhibitor 6-O-butanoyl castanospermine (MDL 28,574) produced a significant delay in lesion development and reduced the amount of virus recovered from the brain. Virus load in the brains of ...


    pub_type: 杂志文章


    authors: Bridges CG,Ahmed SP,Kang MS,Nash RJ,Porter EA,Tyms AS

    更新日期:1995-03-01 00:00:00

  • Revisiting the human polypeptide GalNAc-T1 and T13 paralogs.

    abstract::Polypeptide GalNAc-transferases (GalNAc-Ts) constitute a family of 20 human glycosyltransferases (comprising 9 subfamilies), which initiate mucin-type O-glycosylation. The O-glycoproteome is thought to be differentially regulated via the different substrate specificities and expression patterns of each GalNAc-T isofor...


    pub_type: 杂志文章


    authors: Festari MF,Trajtenberg F,Berois N,Pantano S,Revoredo L,Kong Y,Solari-Saquieres P,Narimatsu Y,Freire T,Bay S,Robello C,Bénard J,Gerken TA,Clausen H,Osinaga E

    更新日期:2017-01-01 00:00:00

  • Recent advancements in understanding mammalian O-mannosylation.

    abstract::The post-translational glycosylation of select proteins by O-linked mannose (O-mannose or O-man) is a conserved modification from yeast to humans and has been shown to be necessary for proper development and growth. The most well studied O-mannosylated mammalian protein is α-dystroglycan (α-DG). Hypoglycosylation of α...


    pub_type: 杂志文章,评审


    authors: Sheikh MO,Halmo SM,Wells L

    更新日期:2017-09-01 00:00:00

  • Variant heparan sulfates synthesized in developing mouse brain differentially regulate FGF signaling.

    abstract::Heparan sulfates (HSs) exert critical regulatory actions on many proteins, including growth factors, and are essential for normal development. Variations in their specific sulfation patterns are known to regulate binding and signaling of fibroblast growth factors (FGFs) via tyrosine kinase receptors (FGFRs). We previo...


    pub_type: 杂志文章


    authors: Ford-Perriss M,Guimond SE,Greferath U,Kita M,Grobe K,Habuchi H,Kimata K,Esko JD,Murphy M,Turnbull JE

    更新日期:2002-11-01 00:00:00

  • Cell surface components of Leishmania: identification of a novel parasite lectin?

    abstract::Protozoan parasites of the genus Leishmania have a glycoconjugate surface coat (the glycocalyx) that acts as the interface between the parasite and its external environment. The principal components of the glycocalyx, the lipophosphoglycans and the glycoinositolphospholipids, have a variety of functions that facilitat...


    pub_type: 杂志文章,评审


    authors: Smith DF,Rangarajan D

    更新日期:1995-03-01 00:00:00

  • Structural characterization of the N-linked oligosaccharides in bile salt-stimulated lipase originated from human breast milk.

    abstract::The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The N- glycan structures were conclusively determined in terms of complexity...


    pub_type: 杂志文章


    authors: Mechref Y,Chen P,Novotny MV

    更新日期:1999-03-01 00:00:00

  • Planning, evaluating and vetting receptor signaling studies to assess hyaluronan size-dependence and specificity.

    abstract::Exciting discoveries in many diverse fields of hyaluronan (HA) biology over the last 40 years have centered around the ability of HA to bind cell surface HA receptors (e.g., CD44, Layilin, LYVE-1, HARE/Stab2 and RHAMM) and sometimes also to activate intracellular signal transduction pathways, frequently involving ERK1...


    pub_type: 杂志文章,评审


    authors: Weigel PH

    更新日期:2017-09-01 00:00:00

  • Purification and cDNA cloning of Xenopus liver galectins and their expression.

    abstract::We have characterized galectin family proteins in adult tissues of Xenopus laevis and purified 14-kDa and 36-kDa proteins from the liver. The liver galectins showed comparable hemagglutination activities to those of mammalian galectins. Furthermore, we isolated five galectin cDNAs from a Xenopus liver library. These c...


    pub_type: 杂志文章


    authors: Shoji H,Nishi N,Hirashima M,Nakamura T

    更新日期:2002-03-01 00:00:00

  • Modulation of the expression of the rabbit galectin-3 gene by p53 and c-Ha-ras proteins and PMA.

    abstract::Galectin-3 is a galactose-binding lectin that has been found in several mammalian tissues. Galectin-3 gene is expressed in a wide range of normal and tumoral cells. In the case of myeloid cells, its expression correlates with the differentiation of monocytes to macrophages. In the case of cancer cell lines, its expres...


    pub_type: 杂志文章


    authors: Gaudin JC,Arar C,Monsigny M,Legrand A

    更新日期:1997-12-01 00:00:00

  • Antiganglioside antibodies and their pathophysiological effects on Guillain-Barré syndrome and related disorders--a review.

    abstract::Guillain-Barré syndrome (GBS) is an acute immune-mediated polyradiculoneuropathy which can cause acute quadriplegia. Infection with micro-organisms, including Campylobacter jejuni (C. jejuni), Haemophilus influenzae, and Cytomegalovirus (CMV), is recognized as a main triggering event for the disease. Lipooligosacchari...


    pub_type: 杂志文章,评审


    authors: Kaida K,Ariga T,Yu RK

    更新日期:2009-07-01 00:00:00

  • What is special about 200 kDa hyaluronan that activates hyaluronan receptor signaling?

    abstract::The polydispersity of hyaluronan (HA) presents challenges for analyzing its solution properties, such as the relationship between mass and particle size. The broad mass range of natural HA (≤50-fold) makes molecular characterization difficult and ambiguous compared to molecules with known molecular weights (e.g., prot...


    pub_type: 杂志文章


    authors: Weigel PH,Baggenstoss BA

    更新日期:2017-09-01 00:00:00

  • Core oligosaccharide structure from the highly phytopathogenic Agrobacterium tumefaciens TT111 and conformational analysis of the putative rhamnan epitope.

    abstract::The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...


    pub_type: 杂志文章


    authors: De Castro C,Carannante A,Lanzetta R,Liparoti V,Molinaro A,Parrilli M

    更新日期:2006-12-01 00:00:00

  • Lactosylceramide promotes hypertrophy through ROS generation and activation of ERK1/2 in cardiomyocytes.

    abstract::Hypertrophy is central to several heart diseases; however, not much is known about the role of glycosphingolipids (GSLs) in this phenotype. Since GSLs have been accorded several physiological functions, we sought to determine whether these compounds affect cardiac hypertrophy. By using a rat cardiomyoblast cell line, ...


    pub_type: 杂志文章


    authors: Mishra S,Chatterjee S

    更新日期:2014-06-01 00:00:00

  • Utilization of major fucosylated and sialylated human milk oligosaccharides by isolated human gut microbes.

    abstract::Human milk oligosaccharides (HMOS) are not digested in the proximal intestine. In distal intestine, HMOS collectively modify the microbiota, but the response of individual bacteria to individual components of the HMOS is not well defined. Here, each of 25 major isolates of the human intestinal microbiota was fed indiv...


    pub_type: 杂志文章


    authors: Yu ZT,Chen C,Newburg DS

    更新日期:2013-11-01 00:00:00

  • A novel sialylated N-acetylgalactosamine-containing oligosaccharide is the major complex-type structure present in Bowes melanoma tissue plasminogen activator.

    abstract::We have employed fast atom bombardment mass spectrometry (FAB-MS) to screen the N-linked oligosaccharides of Bowes melanoma tissue plasminogen activator (mt-PA), and recombinant t-PAs produced by Chinese hamster ovary cells (rt-PA) and by a gene-enriched melanoma cell line (rmt-PA). These studies have confirmed the pu...


    pub_type: 杂志文章


    authors: Chan AL,Morris HR,Panico M,Etienne AT,Rogers ME,Gaffney P,Creighton-Kempsford L,Dell A

    更新日期:1991-03-01 00:00:00

  • Specific activation of ERK pathways by chitin oligosaccharides in embryonic zebrafish cell lines.

    abstract::Chitin oligosaccharides (COs) play a role in plant development and are presumed to affect body plan formation during vertebrate embryogenesis. The mechanisms of COs recognition and cellular processes underlying embryonic development are still not understood. We analyze the possible link with the mitogen-activated prot...


    pub_type: 杂志文章


    authors: Snaar-Jagalska BE,Krens SF,Robina I,Wang LX,Spaink HP

    更新日期:2003-10-01 00:00:00

  • Remodeling of sugar chain structures of human interferon-gamma.

    abstract::Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetyl...


    pub_type: 杂志文章


    authors: Fukuta K,Abe R,Yokomatsu T,Kono N,Asanagi M,Omae F,Minowa MT,Takeuchi M,Makino T

    更新日期:2000-04-01 00:00:00

  • Epidermal growth factor receptor glycosylation is required for ganglioside GM3 binding and GM3-mediated suppression [correction of suppresion] of activation.

    abstract::Gangliosides are able to bind to the epidermal growth factor receptor and inhibit its activation, but the mechanism of this inhibition is unknown. To address the role of receptor carbohydrates in facilitating interaction with gangliosides, we examined the ability of GM3 to bind the deglycosylated receptor and inhibit ...


    pub_type: 杂志文章


    authors: Wang XQ,Sun P,O'Gorman M,Tai T,Paller AS

    更新日期:2001-07-01 00:00:00

  • Characterization of the meningococcal serogroup X capsule N-acetylglucosamine-1-phosphotransferase.

    abstract::Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of th...


    pub_type: 杂志文章


    authors: Muindi KM,McCarthy PC,Wang T,Vionnet J,Battistel M,Jankowska E,Vann WF

    更新日期:2014-02-01 00:00:00

  • The initial noncovalent binding of glucose to human hemoglobin in nonenzymatic glycation.

    abstract::Mechanisms for nonenzymatic protein glycation have been extensively studied albeit with an emphasis at the later stages that gives rise to advanced glycation end products. No detailed investigation of the initial, noncovalent binding of d-glucose to human hemoglobin A (HbA) exists in the literature. Although anionic m...


    pub_type: 杂志文章


    authors: Clark SL,Santin AE,Bryant PA,Holman R,Rodnick KJ

    更新日期:2013-11-01 00:00:00

  • Rapid and sensitive GC/MS characterization of glycolipid released Galalpha1,3Gal-terminated oligosaccharides from small organ specimens of a single pig.

    abstract::Pig to human xenotransplantation is considered a possible solution to the prevailing chronic lack of human donor organs for allotransplantation. The Galalpha1,3Gal determinant is the major porcine xenogeneic epitope causing hyperacute rejection following human antibody binding and complement activation. In order to ch...


    pub_type: 杂志文章


    authors: Bäcker AE,Holgersson J,Samuelsson BE,Karlsson H

    更新日期:1998-06-01 00:00:00

  • Selective expression of different fucosylated epitopes on two distinct sets of Schistosoma mansoni cercarial O-glycans: identification of a novel core type and Lewis X structure.

    abstract::The glycobiology of Schistosoma mansoni is dominated by developmentally regulated expression of various fucosylated structures, most notably the Lewis X epitope and a multifucosylated sequence, Fuc alpha1-->2Fuc alpha1-->, in its various forms. For the infective cercarial stage, Lewis X has been structurally identifie...


    pub_type: 杂志文章


    authors: Huang HH,Tsai PL,Khoo KH

    更新日期:2001-05-01 00:00:00

  • Advanced mass spectrometry and chemical analyses reveal the presence of terminal disialyl motif on mouse B-cell glycoproteins.

    abstract::The occurrence of a terminal disialyl motif on mammalian O-glycans is increasingly being identified through recent mass spectrometry (MS)-based glycomic profiling. In most cases, it is carried on simple core 1 structures in which both the galactose and N-acetyl galactosamine can be disialylated. In contrast, a disialy...


    pub_type: 杂志文章


    authors: Wang SH,Tsai CM,Lin KI,Khoo KH

    更新日期:2013-06-01 00:00:00

  • Specificity of xenoreactive anti-Gal alpha 1-3Gal IgM for alpha-galactosyl ligands.

    abstract::The transplantation of organs from lower animals such as pigs into humans is prevented by a severe rejection reaction initiated by complement fixing xenoreactive natural antibodies. Most anti-pig xenoreactive natural antibodies in humans are thought to recognize Gal alpha 1-3Gal beta 1-4GlcNAc and are also thought to ...


    pub_type: 杂志文章


    authors: Parker W,Lateef J,Everett ML,Platt JL

    更新日期:1996-07-01 00:00:00

  • Carbohydrate recognition factors of a Talpha (Galbeta1-->3GalNAcalpha1-->Ser/Thr) and Tn (GalNAcalpha1-->Ser/Thr) specific lectin isolated from the seeds of Artocarpus lakoocha.

    abstract::Artocarpus lakoocha agglutinin (ALA), isolated from the seeds of A. lakoocha fruit, is a galactose-binding lectin and a potent mitogen of T and B cells. Knowledge obtained from previous studies on the affinity of ALA was limited to molecular and submolecular levels of Galbeta1-->3GalNAc (T) and its derivatives. In the...


    pub_type: 杂志文章


    authors: Singh T,Chatterjee U,Wu JH,Chatterjee BP,Wu AM

    更新日期:2005-01-01 00:00:00

  • Sialic acid content of tissue-specific gp96 and its potential role in modulating gp96-macrophage interactions.

    abstract::Cancer-derived heat shock protein gp96 induces a tumor-specific protective immune response primarily mediated by cytotoxic T lymphocytes (CTL) directed toward cancer-associated peptides associated with gp96. Both innate and adaptive immune responses have been demonstrated using a cell culture-based signaling mechanism...


    pub_type: 杂志文章


    authors: Suriano R,Ghosh SK,Chaudhuri D,Mittelman A,Banerjee A,Tiwari RK

    更新日期:2009-12-01 00:00:00

  • The dendritic cell-specific C-type lectin DC-SIGN is a receptor for Schistosoma mansoni egg antigens and recognizes the glycan antigen Lewis x.

    abstract::Schistosoma mansoni soluble egg antigens (SEAs) are crucially involved in modulating the host immune response to infection by S. mansoni. We report that human dendritic cells bind SEAs through the C-type lectin dendritic cell-specific ICAM-3-grabbing nonintegrin (DC-SIGN). Monoclonal antibodies against the carbohydrat...


    pub_type: 杂志文章


    authors: van Die I,van Vliet SJ,Nyame AK,Cummings RD,Bank CM,Appelmelk B,Geijtenbeek TB,van Kooyk Y

    更新日期:2003-06-01 00:00:00

  • The minimum information required for a glycomics experiment (MIRAGE) project: LC guidelines.

    abstract::The Minimum Information Required for a Glycomics Experiment (MIRAGE) is an initiative created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to design guidelines that improve the reporting and reproducibility of glycoanalytical methods. Previously, the MIRAGE Commission has published gui...


    pub_type: 杂志文章


    authors: Campbell MP,Abrahams JL,Rapp E,Struwe WB,Costello CE,Novotny M,Ranzinger R,York WS,Kolarich D,Rudd PM,Kettner C

    更新日期:2019-05-01 00:00:00

  • Forssman disaccharide is the specific ligand of a galectin from the sponge Geodia cydonium but does not mediate its binding to nuclear protein np56.

    abstract::The galectin from Geodia cydonium (GCA) had previously been shown to be involved in regulatory mechanisms of cell sorting and adhesion during reaggregation of allogeneic sponge cells. In this contribution the binding specificity of GCA was established to be GalNAc alpha 1-3GalNAc beta as structural component of Forssm...


    pub_type: 杂志文章


    authors: Hanisch FG,Baldus SE,Kümmel TA

    更新日期:1996-04-01 00:00:00