Abstract:
:Several tri- to hexasaccharide-alditols of the jelly coat surrounding the eggs of Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and 1H-NMR spectroscopy. As observed for six other amphibian species, these carbohydrate chains are highly species-specific. The main characteristics of the species Bufo bufo consists in the presence of the new carbohydrate sequence Gal(alpha1-3)GalNAc(alpha1-3)[Fuc(alpha1-2)]Gal, in which a blood group A determinant is substituted with an external alpha1-3 linked galactose unit. Since the role of carbohydrates appears more and more apparent during the fertilization processes, the species-specificity of these carbohydrate moieties should be relevant to the species-specific gamete recognition which characterizes most amphibians.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Morelle W,Strecker Gdoi
10.1093/glycob/7.6.777subject
Has Abstractpub_date
1997-09-01 00:00:00pages
777-90issue
6eissn
0959-6658issn
1460-2423journal_volume
7pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::The binding properties of Pseudomonas aeruginosa agglutinin-I (PA-IL) with glycoproteins (gps) and polysaccharides were studied by both the biotin/avidin-mediated microtiter plate lectin-binding assay and the inhibition of agglutinin-glycan interaction with sugar ligands. Among 36 glycans tested for binding, PA-IL rea...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.1.7
更新日期:1998-01-01 00:00:00
abstract::Pig to human xenotransplantation is considered a possible solution to the prevailing chronic lack of human donor organs for allotransplantation. The Galalpha1,3Gal determinant is the major porcine xenogeneic epitope causing hyperacute rejection following human antibody binding and complement activation. In order to ch...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.6.533
更新日期:1998-06-01 00:00:00
abstract::To date, there is no complete structural characterization of human milk glycosaminoglycans (GAGs) available nor do any data exist on their composition in bovine milk. Total GAGs were determined on extracts from human and bovine milk. Samples were subjected to digestion with specific enzymes, treated with nitrous acid,...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq164
更新日期:2011-03-01 00:00:00
abstract::Apolipoprotein C-III (apoCIII) is a small glycoprotein with a single mucin-type core-1 oligosaccharide and is analyzed by isoelectric focusing (IEF) for the diagnosis of genetic defects in O-glycan biosynthesis such as congenital disorders of glycosylation. In the present study, mass spectrometry of apoCIII, after a s...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws086
更新日期:2012-08-01 00:00:00
abstract::We have characterized a glycosphingolipid (GSL) receptor for Staphylococcus enterotoxin-B (SEB) in cultured human kidney proximal tubular (PT) cells. Solid-phase binding of [125I]SEB to the GSL receptor was concentration dependent and was not displaceable by two structurally related toxins, such as staphylococcal ente...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.3.327
更新日期:1995-05-01 00:00:00
abstract::The trehalose-P synthase was purified to near homogeneity from the cytoplasmic fraction of Mycobacterium smegmatis. At the final stage of purification, the enzyme preparation showed one major band of 59 kDa on SDS gels. The 59 kDa band became labeled with N3-UDP[32P]-glucose, and this labeling was inhibited in a conce...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/6.4.453
更新日期:1996-06-01 00:00:00
abstract::The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transf...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.7.683
更新日期:1995-10-01 00:00:00
abstract::Polysialic acid (PSA) is thought to attenuate neural cell adhesion molecule (NCAM) adhesion, thereby facilitating neural cell migration and regeneration. Although the expression of PSA has been shown to correlate with the progression of certain tumors such as small cell lung carcinoma, there have been no studies to de...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi071
更新日期:2005-09-01 00:00:00
abstract::The presence of nonmammalian core alpha(1,3)-fucose and core xylose glyco-epitopes on glycans N-linked to therapeutic glycoproteins produced in plants has raised the question of their immunogenicity in human therapy. We address this question by studying the distribution of these N-glycans in pea, rice, and maize (whic...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg024
更新日期:2003-06-01 00:00:00
abstract::Intelectin is an extracellular animal lectin found in chordata. Although human and mouse intelectin-1 recognize galactofuranosyl residues included in cell walls of various microorganisms, the physiological function of mammalian intelectin had been unclear. In this study, we found that human intelectin-1 was a serum pr...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp013
更新日期:2009-05-01 00:00:00
abstract::Based on chemical analysis, we have previously concluded that the biologically important lipoarabinomannan (LAM) and lipomannan (LM) from Mycobacterium are multiglycosylated forms of the phosphatidylinositol mannosides (PIMs), the characteristic cell envelope mannophosphoinositides of mycobacteria. Using definitive an...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.1.117
更新日期:1995-02-01 00:00:00
abstract::Heparan sulfate is a polysaccharide belonging to the glycaminoglycan family. It interacts with numerous proteins of the extracellular matrix, in particular cellular growth factors. The number of experimental protein-heparin sulfate complexes obtained by crystallography or nuclear magnetic resonance is limited. Alterna...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr052
更新日期:2011-09-01 00:00:00
abstract::Mac-2 is a member of the S-(soluble) lectin family. Its identification and isolation from a wide variety of cell types and tissues suggest a diversity of roles in various biological systems. The key points to be made are that Mac-2, and the S-lectins in general, by virtue of their recognition of a variety of carbohydr...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/4.1.5
更新日期:1994-02-01 00:00:00
abstract::Galbeta1-4GlcNAc is thought to be a common disaccharide unit preferentially recognized by vertebrate galectins. Eight-amino-acid residues conserved in proteins belonging to the galectin family have been suggested to be responsible for recognition. Meanwhile, we isolated and analyzed endogenous N-glycans of Caenorhabdi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp125
更新日期:2009-12-01 00:00:00
abstract::Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion. The activity was solubilized by t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/1.6.605
更新日期:1991-12-01 00:00:00
abstract::P3 is a mouse monoclonal antibody (mAb) that binds to several NeuGc-containing gangliosides. It also reacts with antigens expressed in human breast tumors (Vázquez et al. (1995) Hybridoma , 14, 551-556). In this work, the binding specificity of P3 has been characterized in more detail using a panel of glycolipids that...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.7.695
更新日期:1998-07-01 00:00:00
abstract::Herein the biodistributions and in vivo kinetics of chemically prepared neoglycoproteins are reviewed. Chemical methods can be used to conjugate various mono- and oligosaccharides onto a protein surface. The kinetics and organ-specific accumulation profiles of these glycoconjugates, which are introduced through intrav...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cww038
更新日期:2016-08-01 00:00:00
abstract::A large family of UDP-GalNAc:polypeptide GalNAc transferases (ppGalNAc-Ts) initiates and defines sites of mucin-type Ser/Thr-O-GalNAc glycosylation. Family members have been classified into peptide- and glycopeptide-preferring subfamilies, although both families possess variable activities against glycopeptide substra...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwv108
更新日期:2016-04-01 00:00:00
abstract::The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl033
更新日期:2006-12-01 00:00:00
abstract::Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.1.37
更新日期:2001-01-01 00:00:00
abstract::UDP-N-acetylglucosamine (UDP-GlcNAc) is a glucose metabolite with pivotal functions as a key substrate for the synthesis of glycoconjugates like hyaluronan, and as a metabolic sensor that controls cell functions through O-GlcNAc modification of intracellular proteins. However, little is known about the regulation of h...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww019
更新日期:2016-07-01 00:00:00
abstract::Galectin-1, the prototype of a family of beta-galactoside-binding proteins, has been implicated in a wide variety of biological processes. Data presented herein show that galectin-1 stimulates monocyte migration in a dose-dependent manner but is not chemotactic for macrophages. Galectin-1-induced monocyte chemotaxis i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp077
更新日期:2009-12-01 00:00:00
abstract::Endolysins are bacteriophage enzymes that lyse their bacterial host for phage progeny release. They commonly contain an N-terminal catalytic domain that hydrolyzes bacterial peptidoglycan (PG) and a C-terminal cell wall-binding domain (CBD) that confers enzyme localization to the PG substrate. Two endolysins, phage ly...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt019
更新日期:2013-07-01 00:00:00
abstract::Heparan sulfate (HS) is a sulfated polysaccharide that plays a key role in morphogenesis, physiology and pathogenesis. The biosynthesis of HS takes place in the Golgi apparatus by a group of enzymes that polymerize, epimerize and sulfate the sugar chain. This biosynthetic process introduces varying degrees of sulfate ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy055
更新日期:2018-11-01 00:00:00
abstract::Ganglioside GM3 (Siaα2-3Galβ1-4Glcβ1-1Cer) has been known to participate in insulin signaling by regulating the association of the insulin receptor in caveolae microdomains (lipid rafts), which is essential for the execution of the complete insulin metabolic signaling in adipocytes. Macrophage-secreted factors includi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu112
更新日期:2015-03-01 00:00:00
abstract::Endogenous lectins can control critical biological responses, including cell communication, signaling, angiogenesis and immunity by decoding glycan-containing information on a variety of cellular receptors and the extracellular matrix. Galectin-1 (Gal-1), a prototype member of the galectin family, displays only one ca...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww052
更新日期:2016-12-01 00:00:00
abstract::Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of th...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt091
更新日期:2014-02-01 00:00:00
abstract::Human milk oligosaccharides (HMOS) are not digested in the proximal intestine. In distal intestine, HMOS collectively modify the microbiota, but the response of individual bacteria to individual components of the HMOS is not well defined. Here, each of 25 major isolates of the human intestinal microbiota was fed indiv...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt065
更新日期:2013-11-01 00:00:00
abstract::Glycosyltransferases, usually residing within the intracellular secretory apparatus, also circulate in the blood. Many of these blood-borne glycosyltransferases are associated with pathological states, including malignancies and inflammatory conditions. Despite the potential for dynamic modifications of glycans on dis...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww108
更新日期:2017-01-01 00:00:00
abstract::Sialic acids can be modified by O-acetyl esters at the 7- and/or 9-position, altering recognition by antibodies, lectins and viruses. 9(7)-O-acetylation is mediated by a sialic acid-specific O-acetyltransferase, which has proven difficult to purify. Two groups have recently isolated cDNAs possibly encoding this enzyme...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.2.199
更新日期:1998-02-01 00:00:00