Abstract:
:Neisseria meningitidis serogroups A, B, C, Y, W135 and X are responsible for most cases of meningococcal meningitis. Neisseria meningitidis serogroup X has recently emerged as a contributor to outbreaks of disease in Africa, but there is currently no vaccine against serogroup X. Understanding of the biosynthesis of the serogroup X capsular polysaccharide would provide useful tools for vaccine production. The serogroup X polysaccharide is a homopolymer of (α1→4)-linked N-acetylglucosamine (GlcNAc)-1-phosphate. It has been shown that the gene cluster xcbABC encodes synthesis of this polysaccharide. The xcbA gene product has significant homology with sacB, which is responsible for synthesis of the Neisseria serogroup A capsular polysaccharide, an (α1→6)-N-acetylmannosamine-1-phosphate homopolymer. The xcbA protein also shares homology with the catalytic domain of human N-acetylglucosamine-1-phosphoryltransferase, a key enzyme in the mannose-6-phosphate receptor pathway. In this study, we show that xcbA in the appropriate background is sufficient for the synthesis of N. meningitidis serogroup X polysaccharide. By ELISA we detected polysaccharide in fractions of Escherichia coli expressing the xcbA gene. We isolated polysaccharide from an E. coli strain expressing XcbA and demonstrated that this polysaccharide has a (13)C-NMR spectrum identical to that of polysaccharide isolated from N. meningitidis Group X. We also demonstrate that the purified XcbA protein is an N-acetylglucosamine-1-phosphotransferase that transfers N-acetylglucosamine-1-phosphate from UDP-GlcNAc to the 4-hydroxyl of an N-acetylglucosamine-1-phosphate oligosaccharide. Oligosaccharides fluorescently labeled at the aglycon are extended by XcbA only after the 4-phosphate occupying the non-reducing GlcNAc has been removed. The minimum size of fluorescent acceptors is a trisaccharide.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Muindi KM,McCarthy PC,Wang T,Vionnet J,Battistel M,Jankowska E,Vann WFdoi
10.1093/glycob/cwt091subject
Has Abstractpub_date
2014-02-01 00:00:00pages
139-49issue
2eissn
0959-6658issn
1460-2423pii
cwt091journal_volume
24pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::Endolysins are bacteriophage enzymes that lyse their bacterial host for phage progeny release. They commonly contain an N-terminal catalytic domain that hydrolyzes bacterial peptidoglycan (PG) and a C-terminal cell wall-binding domain (CBD) that confers enzyme localization to the PG substrate. Two endolysins, phage ly...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt019
更新日期:2013-07-01 00:00:00
abstract::The substrate specificity of an alpha2,3-sialyltransferase (v-ST3Gal I) obtained from myxoma virus infected RK13 cells has been determined. Like mammalian sialyltransferase enzymes, the viral enzyme contains the characteristic L- and S-sialyl motif sequences in its catalytic domain. Analysis of the deduced amino acid ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.3.313
更新日期:2000-03-01 00:00:00
abstract::The syp locus includes four genes encoding putative regulators, six genes encoding glycosyltransferases, two encoding export proteins, and six other genes encoding unidentified functional proteins associated with biofilm formation and symbiotic colonization. However, the individual functions of the respective genes re...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu001
更新日期:2014-04-01 00:00:00
abstract::Sialic acids cap the glycans of cell surface glycoproteins and glycolipids. They are involved in a multitude of biological processes and aberrant sialic acid expression is associated with several pathologies. Sialic acids modulate the characteristics and functions of glycoproteins and regulate cell-cell as well as cel...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz026
更新日期:2019-06-01 00:00:00
abstract::It is demonstrated with glycopeptides of the polymorphic epithelial mucin (MUC1) that post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) is a fast, highly sensitive, and reproducible method for the localization of O-glycosylation sites by reflectron time-of-flight (TOF) mass spectrometry. We hav...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.7.881
更新日期:1997-10-01 00:00:00
abstract::Several tri- to hexasaccharide-alditols of the jelly coat surrounding the eggs of Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and 1H-NMR spectroscopy. As observed for six other amphibian species, these carbohydrate chains are highly species-specific. The main characteristics of the spe...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.6.777
更新日期:1997-09-01 00:00:00
abstract::The human intestine houses a dense microbial ecosystem in which the struggle for nutrients creates a continual and dynamic selective force. Host-produced mucus glycans provide a ubiquitous source of carbon and energy for microbial species. Not surprisingly, many gut resident bacteria have become highly adapted to effi...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwt040
更新日期:2013-09-01 00:00:00
abstract::The presence of nonmammalian core alpha(1,3)-fucose and core xylose glyco-epitopes on glycans N-linked to therapeutic glycoproteins produced in plants has raised the question of their immunogenicity in human therapy. We address this question by studying the distribution of these N-glycans in pea, rice, and maize (whic...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg024
更新日期:2003-06-01 00:00:00
abstract::Circulating leukocytes that express selectin ligands such as the carbohydrate epitope sialyl Lewis X (sLeX) may interact with endothelial selectins, resulting in transmigration of the leukocyte across the endothelial wall to adjacent tissue. Due to the potential of selectin-ligand interactions as targets in viral path...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws160
更新日期:2013-03-01 00:00:00
abstract::Spectrofluorimetry experiments using synthetic trisaccharides indicate that in compounds that display affinity for antithrombin III (AT-III), a unique trisaccharide sequence plays the key role in the early recognition, and the first step of AT-III activation. Added to previous observations, these new results suggest t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.3.323-e
更新日期:1997-04-01 00:00:00
abstract::Lutrophin (LH) consists of an array of isoforms with different charges and bioactivities. This study was undertaken to clarify specifically how oligosaccharides of alpha and beta subunits contribute to LH isoform charges. Porcine LH (pLH) was separated into four isoforms by isoelectric focusing (IEF), followed by subu...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/2.3.225
更新日期:1992-06-01 00:00:00
abstract::Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr151
更新日期:2012-05-01 00:00:00
abstract::Human noroviruses (HuNoV), members of the family Caliciviridae, are the major cause of acute viral gastroenteritis worldwide. Successful infection is linked to the ability of the protruding (P) domain of the viral capsid to bind histo-blood group antigens (HBGA). Binding to gangliosides plays a major role for many non...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx078
更新日期:2017-11-01 00:00:00
abstract::Sialyltransferases are a family of 20 gene products in mice and humans that transfer sialic acid from its activated precursor, CMP-sialic acid, to the terminus of glycoprotein and glycolipid acceptors. ST3Gal-II (coded by the St3gal2 gene) transfers sialic acid preferentially to the three positions of galactose on the...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww098
更新日期:2017-01-01 00:00:00
abstract::Neisseria meningitidis (Nm) is a leading cause of bacterial meningitis and sepsis. A key feature in pathogenicity is the capsular polysaccharide (CPS) that prevents complement activation and thus supports bacterial survival in the host. Twelve serogroups characterized by immunologically and structurally different CPSs...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt102
更新日期:2014-02-01 00:00:00
abstract::In this paper, we present two complementary strategies for enrichment of glycoproteins on living cells that combine the desirable attributes of "robust enrichment" afforded by covalent-labeling techniques and "specificity for glycoproteins" typically provided by lectin or antibody affinity reagents. Our strategy invol...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws144
更新日期:2013-02-01 00:00:00
abstract::The oligosaccharide chains (glycans) attached to cell surface and extracellular proteins and lipids are known to mediate many important biological roles. However, for many glycans, there are still no evident functions that are of obvious benefit to the organism that synthesizes them. There is also no clear explanation...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/9.8.747
更新日期:1999-08-01 00:00:00
abstract::We have characterized a glycosphingolipid (GSL) receptor for Staphylococcus enterotoxin-B (SEB) in cultured human kidney proximal tubular (PT) cells. Solid-phase binding of [125I]SEB to the GSL receptor was concentration dependent and was not displaceable by two structurally related toxins, such as staphylococcal ente...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.3.327
更新日期:1995-05-01 00:00:00
abstract::In the yeast Saccharomyces cerevisiae, the alg2 mutation causes temperature-sensitive growth and abnormal accumulation of the lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol (Jackson et al., Arch. Biochem. Biophys., 272, 203-209, 1989; Huffaker and Robbins, Proc. Natl. Acad. Sci. USA, 80, 7466-7470, 1983). A gene havi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.4.357
更新日期:1993-08-01 00:00:00
abstract::We have recently demonstrated that the mannose-binding lectins, namely banana lectin (BL) and garlic lectin (GL), interacted with the insulin receptors on M210B4 cells--an established mesenchymal cell line of murine marrow origin--and initiate mitogen-activated protein kinase kinase (MEK)-dependent extracellular signa...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq194
更新日期:2011-04-01 00:00:00
abstract::Human milk oligosaccharides (HMOS) are not digested in the proximal intestine. In distal intestine, HMOS collectively modify the microbiota, but the response of individual bacteria to individual components of the HMOS is not well defined. Here, each of 25 major isolates of the human intestinal microbiota was fed indiv...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt065
更新日期:2013-11-01 00:00:00
abstract::Serglycin with a green fluorescent protein tag (SG-GFP) expressed in epithelial Madin-Darby canine kidney cells is secreted mainly (85%) into the apical medium, but the glycosaminoglycan (GAG) chains on the SG-GFP protein core secreted basolaterally (15%) carry most of the sulfate added during biosynthesis (Tveit et a...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj075
更新日期:2006-04-01 00:00:00
abstract::Xanthomonas translucens pv. translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae. The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized. The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww093
更新日期:2017-03-15 00:00:00
abstract::Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion. The activity was solubilized by t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/1.6.605
更新日期:1991-12-01 00:00:00
abstract::The gut microbiota plays a major role in human health and an alteration in gut microbiota structure and function has been implicated in several diseases. In the colon, mucus covering the epithelium is critical to maintain a homeostatic relationship with the gut microbiota by harbouring a microbial community at safe di...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwaa097
更新日期:2020-10-09 00:00:00
abstract::Trypanosoma cruzi expresses a unique trans-sialidase that is responsible for the transfer of sialic acid from host glycoproteins and glycolipids to mucin-like glycoprotein acceptors on the parasite surface. The enzyme and the sialic acid acceptors are present in the mammalian forms of the parasite and in the parasite ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.6.625
更新日期:1995-09-01 00:00:00
abstract::Mechanisms for nonenzymatic protein glycation have been extensively studied albeit with an emphasis at the later stages that gives rise to advanced glycation end products. No detailed investigation of the initial, noncovalent binding of d-glucose to human hemoglobin A (HbA) exists in the literature. Although anionic m...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt061
更新日期:2013-11-01 00:00:00
abstract::Artocarpus lakoocha agglutinin (ALA), isolated from the seeds of A. lakoocha fruit, is a galactose-binding lectin and a potent mitogen of T and B cells. Knowledge obtained from previous studies on the affinity of ALA was limited to molecular and submolecular levels of Galbeta1-->3GalNAc (T) and its derivatives. In the...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh144
更新日期:2005-01-01 00:00:00
abstract::Presently almost no information is available on the oligosaccharide structure of the glycoproteins secreted from the methylotrophic yeast Hansenula polymorpha, a promising host for the production of recombinant proteins. In this study, we analyze the size distribution and structure of N-linked oligosaccharides attache...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh030
更新日期:2004-03-01 00:00:00
abstract::Mouse gene knockout studies have provided unimpeachable evidence of immune-relevant functions for several sialyltransferase enzymes including ST6Gal I, ST3Gal I, and ST3Gal IV. Such studies cannot, however, identify cellular mechanisms for regulating such activities. In this article we provide evidence that murine B l...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh133
更新日期:2004-12-01 00:00:00