Abstract:
:The presence of nonmammalian core alpha(1,3)-fucose and core xylose glyco-epitopes on glycans N-linked to therapeutic glycoproteins produced in plants has raised the question of their immunogenicity in human therapy. We address this question by studying the distribution of these N-glycans in pea, rice, and maize (which are the crops intended for the production of therapeutic proteins) and by reinvestigating their immunogenicity in rodents. We found that immunization with a model glycoprotein, horseradish peroxidase, elicits in C57BL/6 mice and rats the production of antibodies (Abs) specific for core alpha(1,3)-fucose and core xylose epitopes. Furthermore, we demonstrated that about 50% of nonallergic blood donors contains in their sera Abs specific for core xylose, whereas 25% have Abs against core alpha(1,3)-fucose. These Abs probably result from sensitization to environmental antigens. Although the immunological significance of these data is too speculative at the moment, the presence of such Abs might introduce some limitations to the use of plant-derived biopharmaceutical glycoproteins, such as an accelerated clearance during human therapy.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Bardor M,Faveeuw C,Fitchette AC,Gilbert D,Galas L,Trottein F,Faye L,Lerouge Pdoi
10.1093/glycob/cwg024subject
Has Abstractpub_date
2003-06-01 00:00:00pages
427-34issue
6eissn
0959-6658issn
1460-2423pii
cwg024journal_volume
13pub_type
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