Abstract:
:The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The N- glycan structures were conclusively determined in terms of complexity and degree of fucosylation. Ion-exchange chromatography with pulsed amperometric detection, together with mass-spectral analysis of the esterified N- glycans, indicated the presence of monosialylated structures. The molecular mass profile of esterified N- glycans present in BSSL further permitted the more detailed studies through collision-induced dissociation (CID) and sequential exoglycosidase cleavages. The N- glycan structures were elucidated to be complex/dibranched, fucosylated/complex/dibranched, monosialylated/complex/dibranched, and monosialylated/fucosylated/dibranched entities.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Mechref Y,Chen P,Novotny MVdoi
10.1093/glycob/9.3.227subject
Has Abstractpub_date
1999-03-01 00:00:00pages
227-34issue
3eissn
0959-6658issn
1460-2423pii
cwc019journal_volume
9pub_type
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