Abstract:
:Glycans mediate a wide variety of biological roles via recognition by glycan-binding proteins (GBPs). Comprehensive knowledge of such interaction is thus fundamental to glycobiology. While the primary binding feature of GBPs can be easily uncovered by using a simple glycan microarray harboring limited numbers of glycan motifs, their fine specificities are harder to interpret. In this study, we prepared 98 closely related N-glycoforms that contain 5 common glycan epitopes which allowed the determination of the fine binding specificities of several plant lectins and anti-glycan antibodies. These N-glycoforms differ from each other at the monosaccharide level and were presented in an identical format to ensure comparability. With the analysis platform we used, it was found that most tested GBPs have preferences toward only one branch of the complex N-glycans, and their binding toward the epitope-presenting branch can be significantly affected by structures on the other branch. Fine specificities described here are valuable for a comprehensive understanding and applications of GBPs.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Li L,Guan W,Zhang G,Wu Z,Yu H,Chen X,Wang PGdoi
10.1093/glycob/cwz100subject
Has Abstractpub_date
2020-04-20 00:00:00pages
334-345issue
5eissn
0959-6658issn
1460-2423pii
5663452journal_volume
30pub_type
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