Glycobiology of neuromuscular disorders.

abstract：:Galactans are linear polysaccharides of β(1→4)-linked galactose residues. Although they can antagonize galectin function, the nature of their binding to galectins needs to be better defined to develop them as drugs. Here, we investigated interactions between galectin-3 (Gal-3) and a series of galactans ranging in weig...

journal_title：Glycobiology

authors： Miller MC,Zheng Y,Zhou Y,Tai G,Mayo KH

更新日期：2019-01-01 00:00:00

abstract：:It is demonstrated with glycopeptides of the polymorphic epithelial mucin (MUC1) that post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) is a fast, highly sensitive, and reproducible method for the localization of O-glycosylation sites by reflectron time-of-flight (TOF) mass spectrometry. We hav...

journal_title：Glycobiology

authors： Goletz S,Thiede B,Hanisch FG,Schultz M,Peter-Katalinic J,Müller S,Seitz O,Karsten U

更新日期：1997-10-01 00:00:00

abstract：:The structure of a antigen-binding fragment (Fab) from the bactericidal monoclonal antibody LPT3-1 specific to lipooligosaccharide (LOS) inner cores from Neisseria meningitidis has been solved in complex with an eight-sugar inner core fragment NmL3 galE lpt3 KOH to 2.69 Å resolution. The epitope is centered about an i...

journal_title：Glycobiology

authors： Parker MJ,Gomery K,Richard G,MacKenzie CR,Cox AD,Richards JC,Evans SV

更新日期：2014-05-01 00:00:00

abstract：:Intelectin is an extracellular animal lectin found in chordata. Although human and mouse intelectin-1 recognize galactofuranosyl residues included in cell walls of various microorganisms, the physiological function of mammalian intelectin had been unclear. In this study, we found that human intelectin-1 was a serum pr...

journal_title：Glycobiology

authors： Tsuji S,Yamashita M,Hoffman DR,Nishiyama A,Shinohara T,Ohtsu T,Shibata Y

更新日期：2009-05-01 00:00:00

abstract：:Heparan sulfate is a polysaccharide belonging to the glycaminoglycan family. It interacts with numerous proteins of the extracellular matrix, in particular cellular growth factors. The number of experimental protein-heparin sulfate complexes obtained by crystallography or nuclear magnetic resonance is limited. Alterna...

journal_title：Glycobiology

authors： Sapay N,Cabannes E,Petitou M,Imberty A

更新日期：2011-09-01 00:00:00

abstract：:Glycopeptides representing individual N-glycosylation sites of the heterodimeric glycoprotein hormone human chorionic gonadotrophin (hCG) were obtained from subunits hCG alpha (N-glycosylated at Asn-52 and Asn-78) and hCG beta (N-glycosylated at Asn-13 and Asn-30) by digestion with trypsin and chymotrypsin, respective...

journal_title：Glycobiology

authors： Weisshaar G,Hiyama J,Renwick AG

更新日期：1991-09-01 00:00:00

abstract：:Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. Today, only one recombinant human Epo (rHuEpo), produced in CHO cell line, is extensively used in t...

journal_title：Glycobiology

authors： Cointe D,Béliard R,Jorieux S,Leroy Y,Glacet A,Verbert A,Bourel D,Chirat F

更新日期：2000-05-01 00:00:00

abstract：:To study human alpha1,3/1,4fucosyltransferase (Fuc-TIII) as an alpha1,3 fucosyltransferase, we constructed two cell clones, C127-FT and C127-T-FT, by transfecting cDNA in parental (C127) or Polyoma T antigen expressing (C127-T) mouse cells, respectively. Both C127-FT and C127-T-FT clones express high levels of a fucos...

journal_title：Glycobiology

authors： Valli M,Bardoni A,Trinchera M

更新日期：1999-01-01 00:00:00

abstract：:In this work, focused on possible application of α-L-fucosidases from bacterial sources in the synthesis of α-L-fucosylated glycoconjugates, several nonpathogenic aerobic bacterial strains were screened for α-L-fucosidase activity. Among them Paenibacillus thiaminolyticus was confirmed as a potent producer of enzyme w...

journal_title：Glycobiology

authors： Benesová E,Lipovová P,Dvoráková H,Králová B

更新日期：2013-09-01 00:00:00

abstract：:The interaction of heparan sulfate (HS) (and the closely related molecule heparin) with FGF-1 is a requirement for enabling the growth factor to activate its cell surface tyrosine kinase receptor. However, little is known about the regulatory role of naturally occurring cell surface HS in FGF-1 activation. We have add...

journal_title：Glycobiology

authors： Pye DA,Vivès RR,Hyde P,Gallagher JT

更新日期：2000-11-01 00:00:00

abstract：:Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...

journal_title：Glycobiology

authors： Takahashi S,Sasaki T,Manya H,Chiba Y,Yoshida A,Mizuno M,Ishida H,Ito F,Inazu T,Kotani N,Takasaki S,Takeuchi M,Endo T

更新日期：2001-01-01 00:00:00

abstract：:Gangliosides are able to bind to the epidermal growth factor receptor and inhibit its activation, but the mechanism of this inhibition is unknown. To address the role of receptor carbohydrates in facilitating interaction with gangliosides, we examined the ability of GM3 to bind the deglycosylated receptor and inhibit ...

journal_title：Glycobiology

authors： Wang XQ,Sun P,O'Gorman M,Tai T,Paller AS

更新日期：2001-07-01 00:00:00

abstract：:The methylotrophic yeast, Pichia pastoris, is an important organism used for the production of therapeutic proteins. However, the presence of fungal-like glycans, such as those containing β-mannose (Man) linkages, can elicit an immune response or bind to Man receptors, thus reducing their efficacy. Recent studies have...

journal_title：Glycobiology

authors： Hopkins D,Gomathinayagam S,Rittenhour AM,Du M,Hoyt E,Karaveg K,Mitchell T,Nett JH,Sharkey NJ,Stadheim TA,Li H,Hamilton SR

更新日期：2011-12-01 00:00:00

abstract：:Xanthomonas translucens pv. translucens (Xtt) is a Gram-negative pathogen of crops from the plant family Poaceae. The lipopolysaccharide (LPS) of Xtt was isolated and chemically characterized. The analyses revealed the presence of rhamnose, xylose, mannose, glucose, galacturonic acid, phosphates, 3-deoxy-D-manno-oct-2...

journal_title：Glycobiology

authors： Steffens T,Duda K,Lindner B,Vorhölter FJ,Bednarz H,Niehaus K,Holst O

更新日期：2017-03-15 00:00:00

abstract：:Circulatory protein glycosylation is a biomarker of multiple disease and inflammatory states and has been applied in the clinic for liver dysfunction, heart disease and diabetes. With the notable exception of antibodies, the liver produces most of the circulatory glycoproteins, including the acute phase proteins relea...

journal_title：Glycobiology

authors： Oswald DM,Jones MB,Cobb BA

更新日期：2020-04-20 00:00:00

abstract：:Apiose is a unique branched-chain pentose found principally in plants. It is a key component of structurally complex cell wall polysaccharides, as well as being present in a large number of naturally occurring secondary metabolites. This review provides a comprehensive overview of the current state of knowledge on the...

journal_title：Glycobiology

authors： Pičmanová M,Møller BL

更新日期：2016-05-01 00:00:00

abstract：:To date, there is no complete structural characterization of human milk glycosaminoglycans (GAGs) available nor do any data exist on their composition in bovine milk. Total GAGs were determined on extracts from human and bovine milk. Samples were subjected to digestion with specific enzymes, treated with nitrous acid,...

journal_title：Glycobiology

authors： Coppa GV,Gabrielli O,Buzzega D,Zampini L,Galeazzi T,Maccari F,Bertino E,Volpi N

更新日期：2011-03-01 00:00:00

abstract：:P3 is a mouse monoclonal antibody (mAb) that binds to several NeuGc-containing gangliosides. It also reacts with antigens expressed in human breast tumors (Vázquez et al. (1995) Hybridoma , 14, 551-556). In this work, the binding specificity of P3 has been characterized in more detail using a panel of glycolipids that...

journal_title：Glycobiology

authors： Moreno E,Lanne B,Vázquez AM,Kawashima I,Tai T,Fernández LE,Karlsson KA,Angström J,Pérez R

更新日期：1998-07-01 00:00:00

abstract：:The endoplasmic reticulum malectin is a highly conserved protein in the animal kingdom that has no counterpart so far in lower organisms. We recently determined the structure of its conserved domain and found a highly selective binding to Glc(2)Man(9)GlcNAc(2), an intermediate of N-glycosylation. In our quest for puta...

journal_title：Glycobiology

authors： Schallus T,Fehér K,Sternberg U,Rybin V,Muhle-Goll C

更新日期：2010-08-01 00:00:00

abstract：:Recombinant human erythropoietin (rHuEPO) is used abundantly in the clinic to stimulate red blood cell growth in anaemic patients. The efficacy of the drug depends strongly on the extent of sialylation of its carbohydrate moiety. Prompted by conflicting literature reports on the issue, we reinvestigated the structures...

journal_title：Glycobiology

authors： Watson E,Bhide A,van Halbeek H

更新日期：1994-04-01 00:00:00

abstract：:The mucin-type O-glycome in cancer aberrantly expresses the truncated glycans Tn (GalNAcα1-Ser/Thr) and STn (Neu5Acα2,6GalNAcα1-Ser/Thr). However, the role of Tn and STn in cancer and other diseases is not well understood. Our recent discovery of the self-binding properties (carbohydrate-carbohydrate interactions, CCI...

journal_title：Glycobiology

authors： Sletmoen M,Gerken TA,Stokke BT,Burchell J,Brewer CF

更新日期：2018-07-01 00:00:00

abstract：:Although members of the serine (S)- and arginine (R)-rich splicing factor family (SR proteins) were initially purified on the basis of their splicing activity in the nucleus, there is recent documentation that they exhibit carbohydrate-binding activity at the cell surface. In contrast, galectins were isolated on the b...

journal_title：Glycobiology

authors： Haudek KC,Patterson RJ,Wang JL

更新日期：2010-10-01 00:00:00

abstract：:Galbeta1-4GlcNAc is thought to be a common disaccharide unit preferentially recognized by vertebrate galectins. Eight-amino-acid residues conserved in proteins belonging to the galectin family have been suggested to be responsible for recognition. Meanwhile, we isolated and analyzed endogenous N-glycans of Caenorhabdi...

journal_title：Glycobiology

authors： Takeuchi T,Nishiyama K,Sugiura K,Takahashi M,Yamada A,Kobayashi S,Takahashi H,Natsugari H,Kasai K

更新日期：2009-12-01 00:00:00

abstract：:The detailed structures of N- glycans derived from bile salt-stimulated lipase (BSSL) found in human milk were determined by combining exoglycosidase digestion with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The N- glycan structures were conclusively determined in terms of complexity...

journal_title：Glycobiology

authors： Mechref Y,Chen P,Novotny MV

更新日期：1999-03-01 00:00:00

abstract：:The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it i...

journal_title：Glycobiology

authors： Kanda Y,Yamada T,Mori K,Okazaki A,Inoue M,Kitajima-Miyama K,Kuni-Kamochi R,Nakano R,Yano K,Kakita S,Shitara K,Satoh M

更新日期：2007-01-01 00:00:00

abstract：:F-type lectins are fucolectins with characteristic fucose and calcium-binding sequence motifs and a unique lectin fold (the "F-type" fold). F-type lectins are phylogenetically widespread with selective distribution. Several eukaryotic F-type lectins have been biochemically and structurally characterized, and the F-typ...

journal_title：Glycobiology

authors： Bishnoi R,Khatri I,Subramanian S,Ramya TN

更新日期：2015-08-01 00:00:00

abstract：:Lipid-A represents the ubiquitous, covalently bound hydrophobic component of bacterial lipopolysaccharides (endotoxins). Lipid-As isolated and characterized from rhizobial species have large variations in their backbone sugars, as well as in their hydroxy fatty acid substituents. The sugar backbones consist of either ...

journal_title：Glycobiology

authors： Bhat UR,Carlson RW

更新日期：1992-12-01 00:00:00

abstract：:Glycoinositolphospholipids (GIPLs) were isolated from promastigotes of the lizard parasites Leishmania adleri by phenol/water extraction. Phosphoinositol oligosaccharides were liberated by mild alkaline hydrolysis, purified by gel filtration and high pH anion exchange chromatography, and characterized by methylation a...

journal_title：Glycobiology

authors： Previato JO,Jones C,Wait R,Routier F,Saraiva E,Mendonça-Previato L

更新日期：1997-07-01 00:00:00

abstract：:Gangliosides (sialic acid-containing glycosphingolipids) play important roles in many physiological functions, including synaptic plasticity in the hippocampus, which is considered as a cellular mechanism of learning and memory. In the present study, three types of synaptic plasticity, long-term potentiation (LTP), lo...

journal_title：Glycobiology

authors： Ikarashi K,Fujiwara H,Yamazaki Y,Goto J,Kaneko K,Kato H,Fujii S,Sasaki H,Fukumoto S,Furukawa K,Waki H,Furukawa K

更新日期：2011-10-01 00:00:00

abstract：:Lepidopteran insect cells are used routinely as hosts for foreign glycoprotein expression by recombinant baculoviruses, but the precise nature of their N-glycosylation pathway remains poorly defined. These cells clearly have processing glucosidases and mannosidases that can convert precursors to Man3GlcNAc2 structures...

journal_title：Glycobiology

authors： Jarvis DL,Bohlmeyer DA,Liao YF,Lomax KK,Merkle RK,Weinkauf C,Moremen KW

更新日期：1997-02-01 00:00:00