Abstract:
:F-type lectins are fucolectins with characteristic fucose and calcium-binding sequence motifs and a unique lectin fold (the "F-type" fold). F-type lectins are phylogenetically widespread with selective distribution. Several eukaryotic F-type lectins have been biochemically and structurally characterized, and the F-type lectin domain (FLD) has also been studied in the bacterial proteins, Streptococcus mitis lectinolysin and Streptococcus pneumoniae SP2159. However, there is little knowledge about the extent of occurrence of FLDs and their domain organization, especially, in bacteria. We have now mined the extensive genomic sequence information available in the public databases with sensitive sequence search techniques in order to exhaustively survey prokaryotic and eukaryotic FLDs. We report 437 FLD sequence clusters (clustered at 80% sequence identity) from eukaryotic, eubacterial and viral proteins. Domain architectures are diverse but mostly conserved in closely related organisms, and domain organizations of bacterial FLD-containing proteins are very different from their eukaryotic counterparts, suggesting unique specialization of FLDs to suit different requirements. Several atypical phylogenetic associations hint at lateral transfer. Among eukaryotes, we observe an expansion of FLDs in terms of occurrence and domain organization diversity in the taxa Mollusca, Hemichordata and Branchiostomi, perhaps coinciding with greater emphasis on innate immune strategies in these organisms. The naturally occurring FLDs with diverse domain organizations that we have identified here will be useful for future studies aimed at creating designer molecular platforms for directing desired biological activities to fucosylated glycoconjugates in target niches.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Bishnoi R,Khatri I,Subramanian S,Ramya TNdoi
10.1093/glycob/cwv029subject
Has Abstractpub_date
2015-08-01 00:00:00pages
888-901issue
8eissn
0959-6658issn
1460-2423pii
cwv029journal_volume
25pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::We have generated a monoclonal antibody (R-10G) specific to human induced pluripotent stem (hiPS)/embryonic stem (hES) cells by using hiPS cells (Tic) as an antigen, followed by differential screening of mouse hybridomas with hiPS and human embryonal carcinoma (hEC) cells. Upon western blotting with R-10G, hiPS/ES cel...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws159
更新日期:2013-03-01 00:00:00
abstract::The hexosamine pathway (HP) is a biochemical hypothesis recently proposed explaining cellular alterations occurring during diabetic microvascular complications. Diabetic retinopathy is a common microvascular complication of diabetes, and it is known that cell proliferation is severely affected during the development o...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi039
更新日期:2005-06-01 00:00:00
abstract::Gangliosides are able to bind to the epidermal growth factor receptor and inhibit its activation, but the mechanism of this inhibition is unknown. To address the role of receptor carbohydrates in facilitating interaction with gangliosides, we examined the ability of GM3 to bind the deglycosylated receptor and inhibit ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.7.515
更新日期:2001-07-01 00:00:00
abstract::The biosynthesis of HNK-1 carbohydrate is mainly regulated by two glucuronyltransferases (GlcAT-P and GlcAT-S) and a sulfotransferase (HNK-1 ST). To determine how the two glucuronyltransferases are involved in the biosynthesis of the HNK-1 carbohydrate, we prepared soluble forms of GlcAT-P and GlcAT-S fused with the I...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi001
更新日期:2005-02-01 00:00:00
abstract::Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz061
更新日期:2019-11-20 00:00:00
abstract::The product of p53-induced gene 1 is a member of the galectin family, i.e., galectin-7 (Gal-7). To move beyond structural data by X-ray diffraction, we initiated the study of the lectin by nuclear magnetic resonance (NMR) and circular dichroism spectroscopies, and molecular dynamics (MD) simulations. In concert, our r...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt005
更新日期:2013-05-01 00:00:00
abstract::We have examined the time course of the neuronal death and regeneration of rat axotomized hypoglossal nerve with various conditions of the nerve resection, and established a useful system to measure neurotrophic activities of bioactive substances. In this system, neuronal death can be evaluated by counting surviving n...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.11.1247
更新日期:1999-11-01 00:00:00
abstract::Heparan sulfate 3-O-sulfotransferase transfers sulfate to the 3-OH position of a glucosamine to generate 3-O-sulfated heparan sulfate (HS), which is a rare component in HS from natural sources. We previously reported that 3-O- sulfotransferase isoform 5 (3-OST-5) generates both an antithrombin-binding site to exhibit ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg101
更新日期:2003-11-01 00:00:00
abstract::Mouse macrophage galactose/N-acetylgalactosamine-specific C-type lectin (MMGL) is a type II transmembrane glycoprotein belonging to the C-type lectin family. Our development of monoclonal antibodies led us to discover that a calcium-dependent conformational change is detected by an antibody (termed mAb LOM-11) and tha...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.8.791
更新日期:1998-08-01 00:00:00
abstract::Lectins are used as defense effector proteins against predators, parasites and pathogens by animal, plant and fungal innate defense systems. These proteins bind to specific glycoepitopes on the cell surfaces and thereby interfere with the proper cellular functions of the various antagonists. The exact cellular toxicit...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww113
更新日期:2017-05-01 00:00:00
abstract::In Campylobacter jejuni, the PglB oligosaccharyltransferase catalyzes the transfer of a heptasaccharide from a lipid donor to asparagine within the D/E-X1-N-X2-S/T sequon (X1,2 ≠ P) or releases this heptasaccharide as free oligosaccharides (fOS). Using available crystal structures and sequence alignments, we identifie...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx067
更新日期:2017-10-01 00:00:00
abstract::The high-molecular-mass salivary mucin MG1, one of two major mucins produced by human salivary glands, plays an important role in oral health by coating the tooth surface and by acting as a bacterial receptor. Here this mucin was purified from the submandibular/sublingual saliva of a blood group O individual. The pres...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/12.1.1
更新日期:2002-01-01 00:00:00
abstract::Galectin-1, the prototype of a family of beta-galactoside-binding proteins, has been implicated in a wide variety of biological processes. Data presented herein show that galectin-1 stimulates monocyte migration in a dose-dependent manner but is not chemotactic for macrophages. Galectin-1-induced monocyte chemotaxis i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp077
更新日期:2009-12-01 00:00:00
abstract::Glycoside hydrolases are particularly abundant in all areas of metabolism as they are involved in the degradation of natural polysaccharides and glycoconjugates. These enzymes are classified into 133 families (CAZy server, http://www.cazy.org) in which members of each family have a similar structure and catalytic mech...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu121
更新日期:2015-04-01 00:00:00
abstract::Endogenous lectins can control critical biological responses, including cell communication, signaling, angiogenesis and immunity by decoding glycan-containing information on a variety of cellular receptors and the extracellular matrix. Galectin-1 (Gal-1), a prototype member of the galectin family, displays only one ca...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww052
更新日期:2016-12-01 00:00:00
abstract::Bifidobacteria are predominant in the intestines of breast-fed infants and offer health benefits to the host. Human milk oligosaccharides (HMOs) are considered to be one of the most important growth factors for intestinal bifidobacteria. HMOs contain two major structures of core tetrasaccharide: lacto-N-tetraose (Galβ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq101
更新日期:2010-11-01 00:00:00
abstract::Glycosaminoglycan (GAG) assembly initiates through the formation of a linkage tetrasaccharide region serving as a primer for both chondroitin sulfate (CS) and heparan sulfate (HS) chain polymerization. A possible role for sulfation of the linkage structure and of the constitutive disaccharide unit of CS chains in the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr172
更新日期:2012-04-01 00:00:00
abstract::Beta-1,2-linked mannosides from Candida albicans phosphopeptidomannan (PPM) bind to macrophages through a receptor independent from the macrophage alpha-linked mannose receptor and stimulate these cells to secrete immune mediators. Anti-beta-1,2-linked mannoside but not anti-alpha-linked mannoside antibodies produced ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.8.693
更新日期:2001-08-01 00:00:00
abstract::We report the production of biologically active hyaluronan (HA) oligosaccharides labeled with the fluorophore 2-aminobenzoic acid (2AA). Oligosaccharides from 4 to 40 residues in length were purified to homogeneity by ion exchange chromatography using a logarithmic gradient. Molecular weight and purity characterizatio...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi008
更新日期:2005-03-01 00:00:00
abstract::We recently described a novel fluorescent compound, 2-amino,6-amidobiotinyl-pyridine (BAP), that allows the tagging of oligosaccharides, their fractionation by reversed-phase HPLC with picomole scale detection, and the formation of functional neoglycoprotein equivalents with (strept) avidin for the detection of recept...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.5.653
更新日期:1994-10-01 00:00:00
abstract::N-acetylneuraminic acid (5-acetamido-3,5-dideoxy-d-glycero-d-galacto-non-2-ulosonic acid), which is the principal sialic acid family member of the non-2-ulosonic acids and their various derivatives, is often found at the terminal position on the glycan chains that adorn all vertebrate cells. This terminal position com...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz099
更新日期:2020-04-20 00:00:00
abstract::The rat liver sinusoidal endothelial cell (LEC) hyaluronan (HA) receptor was previously identified using a photoaffinity HA derivative (J. Biol. Chem., 267, 20451-20456, 1992). Two polypeptides with M(r) = 175,000 and 166,000, were consistently crosslinked, suggesting that the LEC HA receptor is an oligomer. Whether o...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.1.15
更新日期:1997-02-01 00:00:00
abstract::A rat pheochromocytoma cell line (PC12), when transfected with beta1,4-N-acetylglucosaminyltransferase III (GnT-III), which catalyzes the formation of a bisecting GlcNAc structure in N-glycans, resulted in the suppression of neurite outgrowth induced by costimulation of epidermal growth factor (EGF) and integrins. The...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh016
更新日期:2004-02-01 00:00:00
abstract::The cDNAs that encode the 70 kDa C-terminal portion of Plasmodium falciparum merozoite surface protein 1 (MSP-1), with or without an N-terminal signal peptide sequence and C-terminal glycosylphosphatidylinositol (GPI) signal sequence of MSP-1, were expressed in mammalian cell lines via recombinant vaccinia virus. The ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/9.12.1347
更新日期:1999-12-01 00:00:00
abstract::Human alpha-galactosidase A (alpha-Gal A) is the lysosomal glycohydrolase that cleaves the terminal alpha-galactosyl moieties of various glycoconjugates. Overexpression of the enzyme in Chinese hamster ovary (CHO) cells results in high intracellular enzyme accumulation and the selective secretion of active enzyme. Str...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.4.329
更新日期:1998-04-01 00:00:00
abstract::The structure of a antigen-binding fragment (Fab) from the bactericidal monoclonal antibody LPT3-1 specific to lipooligosaccharide (LOS) inner cores from Neisseria meningitidis has been solved in complex with an eight-sugar inner core fragment NmL3 galE lpt3 KOH to 2.69 Å resolution. The epitope is centered about an i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu009
更新日期:2014-05-01 00:00:00
abstract::Several tri- to hexasaccharide-alditols of the jelly coat surrounding the eggs of Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and 1H-NMR spectroscopy. As observed for six other amphibian species, these carbohydrate chains are highly species-specific. The main characteristics of the spe...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.6.777
更新日期:1997-09-01 00:00:00
abstract::N-acetylglucosaminyltransferase V, encoded by the Mgat5 gene, plays an important regulatory role in the synthesis of complex N-glycans, including the Lewis antigens. The elevated expression of this enzyme is regulated during development and is highly associated with cellular transformation and malignancy. In addition,...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwf040
更新日期:2002-07-01 00:00:00
abstract::The enzyme UDP-N-acetylglucosamine: α-d-mannoside β-1-6 N-acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of GlcNAc from the UDP-GlcNAc donor to the α-1-6-linked mannose of the trimannosyl core structure of glycoproteins to produce the β-1-6-linked branching of N-linked oligosaccharides. β-1-6-GlcNAc-br...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww010
更新日期:2016-07-01 00:00:00
abstract::Protein O-fucosyltransferase 1 (Pofut1) and protein O-fucosyltransferase 2 (Pofut2) add O-linked fucose at distinct consensus sequences in properly folded epidermal growth factor (EGF)-like repeats and thrombospondin type-1 (TSR) repeats, respectively. Glycan chain elongation past O-fucose can occur to yield a tetrasa...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws140
更新日期:2013-02-01 00:00:00