Abstract:
:The enzyme UDP-N-acetylglucosamine: α-d-mannoside β-1-6 N-acetylglucosaminyltransferase V (GnT-V) catalyzes the transfer of GlcNAc from the UDP-GlcNAc donor to the α-1-6-linked mannose of the trimannosyl core structure of glycoproteins to produce the β-1-6-linked branching of N-linked oligosaccharides. β-1-6-GlcNAc-branched N-glycans are associated with cancer growth and metastasis. Therefore, the inhibition of GnT-V represents a key target for anti-cancer drug development. However, the development of potent and specific inhibitors of GnT-V is hampered by the lack of information on the three-dimensional structure of the enzyme and on the binding characteristics of its substrates. Here we present the first 3D structure of GnT-V as a result of homology modeling. Various alignment methods, docking the donor and acceptor substrates, and molecular dynamics simulation were used to construct seven homology models of GnT-V and characterize the binding of its substrates. The best homology model is consistent with available experimental data. The three-dimensional model, the structure of the enzyme catalytic site and binding information obtained for the donor and acceptor can be useful in studies of the catalytic mechanism and design of inhibitors of GnT-V.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Janoš P,Kozmon S,Tvaroška I,Koca Jdoi
10.1093/glycob/cww010subject
Has Abstractpub_date
2016-07-01 00:00:00pages
757-771issue
7eissn
0959-6658issn
1460-2423pii
cww010journal_volume
26pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::Antisera raised against galectin-1 exhibit crossreactivities with other galectins or related molecules. In order to overcome this problem, a monoclonal antibody to human brain galectin-1 was obtained by selecting clones without reactivity toward galectin-3. This mAb specifically bound galectin-1 of various animal orig...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.5.425
更新日期:1998-05-01 00:00:00
abstract::The Neu1 locus, in the S region of the murine histocompatibility-2 complex, regulates the sialic acid content of several liver lysosomal enzymes. Three alleles, Neu1a, Neu1b, and Neu1c, have been described on the basis of differential sialylation of the enzyme liver acid phosphatase. The Neu1a allele occurs in a small...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.7.975
更新日期:1997-10-01 00:00:00
abstract::It is demonstrated with glycopeptides of the polymorphic epithelial mucin (MUC1) that post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) is a fast, highly sensitive, and reproducible method for the localization of O-glycosylation sites by reflectron time-of-flight (TOF) mass spectrometry. We hav...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.7.881
更新日期:1997-10-01 00:00:00
abstract::Several tri- to hexasaccharide-alditols of the jelly coat surrounding the eggs of Bufo bufo were studied by methylation analysis, MALDI-TOF mass spectrometry, and 1H-NMR spectroscopy. As observed for six other amphibian species, these carbohydrate chains are highly species-specific. The main characteristics of the spe...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.6.777
更新日期:1997-09-01 00:00:00
abstract::From a systematic search of the UniGene and dbEST databanks, using human beta 4-galactosyltransferase (beta 4GalT-I), which is recognized to function in lactose biosynthesis, as the query sequence, we have identified five additional gene family members denoted as beta 4GalT-II, -III, -IV, -V, and -VI. Complementary DN...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.5.517
更新日期:1998-05-01 00:00:00
abstract::Epithelial cells in the lining of the intestines play critical roles in maintaining homeostasis while challenged by dynamic and sudden changes in luminal contents. Given the high density of glycosylation that encompasses their extracellular surface, environmental changes may lead to extensive reorganization of membran...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx041
更新日期:2017-09-01 00:00:00
abstract::The binding properties of Pseudomonas aeruginosa agglutinin-I (PA-IL) with glycoproteins (gps) and polysaccharides were studied by both the biotin/avidin-mediated microtiter plate lectin-binding assay and the inhibition of agglutinin-glycan interaction with sugar ligands. Among 36 glycans tested for binding, PA-IL rea...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.1.7
更新日期:1998-01-01 00:00:00
abstract::Bifidobacteria are predominant in the intestines of breast-fed infants and offer health benefits to the host. Human milk oligosaccharides (HMOs) are considered to be one of the most important growth factors for intestinal bifidobacteria. HMOs contain two major structures of core tetrasaccharide: lacto-N-tetraose (Galβ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwq101
更新日期:2010-11-01 00:00:00
abstract::alpha-1,4-Glucan lyase cleaves alpha-1,4-linkages of nonreducing termini of alpha-1,4-glucans to produce 1,5-anhydrofructose (1,5-AnFru). The enzymes isolated from fungi and algae show high homology with glycoside hydrolase family 31. Purification of alpha-1,4-glucan lyase from rat liver using DEAE Cellulose chromatog...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.12.1283
更新日期:2000-12-01 00:00:00
abstract::Mouse gene knockout studies have provided unimpeachable evidence of immune-relevant functions for several sialyltransferase enzymes including ST6Gal I, ST3Gal I, and ST3Gal IV. Such studies cannot, however, identify cellular mechanisms for regulating such activities. In this article we provide evidence that murine B l...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh133
更新日期:2004-12-01 00:00:00
abstract::There is increasing evidence that secretory fluids such as tears, saliva and milk play an important role in protecting the human body from infection via a washing mechanism involving glycan-mediated adhesion of potential pathogens to secretory glycoproteins. Interaction of sweat with bacteria is well established as th...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/cwv102
更新日期:2016-03-01 00:00:00
abstract::beta1-6 GlcNAc branching, a product of N-acetylglucosaminyltransferase V (GnT-V), is a key structure that is associated with malignant transformations and cancer metastasis. Although a number of reports concerning tumor metastasis-related glycoproteins that contain beta1-6 GlcNAc branching have appeared, the precise f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh013
更新日期:2004-02-01 00:00:00
abstract::We recently described a novel fluorescent compound, 2-amino,6-amidobiotinyl-pyridine (BAP), that allows the tagging of oligosaccharides, their fractionation by reversed-phase HPLC with picomole scale detection, and the formation of functional neoglycoprotein equivalents with (strept) avidin for the detection of recept...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.5.653
更新日期:1994-10-01 00:00:00
abstract::The acid alpha-mannosidase of Trypanosoma cruzi is a broad-specificity hydrolase involved in the catabolism of glycoconjugates, presumably in the digestive vacuole. We have cloned the alpha-mannosidase gene from a T.cruzi epimastigote genomic library. The alpha-mannosidase gene was determined to be single copy by Sout...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.12.1183
更新日期:1998-12-01 00:00:00
abstract::Detailed structural and functional studies over the last decade have led to current recognition of the mycobacterial lipoarabinomannan (LAM) as a phosphatidylinositol anchored lipoglycan with diverse biological activities. Fatty acylation has been demonstrated to be essential for LAM to maintain its functional integri...
journal_title:Glycobiology
pub_type: 杂志文章,评审
doi:10.1093/glycob/8.2.113
更新日期:1998-02-01 00:00:00
abstract::Human colon cancer is associated with antigenic and structural changes in mucin-type carbohydrate chains (O-glycans). To elucidate the control of the biosynthesis of these O-glycans is colon cancer, we have studied glycosyltransferase and sulphotransferase activities involved in the assembly of elongated O-glycan stru...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/4.6.873
更新日期:1994-12-01 00:00:00
abstract::Hyaluronan (HA) is a linear charged polysaccharide whose structure is made up of repeating disaccharide units. Apparently conflicting reports have been published about the nature of the helical structure of HA in the solid state. Recent developments in the field of molecular modeling of polysaccharides offer new oppor...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.6.587
更新日期:2000-06-01 00:00:00
abstract::Heparan sulfate/heparin N-deacetylase/N-sulfotransferase-1 (NDST-1) is a critical enzyme involved in heparan sulfate/heparin biosynthesis. This dual-function enzyme modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan. N-sulfation is an absolute requirement for the subsequent epi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh129
更新日期:2004-12-01 00:00:00
abstract::The structure of a antigen-binding fragment (Fab) from the bactericidal monoclonal antibody LPT3-1 specific to lipooligosaccharide (LOS) inner cores from Neisseria meningitidis has been solved in complex with an eight-sugar inner core fragment NmL3 galE lpt3 KOH to 2.69 Å resolution. The epitope is centered about an i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu009
更新日期:2014-05-01 00:00:00
abstract::Immunoglobulin G (IgG) glycans are emerging as a new putative biomarker for biological age and different diseases, requiring a robust workflow for IgG glycome analysis, ideally beginning with a simple and undemanding sampling procedure. Here, we report the first comprehensive study on total N-glycans of IgG isolated f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz061
更新日期:2019-11-20 00:00:00
abstract::A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh022
更新日期:2004-02-01 00:00:00
abstract::Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are primarily localized in the plasma membrane. Cholera toxin B subunit (Ctxb), a component of a heat-labile enterotoxin produced by Vibrio cholerae, has been frequently used as a probe to detect GM1 ganglio...
journal_title:Glycobiology
pub_type: 信件
doi:10.1093/glycob/cwl003
更新日期:2006-09-01 00:00:00
abstract::The structure of asparagine-linked oligosaccharides attached to the antibody constant region (Fc) of human immunoglobulin G1 (IgG1) has been shown to affect the pharmacokinetics and antibody effector functions of antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC). However, it i...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwl057
更新日期:2007-01-01 00:00:00
abstract::The concanavalin A staining of cellular glycoproteins and the direct analysis of their sugar chains released by hydrazinolysis revealed that the processing of N-linked sugar chains of some glycoproteins is suppressed by exposure of mouse monocytoid cells P388D1 to dimethyl sulphoxide, which can induce Fc receptor-medi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.1.15
更新日期:1993-02-01 00:00:00
abstract::Heparan sulfate is a polysaccharide belonging to the glycaminoglycan family. It interacts with numerous proteins of the extracellular matrix, in particular cellular growth factors. The number of experimental protein-heparin sulfate complexes obtained by crystallography or nuclear magnetic resonance is limited. Alterna...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr052
更新日期:2011-09-01 00:00:00
abstract::The structure of the O-antigen polysaccharide (PS) from the Shiga-toxin producing Escherichia coli O63 has been elucidated using a combination of bioinformatics, component analyses and NMR spectroscopy. The O-antigen is comprised of tetrasaccharide repeating units with the following structure: →2)-β-d-Quip3N(d-allo-Th...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy098
更新日期:2019-02-01 00:00:00
abstract::The Gram-negative bacterium Campylobacter jejuni 81116 (Penner serotype HS:6) has a class E lipooligosaccharide (LOS) biosynthesis locus containing 19 genes, which encode for 11 putative glycosyltransferases, 1 lipid A acyltransferase and 7 enzymes thought to be involved in the biosynthesis of dideoxyhexosamine (ddHex...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww136
更新日期:2017-04-01 00:00:00
abstract::Ganglioside GM3 (Siaα2-3Galβ1-4Glcβ1-1Cer) has been known to participate in insulin signaling by regulating the association of the insulin receptor in caveolae microdomains (lipid rafts), which is essential for the execution of the complete insulin metabolic signaling in adipocytes. Macrophage-secreted factors includi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu112
更新日期:2015-03-01 00:00:00
abstract::The role of aberrant protein modifications in cancer and its diagnosis have emerged as a promising research field. Nonenzymatic glyco-oxidation of proteins under oxidative stress has been associated with carcinogenesis through advanced glycation end products (AGE)-receptors for advanced glycation end products (RAGE) a...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwv082
更新日期:2016-02-01 00:00:00
abstract::Phytohaemagglutinin (PHA) is the major lectin present in the seeds of the common bean, Phaseolus vulgaris, and PHA-L is the leucocyte-agglutinating form of this lectin. This tetrameric glycoprotein accumulates in the vacuoles of storage parenchyma cells. Based on amino acid sequence comparisons of legume lectins and t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.6.581
更新日期:1993-12-01 00:00:00