Enterocyte glycosylation is responsive to changes in extracellular conditions: implications for membrane functions.

abstract：:Algal fucoidan is an alpha-L-fucose-based polysaccharide endowed with important biological properties for which the structure has not yet been fully elucidated. In an attempt to implement new enzymatic tools for structural study of this polysaccharide, we have found a fucosidase activity in the digestive glands of the...

journal_title：Glycobiology

authors： Berteau O,McCort I,Goasdoué N,Tissot B,Daniel R

更新日期：2002-04-01 00:00:00

abstract：:The γ-aminobutyric acid (GABA) transporters (GATs) have long been recognized for their key role in the uptake of neurotransmitters. The GAT1 belongs to the family of Na(+)- and Cl(-)-coupled transport proteins, which possess 12 putative transmembrane (TM) domains and three N-glycosylation sites on the extracellular lo...

journal_title：Glycobiology

authors： Hu J,Fei J,Reutter W,Fan H

更新日期：2011-03-01 00:00:00

abstract：:In this study, we report the first cloning and characterization of a N-acetylneuraminic acid phosphate synthase gene from Drosophila melanogaster, an insect in the protostome lineage. The gene is ubiquitously expressed at all stages of Drosophila development and in Schneider cells. Similar to the human homologue, the ...

journal_title：Glycobiology

authors： Kim K,Lawrence SM,Park J,Pitts L,Vann WF,Betenbaugh MJ,Palter KB

更新日期：2002-02-01 00:00:00

abstract：:Human milk oligosaccharides (HMOS) are not digested in the proximal intestine. In distal intestine, HMOS collectively modify the microbiota, but the response of individual bacteria to individual components of the HMOS is not well defined. Here, each of 25 major isolates of the human intestinal microbiota was fed indiv...

journal_title：Glycobiology

authors： Yu ZT,Chen C,Newburg DS

更新日期：2013-11-01 00:00:00

abstract：:Glycoprotein oligosaccharides function as tags for protein quality control in the endoplasmic reticulum (ER). Since most of proteins are glycosylated and function only after they are properly folded, glycoprotein glycan profiles in the ER might be useful to analyze various cellular status including diseases. Here, we ...

journal_title：Glycobiology

authors： Iwamoto S,Isoyama M,Hirano M,Yamaya K,Ito Y,Matsuo I,Totani K

更新日期：2013-01-01 00:00:00

abstract：:Presently almost no information is available on the oligosaccharide structure of the glycoproteins secreted from the methylotrophic yeast Hansenula polymorpha, a promising host for the production of recombinant proteins. In this study, we analyze the size distribution and structure of N-linked oligosaccharides attache...

journal_title：Glycobiology

authors： Kim MW,Rhee SK,Kim JY,Shimma Y,Chiba Y,Jigami Y,Kang HA

更新日期：2004-03-01 00:00:00

abstract：:Intestinal maturation can be influenced by intrinsic factors (glucocorticoid hormones) and by extrinsic factors (resident microflora); their relative roles in ontogeny of mouse intestinal trehalase expression, a marker of general gut development, and of beta1,4-galactosyltransferase (beta GT), a marker of glycosyltran...

journal_title：Glycobiology

authors： Nanthakumar NN,Dai D,Meng D,Chaudry N,Newburg DS,Walker WA

更新日期：2005-03-01 00:00:00

abstract：:N-Acetylglucosaminyltransferase-IV (GnT-IV) has two isoenzymes, GnT-IVa and GnT-IVb, which initiate the GlcNAcbeta1-4 branch synthesis on the Manalpha1-3 arm of the N-glycan core thereby increasing N-glycan branch complexity and conferring endogenous lectin binding epitopes. To elucidate the physiological significance...

journal_title：Glycobiology

authors： Takamatsu S,Antonopoulos A,Ohtsubo K,Ditto D,Chiba Y,Le DT,Morris HR,Haslam SM,Dell A,Marth JD,Taniguchi N

更新日期：2010-01-01 00:00:00

abstract：:Galactans are linear polysaccharides of β(1→4)-linked galactose residues. Although they can antagonize galectin function, the nature of their binding to galectins needs to be better defined to develop them as drugs. Here, we investigated interactions between galectin-3 (Gal-3) and a series of galactans ranging in weig...

journal_title：Glycobiology

authors： Miller MC,Zheng Y,Zhou Y,Tai G,Mayo KH

更新日期：2019-01-01 00:00:00

abstract：:Proliferation of exponentially growing breast cancer cells (line Hs578T) was blocked specifically in G1 by 3-hydroxy-3-methylglutaryl Coenzyme A (HMG CoA) reductase inhibition, as well as by inhibition of N-linked glycosylation. As a consequence of these inhibitory conditions, the cells were synchronized in the G1 sta...

journal_title：Glycobiology

authors： Larsson O

更新日期：1993-10-01 00:00:00

abstract：:The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transf...

journal_title：Glycobiology

authors： Chan NW,Stangier K,Sherburne R,Taylor DE,Zhang Y,Dovichi NJ,Palcic MM

更新日期：1995-10-01 00:00:00

abstract：:Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...

journal_title：Glycobiology

authors： Guarino C,DeLisa MP

更新日期：2012-05-01 00:00:00

abstract：:In the endoplasmic reticulum (ER), misfolded proteins are retrotranslocated to the cytosol and degraded by the proteasome in a process known as ER-associated degradation (ERAD). Early in this pathway, a proposed lumenal ER lectin, EDEM, recognizes misfolded glycoproteins in the ER, disengages the nascent molecules fro...

journal_title：Glycobiology

authors： Mast SW,Diekman K,Karaveg K,Davis A,Sifers RN,Moremen KW

更新日期：2005-04-01 00:00:00

abstract：:To investigate the developmental role of complex N-linked oligosaccharides, we previously inactivated the mouse Mgat1 gene which encodes UDP-N-acetylglucosamine: alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I (GlcNAc-TI). Mgat1-null embryos developed morphogenic abnormalities by embryonic day (E) 9.5 a...

journal_title：Glycobiology

authors： Campbell RM,Metzler M,Granovsky M,Dennis JW,Marth JD

更新日期：1995-07-01 00:00:00

abstract：:Human breast milk is an incredibly rich and complex biofluid composed of proteins, lipids and complex carbohydrates, including a diverse repertoire of free human milk oligosaccharides (HMOs). Strikingly, HMOs are not digested by the infant but function as prebiotics for bacterial strains associated with numerous benef...

journal_title：Glycobiology

authors： Porfirio S,Archer-Hartmann S,Moreau GB,Ramakrishnan G,Haque R,Kirkpatrick BD,Petri WA,Azadi P

更新日期：2020-09-28 00:00:00

abstract：:Erythropoietin (Epo) is a 166 amino acids protein containing three N-glycosylation sites (Asn-24, Asn-38, and Asn-83) and 1 O- glycosylation site (Ser-126) and involved in the regulation of the level of red blood cells. Today, only one recombinant human Epo (rHuEpo), produced in CHO cell line, is extensively used in t...

journal_title：Glycobiology

authors： Cointe D,Béliard R,Jorieux S,Leroy Y,Glacet A,Verbert A,Bourel D,Chirat F

更新日期：2000-05-01 00:00:00

abstract：:In this study, a sensitive, straightforward technique is developed for the analysis of glycoprotein O-linked oligosaccharides. Specifically, O-linked oligosaccharides of granulocyte colony-stimulating factor (G-CSF) are analysed by separating charged glycoforms using isoelectric focusing, electroblotting to polyvinyli...

journal_title：Glycobiology

authors： Andersen DC,Goochee CF,Cooper G,Weitzhandler M

更新日期：1994-08-01 00:00:00

abstract：:Alg3 of Saccharomyces cerevisiae catalyzes the mannosyl transfer from Man-P-Dol to Man(5)GlcNAc(2)-PP-Dol resulting in the formation of Man(6)GlcNAc(2)-PP-Dol, which is then further processed to the final precursor oligosaccharide Glc(3)Man(9)GlcNAc(2) for N-glycosylation of proteins. Here, we identified the alg3 gene...

journal_title：Glycobiology

authors： Berends E,Lehle L,Henquet M,Hesselink T,Wösten HA,Lugones LG,Bosch D

更新日期：2013-02-01 00:00:00

abstract：:A rapid and reproducible method was developed to detect and quantify carbohydrate-mediated cell adhesion to glycans arrayed on glass slides. Monosaccharides and oligosaccharides were covalently attached to glass slides in 1.7-mm-diameter spots (200 spots/slide) separated by a Teflon gasket. Primary chicken hepatocytes...

journal_title：Glycobiology

authors： Nimrichter L,Gargir A,Gortler M,Altstock RT,Shtevi A,Weisshaus O,Fire E,Dotan N,Schnaar RL

更新日期：2004-02-01 00:00:00

abstract：:Apicomplexan protozoa are a phylum of parasites that includes pathogens such as Plasmodium, the causative agent of the most severe form of malaria responsible for almost 1 million deaths per year and Toxoplasma gondii causing toxoplasmosis, a disease leading to cerebral meningitis in immunocompromised individuals or t...

journal_title：Glycobiology

authors： Debierre-Grockiego F,Schwarz RT

更新日期：2010-07-01 00:00:00

abstract：:Ganglioside GM3 (Siaα2-3Galβ1-4Glcβ1-1Cer) has been known to participate in insulin signaling by regulating the association of the insulin receptor in caveolae microdomains (lipid rafts), which is essential for the execution of the complete insulin metabolic signaling in adipocytes. Macrophage-secreted factors includi...

journal_title：Glycobiology

authors： Nagafuku M,Sato T,Sato S,Shimizu K,Taira T,Inokuchi J

更新日期：2015-03-01 00:00:00

abstract：:Lepidopteran insect cells are used routinely as hosts for foreign glycoprotein expression by recombinant baculoviruses, but the precise nature of their N-glycosylation pathway remains poorly defined. These cells clearly have processing glucosidases and mannosidases that can convert precursors to Man3GlcNAc2 structures...

journal_title：Glycobiology

authors： Jarvis DL,Bohlmeyer DA,Liao YF,Lomax KK,Merkle RK,Weinkauf C,Moremen KW

更新日期：1997-02-01 00:00:00

abstract：:The evolutionarily conserved Notch signaling pathway plays broad and important roles during embryonic development and in adult tissue homeostasis. Unlike most other pathways used during animal development, Notch signaling does not rely on second messengers and intracellular signaling cascades. Instead, pathway activat...

journal_title：Glycobiology

authors： Jafar-Nejad H,Leonardi J,Fernandez-Valdivia R

更新日期：2010-08-01 00:00:00

abstract：:To date, there is no complete structural characterization of human milk glycosaminoglycans (GAGs) available nor do any data exist on their composition in bovine milk. Total GAGs were determined on extracts from human and bovine milk. Samples were subjected to digestion with specific enzymes, treated with nitrous acid,...

journal_title：Glycobiology

authors： Coppa GV,Gabrielli O,Buzzega D,Zampini L,Galeazzi T,Maccari F,Bertino E,Volpi N

更新日期：2011-03-01 00:00:00

abstract：:Natural human interferon (IFN)-gamma has mainly biantennary complex-type sugar chains and scarcely has multiantennary structures. We attempted to remodel the sugar chain structures using IFN-gamma as a model glycoprotein. To obtain the branching glycoforms of IFN-gamma, we introduced the genes for GnT-IV (UDP-N-acetyl...

journal_title：Glycobiology

authors： Fukuta K,Abe R,Yokomatsu T,Kono N,Asanagi M,Omae F,Minowa MT,Takeuchi M,Makino T

更新日期：2000-04-01 00:00:00

abstract：:In cancer cells, the glycoprotein Mucin 1 (MUC1) undergoes abnormal, truncated glycosylation. The truncated glycosylation exposes cryptic peptide epitopes that can be recognized by antibodies. Since these immunogenic regions are cancer specific, they represent ideal targets for therapeutic antibodies. We investigated ...

journal_title：Glycobiology

authors： Movahedin M,Brooks TM,Supekar NT,Gokanapudi N,Boons GJ,Brooks CL

更新日期：2017-07-01 00:00:00

abstract：:We recently described a novel fluorescent compound, 2-amino,6-amidobiotinyl-pyridine (BAP), that allows the tagging of oligosaccharides, their fractionation by reversed-phase HPLC with picomole scale detection, and the formation of functional neoglycoprotein equivalents with (strept) avidin for the detection of recept...

journal_title：Glycobiology

authors： Toomre DK,Varki A

更新日期：1994-10-01 00:00:00

abstract：:Helicobacter pylori infects over half of the world's population and is thought to be a leading cause of gastric ulcer, gastric carcinoma, and gastric malignant lymphoma of mucosa-associated lymphoid tissue type. Previously, we reported that a gland mucin (MUC6) present in the lower portion of the gastric mucosa contai...

journal_title：Glycobiology

authors： Lee H,Wang P,Hoshino H,Ito Y,Kobayashi M,Nakayama J,Seeberger PH,Fukuda M

更新日期：2008-07-01 00:00:00

abstract：:Rabies glycoprotein (RGP(WT)) contains N-glycosylation sequons at Asn(37), Asn(247), and Asn(319), although Asn(37) is not efficiently glycosylated. To examine N-glycan processing at Asn(247) and Asn(319), full-length glycosylation mutants, RGP(-2-) and RGP(--3), were expressed, and Endo H sensitivity was compared. Wh...

journal_title：Glycobiology

authors： Wojczyk BS,Takahashi N,Levy MT,Andrews DW,Abrams WR,Wunner WH,Spitalnik SL

更新日期：2005-06-01 00:00:00

abstract：:Glycans mediate a wide variety of biological roles via recognition by glycan-binding proteins (GBPs). Comprehensive knowledge of such interaction is thus fundamental to glycobiology. While the primary binding feature of GBPs can be easily uncovered by using a simple glycan microarray harboring limited numbers of glyca...

journal_title：Glycobiology

authors： Li L,Guan W,Zhang G,Wu Z,Yu H,Chen X,Wang PG

更新日期：2020-04-20 00:00:00