Immunological reactions in response to apicomplexan glycosylphosphatidylinositols.

abstract：:Chinese hamster ovary (CHO) cells express only a subset of the glycosyltransferases activities known to exist. They do not express several fucosyltransferases, galactosyltransferases, sialyltransferases or N-acetylglucosaminyltransferases. However, following mutagenesis or transfection with large amounts of DNA, rare ...

journal_title：Glycobiology

authors： Stanley P,Raju TS,Bhaumik M

更新日期：1996-10-01 00:00:00

abstract：:A glycosyltransferase was fused to the yeast cell wall protein Pir, which forms the Pir1-4 protein family and is incorporated into the cell wall by an unknown linkage to be displayed at the yeast cell surface. We first expressed the PIR1-HA-gma12+ fusion, in which gma12+ encodes alpha-1,2-galactosyltransferase from th...

journal_title：Glycobiology

authors： Abe H,Shimma Y,Jigami Y

更新日期：2003-02-01 00:00:00

abstract：:Glycosyltransferases, usually residing within the intracellular secretory apparatus, also circulate in the blood. Many of these blood-borne glycosyltransferases are associated with pathological states, including malignancies and inflammatory conditions. Despite the potential for dynamic modifications of glycans on dis...

journal_title：Glycobiology

authors： Lee-Sundlov MM,Ashline DJ,Hanneman AJ,Grozovsky R,Reinhold VN,Hoffmeister KM,Lau JT

更新日期：2017-01-01 00:00:00

abstract：:The human parasitic trematode Schistosoma mansoni has a complex life cycle that includes the freshwater snail Biomphalaria glabrata as intermediate host. Within each stage, the parasite synthesizes a wide array of glycoconjugates, exhibiting, in part, unique carbohydrate structures. In addition, the parasite expresses...

journal_title：Glycobiology

authors： Lehr T,Geyer H,Maass K,Doenhoff MJ,Geyer R

更新日期：2007-01-01 00:00:00

abstract：:Saccharomyces cerevisiae Man9-alpha-mannosidase, responsible for trimming Man9GlcNAc2 in the endoplasmic reticulum to Man8GlcNAc2, the substrate for oligosaccharide elongation, has been purified to homogeneity from stabilized microsomal membranes without employing autolytic digestion. The activity was solubilized by t...

journal_title：Glycobiology

authors： Ziegler FD,Trimble RB

更新日期：1991-12-01 00:00:00

abstract：:The Malian medicinal plant Biophytum petersianum Klotzsch (Oxalidaceae) is used as a treatment against various types of illnesses related to the immune system, such as joint pains, inflammations, fever, malaria, and wounds. A pectic polysaccharide obtained from a hot water extract of the aerial parts of B. petersianum...

journal_title：Glycobiology

authors： Inngjerdingen M,Inngjerdingen KT,Patel TR,Allen S,Chen X,Rolstad B,Morris GA,Harding SE,Michaelsen TE,Diallo D,Paulsen BS

更新日期：2008-12-01 00:00:00

abstract：:Ceramide is a pivotal molecule in signal transduction and an essential structural component of the epidermal permeability barrier. The epidermis is marked by a high concentration of ceramide and by a unique spectrum of ceramide species: Besides the two ceramide structures commonly found in mammalian tissue, N-acylsphi...

journal_title：Glycobiology

authors： Vielhaber G,Brade L,Lindner B,Pfeiffer S,Wepf R,Hintze U,Wittern KP,Brade H

更新日期：2001-06-01 00:00:00

abstract：:Heparin/heparan sulfates (H/HS) are ubiquitous biopolymers that interact with many proteins to induce a range of biological functions. Unfortunately, how these biopolymers recognize their preferred protein targets remain poorly understood. It is suggested that computational simulations offer attractive avenues but a n...

journal_title：Glycobiology

authors： Nagarajan B,Sankaranarayanan NV,Desai UR

更新日期：2020-07-16 00:00:00

abstract：:Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are primarily localized in the plasma membrane. Cholera toxin B subunit (Ctxb), a component of a heat-labile enterotoxin produced by Vibrio cholerae, has been frequently used as a probe to detect GM1 ganglio...

journal_title：Glycobiology

authors： Yanagisawa M,Ariga T,Yu RK

更新日期：2006-09-01 00:00:00

abstract：:Sulfated O-linked oligosaccharides from the sea urchin egg receptor have been shown to bind to acrosome-reacted sperm and to inhibit fertilization in a competitive bioassay. However, the inhibitory activity of these isolated chains was much lower than that of a recombinant protein representing a portion of the extrace...

journal_title：Glycobiology

authors： Dhume ST,Stears RL,Lennarz WJ

更新日期：1996-01-01 00:00:00

abstract：:Presently almost no information is available on the oligosaccharide structure of the glycoproteins secreted from the methylotrophic yeast Hansenula polymorpha, a promising host for the production of recombinant proteins. In this study, we analyze the size distribution and structure of N-linked oligosaccharides attache...

journal_title：Glycobiology

authors： Kim MW,Rhee SK,Kim JY,Shimma Y,Chiba Y,Jigami Y,Kang HA

更新日期：2004-03-01 00:00:00

abstract：:Two different types of peptide:N-glycanase (PNGase) were identified in developing embryos of medaka fish ( Oryzias latipes ). Because the optimum pH values for their activities were acidic and neutral, they were designated as acid PNGase M and neutral PNGase M, respectively. The acid PNGase M corresponded to the enzym...

journal_title：Glycobiology

authors： Seko A,Kitajima K,Iwamatsu T,Inoue Y,Inoue S

更新日期：1999-09-01 00:00:00

abstract：:The mucin-type O-glycome in cancer aberrantly expresses the truncated glycans Tn (GalNAcα1-Ser/Thr) and STn (Neu5Acα2,6GalNAcα1-Ser/Thr). However, the role of Tn and STn in cancer and other diseases is not well understood. Our recent discovery of the self-binding properties (carbohydrate-carbohydrate interactions, CCI...

journal_title：Glycobiology

authors： Sletmoen M,Gerken TA,Stokke BT,Burchell J,Brewer CF

更新日期：2018-07-01 00:00:00

abstract：:Asparagine-linked (N-linked) protein glycosylation has been observed in all domains of life, including most recently in bacteria and is now widely considered a universal post-translational modification. However, cell-based production of homogeneous glycoproteins for laboratory and preparative purposes remains a signif...

journal_title：Glycobiology

authors： Guarino C,DeLisa MP

更新日期：2012-05-01 00:00:00

abstract：:Dendritic cell (DC) migration to secondary lymphoid organs is a critical step to properly exert its role in immunity and predominantly depends on the interaction of the chemokine receptor CCR7 with its ligands CCL21 and CCL19. Polysialic acid (PSA) has been recently reported to control CCL21-directed migration of matu...

journal_title：Glycobiology

authors： Rey-Gallardo A,Escribano C,Delgado-Martín C,Rodriguez-Fernández JL,Gerardy-Schahn R,Rutishauser U,Corbi AL,Vega MA

更新日期：2010-09-01 00:00:00

abstract：:Chitin oligosaccharides (COs) play a role in plant development and are presumed to affect body plan formation during vertebrate embryogenesis. The mechanisms of COs recognition and cellular processes underlying embryonic development are still not understood. We analyze the possible link with the mitogen-activated prot...

journal_title：Glycobiology

authors： Snaar-Jagalska BE,Krens SF,Robina I,Wang LX,Spaink HP

更新日期：2003-10-01 00:00:00

abstract：:Shigella flexneri is a gram-negative bacterium responsible for serious enteric infections that occur mainly in the terminal ileum and colon. High interest in Shigella, as a human pathogen, is driven by its antibiotic resistance and the necessity to develop a vaccine against its infections. Vaccines of the last generat...

journal_title：Glycobiology

authors： Molinaro A,Silipo A,Castro CD,Sturiale L,Nigro G,Garozzo D,Bernardini ML,Lanzetta R,Parrilli M

更新日期：2008-03-01 00:00:00

abstract：:The structure of the complex mixture of the core oligosaccharide components of the lipooligosaccharide (LOS) fraction of Agrobacterium tumefaciens strain TT111 was determined directly on the deacetylated products by means of spectroscopical methods. The rhamnan oligosaccharide elongating the inner Kdo residue shares s...

journal_title：Glycobiology

authors： De Castro C,Carannante A,Lanzetta R,Liparoti V,Molinaro A,Parrilli M

更新日期：2006-12-01 00:00:00

abstract：:At weaning, the intestinal mucosa surface glycans change from predominantly sialylated to fucosylated. Intestinal adaptation from milk to solid food is regulated by intrinsic and extrinsic factors. The contribution by glucocorticoid, an intrinsic factor, and colonization by microbiota, an extrinsic factor, was measure...

journal_title：Glycobiology

authors： Nanthakumar NN,Meng D,Newburg DS

更新日期：2013-10-01 00:00:00

abstract：:The human intestine houses a dense microbial ecosystem in which the struggle for nutrients creates a continual and dynamic selective force. Host-produced mucus glycans provide a ubiquitous source of carbon and energy for microbial species. Not surprisingly, many gut resident bacteria have become highly adapted to effi...

journal_title：Glycobiology

authors： Marcobal A,Southwick AM,Earle KA,Sonnenburg JL

更新日期：2013-09-01 00:00:00

abstract：:Schistosomiasis caused by infection with parasitic helminths of Schistosoma spp. is a major global health problem due to inadequate treatment and lack of a vaccine. The immune response to schistosomes includes glycan antigens, which could be valuable diagnostic markers and vaccine targets. However, no precedent exists...

journal_title：Glycobiology

authors： Prasanphanich NS,Luyai AE,Song X,Heimburg-Molinaro J,Mandalasi M,Mickum M,Smith DF,Nyame AK,Cummings RD

更新日期：2014-07-01 00:00:00

abstract：:We have recently demonstrated that the mannose-binding lectins, namely banana lectin (BL) and garlic lectin (GL), interacted with the insulin receptors on M210B4 cells--an established mesenchymal cell line of murine marrow origin--and initiate mitogen-activated protein kinase kinase (MEK)-dependent extracellular signa...

journal_title：Glycobiology

authors： Bajaj M,Hinge A,Limaye LS,Gupta RK,Surolia A,Kale VP

更新日期：2011-04-01 00:00:00

abstract：:The biosynthesis of HNK-1 carbohydrate is mainly regulated by two glucuronyltransferases (GlcAT-P and GlcAT-S) and a sulfotransferase (HNK-1 ST). To determine how the two glucuronyltransferases are involved in the biosynthesis of the HNK-1 carbohydrate, we prepared soluble forms of GlcAT-P and GlcAT-S fused with the I...

journal_title：Glycobiology

authors： Kakuda S,Sato Y,Tonoyama Y,Oka S,Kawasaki T

更新日期：2005-02-01 00:00:00

abstract：:A protocol is described for uniform 13C labelling of terminal galactose residues of the glycan chains of glycoproteins, using an enzymatic method which does not perturb the protein. The technique is illustrated by application to the biantennary N-linked glycan chains attached at Asn 297 of immunoglobulin G (IgG). Isot...

journal_title：Glycobiology

authors： Gilhespy-Muskett AM,Partridge J,Jefferis R,Homans SW

更新日期：1994-08-01 00:00:00

abstract：:We investigated the localization of major gangliosides in adult rat brain by an immunofluorescence technique with mouse monoclonal antibodies (MAbs). Five MAbs (GMB16, GMR17, GGR12, GMR5 and GMR13) that specifically recognize gangliosides GM1, GD1a, GD1b, GT1b and GQ1b, respectively, were used. We have found that ther...

journal_title：Glycobiology

authors： Kotani M,Kawashima I,Ozawa H,Terashima T,Tai T

更新日期：1993-04-01 00:00:00

abstract：:Aspartic protease, widely used as a milk-coagulating agent in industrial cheese production, contains three potential N-glycosylation sites. In this study, we report the characterization of N-linked oligosaccharides on recombinant aspartic protease secreted from the methylotrophic yeast Pichia pastoris using a combinat...

journal_title：Glycobiology

authors： Montesino R,Nimtz M,Quintero O,García R,Falcón V,Cremata JA

更新日期：1999-10-01 00:00:00

abstract：:Cancer-associated glycan structures can be both tumor markers and engines of disease progression. The structure Siaα2,6Galβ1,4GlcNAc (Sia6LacNAc), synthesized by sialyltransferase ST6GAL1, is a cancer-associated glycan. Although ST6GAL1/Sia6LacNAc are often overexpressed in colorectal cancer (CRC), their biological an...

journal_title：Glycobiology

authors： Venturi G,Gomes Ferreira I,Pucci M,Ferracin M,Malagolini N,Chiricolo M,Dall'Olio F

更新日期：2019-09-20 00:00:00

abstract：:Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was char...

journal_title：Glycobiology

authors： Pinto MR,Gorin PA,Wait R,Mulloy B,Barreto-Bergter E

更新日期：2005-10-01 00:00:00

abstract：:The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transf...

journal_title：Glycobiology

authors： Chan NW,Stangier K,Sherburne R,Taylor DE,Zhang Y,Dovichi NJ,Palcic MM

更新日期：1995-10-01 00:00:00

abstract：:The catabolism of dermatan sulfate (DS) commences with endohydrolysis of the polysaccharide to oligosaccharides by proposed endo-beta-N-acetylhexosaminidase and endohexuronidase activities. To investigate the substrate specificities of these activities, we developed an assay to measure specific products of their actio...

journal_title：Glycobiology

authors： Nielsen TC,Meikle PJ,Hopwood JJ,Fuller M

更新日期：2008-12-01 00:00:00