Abstract:
:Dendritic cell (DC) migration to secondary lymphoid organs is a critical step to properly exert its role in immunity and predominantly depends on the interaction of the chemokine receptor CCR7 with its ligands CCL21 and CCL19. Polysialic acid (PSA) has been recently reported to control CCL21-directed migration of mature DCs. Here, we first demonstrate that PSA present on human mature monocyte-derived dendritic cells did not enhance chemotactic responses to CCL19. We have also explored the molecular mechanisms underlying the selective enhancing effect of PSA on CCL21-driven chemotaxis of DCs. In this regard, we found out that prevention of DC polysialylation decreased CCL21 activation of JNK and Akt signaling pathways, both associated with CCR7-mediated chemotaxis. We also report that the enhanced PSA-mediated effect on DC migration towards CCL21 relied on the highly basic C-terminal region of this chemokine and depended on the PSA acceptor molecule neuropilin-2 (NRP2) and on the polysialyltransferase ST8SiaIV. Altogether, our data indicate that the CCR7/CCL21/NRP2/ST8SiaIV functional axis constitutes an important guidance clue for DC targeting to lymphoid organs.
journal_name
Glycobiologyjournal_title
Glycobiologyauthors
Rey-Gallardo A,Escribano C,Delgado-Martín C,Rodriguez-Fernández JL,Gerardy-Schahn R,Rutishauser U,Corbi AL,Vega MAdoi
10.1093/glycob/cwq078subject
Has Abstractpub_date
2010-09-01 00:00:00pages
1139-46issue
9eissn
0959-6658issn
1460-2423pii
cwq078journal_volume
20pub_type
杂志文章相关文献
GLYCOBIOLOGY文献大全abstract::Rabies glycoprotein (RGP(WT)) contains N-glycosylation sequons at Asn(37), Asn(247), and Asn(319), although Asn(37) is not efficiently glycosylated. To examine N-glycan processing at Asn(247) and Asn(319), full-length glycosylation mutants, RGP(-2-) and RGP(--3), were expressed, and Endo H sensitivity was compared. Wh...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwi046
更新日期:2005-06-01 00:00:00
abstract::Glycosaminoglycan (GAG) assembly initiates through the formation of a linkage tetrasaccharide region serving as a primer for both chondroitin sulfate (CS) and heparan sulfate (HS) chain polymerization. A possible role for sulfation of the linkage structure and of the constitutive disaccharide unit of CS chains in the ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwr172
更新日期:2012-04-01 00:00:00
abstract::An ultrasensitive method for analysis of polysialic acid (polySia) chains, using fluorescence-assisted high-performance liquid chromatography was developed. The new method is a substantial improvement of our earlier method in which the reducing terminal Sia residues of a homologous series of oligo/polySia hydrolytical...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.9.759
更新日期:2001-09-01 00:00:00
abstract::A rat pheochromocytoma cell line (PC12), when transfected with beta1,4-N-acetylglucosaminyltransferase III (GnT-III), which catalyzes the formation of a bisecting GlcNAc structure in N-glycans, resulted in the suppression of neurite outgrowth induced by costimulation of epidermal growth factor (EGF) and integrins. The...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh016
更新日期:2004-02-01 00:00:00
abstract::Cross-reactive carbohydrate determinants of plants are essentially a mixture of N-glycans containing beta1,2-xylose and core alpha1,3-fucose, the latter also found in insect glycoproteins. To determine the relative contributions of these two sugar residues to antibody binding, we prepared an array of glycomodified for...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwh058
更新日期:2004-05-01 00:00:00
abstract::Biosynthesis and functions of human milk oligosaccharides (HMO) are not well known. A typical housekeeping enzyme, beta1,4-galactosyltransferase, links galactose to glucose to form lactose which is then used as backbone for the assembly of HMO. We investigated whether milk lactose and HMO may be labeled in vivo by an ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj092
更新日期:2006-06-01 00:00:00
abstract::Invasive aspergillosis is an important cause of morbidity and mortality in immunocompromised patients. Diagnosis of this infection frequently employs detection of the circulating galactomannan in the patient serum using enzyme immunoassay (EIA), a highly sensitive and specific system. Although there are many structura...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu091
更新日期:2015-01-01 00:00:00
abstract::Human noroviruses (HuNoV), members of the family Caliciviridae, are the major cause of acute viral gastroenteritis worldwide. Successful infection is linked to the ability of the protruding (P) domain of the viral capsid to bind histo-blood group antigens (HBGA). Binding to gangliosides plays a major role for many non...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwx078
更新日期:2017-11-01 00:00:00
abstract::At weaning, the intestinal mucosa surface glycans change from predominantly sialylated to fucosylated. Intestinal adaptation from milk to solid food is regulated by intrinsic and extrinsic factors. The contribution by glucocorticoid, an intrinsic factor, and colonization by microbiota, an extrinsic factor, was measure...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwt050
更新日期:2013-10-01 00:00:00
abstract::The structure of the O-antigen polysaccharide (PS) of Escherichia coli O115 has been investigated using a combination of component analysis and 1D and 2D nuclear magnetic resonance (NMR) spectroscopy experiments. The repeating unit of the O-antigen was elucidated using the O-deacetylated PS and has the following branc...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws161
更新日期:2013-03-01 00:00:00
abstract::Phytohaemagglutinin (PHA) is the major lectin present in the seeds of the common bean, Phaseolus vulgaris, and PHA-L is the leucocyte-agglutinating form of this lectin. This tetrameric glycoprotein accumulates in the vacuoles of storage parenchyma cells. Based on amino acid sequence comparisons of legume lectins and t...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/3.6.581
更新日期:1993-12-01 00:00:00
abstract::Intelectin is an extracellular animal lectin found in chordata. Although human and mouse intelectin-1 recognize galactofuranosyl residues included in cell walls of various microorganisms, the physiological function of mammalian intelectin had been unclear. In this study, we found that human intelectin-1 was a serum pr...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwp013
更新日期:2009-05-01 00:00:00
abstract::Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these glycans is accumulating, their biosynthetic pathways are poorly understood. Here we report the identification and initial characterization of a novel enzyme capable of f...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/11.1.37
更新日期:2001-01-01 00:00:00
abstract::Rod and cone photoreceptors project from the outer retinal surface into a carbohydrate-rich interphotoreceptor matrix (IPM). Unique IPM glycoconjugates are distributed around rods and cones. Wheat germ agglutinin (WGA) strongly decorates the rod matrix domains and weakly decorates the cone matrix domains. This study c...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/8.10.997
更新日期:1998-10-01 00:00:00
abstract::Heparan sulfate 3-O-sulfotransferase transfers sulfate to the 3-OH position of a glucosamine to generate 3-O-sulfated heparan sulfate (HS), which is a rare component in HS from natural sources. We previously reported that 3-O- sulfotransferase isoform 5 (3-OST-5) generates both an antithrombin-binding site to exhibit ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwg101
更新日期:2003-11-01 00:00:00
abstract::Coccidioides spp. are important pneumonia-causing pathogens of the American southwest, but little is known about their glycobiology and how their glycosylations differ from other pneumonia-causing fungi. There is mounting preliminary evidence to suggest genus or even species-specific glycosylations in the fungal kingd...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwz092
更新日期:2020-02-19 00:00:00
abstract::It is demonstrated with glycopeptides of the polymorphic epithelial mucin (MUC1) that post-source decay matrix-assisted laser desorption ionization (PSD-MALDI) is a fast, highly sensitive, and reproducible method for the localization of O-glycosylation sites by reflectron time-of-flight (TOF) mass spectrometry. We hav...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.7.881
更新日期:1997-10-01 00:00:00
abstract::Cryptococcus neoformans, an opportunistic fungal pathogen, produces a glycan capsule to evade the immune system during infection. This definitive virulence factor is composed mainly of complex polysaccharides, which are made in the secretory pathway by reactions that utilize activated nucleotide sugar precursors. Alth...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww078
更新日期:2017-01-01 00:00:00
abstract::Glycosaminoglycans (GAGs) are long, anionic polysaccharides involved in many basic aspects of mammalian physiology and pathology. Here we describe a method to extract GAGs from formalin-fixed, paraffin-embedded tissues and found that they are structurally comparable with GAGs extracted from frozen tissues. We employed...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cws119
更新日期:2012-12-01 00:00:00
abstract::UDP-N-acetylglucosamine (UDP-GlcNAc) is a glucose metabolite with pivotal functions as a key substrate for the synthesis of glycoconjugates like hyaluronan, and as a metabolic sensor that controls cell functions through O-GlcNAc modification of intracellular proteins. However, little is known about the regulation of h...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cww019
更新日期:2016-07-01 00:00:00
abstract::Galectin-3 is a galactose-binding lectin that has been found in several mammalian tissues. Galectin-3 gene is expressed in a wide range of normal and tumoral cells. In the case of myeloid cells, its expression correlates with the differentiation of monocytes to macrophages. In the case of cancer cell lines, its expres...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.8.1089
更新日期:1997-12-01 00:00:00
abstract::The biosynthesis of the Lewis X determinant (Gal beta 1-4 [Fuc alpha 1-3]GlcNAc beta-) in three strains of Helicobacter pylori has been investigated. Strains UA 861, UA 802 and UA 1182 contain alpha 1,3 fucosyltransferase and beta 1,4 galactosyltransferase activities that synthesize the Lewis X structure by the transf...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/5.7.683
更新日期:1995-10-01 00:00:00
abstract::Heparan sulfate (HS) is a sulfated polysaccharide that plays a key role in morphogenesis, physiology and pathogenesis. The biosynthesis of HS takes place in the Golgi apparatus by a group of enzymes that polymerize, epimerize and sulfate the sugar chain. This biosynthetic process introduces varying degrees of sulfate ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwy055
更新日期:2018-11-01 00:00:00
abstract::A large family of UDP-GalNAc:polypeptide GalNAc transferases (ppGalNAc-Ts) initiates and defines sites of mucin-type Ser/Thr-O-GalNAc glycosylation. Family members have been classified into peptide- and glycopeptide-preferring subfamilies, although both families possess variable activities against glycopeptide substra...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwv108
更新日期:2016-04-01 00:00:00
abstract::The human hyaluronidase Hyal-1, one of six human hyaluronidase subtypes, preferentially degrades hyaluronic acid present in the extracellular matrix of somatic tissues. Modulations of Hyal-1 expression have been observed in a number of malignant tumors. However, its role in disease progression is discussed controversi...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm003
更新日期:2007-04-01 00:00:00
abstract::We have elucidated the carbohydrate-binding profile of a non-monosaccharide-binding lectin named Eucheuma serra lectin (ESA)-2 from the red alga Eucheuma serra using a lectin-immobilized column and a centrifugal ultrafiltration-high performance liquid chromatography method with a variety of fluorescence-labeled oligos...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwm007
更新日期:2007-05-01 00:00:00
abstract::Hypertrophy is central to several heart diseases; however, not much is known about the role of glycosphingolipids (GSLs) in this phenotype. Since GSLs have been accorded several physiological functions, we sought to determine whether these compounds affect cardiac hypertrophy. By using a rat cardiomyoblast cell line, ...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwu020
更新日期:2014-06-01 00:00:00
abstract::We have previously shown that chondroitin 6-sulfotransferase (C6ST) catalyzes transfer of sulfate not only to position 6 of GalNAc residue of chondroitin but also to position 6 of Gal residue of keratan sulfate. In this study, we examined the sulfation of sialyl lactosamine oligosaccharides by C6ST. C6ST catalyzed tra...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/7.3.405
更新日期:1997-04-01 00:00:00
abstract::Serglycin with a green fluorescent protein tag (SG-GFP) expressed in epithelial Madin-Darby canine kidney cells is secreted mainly (85%) into the apical medium, but the glycosaminoglycan (GAG) chains on the SG-GFP protein core secreted basolaterally (15%) carry most of the sulfate added during biosynthesis (Tveit et a...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/cwj075
更新日期:2006-04-01 00:00:00
abstract::Sialic acid has long been considered to be the sole receptor for influenza virus. The viral hemagglutinin (HA) is known to bind cell surface sialic acid, and sialic acids on viral glyco-proteins are cleaved by the viral neuraminidase (NA) to promote efficient release of progeny virus particles. However, NWS-Mvi, a mut...
journal_title:Glycobiology
pub_type: 杂志文章
doi:10.1093/glycob/10.7.649
更新日期:2000-07-01 00:00:00